LOCUS       sequence-37274-e         885 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 885)
  AUTHORS   Valastyan JS, Lindquist S
  TITLE     TorsinA and the TorsinA-Interacting Protein Printor Have No Impact 
            on Endoplasmic Reticulum Stress or Protein Trafficking in Yeast.
  JOURNAL   PLoS One. 2011;6(7):e22744. Epub 2011 Jul 26.
  PUBMED    21818381
REFERENCE   2  (bases 1 to 885)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(7)"; pages: "e22744. Epub 2011 Jul 26"
COMMENT     Sequence Label: 416-Met25 Kar2 printor HDEL
FEATURES             Location/Qualifiers
     source          1..885
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        69..418
                     /gene="S. cerevisiae MET17"
                     /gene_synonym="MET15; MET25"
                     /label=MET17 promoter
                     /note="expression is repressed by methionine"
     primer_bind     419..435
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     419..435
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     protein_bind    443..467
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 agggcattac ctcctaaggg acaaaagctg gagctccgga tgcaagggtt cgaatccctt
       61 agctctcatt attttttgct ttttctcttg aggtcacatg atcgcaaaat ggcaaatggc
      121 acgtgaagct gtcgatattg gggaactgtg gtggttggca aatgactaat taagttagtc
      181 aaggcgccat cctcatgaaa actgtgtaac ataataaccg aagtgtcgaa aaggtggcac
      241 cttgtccaat tgaacacgct cgatgaaaaa aataagatat atataaggtt aagtaaagcg
      301 tctgttagaa aggaagtttt tcctttttct tgctctcttg tcttttcatc tactatttcc
      361 ttcgtgtaat acagggtcgt cagatacata gatacaattc tattaccccc atccatactc
      421 tagaactagt ggatccccca tcacaagttt gtacaaaaaa gcaggcttca caaaatgttt
      481 ttcaacagac taagcgctgg caagctgctg gtaccactct ccgtggtcct gtacgccctt
      541 ttcgtggtaa tattaccttt acagaattct ttccactcct ccaatgtttt agttagaggt
      601 atgtccagat ccggggacag gacctccacc ttcgacccca gccacagcga caacctgctg
      661 cacggcctca acctgctgtg gaggaagcag ctgttttgcg acgtgaccct gacggcccag
      721 ggccagcagt tccattgcca caaggccgtg ctggcctcct gctcgcagta cttccgatcg
      781 ctcttctcca gccacccccc tctcggggga ggggtcggcg gccaggacgg cctgggggcc
      841 cccaaggacc agcagcagcc gccgcagcag cagccgtcca cacac
//
