LOCUS       sequence-372768-        9491 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pET21a protein expression vector for 3xNLS-NLP-cMyc-cMyc AspCas12a 
            in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9491)
  AUTHORS   Luk K, Liu P, Zeng J, Wang Y, Maitland SA, Idrizi F, Ponnienselvan 
            K, Iyer S, Zhu LJ, Luban J, Bauer DE, Wolfe SA
  TITLE     Optimization of Nuclear Localization Signal Composition Improves 
            CRISPR-Cas12a Editing Rates in Human Primary Cells.
  JOURNAL   GEN Biotechnol. 2022 Jun;1(3):271-284. doi: 
            10.1089/genbio.2022.0003. Epub 2022 Jun 14.
  PUBMED    38405215
REFERENCE   2  (bases 1 to 9491)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1089/genbio.2022.0003"; journalName: "GEN Biotechnol"; date: 
            "2022-06"; volume: "1"; issue: "3"; pages: "271-284"
COMMENT     Sequence Label: 3xNLS-NLP-cMyc-cMyc AspCas12a
FEATURES             Location/Qualifiers
     source          1..9491
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     970..989
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(1009..1086)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     1292..1311
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     1395..1414
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1395..1413
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1414..1438
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             1453..1475
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1483..5403
                     /codon_start=1
                     /product="CRISPR-associated protein from Acidaminococcus 
                     sp. BV3L6 (Zetsche et al., 2015)"
                     /label=AsCpf1
                     /translation="MTQFEGFTNLYQVSKTLRFELIPQGKTLKHIQEQGFIEEDKARND
                     HYKELKPIIDRIYKTYADQCLQLVQLDWENLSAAIDSYRKEKTEETRNALIEEQATYRN
                     AIHDYFIGRTDNLTDAINKRHAEIYKGLFKAELFNGKVLKQLGTVTTTEHENALLRSFD
                     KFTTYFSGFYENRKNVFSAEDISTAIPHRIVQDNFPKFKENCHIFTRLITAVPSLREHF
                     ENVKKAIGIFVSTSIEEVFSFPFYNQLLTQTQIDLYNQLLGGISREAGTEKIKGLNEVL
                     NLAIQKNDETAHIIASLPHRFIPLFKQILSDRNTLSFILEEFKSDEEVIQSFCKYKTLL
                     RNENVLETAEALFNELNSIDLTHIFISHKKLETISSALCDHWDTLRNALYERRISELTG
                     KITKSAKEKVQRSLKHEDINLQEIISAAGKELSEAFKQKTSEILSHAHAALDQPLPTTL
                     KKQEEKEILKSQLDSLLGLYHLLDWFAVDESNEVDPEFSARLTGIKLEMEPSLSFYNKA
                     RNYATKKPYSVEKFKLNFQMPTLASGWDVNKEKNNGAILFVKNGLYYLGIMPKQKGRYK
                     ALSFEPTEKTSEGFDKMYYDYFPDAAKMIPKCSTQLKAVTAHFQTHTTPILLSNNFIEP
                     LEITKEIYDLNNPEKEPKKFQTAYAKKTGDQKGYREALCKWIDFTRDFLSKYTKTTSID
                     LSSLRPSSQYKDLGEYYAELNPLLYHISFQRIAEKEIMDAVETGKLYLFQIYNKDFAKG
                     HHGKPNLHTLYWTGLFSPENLAKTSIKLNGQAELFYRPKSRMKRMAHRLGEKMLNKKLK
                     DQKTPIPDTLYQELYDYVNHRLSHDLSDEARALLPNVITKEVSHEIIKDRRFTSDKFFF
                     HVPITLNYQAANSPSKFNQRVNAYLKEHPETPIIGIDRGERNLIYITVIDSTGKILEQR
                     SLNTIQQFDYQKKLDNREKERVAARQAWSVVGTIKDLKQGYLSQVIHEIVDLMIHYQAV
                     VVLENLNFGFKSKRTGIAEKAVYQQFEKMLIDKLNCLVLKDYPAEKVGGVLNPYQLTDQ
                     FTSFAKMGTQSGFLFYVPAPYTSKIDPLTGFVDPFVWKTIKNHESRKHFLEGFDFLHYD
                     VKTGDFILHFKMNRNLSFQRGLPGFMPAWDIVFEKNETQFDAKGTPFIAGKRIVPVIEN
                     HRFTGRYRDLYPANELIALLEEKGIVFRDGSNILPKLLENDDSHAIDTMVALIRSVLQM
                     RNSNAATGEDYINSPVRDLNGVCFDSRFQNPEWPMDADANGAYHIALKGQLLLNHLKES
                     KDLKLQNGISNQDWLAYIQELRN"
     regulatory      5123..5132
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5404..5451
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             5458..5538
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     CDS             5539..5565
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             5584..5610
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             5617..5634
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5687..5705)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      5701..5748
