LOCUS       sequence-372723-        8922 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MAC-tagged gene expression of human LTK.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8922)
  AUTHORS   Salokas K, Liu X, Ohman T, Chowdhury I, Gawriyski L, Keskitalo S, 
            Varjosalo M
  TITLE     Physical and functional interactome atlas of human receptor tyrosine
            kinases.
  JOURNAL   EMBO Rep. 2022 Apr 5:e54041. doi: 10.15252/embr.202154041.
  PUBMED    35384245
REFERENCE   2  (bases 1 to 8922)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO Rep. 
            2022 Apr 5:e54041. doi: 10.15252/embr.202154041."
COMMENT     Sequence Label: MAC_C_LTK
FEATURES             Location/Qualifiers
     source          1..8922
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1770..1794)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1841..1864
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             1901..1924
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             1955..1981
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             1955..1981
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             join(1982..2329,2330..2332,2333..2941)
                     /codon_start=1
                     /product="promiscuous R118G mutant of the E. coli biotin 
                     protein ligase (Cronan, 2005)"
                     /label=BirA(R118G)
                     /note="also known as BirA* or BioID"
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKL
                     GADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGW
                     ITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGD
                     KEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     primer_bind     complement(2985..3002)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2991..3215
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(3348..3367)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    3499..3546
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             3554..4576
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             3554..4576
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    4706..4827
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4743..4762)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4797..4816
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4876..4892)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4876..4892)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4889..4911)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4900..4916
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4924..4930,4931..4948,4949..4954))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4969..4990
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5107..5124)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5278..5866)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5358..5377)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6037..6828,6829..6897))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6660..6679
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6898..7002)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7077..7096)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        7268..7647
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7648..7851
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7802..7822
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    7853..7871
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7874..7892
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     7902..7926
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    8069..8093
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 cggctggacg tcgcgggctc cctctccgca ggccggccgc tcactgcagg agggggcgga
       61 gggcggccag ggctgctccg aggcttgggc gacccttggc tgggccgcgg ccggcggctt
      121 cgggggcggc ggcggggcct gcactgcggg cggaggcggc ggcggctaca gggggggcga
      181 cgcttcagag actgacaacc tctgggctga tggggaagat ggagtatcct tcatacaccc
      241 cagcagcgag ctcttcctgc agcctctggc agtcaccgag aaccacggag aggtagagat
      301 ccgaaggcac ctcaactgca gtcactgccc tttgagagac tgccaatggc aggcagagct
      361 ccagctggct gaatgcctgt gcccagaagg catggagcta gctgtggata acgtcacctg
      421 catggacctg cacaagcccc caggccctct ggttctgatg gtggctgtgg tggcaacctc
      481 aacactgagc ctccttatgg tgtgtggggt cctgattctg gtgaagcaga agaagtggca
      541 gggcctgcag gagatgaggc tgccgagccc tgagcttgag ctgagcaagc ttcgaacctc
      601 tgccatcagg acagccccca atccctatta ttgccaggtg gggcttggcc cggcccagtc
      661 ctggcctctg ccaccaggtg tcaccgaggt ttccccagcc aatgttactc tgctcagagc
      721 cctgggccat ggtgcctttg gggaggtgta tgagggactg gtaattggcc ttcctgggga
      781 ctccagtccc ctgcaggtag ctatcaagac cctgccagaa ctctgctcgc ctcaggatga
