LOCUS       sequence-372773-        9491 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pET21a protein expression vector for 3xNLS-NLP-cMyc-cMyc enAspCas12a
            in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9491)
  AUTHORS   Luk K, Liu P, Zeng J, Wang Y, Maitland SA, Idrizi F, Ponnienselvan 
            K, Iyer S, Zhu LJ, Luban J, Bauer DE, Wolfe SA
  TITLE     Optimization of Nuclear Localization Signal Composition Improves 
            CRISPR-Cas12a Editing Rates in Human Primary Cells.
  JOURNAL   GEN Biotechnol. 2022 Jun;1(3):271-284. doi: 
            10.1089/genbio.2022.0003. Epub 2022 Jun 14.
  PUBMED    38405215
REFERENCE   2  (bases 1 to 9491)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1089/genbio.2022.0003"; journalName: "GEN Biotechnol"; date: 
            "2022-06"; volume: "1"; issue: "3"; pages: "271-284"
COMMENT     Sequence Label: 3xNLS-NLP-cMyc-cMyc enAspCas12a
FEATURES             Location/Qualifiers
     source          1..9491
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     711..730
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(750..827)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     1033..1052
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     1136..1155
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1136..1154
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1155..1179
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             1194..1216
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1224..5144
                     /codon_start=1
                     /product="CRISPR-associated protein from Acidaminococcus 
                     sp. BV3L6 (Zetsche et al., 2015)"
                     /label=AsCpf1
                     /translation="MTQFEGFTNLYQVSKTLRFELIPQGKTLKHIQEQGFIEEDKARND
                     HYKELKPIIDRIYKTYADQCLQLVQLDWENLSAAIDSYRKEKTEETRNALIEEQATYRN
                     AIHDYFIGRTDNLTDAINKRHAEIYKGLFKAELFNGKVLKQLGTVTTTEHENALLRSFD
                     KFTTYFSGFYRNRKNVFSAEDISTAIPHRIVQDNFPKFKENCHIFTRLITAVPSLREHF
                     ENVKKAIGIFVSTSIEEVFSFPFYNQLLTQTQIDLYNQLLGGISREAGTEKIKGLNEVL
                     NLAIQKNDETAHIIASLPHRFIPLFKQILSDRNTLSFILEEFKSDEEVIQSFCKYKTLL
                     RNENVLETAEALFNELNSIDLTHIFISHKKLETISSALCDHWDTLRNALYERRISELTG
                     KITKSAKEKVQRSLKHEDINLQEIISAAGKELSEAFKQKTSEILSHAHAALDQPLPTTL
                     KKQEEKEILKSQLDSLLGLYHLLDWFAVDESNEVDPEFSARLTGIKLEMEPSLSFYNKA
                     RNYATKKPYSVEKFKLNFQMPTLARGWDVNREKNNGAILFVKNGLYYLGIMPKQKGRYK
                     ALSFEPTEKTSEGFDKMYYDYFPDAAKMIPKCSTQLKAVTAHFQTHTTPILLSNNFIEP
                     LEITKEIYDLNNPEKEPKKFQTAYAKKTGDQKGYREALCKWIDFTRDFLSKYTKTTSID
                     LSSLRPSSQYKDLGEYYAELNPLLYHISFQRIAEKEIMDAVETGKLYLFQIYNKDFAKG
                     HHGKPNLHTLYWTGLFSPENLAKTSIKLNGQAELFYRPKSRMKRMAHRLGEKMLNKKLK
                     DQKTPIPDTLYQELYDYVNHRLSHDLSDEARALLPNVITKEVSHEIIKDRRFTSDKFFF
                     HVPITLNYQAANSPSKFNQRVNAYLKEHPETPIIGIDRGERNLIYITVIDSTGKILEQR
                     SLNTIQQFDYQKKLDNREKERVAARQAWSVVGTIKDLKQGYLSQVIHEIVDLMIHYQAV
                     VVLENLNFGFKSKRTGIAEKAVYQQFEKMLIDKLNCLVLKDYPAEKVGGVLNPYQLTDQ