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      5785..6240
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5872..5891)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6082..6103
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6266..6370
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6371..6439,6440..7231)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6589..6608)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7402..7990
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7891..7910
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8144..8161
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8176..8315
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8241..8260)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8401..8423
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(8417..8608)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(join(9417..9491,1..1008))
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
ORIGIN
        1 ggtttgcgta ttgggcgcca gggtggtttt tcttttcacc agtgagacgg gcaacagctg
       61 attgcccttc accgcctggc cctgagagag ttgcagcaag cggtccacgc tggtttgccc
      121 cagcaggcga aaatcctgtt tgatggtggt taacggcggg atataacatg agctgtcttc
      181 ggtatcgtcg tatcccacta ccgagatatc cgcaccaacg cgcagcccgg actcggtaat
      241 ggcgcgcatt gcgcccagcg ccatctgatc gttggcaacc agcatcgcag tgggaacgat
      301 gccctcattc agcatttgca tggtttgttg aaaaccggac atggcactcc agtcgccttc
      361 ccgttccgct atcggctgaa tttgattgcg agtgagatat ttatgccagc cagccagacg
      421 cagacgcgcc gagacagaac ttaatgggcc cgctaacagc gcgatttgct ggtgacccaa
      481 tgcgaccaga tgctccacgc ccagtcgcgt accgtcttca tgggagaaaa taatactgtt
      541 gatgggtgtc tggtcagaga catcaagaaa taacgccgga acattagtgc aggcagcttc
      601 cacagcaatg gcatcctggt catccagcgg atagttaatg atcagcccac tgacgcgttg
      661 cgcgagaaga ttgtgcaccg ccgctttaca ggcttcgacg ccgcttcgtt ctaccatcga
      721 caccaccacg ctggcaccca gttgatcggc gcgagattta atcgccgcga caatttgcga
      781 cggcgcgtgc agggccagac tggaggtggc aacgccaatc agcaacgact gtttgcccgc
      841 cagttgttgt gccacgcggt tgggaatgta attcagctcc gccatcgccg cttccacttt
      901 ttcccgcgtt ttcgcagaaa cgtggctggc ctggttcacc acgcgggaaa cggtctgata
      961 agagacaccg gcatactctg cgacatcgta taacgttact ggtttcacat tcaccaccct
     1021 gaattgactc tcttccgggc gctatcatgc cataccgcga aaggttttgc gccattcgat
     1081 ggtgtccggg atctcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta
     1141 gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc
     1201 ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga
     1261 gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa
     1321 ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct
     1381 cgatcccgcg aaattaatac gactcactat aggggaattg tgagcggata acaattcccc
     1441 tctagaaata attttgttta actttaagaa ggagatatac atatgacaca gttcgagggc
     1501 tttaccaacc tgtatcaggt gagcaagaca ctgcggtttg agctgatccc acagggcaag
     1561 accctgaagc acatccagga gcagggcttc atcgaggagg acaaggcccg caatgatcac
     1621 tacaaggagc tgaagcccat catcgatcgg atctacaaga cctatgccga ccagtgcctg
     1681 cagctggtgc agctggattg ggagaacctg agcgccgcca tcgactccta tagaaaggag
     1741 aaaaccgagg agacaaggaa cgccctgatc gaggagcagg ccacatatcg caatgccatc
     1801 cacgactact tcatcggccg gacagacaac ctgaccgatg ccatcaataa gagacacgcc
     1861 gagatctaca agggcctgtt caaggccgag ctgtttaatg gcaaggtgct gaagcagctg
     1921 ggcaccgtga ccacaaccga gcacgagaac gccctgctgc ggagcttcga caagtttaca
     1981 acctacttct ccggctttta tgagaacagg aagaacgtgt tcagcgccga ggatatcagc
     2041 acagccatcc cacaccgcat cgtgcaggac aacttcccca agtttaagga gaattgtcac
     2101 atcttcacac gcctgatcac cgccgtgccc agcctgcggg agcactttga gaacgtgaag
     2161 aaggccatcg gcatcttcgt gagcacctcc atcgaggagg tgttttcctt ccctttttat
     2221 aaccagctgc tgacacagac ccagatcgac ctgtataacc agctgctggg aggaatctct
     2281 cgggaggcag gcaccgagaa gatcaagggc ctgaacgagg tgctgaatct ggccatccag
     2341 aagaatgatg agacagccca catcatcgcc tccctgccac acagattcat ccccctgttt
     2401 aagcagatcc tgtccgatag gaacaccctg tctttcatcc tggaggagtt taagagcgac
