LOCUS       sequence-372766-        9491 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pET21a protein expression vector for 1N/2CxNLS-cMyc-NLP-cMyc 
            AspCas12a in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9491)
  AUTHORS   Luk K, Liu P, Zeng J, Wang Y, Maitland SA, Idrizi F, Ponnienselvan 
            K, Iyer S, Zhu LJ, Luban J, Bauer DE, Wolfe SA
  TITLE     Optimization of Nuclear Localization Signal Composition Improves 
            CRISPR-Cas12a Editing Rates in Human Primary Cells.
  JOURNAL   GEN Biotechnol. 2022 Jun;1(3):271-284. doi: 
            10.1089/genbio.2022.0003. Epub 2022 Jun 14.
  PUBMED    38405215
REFERENCE   2  (bases 1 to 9491)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1089/genbio.2022.0003"; journalName: "GEN Biotechnol"; date: 
            "2022-06"; volume: "1"; issue: "3"; pages: "271-284"
COMMENT     Sequence Label: 1N/2CxNLS-cMyc-NLP-cMyc AspCas12a
FEATURES             Location/Qualifiers
     source          1..9491
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     34..53
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     137..156
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        137..155
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    156..180
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             195..217
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             228..254
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             273..4190
                     /codon_start=1
                     /product="CRISPR-associated protein from Acidaminococcus 
                     sp. BV3L6 (Zetsche et al., 2015)"
                     /label=AsCpf1
                     /translation="TQFEGFTNLYQVSKTLRFELIPQGKTLKHIQEQGFIEEDKARNDH
                     YKELKPIIDRIYKTYADQCLQLVQLDWENLSAAIDSYRKEKTEETRNALIEEQATYRNA
                     IHDYFIGRTDNLTDAINKRHAEIYKGLFKAELFNGKVLKQLGTVTTTEHENALLRSFDK
                     FTTYFSGFYENRKNVFSAEDISTAIPHRIVQDNFPKFKENCHIFTRLITAVPSLREHFE
                     NVKKAIGIFVSTSIEEVFSFPFYNQLLTQTQIDLYNQLLGGISREAGTEKIKGLNEVLN
                     LAIQKNDETAHIIASLPHRFIPLFKQILSDRNTLSFILEEFKSDEEVIQSFCKYKTLLR
                     NENVLETAEALFNELNSIDLTHIFISHKKLETISSALCDHWDTLRNALYERRISELTGK
                     ITKSAKEKVQRSLKHEDINLQEIISAAGKELSEAFKQKTSEILSHAHAALDQPLPTTLK
                     KQEEKEILKSQLDSLLGLYHLLDWFAVDESNEVDPEFSARLTGIKLEMEPSLSFYNKAR
                     NYATKKPYSVEKFKLNFQMPTLASGWDVNKEKNNGAILFVKNGLYYLGIMPKQKGRYKA
                     LSFEPTEKTSEGFDKMYYDYFPDAAKMIPKCSTQLKAVTAHFQTHTTPILLSNNFIEPL
                     EITKEIYDLNNPEKEPKKFQTAYAKKTGDQKGYREALCKWIDFTRDFLSKYTKTTSIDL
                     SSLRPSSQYKDLGEYYAELNPLLYHISFQRIAEKEIMDAVETGKLYLFQIYNKDFAKGH
                     HGKPNLHTLYWTGLFSPENLAKTSIKLNGQAELFYRPKSRMKRMAHRLGEKMLNKKLKD
                     QKTPIPDTLYQELYDYVNHRLSHDLSDEARALLPNVITKEVSHEIIKDRRFTSDKFFFH
                     VPITLNYQAANSPSKFNQRVNAYLKEHPETPIIGIDRGERNLIYITVIDSTGKILEQRS
                     LNTIQQFDYQKKLDNREKERVAARQAWSVVGTIKDLKQGYLSQVIHEIVDLMIHYQAVV
                     VLENLNFGFKSKRTGIAEKAVYQQFEKMLIDKLNCLVLKDYPAEKVGGVLNPYQLTDQF
                     TSFAKMGTQSGFLFYVPAPYTSKIDPLTGFVDPFVWKTIKNHESRKHFLEGFDFLHYDV
                     KTGDFILHFKMNRNLSFQRGLPGFMPAWDIVFEKNETQFDAKGTPFIAGKRIVPVIENH
                     RFTGRYRDLYPANELIALLEEKGIVFRDGSNILPKLLENDDSHAIDTMVALIRSVLQMR
                     NSNAATGEDYINSPVRDLNGVCFDSRFQNPEWPMDADANGAYHIALKGQLLLNHLKESK
                     DLKLQNGISNQDWLAYIQELRN"
     regulatory      3910..3919
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4191..4238
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             4245..4325
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     CDS             4326..4352
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             4359..4376
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(4429..4447)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      4443..4490
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      4527..4982
