LOCUS       sequence-372640-       10440 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MAC-tagged gene expression of human INSR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10440)
  AUTHORS   Salokas K, Liu X, Ohman T, Chowdhury I, Gawriyski L, Keskitalo S, 
            Varjosalo M
  TITLE     Physical and functional interactome atlas of human receptor tyrosine
            kinases.
  JOURNAL   EMBO Rep. 2022 Apr 5:e54041. doi: 10.15252/embr.202154041.
  PUBMED    35384245
REFERENCE   2  (bases 1 to 10440)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO Rep. 
            2022 Apr 5:e54041. doi: 10.15252/embr.202154041."
COMMENT     Sequence Label: MAC_C_INSR
FEATURES             Location/Qualifiers
     source          1..10440
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(47..151)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(226..245)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        417..796
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        797..1000
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     951..971
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    1002..1020
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1023..1041
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     1051..1075
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    1218..1242
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5359..5383)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5430..5453
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             5490..5513
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             5544..5570
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             5544..5570
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             join(5571..5918,5919..5921,5922..6530)
                     /codon_start=1
                     /product="promiscuous R118G mutant of the E. coli biotin 
                     protein ligase (Cronan, 2005)"
                     /label=BirA(R118G)
                     /note="also known as BirA* or BioID"
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKL
                     GADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGW
                     ITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGD
                     KEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     primer_bind     complement(6574..6591)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6580..6804
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6937..6956)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    7088..7135
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             7143..8165
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             7143..8165
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    8295..8416
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8332..8351)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8386..8405
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8465..8481)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8465..8481)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8478..8500)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8489..8505
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8513..8519,8520..8537,8538..8543))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8558..8579
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8696..8713)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8867..9455)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8947..8966)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9626..10417,10418..10440,1..46))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10249..10268
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
       61 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
      121 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
      181 tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga
      241 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg
      301 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct
      361 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca
      421 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
      481 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
      541 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
      601 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
      661 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
      721 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
      781 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
      841 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
      901 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
      961 cggtaggcgt gtacggtggg aggtctatat aagcagagct ctccctatca gtgatagaga
     1021 tctccctatc agtgatagag atcgtcgacg agctcgttta gtgaaccgtc agatcgcctg
     1081 gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat ccagcctccg
     1141 gactctagcg tttaaactta agcttggtac cgagctcgga tccactagtc cagtgtggtg
     1201 gaattctgca gatatcaaca agtttgtaca aaaaagcagg ctccaccatg ggcaccgggg
     1261 gccggcgggg ggcggcggcc gcgccgctgc tggtggcggt ggccgcgctg ctactgggcg
     1321 ccgcgggcca cctgtacccc ggagaggtgt gtcccggcat ggatatccgg aacaacctca
     1381 ctaggttgca tgagctggag aattgctctg tcatcgaagg acacttgcag atactcttga
     1441 tgttcaaaac gaggcccgaa gatttccgag acctcagttt ccccaaactc atcatgatca
