LOCUS       sequence-372764-        9254 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pET21a protein expression vector for 1N/2CxNLS-cMyc-NLP-cMyc 
            LbaCas12a in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9254)
  AUTHORS   Luk K, Liu P, Zeng J, Wang Y, Maitland SA, Idrizi F, Ponnienselvan 
            K, Iyer S, Zhu LJ, Luban J, Bauer DE, Wolfe SA
  TITLE     Optimization of Nuclear Localization Signal Composition Improves 
            CRISPR-Cas12a Editing Rates in Human Primary Cells.
  JOURNAL   GEN Biotechnol. 2022 Jun;1(3):271-284. doi: 
            10.1089/genbio.2022.0003. Epub 2022 Jun 14.
  PUBMED    38405215
REFERENCE   2  (bases 1 to 9254)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1089/genbio.2022.0003"; journalName: "GEN Biotechnol"; date: 
            "2022-06"; volume: "1"; issue: "3"; pages: "271-284"
COMMENT     Sequence Label: 1N/2CxNLS-cMyc-NLP-cMyc LbaCas12a
FEATURES             Location/Qualifiers
     source          1..9254
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     81..100
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     184..203
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        184..202
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    203..227
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             242..264
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             275..301
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             320..4000
                     /codon_start=1
                     /product="CRISPR-associated protein from Lachnospiraceae 
                     bacterium ND2006 (Zetsche et al., 2015)"
                     /label=LbCpf1
                     /translation="SKLEKFTNCYSLSKTLRFKAIPVGKTQENIDNKRLLVEDEKRAED
                     YKGVKKLLDRYYLSFINDVLHSIKLKNLNNYISLFRKKTRTEKENKELENLEINLRKEI
                     AKAFKGNEGYKSLFKKDIIETILPEFLDDKDEIALVNSFNGFTTAFTGFFDNRENMFSE
                     EAKSTSIAFRCINENLTRYISNMDIFEKVDAIFDKHEVQEIKEKILNSDYDVEDFFEGE
                     FFNFVLTQEGIDVYNAIIGGFVTESGEKIKGLNEYINLYNQKTKQKLPKFKPLYKQVLS
                     DRESLSFYGEGYTSDEEVLEVFRNTLNKNSEIFSSIKKLEKLFKNFDEYSSAGIFVKNG
                     PAISTISKDIFGEWNVIRDKWNAEYDDIHLKKKAVVTEKYEDDRRKSFKKIGSFSLEQL
                     QEYADADLSVVEKLKEIIIQKVDEIYKVYGSSEKLFDADFVLEKSLKKNDAVVAIMKDL
                     LDSVKSFENYIKAFFGEGKETNRDESFYGDFVLAYDILLKVDHIYDAIRNYVTQKPYSK
                     DKFKLYFQNPQFMGGWDKDKETDYRATILRYGSKYYLAIMDKKYAKCLQKIDKDDVNGN
                     YEKINYKLLPGPNKMLPKVFFSKKWMAYYNPSEDIQKIYKNGTFKKGDMFNLNDCHKLI
                     DFFKDSISRYPKWSNAYDFNFSETEKYKDIAGFYREVEEQGYKVSFESASKKEVDKLVE
                     EGKLYMFQIYNKDFSDKSHGTPNLHTMYFKLLFDENNHGQIRLSGGAELFMRRASLKKE
                     ELVVHPANSPIANKNPDNPKKTTTLSYDVYKDKRFSEDQYELHIPIAINKCPKNIFKIN
                     TEVRVLLKHDDNPYVIGIDRGERNLLYIVVVDGKGNIVEQYSLNEIINNFNGIRIKTDY
                     HSLLDKKEKERFEARQNWTSIENIKELKAGYISQVVHKICELVEKYDAVIALEDLNSGF
                     KNSRVKVEKQVYQKFEKMLIDKLNYMVDKKSNPCATGGALKGYQITNKFESFKSMSTQN
                     GFIFYIPAWLTSKIDPSTGFVNLLKTKYTSIADSKKFISSFDRIMYVPEEDLFEFALDY
                     KNFSRTDADYIKKWKLYSYGNRIRIFRNPKKNNVFDWEEVCLTSAYKELFNKYGINYQQ
                     GDIRALLCEQSDKAFYSSFMALMSLMLQMRNSITGRTDVDFLISPVKNSDGIFYDSRNY
                     EAQENAILPKNADANGAYNIARKVLWAIGQFKKAEDEKLDKVKIAISNKEWLEYAQTSV
                     KH"
     CDS             4001..4048
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             4055..4135
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     CDS             4136..4162
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             4169..4186
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(4239..4257)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      4253..4300
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      4337..4792
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4424..4443)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4634..4655