      841 gctggatttc ctcatggagg ccctcatcat cagcaagttt cgccatcaga acattgtgcg
      901 gtgtgtgggg ctcagcctca gggccacccc tcgcctcatt ctgctggaac tgatgtctgg
      961 aggggacatg aagagtttcc tgaggcacag tcggccacac ctgggccagc catcacctct
     1021 ggtcatgcgg gacctgctgc aactggccca ggacatagcc cagggctgcc actacctgga
     1081 ggaaaatcac ttcatccaca gggatattgc cgcccggaac tgcctgctga gctgcgctgg
     1141 acccagccga gtggccaaga ttggggactt tgggatggca cgagatatct accgggccag
     1201 ttattaccgc aggggggacc gggccttgct cccagtcaag tggatgcccc cagaggcctt
     1261 cctggagggc atcttcacat ccaagacaga ttcctggtct tttggggtgc tgctctggga
     1321 gatcttctca ctgggctaca tgccctatcc tgggcgcacc aaccaggagg tgctggactt
     1381 cgtcgttgga ggaggccgga tggaccctcc taggggctgc ccagggcctg tgtaccgcat
     1441 catgacccag tgttggcagc acgagcctga gctccgccct agctttgcca gcatcttgga
     1501 gcgtctgcag tactgcactc aggacccgga tgtgctgaat tcactcctgc caatggagct
     1561 ggggcccacc ccagaggagg aagggacttc tgggctgggg aacagatctt tggagtgcct
     1621 aagaccccca cagccccagg aactgagtcc agagaagttg aaaagctggg gaggtagccc
     1681 tcttggcccc tggctgtcct ctggcctcaa gcccctcaaa tccaggggcc tccaacctca
     1741 gaacctttgg aatcccactt atcgctccga cccagctttc ttgtacaaag tggttgatat
     1801 caaggccgcc gacatcacca gcctgtacaa gaaagtgggg tggagccatc cccagttcga
     1861 gaagggcgga ggatctggcg gcggaagcgg cggaggaagc tggtcccacc ctcagtttga
     1921 gaaaggcacc gagctgggca gcaccatggc cagctacccc tacgacgtgc ccgactacgc
     1981 caaggacaac accgtgcccc tgaagctgat cgccctgctg gccaacggcg agttccactc
     2041 tggcgagcag ctgggcgaga cactgggcat gagcagagcc gccatcaaca agcacatcca
     2101 gaccctgcgg gactggggcg tggacgtgtt cacagtgccc ggcaagggct acagcctgcc
     2161 cgagcccatc cagctgctga acgccaagca gatcctgggc cagctggatg gcggatctgt
     2221 ggccgtgctg cccgtgatcg acagcaccaa ccagtatctg ctcgaccgga tcggcgagct
     2281 gaagtccggc gacgcctgta tcgccgagta ccagcaggcc ggcagaggcg gcaggggcag
     2341 aaagtggttc agccccttcg gcgccaacct gtacctgagc atgttctgga gactggaaca
     2401 gggcccagcc gccgctattg gcctgagcct ggtcatcggc atcgtgatgg ccgaggtgct
     2461 gcggaagctg ggcgccgaca aagtgcgcgt gaagtggccc aacgacctgt atctgcagga
     2521 ccggaagctg gccggcatcc tggtggaact gaccggcaaa acaggcgacg ccgcccagat
     2581 cgtgatcggc gctggcatca acatggccat gcggcgggtg gaagagagcg tggtcaacca
     2641 gggctggatc accctgcagg aagccggcat caacctggac cggaacaccc tggccgccat
     2701 gctgatcaga gagctgagag ccgccctgga actgttcgag caggaaggcc tggcccccta
     2761 cctgagcaga tgggagaagc tggacaactt catcaaccgg cccgtgaaac tgatcatcgg
     2821 cgacaaagag atcttcggca tcagccgggg catcgacaag cagggggccc tgctcctgga
     2881 acaggacggc atcatcaagc cctggatggg cggcgagatc agcctgcgga gcgccgagaa
     2941 gtgatgactc gagtctagag ggcccgttta aacccgctga tcagcctcga ctgtgccttc
     3001 tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc
     3061 cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg
     3121 tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa
     3181 tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg
     3241 gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     3301 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
     3361 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     3421 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
     3481 tgatggttca cgtacctaga agttcctatt ccgaagttcc tattctctag aaagtatagg
     3541 aacttccttg gccaaaaagc ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga
     3601 aaagttcgac agcgtctccg acctgatgca gctctcggag ggcgaagaat ctcgtgcttt
     3661 cagcttcgat gtaggagggc gtggatatgt cctgcgggta aatagctgcg ccgatggttt
     3721 ctacaaagat cgttatgttt atcggcactt tgcatcggcc gcgctcccga ttccggaagt
     3781 gcttgacatt ggggaattca gcgagagcct gacctattgc atctcccgcc gtgcacaggg
     3841 tgtcacgttg caagacctgc ctgaaaccga actgcccgct gttctgcagc cggtcgcgga
     3901 ggccatggat gcgatcgctg cggccgatct tagccagacg agcgggttcg gcccattcgg
     3961 accgcaagga atcggtcaat acactacatg gcgtgatttc atatgcgcga ttgctgatcc
     4021 ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc
     4081 tctcgatgag ctgatgcttt gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc
     4141 ggatttcggc tccaacaatg tcctgacgga caatggccgc ataacagcgg tcattgactg
     4201 gagcgaggcg atgttcgggg attcccaata cgaggtcgcc aacatcttct tctggaggcc
     4261 gtggttggct tgtatggagc agcagacgcg ctacttcgag cggaggcatc cggagcttgc
     4321 aggatcgccg cggctccggg cgtatatgct ccgcattggt cttgaccaac tctatcagag
     4381 cttggttgac ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt
     4441 ccgatccgga gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg
     4501 gaccgatggc tgtgtagaag tactcgccga tagtggaaac cgacgcccca gcactcgtcc
     4561 gagggcaaag gaatagcacg tactacgaga tttcgattcc accgccgcct tctatgaaag
     4621 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
