LOCUS       sequence-372772-        9446 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pET21a protein expression vector for 2xNLS-NLP-cMyc AspCas12a in 
            bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9446)
  AUTHORS   Luk K, Liu P, Zeng J, Wang Y, Maitland SA, Idrizi F, Ponnienselvan 
            K, Iyer S, Zhu LJ, Luban J, Bauer DE, Wolfe SA
  TITLE     Optimization of Nuclear Localization Signal Composition Improves 
            CRISPR-Cas12a Editing Rates in Human Primary Cells.
  JOURNAL   GEN Biotechnol. 2022 Jun;1(3):271-284. doi: 
            10.1089/genbio.2022.0003. Epub 2022 Jun 14.
  PUBMED    38405215
REFERENCE   2  (bases 1 to 9446)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1089/genbio.2022.0003"; journalName: "GEN Biotechnol"; date: 
            "2022-06"; volume: "1"; issue: "3"; pages: "271-284"
COMMENT     Sequence Label: 2xNLS-NLP-cMyc AspCas12a
FEATURES             Location/Qualifiers
     source          1..9446
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(68..1150)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     1112..1131
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(1151..1228)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     1434..1453
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     1537..1556
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1537..1555
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1556..1580
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             1595..1617
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1625..5545
                     /codon_start=1
                     /product="CRISPR-associated protein from Acidaminococcus 
                     sp. BV3L6 (Zetsche et al., 2015)"
                     /label=AsCpf1
                     /translation="MTQFEGFTNLYQVSKTLRFELIPQGKTLKHIQEQGFIEEDKARND
                     HYKELKPIIDRIYKTYADQCLQLVQLDWENLSAAIDSYRKEKTEETRNALIEEQATYRN
                     AIHDYFIGRTDNLTDAINKRHAEIYKGLFKAELFNGKVLKQLGTVTTTEHENALLRSFD
                     KFTTYFSGFYENRKNVFSAEDISTAIPHRIVQDNFPKFKENCHIFTRLITAVPSLREHF
                     ENVKKAIGIFVSTSIEEVFSFPFYNQLLTQTQIDLYNQLLGGISREAGTEKIKGLNEVL
                     NLAIQKNDETAHIIASLPHRFIPLFKQILSDRNTLSFILEEFKSDEEVIQSFCKYKTLL
                     RNENVLETAEALFNELNSIDLTHIFISHKKLETISSALCDHWDTLRNALYERRISELTG
                     KITKSAKEKVQRSLKHEDINLQEIISAAGKELSEAFKQKTSEILSHAHAALDQPLPTTL
                     KKQEEKEILKSQLDSLLGLYHLLDWFAVDESNEVDPEFSARLTGIKLEMEPSLSFYNKA
                     RNYATKKPYSVEKFKLNFQMPTLASGWDVNKEKNNGAILFVKNGLYYLGIMPKQKGRYK
                     ALSFEPTEKTSEGFDKMYYDYFPDAAKMIPKCSTQLKAVTAHFQTHTTPILLSNNFIEP
                     LEITKEIYDLNNPEKEPKKFQTAYAKKTGDQKGYREALCKWIDFTRDFLSKYTKTTSID
                     LSSLRPSSQYKDLGEYYAELNPLLYHISFQRIAEKEIMDAVETGKLYLFQIYNKDFAKG
                     HHGKPNLHTLYWTGLFSPENLAKTSIKLNGQAELFYRPKSRMKRMAHRLGEKMLNKKLK
                     DQKTPIPDTLYQELYDYVNHRLSHDLSDEARALLPNVITKEVSHEIIKDRRFTSDKFFF
                     HVPITLNYQAANSPSKFNQRVNAYLKEHPETPIIGIDRGERNLIYITVIDSTGKILEQR
                     SLNTIQQFDYQKKLDNREKERVAARQAWSVVGTIKDLKQGYLSQVIHEIVDLMIHYQAV
                     VVLENLNFGFKSKRTGIAEKAVYQQFEKMLIDKLNCLVLKDYPAEKVGGVLNPYQLTDQ
                     FTSFAKMGTQSGFLFYVPAPYTSKIDPLTGFVDPFVWKTIKNHESRKHFLEGFDFLHYD
                     VKTGDFILHFKMNRNLSFQRGLPGFMPAWDIVFEKNETQFDAKGTPFIAGKRIVPVIEN
                     HRFTGRYRDLYPANELIALLEEKGIVFRDGSNILPKLLENDDSHAIDTMVALIRSVLQM
                     RNSNAATGEDYINSPVRDLNGVCFDSRFQNPEWPMDADANGAYHIALKGQLLLNHLKES
                     KDLKLQNGISNQDWLAYIQELRN"
     regulatory      5265..5274
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5546..5593
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             5600..5680
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     CDS             5681..5707
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             5714..5731
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5784..5802)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      5798..5845
