LOCUS       sequence-37268-e         857 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 857)
  AUTHORS   Subach OM, Patterson GH, Ting LM, Wang Y, Condeelis JS, Verkhusha VV
  TITLE     A photoswitchable orange-to-far-red fluorescent protein, PSmOrange.
  JOURNAL   Nat Methods. 2011 Jul 31;8(9):771-7. doi: 10.1038/nmeth.1664.
  PUBMED    21804536
REFERENCE   2  (bases 1 to 857)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2011-07-31- 31"; volume: "8"; 
            issue: "9"; pages: "771-7"
COMMENT     Sequence Label: pH2B-PSmOrange
FEATURES             Location/Qualifiers
     source          1..857
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(125..835)
                     /codon_start=1
                     /product="orange to far-red photoswitchable variant of the 
                     monomeric fluorescent protein mOrange (Subach et al., 
                     2011)"
                     /label=PSmOrange
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEG
                     FQTAKLKVTKGGPLPFAWDILSPLFTYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNY
                     EDGGVVTVTQDSSLQDGEFIYKVKMRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIRMRLKLKDGGHYTSEVKTTYKAKKSVQLPGAYIVGIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(205..228)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     regulatory      832..841
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt
       61 ttttaaagca agtaaaacct ctacaaatgt ggtatggctg attatgatct agagtcgcgg
      121 ccgcttactt gtacagctcg tccatgccgc cggtggagtg gcggccctcg gcgcgttcgt
      181 actgttccac gatggtgtag tcctcgttgt gggaggtgat gtccagcttg atgccgacga
      241 tgtaggcgcc gggcagctgc acggacttct tggccttgta ggtggtcttg acctcggagg
      301 tgtagtggcc gccgtccttc agcttcagcc tcatcctgat ctcgcccttc agggcgccgt
      361 cctcggggta catccgctcg gaggaggcct cccagcccat ggtcttcttc tgcatcacgg
      421 ggccgtcgga ggggaagttg gtgccgcgca tcttcacctt gtagatgaac tcgccgtcct
      481 gcagtgagga gtcctgggtc acggtcacca cgccgccgtc ctcgtagttc atcacgcgct
      541 cccacttgaa gccctcgggg aaggacagct tgaagtagtc ggggatgtcg gcggggtgct
      601 tcacgtaggc cttggagccg taggtgaaga gaggggacag gatgtcccag gcgaagggca
      661 gggggccgcc cttggtcacc ttcagcttag cggtctgaaa gccctcgtag gggcggccct
      721 cgccctcgcc ctcgatctcg aactcgtggc cgttcacggt gccctccatg cgcaccttga
      781 agcgcatgaa ctccttgatg atggccatat tattctcctc gcccttgctc accatggtgg
      841 cgaccggtgg atcctta
//