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4614..4633)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4824..4845
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5008..5112
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5113..5181,5182..5973)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5331..5350)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6144..6732
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6633..6652
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6886..6903
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    6918..7057
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(6983..7002)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7143..7165
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(7159..7350)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(8159..9241)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     9203..9222
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(9242..9319)
                     /gene="lacI"
                     /label=lacI promoter
ORIGIN
        1 gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc
       61 aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat
      121 ctcgatcccg cgaaattaat acgactcact ataggggaat tgtgagcgga taacaattcc
      181 cctctagaaa taattttgtt taactttaag aaggagatat acatatgccc gccgccaagc
      241 gcgtgaagct ggacggcggt agcggcggag gcacacagtt cgagggcttt accaacctgt
      301 atcaggtgag caagacactg cggtttgagc tgatcccaca gggcaagacc ctgaagcaca
      361 tccaggagca gggcttcatc gaggaggaca aggcccgcaa tgatcactac aaggagctga
      421 agcccatcat cgatcggatc tacaagacct atgccgacca gtgcctgcag ctggtgcagc
      481 tggattggga gaacctgagc gccgccatcg actcctatag aaaggagaaa accgaggaga
      541 caaggaacgc cctgatcgag gagcaggcca catatcgcaa tgccatccac gactacttca
      601 tcggccggac agacaacctg accgatgcca tcaataagag acacgccgag atctacaagg
      661 gcctgttcaa ggccgagctg tttaatggca aggtgctgaa gcagctgggc accgtgacca
      721 caaccgagca cgagaacgcc ctgctgcgga gcttcgacaa gtttacaacc tacttctccg
      781 gcttttatga gaacaggaag aacgtgttca gcgccgagga tatcagcaca gccatcccac
      841 accgcatcgt gcaggacaac ttccccaagt ttaaggagaa ttgtcacatc ttcacacgcc
      901 tgatcaccgc cgtgcccagc ctgcgggagc actttgagaa cgtgaagaag gccatcggca
      961 tcttcgtgag cacctccatc gaggaggtgt tttccttccc tttttataac cagctgctga
     1021 cacagaccca gatcgacctg tataaccagc tgctgggagg aatctctcgg gaggcaggca
     1081 ccgagaagat caagggcctg aacgaggtgc tgaatctggc catccagaag aatgatgaga
     1141 cagcccacat catcgcctcc ctgccacaca gattcatccc cctgtttaag cagatcctgt
     1201 ccgataggaa caccctgtct ttcatcctgg aggagtttaa gagcgacgag gaagtgatcc
     1261 agtccttctg caagtacaag acactgctga gaaacgagaa cgtgctggag acagccgagg
     1321 ccctgtttaa cgagctgaac agcatcgacc tgacacacat cttcatcagc cacaagaagc
     1381 tggagacaat cagcagcgcc ctgtgcgacc actgggatac actgaggaat gccctgtatg
     1441 agcggagaat ctccgagctg acaggcaaga tcaccaagtc tgccaaggag aaggtgcagc
     1501 gcagcctgaa gcacgaggat atcaacctgc aggagatcat ctctgccgca ggcaaggagc
     1561 tgagcgaggc cttcaagcag aaaaccagcg agatcctgtc ccacgcacac gccgccctgg
     1621 atcagccact gcctacaacc ctgaagaagc aggaggagaa ggagatcctg aagtctcagc
     1681 tggacagcct gctgggcctg taccacctgc tggactggtt tgccgtggat gagtccaacg
     1741 aggtggaccc cgagttctct gcccggctga ccggcatcaa gctggagatg gagccttctc
     1801 tgagcttcta caacaaggcc agaaattatg ccaccaagaa gccctactcc gtggagaagt
     1861 tcaagctgaa ctttcagatg cctacactgg cctctggctg ggacgtgaat aaggagaaga
     1921 acaatggcgc catcctgttt gtgaagaacg gcctgtacta tctgggcatc atgccaaagc
     1981 agaagggcag gtataaggcc ctgagcttcg agcccacaga gaaaaccagc gagggctttg
     2041 ataagatgta ctatgactac ttccctgatg ccgccaagat gatcccaaag tgcagcaccc
     2101 agctgaaggc cgtgacagcc cactttcaga cccacacaac ccccatcctg ctgtccaaca
     2161 atttcatcga gcctctggag atcacaaagg agatctacga cctgaacaat cctgagaagg
     2221 agccaaagaa gtttcagaca gcctacgcca agaaaaccgg cgaccagaag ggctacagag
     2281 aggccctgtg caagtggatc gacttcacaa gggattttct gtccaagtat accaagacaa
     2341 cctctatcga tctgtctagc ctgcggccat cctctcagta taaggacctg ggcgagtact
     2401 atgccgagct gaatcccctg ctgtaccaca tcagcttcca gagaatcgcc gagaaggaga
