LOCUS       sequence-372724-        8514 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MAC-tagged gene expression of human EPHB6.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8514)
  AUTHORS   Salokas K, Liu X, Ohman T, Chowdhury I, Gawriyski L, Keskitalo S, 
            Varjosalo M
  TITLE     Physical and functional interactome atlas of human receptor tyrosine
            kinases.
  JOURNAL   EMBO Rep. 2022 Apr 5:e54041. doi: 10.15252/embr.202154041.
  PUBMED    35384245
REFERENCE   2  (bases 1 to 8514)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO Rep. 
            2022 Apr 5:e54041. doi: 10.15252/embr.202154041."
COMMENT     Sequence Label: MAC_C_EPHB6
FEATURES             Location/Qualifiers
     source          1..8514
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..46)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(200..788)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(280..299)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(959..1750,1751..1819))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1582..1601
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1820..1924)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1999..2018)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        2190..2569
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2570..2773
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2724..2744
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    2775..2793
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2796..2814
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2824..2848
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2991..3015
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5206..5230)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5277..5300
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             5337..5360
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             5391..5417
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             5391..5417
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             join(5418..5765,5766..5768,5769..6377)
                     /codon_start=1
                     /product="promiscuous R118G mutant of the E. coli biotin 
                     protein ligase (Cronan, 2005)"
                     /label=BirA(R118G)
                     /note="also known as BirA* or BioID"
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKL
                     GADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGW
                     ITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGD
                     KEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     primer_bind     complement(6421..6438)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6427..6651
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6784..6803)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    6935..6982
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             6990..8012
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             6990..8012
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    8142..8263
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8179..8198)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8233..8252
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8312..8328)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8312..8328)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8325..8347)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8336..8352
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8360..8366,8367..8384,8385..8390))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8405..8426
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
       61 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
      121 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
      181 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
      241 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
      301 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
      361 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
      421 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
      481 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
      541 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
      601 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
      661 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
      721 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
      781 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