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      5882..6337
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5969..5988)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6179..6200
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6363..6467
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6468..6536,6537..7328)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6686..6705)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7499..8087
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7988..8007
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8241..8258
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8273..8412
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8338..8357)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8498..8520
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(8514..8705)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 tctcaagggc atcggtcgag atcccggtgc ctaatgagtg agctaactta cattaattgc
       61 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
      121 cggccaacgc gcggggagag gcggtttgcg tattgggcgc cagggtggtt tttcttttca
      181 ccagtgagac gggcaacagc tgattgccct tcaccgcctg gccctgagag agttgcagca
      241 agcggtccac gctggtttgc cccagcaggc gaaaatcctg tttgatggtg gttaacggcg
      301 ggatataaca tgagctgtct tcggtatcgt cgtatcccac taccgagata tccgcaccaa
      361 cgcgcagccc ggactcggta atggcgcgca ttgcgcccag cgccatctga tcgttggcaa
      421 ccagcatcgc agtgggaacg atgccctcat tcagcatttg catggtttgt tgaaaaccgg
      481 acatggcact ccagtcgcct tcccgttccg ctatcggctg aatttgattg cgagtgagat
      541 atttatgcca gccagccaga cgcagacgcg ccgagacaga acttaatggg cccgctaaca
      601 gcgcgatttg ctggtgaccc aatgcgacca gatgctccac gcccagtcgc gtaccgtctt
      661 catgggagaa aataatactg ttgatgggtg tctggtcaga gacatcaaga aataacgccg
      721 gaacattagt gcaggcagct tccacagcaa tggcatcctg gtcatccagc ggatagttaa
      781 tgatcagccc actgacgcgt tgcgcgagaa gattgtgcac cgccgcttta caggcttcga
      841 cgccgcttcg ttctaccatc gacaccacca cgctggcacc cagttgatcg gcgcgagatt
      901 taatcgccgc gacaatttgc gacggcgcgt gcagggccag actggaggtg gcaacgccaa
      961 tcagcaacga ctgtttgccc gccagttgtt gtgccacgcg gttgggaatg taattcagct
     1021 ccgccatcgc cgcttccact ttttcccgcg ttttcgcaga aacgtggctg gcctggttca
     1081 ccacgcggga aacggtctga taagagacac cggcatactc tgcgacatcg tataacgtta
     1141 ctggtttcac attcaccacc ctgaattgac tctcttccgg gcgctatcat gccataccgc
     1201 gaaaggtttt gcgccattcg atggtgtccg ggatctcgac gctctccctt atgcgactcc
     1261 tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat
     1321 ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc
     1381 acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg
     1441 tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt
     1501 ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact ataggggaat
     1561 tgtgagcgga taacaattcc cctctagaaa taattttgtt taactttaag aaggagatat
     1621 acatatgaca cagttcgagg gctttaccaa cctgtatcag gtgagcaaga cactgcggtt
     1681 tgagctgatc ccacagggca agaccctgaa gcacatccag gagcagggct tcatcgagga
     1741 ggacaaggcc cgcaatgatc actacaagga gctgaagccc atcatcgatc ggatctacaa
     1801 gacctatgcc gaccagtgcc tgcagctggt gcagctggat tgggagaacc tgagcgccgc
     1861 catcgactcc tatagaaagg agaaaaccga ggagacaagg aacgccctga tcgaggagca
     1921 ggccacatat cgcaatgcca tccacgacta cttcatcggc cggacagaca acctgaccga
     1981 tgccatcaat aagagacacg ccgagatcta caagggcctg ttcaaggccg agctgtttaa
     2041 tggcaaggtg ctgaagcagc tgggcaccgt gaccacaacc gagcacgaga acgccctgct
     2101 gcggagcttc gacaagttta caacctactt ctccggcttt tatgagaaca ggaagaacgt
     2161 gttcagcgcc gaggatatca gcacagccat cccacaccgc atcgtgcagg acaacttccc
     2221 caagtttaag gagaattgtc acatcttcac acgcctgatc accgccgtgc ccagcctgcg
     2281 ggagcacttt gagaacgtga agaaggccat cggcatcttc gtgagcacct ccatcgagga
     2341 ggtgttttcc ttcccttttt ataaccagct gctgacacag acccagatcg acctgtataa
     2401 ccagctgctg ggaggaatct ctcgggaggc aggcaccgag aagatcaagg gcctgaacga
     2461 ggtgctgaat ctggccatcc agaagaatga tgagacagcc cacatcatcg cctccctgcc