     1501 ctgattactt gctgctcttc cgggtctatg ggctcgagag cctgaaggac ctgttcccca
     1561 acctcacggt catccgggga tcacgactgt tctttaacta cgcgctggtc atcttcgaga
     1621 tggttcacct caaggaactc ggcctctaca acctgatgaa catcacccgg ggttctgtcc
     1681 gcatcgagaa gaacaatgag ctctgttact tggccactat cgactggtcc cgtatcctgg
     1741 attccgtgga ggataattac atcgtgttga acaaagatga caacgaggag tgtggagaca
     1801 tctgtccggg taccgcgaag ggcaagacca actgccccgc caccgtcatc aacgggcagt
     1861 ttgtcgaacg atgttggact catagtcact gccagaaagt ttgcccgacc atctgtaagt
     1921 cacacggctg caccgccgaa ggcctctgtt gccacagcga gtgcctgggc aactgttctc
     1981 agcccgacga ccccaccaag tgcgtggcct gccgcaactt ctacctggac ggcaggtgtg
     2041 tggagacctg cccgcccccg tactaccact tccaggactg gcgctgtgtg aacttcagct
     2101 tctgccagga cctgcaccac aaatgcaaga actcgcggag gcagggctgc caccagtacg
     2161 tcattcacaa caacaagtgc atccctgagt gtccctccgg gtacacgatg aattccagca
     2221 acttgctgtg caccccatgc ctgggtccct gtcccaaggt gtgccacctc ctagaaggcg
     2281 agaagaccat cgactcggtg acgtctgccc aggagctccg aggatgcacc gtcatcaacg
     2341 ggagtctgat catcaacatt cgaggaggca acaatctggc agctgagcta gaagccaacc
     2401 tcggcctcat tgaagaaatt tcagggtatc taaaaatccg ccgatcctac gctctggtgt
     2461 cactttcctt cttccggaag ttacgtctga ttcgaggaga gaccttggaa attgggaact
     2521 actccttcta tgccttggac aaccagaacc taaggcagct ctgggactgg agcaaacaca
     2581 acctcaccat cactcagggg aaactcttct tccactataa ccccaaactc tgcttgtcag
     2641 aaatccacaa gatggaagaa gtttcaggaa ccaaggggcg ccaggagaga aacgacattg
     2701 ccctgaagac caatggggac caggcatcct gtgaaaatga gttacttaaa ttttcttaca
     2761 ttcggacatc ttttgacaag atcttgctga gatgggagcc gtactggccc cccgacttcc
     2821 gagacctctt ggggttcatg ctgttctaca aagaggcccc ttatcagaat gtgacggagt
     2881 tcgacgggca ggatgcgtgt ggttccaaca gttggacggt ggtagacatt gacccacccc
     2941 tgaggtccaa cgaccccaaa tcacagaacc acccagggtg gctgatgcgg ggtctcaagc
     3001 cctggaccca gtatgccatc tttgtgaaga ccctggtcac cttttcggat gaacgccgga
     3061 cctatggggc caagagtgac atcatttatg tccagacaga tgccaccaac ccctctgtgc
     3121 ccctggatcc aatctcagtg tctaactcat catcccagat tattctgaag tggaaaccac
     3181 cctccgaccc caatggcaac atcacccact acctggtttt ctgggagagg caggcggaag
     3241 acagtgagct gttcgagctg gattattgcc tcaaagggct gaagctgccc tcgaggacct
     3301 ggtctccacc attcgagtct gaagattctc agaagcacaa ccagagtgag tatgaggatt
     3361 cggccggcga atgctgctcc tgtccaaaga cagactctca gatcctgaag gagctggagg
     3421 agtcctcgtt taggaagacg tttgaggatt acctgcacaa cgtggttttc gtccccaggc
     3481 catctcggaa acgcaggtcc cttggcgatg ttgggaatgt gacggtggcc gtgcccacgg
     3541 tggcagcttt ccccaacact tcctcgacca gcgtgcccac gagtccggag gagcacaggc
     3601 cttttgagaa ggtggtgaac aaggagtcgc tggtcatctc cggcttgcga cacttcacgg
     3661 gctatcgcat cgagctgcag gcttgcaacc aggacacccc tgaggaacgg tgcagtgtgg
     3721 cagcctacgt cagtgcgagg accatgcctg aagccaaggc tgatgacatt gttggccctg
     3781 tgacgcatga aatctttgag aacaacgtcg tccacttgat gtggcaggag ccgaaggagc
     3841 ccaatggtct gatcgtgctg tatgaagtga gttatcggcg atatggtgat gaggagctgc
     3901 atctctgcgt ctcccgcaag cacttcgctc tggaacgggg ctgcaggctg cgtgggctgt
     3961 caccggggaa ctacagcgtg cgaatccggg ccacctccct tgcgggcaac ggctcttgga
     4021 cggaacccac ctatttctac gtgacagact atttagacgt cccgtcaaat attgcaaaaa
     4081 ttatcatcgg ccccctcatc tttgtctttc tcttcagtgt tgtgattgga agtatttatc
     4141 tattcctgag aaagaggcag ccagatgggc cgctgggacc gctttacgct tcttcaaacc
     4201 ctgagtatct cagtgccagt gatgtgtttc catgctctgt gtacgtgccg gacgagtggg
     4261 aggtgtctcg agagaagatc accctccttc gagagctggg gcagggctcc ttcggcatgg
     4321 tgtatgaggg caatgccagg gacatcatca agggtgaggc agagacccgc gtggcggtga
     4381 agacggtcaa cgagtcagcc agtctccgag agcggattga gttcctcaat gaggcctcgg
     4441 tcatgaaggg cttcacctgc catcatgtgg tgcgcctcct gggagtggtg tccaagggcc
     4501 agcccacgct ggtggtgatg gagctgatgg ctcacggaga cctgaagagc tacctccgtt
     4561 ctctgcggcc agaggctgag aataatcctg gccgccctcc ccctaccctt caagagatga
     4621 ttcagatggc ggcagagatt gctgacggga tggcctacct gaacgccaag aagtttgtgc
     4681 atcgggacct ggcagcgaga aactgcatgg tcgcccatga ttttactgtc aaaattggag
     4741 actttggaat gaccagagac atctatgaaa cggattacta ccggaaaggg ggcaagggtc
     4801 tgctccctgt acggtggatg gcaccggagt ccctgaagga tggggtcttc accacttctt
     4861 ctgacatgtg gtcctttggc gtggtccttt gggaaatcac cagcttggca gaacagcctt
     4921 accaaggcct gtctaatgaa caggtgttga aatttgtcat ggatggaggg tatctggatc
     4981 aacccgacaa ctgtccagag agagtcactg acctcatgcg catgtgctgg caattcaacc
     5041 ccaagatgag gccaaccttc ctggagattg tcaacctgct caaggacgac ctgcacccca
     5101 gctttccaga ggtgtcgttc ttccacagcg aggagaacaa ggctcccgag agtgaggagc
     5161 tggagatgga gtttgaggac atggagaatg tgcccctgga ccgttcctcg cactgtcaga
     5221 gggaggaggc ggggggccgg gatggagggt cctcgctggg tttcaagcgg agctacgagg
     5281 aacacatccc ttacacacac atgaacggag gcaagaaaaa cgggcggatt ctgaccttgc
     5341 ctcggtccaa tccttccgac ccagctttct tgtacaaagt ggttgatatc aaggccgccg
     5401 acatcaccag cctgtacaag aaagtggggt ggagccatcc ccagttcgag aagggcggag
     5461 gatctggcgg cggaagcggc ggaggaagct ggtcccaccc tcagtttgag aaaggcaccg
     5521 agctgggcag caccatggcc agctacccct acgacgtgcc cgactacgcc aaggacaaca
     5581 ccgtgcccct gaagctgatc gccctgctgg ccaacggcga gttccactct ggcgagcagc
     5641 tgggcgagac actgggcatg agcagagccg ccatcaacaa gcacatccag accctgcggg
     5701 actggggcgt ggacgtgttc acagtgcccg gcaagggcta cagcctgccc gagcccatcc