      841 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
      901 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
      961 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     1021 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     1081 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     1141 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     1201 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     1261 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     1321 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     1381 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     1441 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     1501 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     1561 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     1621 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     1681 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     1741 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     1801 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     1861 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     1921 cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat ctcccgatcc
     1981 cctatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatctgct
     2041 ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag
     2101 gcaaggcttg accgacaatt gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct
     2161 tcgcgatgta cgggccagat atacgcgttg acattgatta ttgactagtt attaatagta
     2221 atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac
     2281 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac
     2341 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt
     2401 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat
     2461 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
     2521 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt
     2581 ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca
     2641 ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg
     2701 tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta
     2761 tataagcaga gctctcccta tcagtgatag agatctccct atcagtgata gagatcgtcg
     2821 acgagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct
     2881 ccatagaaga caccgggacc gatccagcct ccggactcta gcgtttaaac ttaagcttgg
     2941 taccgagctc ggatccacta gtccagtgtg gtggaattct gcagatatca acaagtttgt
     3001 acaaaaaagt tggcaccatg gtagctgtcg ggggctgccg ctgccagcct ggataccaac
     3061 cagcacgagg agacaaggcc tgccaagcct gcccacgggg gctctataag tcttctgctg
     3121 ggaatgctcc ctgctcacca tgccctgccc gcagtcacgc tcccaaccca gcagcccccg
     3181 tttgcccctg cctggagggc ttctaccggg ccagttccga cccaccagag gccccctgca
     3241 ctggtcctcc atcggctccc caggagcttt ggtttgaggt gcaaggctca gcactcatgc
     3301 tacactggcg cctgcctcgg gagctggggg gtcgagggga cctgctcttc aatgtcgtgt
     3361 gcaaggagtg tgaaggccgc caggaacctg ccagcggtgg tgggggcact tgtcaccgct
     3421 gcagggatga ggtccacttc gaccctcgcc agagaggcct gactgagagc cgagtgttag
     3481 tggggggact ccgggcacac gtaccctaca tcttagaggt gcaggctgtt aatggggtgt
     3541 ctgagctcag ccctgaccct cctcaggctg cagccatcaa tgtcagcacc agccatgaag
     3601 tgccctctgc tgtccctgtg gtgcaccagg tgagccgggc atccaacagc atcacggtgt
     3661 cctggccgca gcccgaccag accaatggga acatcctgga ctatcagctc cgctactatg
     3721 accaggcaga agacgaatcc cactccttca ccctgaccag cgagaccaac actgccaccg
     3781 tgacacagct gagccctggc cacatctatg gtttccaggt gcgggcccgg actgctgccg
     3841 gccacggccc ctacgggggc aaagtctatt tccagacact tcctcaaggg gagctgtctt
     3901 cccagcttcc ggaaagactc tccttggtga tcggctccat cctgggggct ttggccttcc
     3961 tcctgctggc agccatcacc gtgctggcgg tcgtcttcca gcggaagcgg cgtgggactg
     4021 gctacacgga gcagctgcag caatacagca gcccaggact cggggtgaag tattacatcg
     4081 acccctccac ctacgaggac ccctgtcagg ccatccgaga acttgcccgg gaagtcgatc
     4141 ctgcttatat caagattgag gaggtcattg ggacaggctc ttttggagaa gtgcgccagg
     4201 gccgcctgca gccacgggga cggagggagc agactgtggc catccaggcc ctgtgggccg
     4261 ggggcgccga aagcctgcag atgaccttcc tgggccgggc cgcagtgctg ggtcagttcc
     4321 agcaccccaa catcctgcgg ctggagggcg tggtcaccaa gagccgaccc ctcatggtgc
     4381 tgacggagtt catggagctt ggccccctgg acagcttcct caggcagcgg gagggccagt
     4441 tcagcagcct gcagctggtg gccatgcagc ggggagtggc tgctgccatg cagtacctgt
     4501 ccagctttgc cttcgtccat cgctcgctgt ctgcccacag cgtgctggtg aatagccact
     4561 tggtgtgcaa ggtggcccgt cttggccaca gtcctcaggg cccaagttgt ttgcttcgct
     4621 gggcagcccc agaggtcatt gcacatggaa agcatacaac atccagtgat gtctggagct
     4681 ttgggatact catgtgggaa gtgatgagtt atggagaacg gccttactgg gacatgagtg