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4818..4922
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4923..4991,4992..5783)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5141..5160)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5954..6542
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6443..6462
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6696..6713
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    6728..6867
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(6793..6812)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6953..6975
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(6969..7160)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(7969..9051)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     9013..9032
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(9052..9129)
                     /gene="lacI"
                     /label=lacI promoter
ORIGIN
        1 ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg
       61 cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg
      121 gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga
      181 aattaatacg actcactata ggggaattgt gagcggataa caattcccct ctagaaataa
      241 ttttgtttaa ctttaagaag gagatataca tatgcccgcc gccaagcgcg tgaagctgga
      301 cggcggtagc ggcggaggca gcaagctgga gaagtttaca aactgctact ccctgtctaa
      361 gaccctgagg ttcaaggcca tccctgtggg caagacccag gagaacatcg acaataagcg
      421 gctgctggtg gaggacgaga agagagccga ggattataag ggcgtgaaga agctgctgga
      481 tcgctactat ctgtctttta tcaacgacgt gctgcacagc atcaagctga agaatctgaa
      541 caattacatc agcctgttcc ggaagaaaac cagaaccgag aaggagaata aggagctgga
      601 gaacctggag atcaatctgc ggaaggagat cgccaaggcc ttcaagggca acgagggcta
      661 caagtccctg tttaagaagg atatcatcga gacaatcctg ccagagttcc tggacgataa
      721 ggacgagatc gccctggtga acagcttcaa tggctttacc acagccttca ccggcttctt
      781 tgataacaga gagaatatgt tttccgagga ggccaagagc acatccatcg ccttcaggtg
      841 tatcaacgag aatctgaccc gctacatctc taatatggac atcttcgaga aggtggacgc
      901 catctttgat aagcacgagg tgcaggagat caaggagaag atcctgaaca gcgactatga
      961 tgtggaggat ttctttgagg gcgagttctt taactttgtg ctgacacagg agggcatcga
     1021 cgtgtataac gccatcatcg gcggcttcgt gaccgagagc ggcgagaaga tcaagggcct
     1081 gaacgagtac atcaacctgt ataatcagaa aaccaagcag aagctgccta agtttaagcc
     1141 actgtataag caggtgctga gcgatcggga gtctctgagc ttctacggcg agggctatac
     1201 atccgatgag gaggtgctgg aggtgtttag aaacaccctg aacaagaaca gcgagatctt
     1261 cagctccatc aagaagctgg agaagctgtt caagaatttt gacgagtact ctagcgccgg
     1321 catctttgtg aagaacggcc ccgccatcag cacaatctcc aaggatatct tcggcgagtg
     1381 gaacgtgatc cgggacaagt ggaatgccga gtatgacgat atccacctga agaagaaggc
     1441 cgtggtgacc gagaagtacg aggacgatcg gagaaagtcc ttcaagaaga tcggctcctt
     1501 ttctctggag cagctgcagg agtacgccga cgccgatctg tctgtggtgg agaagctgaa
     1561 ggagatcatc atccagaagg tggatgagat ctacaaggtg tatggctcct ctgagaagct
     1621 gttcgacgcc gattttgtgc tggagaagag cctgaagaag aacgacgccg tggtggccat
     1681 catgaaggac ctgctggatt ctgtgaagag cttcgagaat tacatcaagg ccttctttgg
     1741 cgagggcaag gagacaaaca gggacgagtc cttctatggc gattttgtgc tggcctacga
     1801 catcctgctg aaggtggacc acatctacga tgccatccgc aattatgtga cccagaagcc
     1861 ctactctaag gataagttca agctgtattt tcagaaccct cagttcatgg gcggctggga
     1921 caaggataag gagacagact atcgggccac catcctgaga tacggctcca agtactatct
     1981 ggccatcatg gataagaagt acgccaagtg cctgcagaag atcgacaagg acgatgtgaa
     2041 cggcaattac gagaagatca actataagct gctgcccggc cctaataaga tgctgccaaa
     2101 ggtgttcttt tctaagaagt ggatggccta ctataacccc agcgaggaca tccagaagat
     2161 ctacaagaat ggcacattca agaagggcga tatgtttaac ctgaatgact gtcacaagct
     2221 gatcgacttc tttaaggata gcatctcccg gtatccaaag tggtccaatg cctacgattt
     2281 caacttttct gagacagaga agtataagga catcgccggc ttttacagag aggtggagga
     2341 gcagggctat aaggtgagct tcgagtctgc cagcaagaag gaggtggata agctggtgga