     4681 catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata
     4741 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     4801 tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag
     4861 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     4921 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     4981 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     5041 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     5101 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     5161 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     5221 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     5281 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     5341 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     5401 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     5461 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     5521 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     5581 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     5641 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     5701 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     5761 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     5821 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     5881 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     5941 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     6001 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     6061 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     6121 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     6181 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     6241 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     6301 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     6361 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     6421 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     6481 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     6541 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     6601 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     6661 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     6721 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     6781 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     6841 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     6901 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     6961 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     7021 gccacctgac gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca
     7081 atctgctctg atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc
     7141 gctgagtagt gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc
     7201 atgaagaatc tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat
     7261 acgcgttgac attgattatt gactagttat taatagtaat caattacggg gtcattagtt
     7321 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
     7381 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
     7441 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
     7501 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
     7561 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
     7621 tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt
     7681 ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt
     7741 ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg
     7801 acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc tctccctatc
     7861 agtgatagag atctccctat cagtgataga gatcgtcgac gagctcgttt agtgaaccgt
     7921 cagatcgcct ggagacgcca tccacgctgt tttgacctcc atagaagaca ccgggaccga
     7981 tccagcctcc ggactctagc gtttaaactt aagcttggta ccgagctcgg atccactagt
     8041 ccagtgtggt ggaattctgc agatatcaac aagtttgtac aaaaaagcag gctccaccat
     8101 gggctgctgg ggacagctgc tggtgtggtt cggagccgcg ggcgccattc tctgctctag
     8161 cccggggtcc caggagactt ttctgcggtc ctcgcccctg ccgctggcaa gtcccagccc
     8221 ccgggacccg aaagtcagcg ccccgcctag tatcttggag ccagcctccc cgctgaattc
     8281 tccgggcacc gaggggtctt ggctgttttc tacctgcggg gccagcggcc ggcatgggcc
     8341 cacacagaca caatgtgacg gggcgtacgc ggggaccagc gtggtggtga ccgtgggggc
     8401 cgccgggcag ctgagaggcg tgcagctgtg gcgcgtgccg ggccctggcc agtatctgat
     8461 ctcagcctac ggagccgcgg gcggcaaagg cgccaagaac cacctgtcgc gggcgcatgg
     8521 cgtcttcgtc tcagcaatct tctccctcgg tctcggggag tcgctgtaca tcctggtggg
     8581 gcagcaggga gaggacgcct gtcccggagg tagcccggag agccagctcg tctgcctcgg
     8641 ggagtctcga gccgttgaag agcacgcggc gatggatggg agcgaagggg tcccggggtc
     8701 gcggcgctgg gcgggaggtg gcgggggtgg cgggggcgcc acctacgttt tccgggtgcg
     8761 cgctggcgag ctggaaccgt tgctggtggc ggccggaggc ggcggtcggg cctacctgag
     8821 gccgcgggac cgaggccgga ctcaggcctc ccccgagaaa ctggagaacc gctcggaggc
     8881 gcccgggagc ggcgggagag gcggggcggc aggtggtggg gg
//
