LOCUS       sequence-372730-        6447 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express IL2-AH-mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6447)
  AUTHORS   Budnar S, Husain KB, Gomez GA, Naghibosadat M, Varma A, Verma S, 
            Hamilton NA, Morris RG, Yap AS
  TITLE     Anillin Promotes Cell Contractility by Cyclic Resetting of RhoA 
            Residence Kinetics
  JOURNAL   Dev Cell. 2019 Jun 17;49(6):894-906.e12. doi: 
            10.1016/j.devcel.2019.04.031. Epub 2019 May 16.
  PUBMED    31105010
REFERENCE   2  (bases 1 to 6447)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2019.04.031"; journalName: "Dev Cell"; date: 
            "2019-06-17- 17"; volume: "49"; issue: "6"; pages: "894-906.e12"
COMMENT     Sequence Label: IL2-mCherry-AH in pmCherryN1
FEATURES             Location/Qualifiers
     source          1..6447
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        68..371
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        372..575
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     526..546
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      2403..2412
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(2554..2572)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2822..2841
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    3242..3363
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3279..3298)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3333..3352
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3370..3825)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3507..3528)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3719..3738
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3852..3956
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3958..4315
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3958..4149
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3981..4001)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4166..4301
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4350..5144
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4350..5144
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4404..4423)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5014..5033
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5332..5351)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5376..5423
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5752..6340
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6241..6260
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 catgcattag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
       61 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
      121 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
      181 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
      241 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
      301 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      361 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
      421 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
      481 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
      541 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
      601 agatggattc atacctgctg atgtggggac tgctcacgtt catcatggtg cctggctgcc
      661 aggcagagct ctgtgacgat gacccgccag agatcccaca cgccacattc aaagccatgg
      721 cctacaagga aggaaccatg ttgaactgtg aatgcaagag aggtttccgc agaataaaaa
      781 gcgggtcact ctatatgctc tgtacaggaa actctagcca ctcgtcctgg gacaaccaat
      841 gtcaatgcac aagctctgcc actcggaaca caacgaaaca agtgacacct caacctgaag
      901 aacagaaaga aaggaaaacc acagaaatgc aaagtccaat gcagccagtg gaccaagcga
      961 gccttccagg tcactgcagg gaacctccac catgggaaaa tgaagccaca gagagaattt
     1021 atcatttcgt ggtggggcag atggtttatt atcagtgcgt ccagggatac agggctctac
     1081 acagaggtcc tgctgagagc gtctgcaaaa tgacccacgg gaagacaagg tggacccagc
     1141 cccagctcat atgcacaggt gaaatggaga ccagtcagtt tccaggtgaa gagaagcctc
     1201 aggcaagccc cgaaggccgt cctgagagtg agacttcctg cctcgtcaca acaacagatt
     1261 ttcaaataca gacagaaatg gctgcaacca tggagacgtc catatttaca acagagtacc
     1321 aggtagcagt ggccggctgt gttttcctgc tgatcagcgt cctcctcctg agtgggctca
     1381 cctggaaggg aaaattccaa agaactcgtg tccctcgagc tgaatctggt gatagccttg
     1441 gttctgaaga tcgtgatctt ctttacagca ttgatgcata tagatctcaa agattcaaag
     1501 aaacagaacg tccatcaata aagcaggtga ttgttcggaa ggaagatgtt acttcaaaac
     1561 tggatgaaaa aaataatgcc tttccttgtc aagttaatat caaacagaaa atgcaggaac
     1621 tcaataacga aataaatatg caacagacag tgatctatca agctagccag gctcttaact
     1681 gctgtgttga tgaagaacat ggaaaagggt ccctagaaga agctgaagca gaaagacttc
     1741 ttctaattgc aactgggaag agaacacttt tgattgatga attgaataaa ttgaagaacg
     1801 aaggacctca gaggaagaat aaggctagtc cccaaagtga atttatgcca tccaaaggat
     1861 cagttacttt gtcagaaatc cgcttgcctc taaaagcaga ttttgtctgc agtacggttc
     1921 agaaaccaga tgcagcaaat tactattact taattatact aaaagcagga gctgaaaata
     1981 tggtagccac accattagca agtacttcaa actctcttaa cggtgatgct ctgacattca
     2041 ctactacatt tactctgcaa gatgtatcca atgactttga aataaatatt gaagtttaca
     2101 gcttggtgca aaagaaagat ccctcaggcc ttgataagaa gaaaaaaaca tccaagtcca
     2161 aggctattac tccaaagcga ctcctcacat ctataaccac aaaaagcaac attcattctt
     2221 cagtcatggc cagtccagga ggtcttagtg ctgtgcgaac cagcaacttc gcccttgttg
     2281 gatcttacac attatcattg tcttcagtag gaaatactaa gtttgttctg gacaaggtcc
     2341 cctttttatc ttctttggaa ggtcatattt atttaaaaat aaaatgtcaa gtgcgaccgg
     2401 tcgccaccat ggtgagcatg gacgaggagg ataacatggc catcatcaag gagttcatgc
     2461 gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc gagggcgagg
     2521 gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggtggcc
     2581 ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc aaggcctacg
     2641 tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag ggcttcaagt
     2701 gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctccc
     2761 tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg
     2821 gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg atgtaccccg
     2881 aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac ggcggccact
     2941 acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct
     3001 acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac
     3061 agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaagtaaa
     3121 gcggccgcga ctctagatca taatcagcca taccacattt gtagaggttt tacttgcttt
     3181 aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt
     3241 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     3301 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     3361 ttaaggcgta aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa
     3421 tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat
     3481 agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg
     3541 tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac
     3601 catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta
     3661 aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag
     3721 ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg
     3781 taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtcaggt ggcacttttc
     3841 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     3901 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtcctg
     3961 aggcggaaag aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
     4021 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
     4081 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     4141 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     4201 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     4261 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaagatcg
     4321 atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt
     4381 ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct
     4441 gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga
     4501 ccgacctgtc cggtgccctg aatgaactgc aagacgaggc agcgcggcta tcgtggctgg
     4561 ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact
     4621 ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg
     4681 agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct
     4741 gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg
     4801 gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt
     4861 tcgccaggct caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg
     4921 cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc
     4981 ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag
     5041 agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt
     5101 cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt
     5161 cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc
     5221 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca
     5281 gcgcggggat ctcatgctgg agttcttcgc ccaccctagg gggaggctaa ctgaaacacg
     5341 gaaggagaca ataccggaag gaacccgcgc tatgacggca ataaaaagac agaataaaac
     5401 gcacggtgtt gggtcgtttg ttcataaacg cggggttcgg tcccagggct ggcactctgt
     5461 cgatacccca ccgagacccc attggggcca atacgcccgc gtttcttcct tttccccacc
     5521 ccacccccca agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg cggcaggccc
     5581 tgccatagcc tcaggttact catatatact ttagattgat ttaaaacttc atttttaatt
     5641 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     5701 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     5761 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     5821 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     5881 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     5941 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     6001 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     6061 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     6121 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     6181 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     6241 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     6301 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     6361 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     6421 tgattctgtg gataaccgta ttaccgc
//