     4741 agcaggaggt actaaatgca atagagcagg agttccggct gcccccgcct ccaggctgtc
     4801 ctcctggatt acatctactt atgttggaca cttggcagaa ggaccgtgcc cggcggcctc
     4861 attttgacca gctggtggct gcatttgaca agatgatccg caagccagat accctgcagg
     4921 ctggcgggga cccaggggaa aggccttccc aggcccttct gacccctgtg gccctggact
     4981 ttccttgtct ggactcaccc caggcctggc tttcagccat tggactggag tgctaccagg
     5041 acaacttctc caagtttggc ctctgtacct tcagtgatgt ggctcagctc agcctagaag
     5101 acctgcctgc cctgggcatc accctggctg gccaccagaa gaagctgctg caccacatcc
     5161 agctccttca gcaacacctg aggcagcagg gctcagtgga ggtcttgcca actttcttgt
     5221 acaaagtggt tgatatcaag gccgccgaca tcaccagcct gtacaagaaa gtggggtgga
     5281 gccatcccca gttcgagaag ggcggaggat ctggcggcgg aagcggcgga ggaagctggt
     5341 cccaccctca gtttgagaaa ggcaccgagc tgggcagcac catggccagc tacccctacg
     5401 acgtgcccga ctacgccaag gacaacaccg tgcccctgaa gctgatcgcc ctgctggcca
     5461 acggcgagtt ccactctggc gagcagctgg gcgagacact gggcatgagc agagccgcca
     5521 tcaacaagca catccagacc ctgcgggact ggggcgtgga cgtgttcaca gtgcccggca
     5581 agggctacag cctgcccgag cccatccagc tgctgaacgc caagcagatc ctgggccagc
     5641 tggatggcgg atctgtggcc gtgctgcccg tgatcgacag caccaaccag tatctgctcg
     5701 accggatcgg cgagctgaag tccggcgacg cctgtatcgc cgagtaccag caggccggca
     5761 gaggcggcag gggcagaaag tggttcagcc ccttcggcgc caacctgtac ctgagcatgt
     5821 tctggagact ggaacagggc ccagccgccg ctattggcct gagcctggtc atcggcatcg
     5881 tgatggccga ggtgctgcgg aagctgggcg ccgacaaagt gcgcgtgaag tggcccaacg
     5941 acctgtatct gcaggaccgg aagctggccg gcatcctggt ggaactgacc ggcaaaacag
     6001 gcgacgccgc ccagatcgtg atcggcgctg gcatcaacat ggccatgcgg cgggtggaag
     6061 agagcgtggt caaccagggc tggatcaccc tgcaggaagc cggcatcaac ctggaccgga
     6121 acaccctggc cgccatgctg atcagagagc tgagagccgc cctggaactg ttcgagcagg
     6181 aaggcctggc cccctacctg agcagatggg agaagctgga caacttcatc aaccggcccg
     6241 tgaaactgat catcggcgac aaagagatct tcggcatcag ccggggcatc gacaagcagg
     6301 gggccctgct cctggaacag gacggcatca tcaagccctg gatgggcggc gagatcagcc
     6361 tgcggagcgc cgagaagtga tgactcgagt ctagagggcc cgtttaaacc cgctgatcag
     6421 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct
     6481 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc
     6541 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg
     6601 aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg
     6661 cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa
     6721 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     6781 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     6841 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     6901 aaaaacttga ttagggtgat ggttcacgta cctagaagtt cctattccga agttcctatt
     6961 ctctagaaag tataggaact tccttggcca aaaagcctga actcaccgcg acgtctgtcg
     7021 agaagtttct gatcgaaaag ttcgacagcg tctccgacct gatgcagctc tcggagggcg
     7081 aagaatctcg tgctttcagc ttcgatgtag gagggcgtgg atatgtcctg cgggtaaata
     7141 gctgcgccga tggtttctac aaagatcgtt atgtttatcg gcactttgca tcggccgcgc
     7201 tcccgattcc ggaagtgctt gacattgggg aattcagcga gagcctgacc tattgcatct
     7261 cccgccgtgc acagggtgtc acgttgcaag acctgcctga aaccgaactg cccgctgttc
     7321 tgcagccggt cgcggaggcc atggatgcga tcgctgcggc cgatcttagc cagacgagcg
     7381 ggttcggccc attcggaccg caaggaatcg gtcaatacac tacatggcgt gatttcatat
     7441 gcgcgattgc tgatccccat gtgtatcact ggcaaactgt gatggacgac accgtcagtg
     7501 cgtccgtcgc gcaggctctc gatgagctga tgctttgggc cgaggactgc cccgaagtcc
     7561 ggcacctcgt gcacgcggat ttcggctcca acaatgtcct gacggacaat ggccgcataa
     7621 cagcggtcat tgactggagc gaggcgatgt tcggggattc ccaatacgag gtcgccaaca
     7681 tcttcttctg gaggccgtgg ttggcttgta tggagcagca gacgcgctac ttcgagcgga
     7741 ggcatccgga gcttgcagga tcgccgcggc tccgggcgta tatgctccgc attggtcttg
     7801 accaactcta tcagagcttg gttgacggca atttcgatga tgcagcttgg gcgcagggtc
     7861 gatgcgacgc aatcgtccga tccggagccg ggactgtcgg gcgtacacaa atcgcccgca
     7921 gaagcgcggc cgtctggacc gatggctgtg tagaagtact cgccgatagt ggaaaccgac
     7981 gccccagcac tcgtccgagg gcaaaggaat agcacgtact acgagatttc gattccaccg
     8041 ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc
     8101 tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt
     8161 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     8221 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt
     8281 cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     8341 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     8401 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     8461 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcg
//