     2461 gaggaagtga tccagtcctt ctgcaagtac aagacactgc tgagaaacga gaacgtgctg
     2521 gagacagccg aggccctgtt taacgagctg aacagcatcg acctgacaca catcttcatc
     2581 agccacaaga agctggagac aatcagcagc gccctgtgcg accactggga tacactgagg
     2641 aatgccctgt atgagcggag aatctccgag ctgacaggca agatcaccaa gtctgccaag
     2701 gagaaggtgc agcgcagcct gaagcacgag gatatcaacc tgcaggagat catctctgcc
     2761 gcaggcaagg agctgagcga ggccttcaag cagaaaacca gcgagatcct gtcccacgca
     2821 cacgccgccc tggatcagcc actgcctaca accctgaaga agcaggagga gaaggagatc
     2881 ctgaagtctc agctggacag cctgctgggc ctgtaccacc tgctggactg gtttgccgtg
     2941 gatgagtcca acgaggtgga ccccgagttc tctgcccggc tgaccggcat caagctggag
     3001 atggagcctt ctctgagctt ctacaacaag gccagaaatt atgccaccaa gaagccctac
     3061 tccgtggaga agttcaagct gaactttcag atgcctacac tggcctctgg ctgggacgtg
     3121 aataaggaga agaacaatgg cgccatcctg tttgtgaaga acggcctgta ctatctgggc
     3181 atcatgccaa agcagaaggg caggtataag gccctgagct tcgagcccac agagaaaacc
     3241 agcgagggct ttgataagat gtactatgac tacttccctg atgccgccaa gatgatccca
     3301 aagtgcagca cccagctgaa ggccgtgaca gcccactttc agacccacac aacccccatc
     3361 ctgctgtcca acaatttcat cgagcctctg gagatcacaa aggagatcta cgacctgaac
     3421 aatcctgaga aggagccaaa gaagtttcag acagcctacg ccaagaaaac cggcgaccag
     3481 aagggctaca gagaggccct gtgcaagtgg atcgacttca caagggattt tctgtccaag
     3541 tataccaaga caacctctat cgatctgtct agcctgcggc catcctctca gtataaggac
     3601 ctgggcgagt actatgccga gctgaatccc ctgctgtacc acatcagctt ccagagaatc
     3661 gccgagaagg agatcatgga tgccgtggag acaggcaagc tgtacctgtt ccagatctat
     3721 aacaaggact ttgccaaggg ccaccacggc aagcctaatc tgcacacact gtattggacc
     3781 ggcctgtttt ctccagagaa cctggccaag acaagcatca agctgaatgg ccaggccgag
     3841 ctgttctacc gccctaagtc caggatgaag aggatggcac accggctggg agagaagatg
     3901 ctgaacaaga agctgaagga tcagaaaacc ccaatccccg acaccctgta ccaggagctg
     3961 tacgactatg tgaatcacag actgtcccac gacctgtctg atgaggccag ggccctgctg
     4021 cccaacgtga tcaccaagga ggtgtctcac gagatcatca aggataggcg ctttaccagc
     4081 gacaagttct ttttccacgt gcctatcaca ctgaactatc aggccgccaa ttccccatct
     4141 aagttcaacc agagggtgaa tgcctacctg aaggagcacc ccgagacacc tatcatcggc
     4201 atcgatcggg gcgagagaaa cctgatctat atcacagtga tcgactccac cggcaagatc
     4261 ctggagcagc ggagcctgaa caccatccag cagtttgatt accagaagaa gctggacaac
     4321 agggagaagg agagggtggc agcaaggcag gcctggtctg tggtgggcac aatcaaggat
     4381 ctgaagcagg gctatctgag ccaggtcatc cacgagatcg tggacctgat gatccactac
     4441 caggccgtgg tggtgctgga gaacctgaat ttcggcttta agagcaagag gaccggcatc
     4501 gccgagaagg ccgtgtacca gcagttcgag aagatgctga tcgataagct gaattgcctg
     4561 gtgctgaagg actatccagc agagaaagtg ggaggcgtgc tgaacccata ccagctgaca
     4621 gaccagttca cctcctttgc caagatgggc acccagtctg gcttcctgtt ttacgtgcct
     4681 gccccatata catctaagat cgatcccctg accggcttcg tggacccctt cgtgtggaaa
     4741 accatcaaga atcacgagag ccgcaagcac ttcctggagg gcttcgactt tctgcactac
     4801 gacgtgaaaa ccggcgactt catcctgcac tttaagatga acagaaatct gtccttccag
     4861 aggggcctgc ccggctttat gcctgcatgg gatatcgtgt tcgagaagaa cgagacacag
     4921 tttgacgcca agggcacccc tttcatcgcc ggcaagagaa tcgtgccagt gatcgagaat
     4981 cacagattca ccggcagata ccgggacctg tatcctgcca acgagctgat cgccctgctg
     5041 gaggagaagg gcatcgtgtt cagggatggc tccaacatcc tgccaaagct gctggagaat
     5101 gacgattctc acgccatcga caccatggtg gccctgatcc gcagcgtgct gcagatgcgg
     5161 aactccaatg ccgccacagg cgaggactat atcaacagcc ccgtgcgcga tctgaatggc
     5221 gtgtgcttcg actcccggtt tcagaaccca gagtggccca tggacgccga tgccaatggc
     5281 gcctaccaca tcgccctgaa gggccagctg ctgctgaatc acctgaagga gagcaaggat
     5341 ctgaagctgc agaacggcat ctccaatcag gactggctgg cctacatcca ggagctgcgc
     5401 aacaaaaggc cggcggccac gaaaaaggcc ggccaggcaa aaaagaaaaa gggatcctac
     5461 ccatacgatg ttccagatta cgcttatccc tacgacgtgc ctgattatgc atacccatat
     5521 gatgtccccg actatgcccc ggcagctaag agggtgaaac tggatggcgg tagcggcgga
     5581 ggccccgccg ccaagcgcgt gaagctggac ctcgagcacc accaccacca ccactgagat
     5641 ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa
     5701 ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga
     5761 actatatccg gattggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     5821 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     5881 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     5941 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     6001 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     6061 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     6121 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     6181 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt
     6241 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     6301 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     6361 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     6421 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     6481 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     6541 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     6601 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     6661 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     6721 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     6781 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     6841 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     6901 caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     6961 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     7021 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     7081 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     7141 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     7201 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     7261 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     7321 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     7381 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     7441 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     7501 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta
     7561 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     7621 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     7681 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     7741 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     7801 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     7861 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     7921 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     7981 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     8041 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     8101 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     8161 ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca
     8221 ccgcaatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca
     8281 ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg
     8341 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
     8401 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc
     8461 ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt
     8521 ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt
     8581 taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca
     8641 tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg
     8701 aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg
     8761 accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc
     8821 cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg
     8881 acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc
     8941 aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat
     9001 tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga
     9061 tcatgcgcac ccgtggggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt
     9121 tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa
     9181 gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga
     9241 gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga
     9301 cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca
     9361 tcggtcgaga tcccggtgcc taatgagtga gctaacttac attaattgcg ttgcgctcac
     9421 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     9481 cggggagagg c
//