     2461 tcatggatgc cgtggagaca ggcaagctgt acctgttcca gatctataac aaggactttg
     2521 ccaagggcca ccacggcaag cctaatctgc acacactgta ttggaccggc ctgttttctc
     2581 cagagaacct ggccaagaca agcatcaagc tgaatggcca ggccgagctg ttctaccgcc
     2641 ctaagtccag gatgaagagg atggcacacc ggctgggaga gaagatgctg aacaagaagc
     2701 tgaaggatca gaaaacccca atccccgaca ccctgtacca ggagctgtac gactatgtga
     2761 atcacagact gtcccacgac ctgtctgatg aggccagggc cctgctgccc aacgtgatca
     2821 ccaaggaggt gtctcacgag atcatcaagg ataggcgctt taccagcgac aagttctttt
     2881 tccacgtgcc tatcacactg aactatcagg ccgccaattc cccatctaag ttcaaccaga
     2941 gggtgaatgc ctacctgaag gagcaccccg agacacctat catcggcatc gatcggggcg
     3001 agagaaacct gatctatatc acagtgatcg actccaccgg caagatcctg gagcagcgga
     3061 gcctgaacac catccagcag tttgattacc agaagaagct ggacaacagg gagaaggaga
     3121 gggtggcagc aaggcaggcc tggtctgtgg tgggcacaat caaggatctg aagcagggct
     3181 atctgagcca ggtcatccac gagatcgtgg acctgatgat ccactaccag gccgtggtgg
     3241 tgctggagaa cctgaatttc ggctttaaga gcaagaggac cggcatcgcc gagaaggccg
     3301 tgtaccagca gttcgagaag atgctgatcg ataagctgaa ttgcctggtg ctgaaggact
     3361 atccagcaga gaaagtggga ggcgtgctga acccatacca gctgacagac cagttcacct
     3421 cctttgccaa gatgggcacc cagtctggct tcctgtttta cgtgcctgcc ccatatacat
     3481 ctaagatcga tcccctgacc ggcttcgtgg accccttcgt gtggaaaacc atcaagaatc
     3541 acgagagccg caagcacttc ctggagggct tcgactttct gcactacgac gtgaaaaccg
     3601 gcgacttcat cctgcacttt aagatgaaca gaaatctgtc cttccagagg ggcctgcccg
     3661 gctttatgcc tgcatgggat atcgtgttcg agaagaacga gacacagttt gacgccaagg
     3721 gcaccccttt catcgccggc aagagaatcg tgccagtgat cgagaatcac agattcaccg
     3781 gcagataccg ggacctgtat cctgccaacg agctgatcgc cctgctggag gagaagggca
     3841 tcgtgttcag ggatggctcc aacatcctgc caaagctgct ggagaatgac gattctcacg
     3901 ccatcgacac catggtggcc ctgatccgca gcgtgctgca gatgcggaac tccaatgccg
     3961 ccacaggcga ggactatatc aacagccccg tgcgcgatct gaatggcgtg tgcttcgact
     4021 cccggtttca gaacccagag tggcccatgg acgccgatgc caatggcgcc taccacatcg
     4081 ccctgaaggg ccagctgctg ctgaatcacc tgaaggagag caaggatctg aagctgcaga
     4141 acggcatctc caatcaggac tggctggcct acatccagga gctgcgcaac aaaaggccgg
     4201 cggccacgaa aaaggccggc caggcaaaaa agaaaaaggg atcctaccca tacgatgttc
     4261 cagattacgc ttatccctac gacgtgcctg attatgcata cccatatgat gtccccgact
     4321 atgccccggc agctaagagg gtgaaactgg atctcgagca ccaccaccac caccactgag
     4381 atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat
     4441 aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag
     4501 gaactatatc cggattggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg
     4561 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     4621 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     4681 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     4741 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     4801 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     4861 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     4921 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat
     4981 ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     5041 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     5101 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     5161 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     5221 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     5281 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     5341 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     5401 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     5461 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     5521 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     5581 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     5641 gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     5701 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     5761 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     5821 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     5881 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     5941 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     6001 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     6061 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     6121 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     6181 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     6241 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg
     6301 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     6361 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     6421 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     6481 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     6541 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     6601 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     6661 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     6721 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     6781 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     6841 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     6901 gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca
     6961 caccgcaatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata
     7021 cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc
     7081 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt
     7141 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct
     7201 gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc
     7261 gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat
     7321 gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt
     7381 catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga
     7441 tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg
     7501 ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt
     7561 tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc
     7621 tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc
     7681 tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc
     7741 attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac
     7801 gatcatgcgc acccgtgggg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg
     7861 tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc
     7921 aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca
     7981 gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc
     8041 gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg
     8101 catcggtcga gatcccggtg cctaatgagt gagctaactt acattaattg cgttgcgctc
     8161 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     8221 cgcggggaga ggcggtttgc gtattgggcg ccagggtggt ttttcttttc accagtgaga
     8281 cgggcaacag ctgattgccc ttcaccgcct ggccctgaga gagttgcagc aagcggtcca
     8341 cgctggtttg ccccagcagg cgaaaatcct gtttgatggt ggttaacggc gggatataac
     8401 atgagctgtc ttcggtatcg tcgtatccca ctaccgagat atccgcacca acgcgcagcc
     8461 cggactcggt aatggcgcgc attgcgccca gcgccatctg atcgttggca accagcatcg
     8521 cagtgggaac gatgccctca ttcagcattt gcatggtttg ttgaaaaccg gacatggcac
     8581 tccagtcgcc ttcccgttcc gctatcggct gaatttgatt gcgagtgaga tatttatgcc
     8641 agccagccag acgcagacgc gccgagacag aacttaatgg gcccgctaac agcgcgattt
     8701 gctggtgacc caatgcgacc agatgctcca cgcccagtcg cgtaccgtct tcatgggaga
     8761 aaataatact gttgatgggt gtctggtcag agacatcaag aaataacgcc ggaacattag
     8821 tgcaggcagc ttccacagca atggcatcct ggtcatccag cggatagtta atgatcagcc
     8881 cactgacgcg ttgcgcgaga agattgtgca ccgccgcttt acaggcttcg acgccgcttc
     8941 gttctaccat cgacaccacc acgctggcac ccagttgatc ggcgcgagat ttaatcgccg
     9001 cgacaatttg cgacggcgcg tgcagggcca gactggaggt ggcaacgcca atcagcaacg
     9061 actgtttgcc cgccagttgt tgtgccacgc ggttgggaat gtaattcagc tccgccatcg
     9121 ccgcttccac tttttcccgc gttttcgcag aaacgtggct ggcctggttc accacgcggg
     9181 aaacggtctg ataagagaca ccggcatact ctgcgacatc gtataacgtt actggtttca
     9241 cattcaccac cctgaattga ctctcttccg ggcgctatca tgccataccg cgaaaggttt
     9301 tgcgccattc gatggtgtcc gggatctcga cgctctccct tatgcgactc ctgcattagg
     9361 aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc
     9421 aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa
     9481 caagcgctca t
//