     5761 agctgctgaa cgccaagcag atcctgggcc agctggatgg cggatctgtg gccgtgctgc
     5821 ccgtgatcga cagcaccaac cagtatctgc tcgaccggat cggcgagctg aagtccggcg
     5881 acgcctgtat cgccgagtac cagcaggccg gcagaggcgg caggggcaga aagtggttca
     5941 gccccttcgg cgccaacctg tacctgagca tgttctggag actggaacag ggcccagccg
     6001 ccgctattgg cctgagcctg gtcatcggca tcgtgatggc cgaggtgctg cggaagctgg
     6061 gcgccgacaa agtgcgcgtg aagtggccca acgacctgta tctgcaggac cggaagctgg
     6121 ccggcatcct ggtggaactg accggcaaaa caggcgacgc cgcccagatc gtgatcggcg
     6181 ctggcatcaa catggccatg cggcgggtgg aagagagcgt ggtcaaccag ggctggatca
     6241 ccctgcagga agccggcatc aacctggacc ggaacaccct ggccgccatg ctgatcagag
     6301 agctgagagc cgccctggaa ctgttcgagc aggaaggcct ggccccctac ctgagcagat
     6361 gggagaagct ggacaacttc atcaaccggc ccgtgaaact gatcatcggc gacaaagaga
     6421 tcttcggcat cagccggggc atcgacaagc agggggccct gctcctggaa caggacggca
     6481 tcatcaagcc ctggatgggc ggcgagatca gcctgcggag cgccgagaag tgatgactcg
     6541 agtctagagg gcccgtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc
     6601 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
     6661 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
     6721 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg
     6781 ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg
     6841 ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     6901 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     6961 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     7021 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
     7081 gtacctagaa gttcctattc cgaagttcct attctctaga aagtatagga acttccttgg
     7141 ccaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa aagttcgaca
     7201 gcgtctccga cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc agcttcgatg
     7261 taggagggcg tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc tacaaagatc
     7321 gttatgttta tcggcacttt gcatcggccg cgctcccgat tccggaagtg cttgacattg
     7381 gggaattcag cgagagcctg acctattgca tctcccgccg tgcacagggt gtcacgttgc
     7441 aagacctgcc tgaaaccgaa ctgcccgctg ttctgcagcc ggtcgcggag gccatggatg
     7501 cgatcgctgc ggccgatctt agccagacga gcgggttcgg cccattcgga ccgcaaggaa
     7561 tcggtcaata cactacatgg cgtgatttca tatgcgcgat tgctgatccc catgtgtatc
     7621 actggcaaac tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct ctcgatgagc
     7681 tgatgctttg ggccgaggac tgccccgaag tccggcacct cgtgcacgcg gatttcggct
     7741 ccaacaatgt cctgacggac aatggccgca taacagcggt cattgactgg agcgaggcga
     7801 tgttcgggga ttcccaatac gaggtcgcca acatcttctt ctggaggccg tggttggctt
     7861 gtatggagca gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca ggatcgccgc
     7921 ggctccgggc gtatatgctc cgcattggtc ttgaccaact ctatcagagc ttggttgacg
     7981 gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc cgatccggag
     8041 ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg accgatggct
     8101 gtgtagaagt actcgccgat agtggaaacc gacgccccag cactcgtccg agggcaaagg
     8161 aatagcacgt actacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg
     8221 gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt
     8281 tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     8341 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     8401 tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat
     8461 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     8521 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     8581 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     8641 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     8701 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     8761 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     8821 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     8881 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     8941 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     9001 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     9061 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     9121 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     9181 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     9241 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     9301 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     9361 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     9421 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     9481 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     9541 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     9601 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     9661 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     9721 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     9781 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     9841 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     9901 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     9961 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
    10021 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
    10081 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
    10141 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
    10201 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
    10261 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
    10321 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
    10381 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
//