     2401 ggagggcaag ctgtatatgt tccagatcta taacaaggac ttttccgata agtctcacgg
     2461 cacacccaat ctgcacacca tgtacttcaa gctgctgttt gacgagaaca atcacggaca
     2521 gatcaggctg agcggaggag cagagctgtt catgaggcgc gcctccctga agaaggagga
     2581 gctggtggtg cacccagcca actcccctat cgccaacaag aatccagata atcccaagaa
     2641 aaccacaacc ctgtcctacg acgtgtataa ggataagagg ttttctgagg accagtacga
     2701 gctgcacatc ccaatcgcca tcaataagtg ccccaagaac atcttcaaga tcaatacaga
     2761 ggtgcgcgtg ctgctgaagc acgacgataa cccctatgtg atcggcatcg ataggggcga
     2821 gcgcaatctg ctgtatatcg tggtggtgga cggcaagggc aacatcgtgg agcagtattc
     2881 cctgaacgag atcatcaaca acttcaacgg catcaggatc aagacagatt accactctct
     2941 gctggacaag aaggagaagg agaggttcga ggcccgccag aactggacct ccatcgagaa
     3001 tatcaaggag ctgaaggccg gctatatctc tcaggtggtg cacaagatct gcgagctggt
     3061 ggagaagtac gatgccgtga tcgccctgga ggacctgaac tctggcttta agaatagccg
     3121 cgtgaaggtg gagaagcagg tgtatcagaa gttcgagaag atgctgatcg ataagctgaa
     3181 ctacatggtg gacaagaagt ctaatccttg tgcaacaggc ggcgccctga agggctatca
     3241 gatcaccaat aagttcgaga gctttaagtc catgtctacc cagaacggct tcatctttta
     3301 catccctgcc tggctgacat ccaagatcga tccatctacc ggctttgtga acctgctgaa
     3361 aaccaagtat accagcatcg ccgattccaa gaagttcatc agctcctttg acaggatcat
     3421 gtacgtgccc gaggaggatc tgttcgagtt tgccctggac tataagaact tctctcgcac
     3481 agacgccgat tacatcaaga agtggaagct gtactcctac ggcaaccgga tcagaatctt
     3541 ccggaatcct aagaagaaca acgtgttcga ctgggaggag gtgtgcctga ccagcgccta
     3601 taaggagctg ttcaacaagt acggcatcaa ttatcagcag ggcgatatca gagccctgct
     3661 gtgcgagcag tccgacaagg ccttctactc tagctttatg gccctgatga gcctgatgct
     3721 gcagatgcgg aacagcatca caggccgcac cgacgtggat tttctgatca gccctgtgaa
     3781 gaactccgac ggcatcttct acgatagccg gaactatgag gcccaggaga atgccatcct
     3841 gccaaagaac gccgacgcca atggcgccta taacatcgcc agaaaggtgc tgtgggccat
     3901 cggccagttc aagaaggccg aggacgagaa gctggataag gtgaagatcg ccatctctaa
     3961 caaggagtgg ctggagtacg cccagaccag cgtgaagcac aaaaggccgg cggccacgaa
     4021 aaaggccggc caggcaaaaa agaaaaaggg atcctaccca tacgatgttc cagattacgc
     4081 ttatccctac gacgtgcctg attatgcata cccatatgat gtccccgact atgccccggc
     4141 agctaagagg gtgaaactgg atctcgagca ccaccaccac caccactgag atccggctgc
     4201 taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata
     4261 accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc
     4321 cggattggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     4381 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     4441 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     4501 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     4561 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     4621 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     4681 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     4741 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca
     4801 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     4861 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     4921 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     4981 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     5041 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     5101 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     5161 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     5221 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     5281 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     5341 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     5401 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     5461 tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     5521 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     5581 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     5641 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     5701 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     5761 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     5821 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     5881 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     5941 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     6001 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     6061 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt
     6121 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     6181 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     6241 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     6301 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     6361 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     6421 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     6481 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     6541 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     6601 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     6661 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     6721 aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcaatg
     6781 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta
     6841 tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc
     6901 tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc
     6961 tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc
     7021 tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg
     7081 ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg
     7141 gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta
     7201 atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc
     7261 cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga
     7321 aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt
     7381 agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc
     7441 gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca
     7501 gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa
     7561 ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc
     7621 acccgtgggg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg
     7681 ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg
     7741 ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc
     7801 ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc
     7861 atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga
     7921 gatcccggtg cctaatgagt gagctaactt acattaattg cgttgcgctc actgcccgct
     7981 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     8041 ggcggtttgc gtattgggcg ccagggtggt ttttcttttc accagtgaga cgggcaacag
     8101 ctgattgccc ttcaccgcct ggccctgaga gagttgcagc aagcggtcca cgctggtttg
     8161 ccccagcagg cgaaaatcct gtttgatggt ggttaacggc gggatataac atgagctgtc
     8221 ttcggtatcg tcgtatccca ctaccgagat atccgcacca acgcgcagcc cggactcggt
     8281 aatggcgcgc attgcgccca gcgccatctg atcgttggca accagcatcg cagtgggaac
     8341 gatgccctca ttcagcattt gcatggtttg ttgaaaaccg gacatggcac tccagtcgcc
     8401 ttcccgttcc gctatcggct gaatttgatt gcgagtgaga tatttatgcc agccagccag
     8461 acgcagacgc gccgagacag aacttaatgg gcccgctaac agcgcgattt gctggtgacc
     8521 caatgcgacc agatgctcca cgcccagtcg cgtaccgtct tcatgggaga aaataatact
     8581 gttgatgggt gtctggtcag agacatcaag aaataacgcc ggaacattag tgcaggcagc
     8641 ttccacagca atggcatcct ggtcatccag cggatagtta atgatcagcc cactgacgcg
     8701 ttgcgcgaga agattgtgca ccgccgcttt acaggcttcg acgccgcttc gttctaccat
     8761 cgacaccacc acgctggcac ccagttgatc ggcgcgagat ttaatcgccg cgacaatttg
     8821 cgacggcgcg tgcagggcca gactggaggt ggcaacgcca atcagcaacg actgtttgcc
     8881 cgccagttgt tgtgccacgc ggttgggaat gtaattcagc tccgccatcg ccgcttccac
     8941 tttttcccgc gttttcgcag aaacgtggct ggcctggttc accacgcggg aaacggtctg
     9001 ataagagaca ccggcatact ctgcgacatc gtataacgtt actggtttca cattcaccac
     9061 cctgaattga ctctcttccg ggcgctatca tgccataccg cgaaaggttt tgcgccattc
     9121 gatggtgtcc gggatctcga cgctctccct tatgcgactc ctgcattagg aagcagccca
     9181 gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg
     9241 cgcccaacag tccc
//