     2521 acacagattc atccccctgt ttaagcagat cctgtccgat aggaacaccc tgtctttcat
     2581 cctggaggag tttaagagcg acgaggaagt gatccagtcc ttctgcaagt acaagacact
     2641 gctgagaaac gagaacgtgc tggagacagc cgaggccctg tttaacgagc tgaacagcat
     2701 cgacctgaca cacatcttca tcagccacaa gaagctggag acaatcagca gcgccctgtg
     2761 cgaccactgg gatacactga ggaatgccct gtatgagcgg agaatctccg agctgacagg
     2821 caagatcacc aagtctgcca aggagaaggt gcagcgcagc ctgaagcacg aggatatcaa
     2881 cctgcaggag atcatctctg ccgcaggcaa ggagctgagc gaggccttca agcagaaaac
     2941 cagcgagatc ctgtcccacg cacacgccgc cctggatcag ccactgccta caaccctgaa
     3001 gaagcaggag gagaaggaga tcctgaagtc tcagctggac agcctgctgg gcctgtacca
     3061 cctgctggac tggtttgccg tggatgagtc caacgaggtg gaccccgagt tctctgcccg
     3121 gctgaccggc atcaagctgg agatggagcc ttctctgagc ttctacaaca aggccagaaa
     3181 ttatgccacc aagaagccct actccgtgga gaagttcaag ctgaactttc agatgcctac
     3241 actggcctct ggctgggacg tgaataagga gaagaacaat ggcgccatcc tgtttgtgaa
     3301 gaacggcctg tactatctgg gcatcatgcc aaagcagaag ggcaggtata aggccctgag
     3361 cttcgagccc acagagaaaa ccagcgaggg ctttgataag atgtactatg actacttccc
     3421 tgatgccgcc aagatgatcc caaagtgcag cacccagctg aaggccgtga cagcccactt
     3481 tcagacccac acaaccccca tcctgctgtc caacaatttc atcgagcctc tggagatcac
     3541 aaaggagatc tacgacctga acaatcctga gaaggagcca aagaagtttc agacagccta
     3601 cgccaagaaa accggcgacc agaagggcta cagagaggcc ctgtgcaagt ggatcgactt
     3661 cacaagggat tttctgtcca agtataccaa gacaacctct atcgatctgt ctagcctgcg
     3721 gccatcctct cagtataagg acctgggcga gtactatgcc gagctgaatc ccctgctgta
     3781 ccacatcagc ttccagagaa tcgccgagaa ggagatcatg gatgccgtgg agacaggcaa
     3841 gctgtacctg ttccagatct ataacaagga ctttgccaag ggccaccacg gcaagcctaa
     3901 tctgcacaca ctgtattgga ccggcctgtt ttctccagag aacctggcca agacaagcat
     3961 caagctgaat ggccaggccg agctgttcta ccgccctaag tccaggatga agaggatggc
     4021 acaccggctg ggagagaaga tgctgaacaa gaagctgaag gatcagaaaa ccccaatccc
     4081 cgacaccctg taccaggagc tgtacgacta tgtgaatcac agactgtccc acgacctgtc
     4141 tgatgaggcc agggccctgc tgcccaacgt gatcaccaag gaggtgtctc acgagatcat
     4201 caaggatagg cgctttacca gcgacaagtt ctttttccac gtgcctatca cactgaacta
     4261 tcaggccgcc aattccccat ctaagttcaa ccagagggtg aatgcctacc tgaaggagca
     4321 ccccgagaca cctatcatcg gcatcgatcg gggcgagaga aacctgatct atatcacagt
     4381 gatcgactcc accggcaaga tcctggagca gcggagcctg aacaccatcc agcagtttga
     4441 ttaccagaag aagctggaca acagggagaa ggagagggtg gcagcaaggc aggcctggtc
     4501 tgtggtgggc acaatcaagg atctgaagca gggctatctg agccaggtca tccacgagat
     4561 cgtggacctg atgatccact accaggccgt ggtggtgctg gagaacctga atttcggctt
     4621 taagagcaag aggaccggca tcgccgagaa ggccgtgtac cagcagttcg agaagatgct
     4681 gatcgataag ctgaattgcc tggtgctgaa ggactatcca gcagagaaag tgggaggcgt
     4741 gctgaaccca taccagctga cagaccagtt cacctccttt gccaagatgg gcacccagtc
     4801 tggcttcctg ttttacgtgc ctgccccata tacatctaag atcgatcccc tgaccggctt
     4861 cgtggacccc ttcgtgtgga aaaccatcaa gaatcacgag agccgcaagc acttcctgga
     4921 gggcttcgac tttctgcact acgacgtgaa aaccggcgac ttcatcctgc actttaagat
     4981 gaacagaaat ctgtccttcc agaggggcct gcccggcttt atgcctgcat gggatatcgt
     5041 gttcgagaag aacgagacac agtttgacgc caagggcacc cctttcatcg ccggcaagag
     5101 aatcgtgcca gtgatcgaga atcacagatt caccggcaga taccgggacc tgtatcctgc
     5161 caacgagctg atcgccctgc tggaggagaa gggcatcgtg ttcagggatg gctccaacat
     5221 cctgccaaag ctgctggaga atgacgattc tcacgccatc gacaccatgg tggccctgat
     5281 ccgcagcgtg ctgcagatgc ggaactccaa tgccgccaca ggcgaggact atatcaacag
     5341 ccccgtgcgc gatctgaatg gcgtgtgctt cgactcccgg tttcagaacc cagagtggcc
     5401 catggacgcc gatgccaatg gcgcctacca catcgccctg aagggccagc tgctgctgaa
     5461 tcacctgaag gagagcaagg atctgaagct gcagaacggc atctccaatc aggactggct
     5521 ggcctacatc caggagctgc gcaacaaaag gccggcggcc acgaaaaagg ccggccaggc
     5581 aaaaaagaaa aagggatcct acccatacga tgttccagat tacgcttatc cctacgacgt
     5641 gcctgattat gcatacccat atgatgtccc cgactatgcc ccggcagcta agagggtgaa
     5701 actggatctc gagcaccacc accaccacca ctgagatccg gctgctaaca aagcccgaaa
     5761 ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc
     5821 taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat tggcgaatgg
     5881 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     5941 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     6001 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     6061 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     6121 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     6181 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     6241 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     6301 aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg
     6361 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     6421 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     6481 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     6541 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     6601 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     6661 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     6721 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     6781 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     6841 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     6901 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     6961 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg
     7021 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     7081 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     7141 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     7201 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     7261 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     7321 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     7381 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     7441 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     7501 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     7561 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     7621 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
     7681 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     7741 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     7801 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     7861 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     7921 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     7981 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     8041 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     8101 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     8161 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     8221 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc
     8281 ggtattttct ccttacgcat ctgtgcggta tttcacaccg caatggtgca ctctcagtac
     8341 aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg
     8401 gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg
     8461 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg
     8521 ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg
     8581 tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga
     8641 agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg
     8701 gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa
     8761 cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt
     8821 tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt
     8881 caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct
     8941 gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac
     9001 gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag
     9061 cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc
     9121 cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggggccgcc
     9181 atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag
     9241 gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg
     9301 ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg
     9361 agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac
     9421 cggaaggagc tgactgggtt gaaggc
//