                     FTSFAKMGTQSGFLFYVPAPYTSKIDPLTGFVDPFVWKTIKNHESRKHFLEGFDFLHYD
                     VKTGDFILHFKMNRNLSFQRGLPGFMPAWDIVFEKNETQFDAKGTPFIAGKRIVPVIEN
                     HRFTGRYRDLYPANELIALLEEKGIVFRDGSNILPKLLENDDSHAIDTMVALIRSVLQM
                     RNSNAATGEDYINSPVRDLNGVCFDSRFQNPEWPMDADANGAYHIALKGQLLLNHLKES
                     KDLKLQNGISNQDWLAYIQELRN"
     CDS             5145..5192
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             5199..5279
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     CDS             5280..5306
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             5325..5351
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             5358..5375
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5428..5446)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      5442..5489
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      5526..5981
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5613..5632)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5823..5844
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6007..6111
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6112..6180,6181..6972)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6330..6349)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7143..7731
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7632..7651
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7885..7902
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7917..8056
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7982..8001)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8142..8164
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(8158..8349)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(join(9158..9491,1..749))
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
ORIGIN
        1 gccatctgat cgttggcaac cagcatcgca gtgggaacga tgccctcatt cagcatttgc
       61 atggtttgtt gaaaaccgga catggcactc cagtcgcctt cccgttccgc tatcggctga
      121 atttgattgc gagtgagata tttatgccag ccagccagac gcagacgcgc cgagacagaa
      181 cttaatgggc ccgctaacag cgcgatttgc tggtgaccca atgcgaccag atgctccacg
      241 cccagtcgcg taccgtcttc atgggagaaa ataatactgt tgatgggtgt ctggtcagag
      301 acatcaagaa ataacgccgg aacattagtg caggcagctt ccacagcaat ggcatcctgg
      361 tcatccagcg gatagttaat gatcagccca ctgacgcgtt gcgcgagaag attgtgcacc
      421 gccgctttac aggcttcgac gccgcttcgt tctaccatcg acaccaccac gctggcaccc
      481 agttgatcgg cgcgagattt aatcgccgcg acaatttgcg acggcgcgtg cagggccaga
      541 ctggaggtgg caacgccaat cagcaacgac tgtttgcccg ccagttgttg tgccacgcgg
      601 ttgggaatgt aattcagctc cgccatcgcc gcttccactt tttcccgcgt tttcgcagaa
      661 acgtggctgg cctggttcac cacgcgggaa acggtctgat aagagacacc ggcatactct
      721 gcgacatcgt ataacgttac tggtttcaca ttcaccaccc tgaattgact ctcttccggg
      781 cgctatcatg ccataccgcg aaaggttttg cgccattcga tggtgtccgg gatctcgacg
      841 ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag
      901 caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac
      961 ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg
     1021 atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt
     1081 gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaattaata
     1141 cgactcacta taggggaatt gtgagcggat aacaattccc ctctagaaat aattttgttt
     1201 aactttaaga aggagatata catatgacac agttcgaggg ctttaccaac ctgtatcagg
     1261 tgagcaagac actgcggttt gagctgatcc cacagggcaa gaccctgaag cacatccagg
     1321 agcagggctt catcgaggag gacaaggccc gcaatgatca ctacaaggag ctgaagccca
     1381 tcatcgatcg gatctacaag acctatgccg accagtgcct gcagctggtg cagctggatt
     1441 gggagaacct gagcgccgcc atcgactcct atagaaagga gaaaaccgag gagacaagga
     1501 acgccctgat cgaggagcag gccacatatc gcaatgccat ccacgactac ttcatcggcc
     1561 ggacagacaa cctgaccgat gccatcaata agagacacgc cgagatctac aagggcctgt
     1621 tcaaggccga gctgtttaat ggcaaggtgc tgaagcagct gggcaccgtg accacaaccg
     1681 agcacgagaa cgccctgctg cggagcttcg acaagtttac aacctacttc tccggctttt
     1741 atagaaacag gaagaacgtg ttcagcgccg aggatatcag cacagccatc ccacaccgca
     1801 tcgtgcagga caacttcccc aagtttaagg agaattgtca catcttcaca cgcctgatca
     1861 ccgccgtgcc cagcctgcgg gagcactttg agaacgtgaa gaaggccatc ggcatcttcg
     1921 tgagcacctc catcgaggag gtgttttcct tcccttttta taaccagctg ctgacacaga
     1981 cccagatcga cctgtataac cagctgctgg gaggaatctc tcgggaggca ggcaccgaga
     2041 agatcaaggg cctgaacgag gtgctgaatc tggccatcca gaagaatgat gagacagccc
     2101 acatcatcgc ctccctgcca cacagattca tccccctgtt taagcagatc ctgtccgata
     2161 ggaacaccct gtctttcatc ctggaggagt ttaagagcga cgaggaagtg atccagtcct
     2221 tctgcaagta caagacactg ctgagaaacg agaacgtgct ggagacagcc gaggccctgt
     2281 ttaacgagct gaacagcatc gacctgacac acatcttcat cagccacaag aagctggaga
     2341 caatcagcag cgccctgtgc gaccactggg atacactgag gaatgccctg tatgagcgga
     2401 gaatctccga gctgacaggc aagatcacca agtctgccaa ggagaaggtg cagcgcagcc
     2461 tgaagcacga ggatatcaac ctgcaggaga tcatctctgc cgcaggcaag gagctgagcg
     2521 aggccttcaa gcagaaaacc agcgagatcc tgtcccacgc acacgccgcc ctggatcagc
     2581 cactgcctac aaccctgaag aagcaggagg agaaggagat cctgaagtct cagctggaca
     2641 gcctgctggg cctgtaccac ctgctggact ggtttgccgt ggatgagtcc aacgaggtgg
     2701 accccgagtt ctctgcccgg ctgaccggca tcaagctgga gatggagcct tctctgagct
     2761 tctacaacaa ggccagaaat tatgccacca agaagcccta ctccgtggag aagttcaagc
     2821 tgaactttca gatgcctaca ctggccagag gctgggacgt gaatagagag aagaacaatg
     2881 gcgccatcct gtttgtgaag aacggcctgt actatctggg catcatgcca aagcagaagg
     2941 gcaggtataa ggccctgagc ttcgagccca cagagaaaac cagcgagggc tttgataaga
     3001 tgtactatga ctacttccct gatgccgcca agatgatccc aaagtgcagc acccagctga
     3061 aggccgtgac agcccacttt cagacccaca caacccccat cctgctgtcc aacaatttca
     3121 tcgagcctct ggagatcaca aaggagatct acgacctgaa caatcctgag aaggagccaa
     3181 agaagtttca gacagcctac gccaagaaaa ccggcgacca gaagggctac agagaggccc
     3241 tgtgcaagtg gatcgacttc acaagggatt ttctgtccaa gtataccaag acaacctcta
     3301 tcgatctgtc tagcctgcgg ccatcctctc agtataagga cctgggcgag tactatgccg
     3361 agctgaatcc cctgctgtac cacatcagct tccagagaat cgccgagaag gagatcatgg
     3421 atgccgtgga gacaggcaag ctgtacctgt tccagatcta taacaaggac tttgccaagg
     3481 gccaccacgg caagcctaat ctgcacacac tgtattggac cggcctgttt tctccagaga
     3541 acctggccaa gacaagcatc aagctgaatg gccaggccga gctgttctac cgccctaagt
     3601 ccaggatgaa gaggatggca caccggctgg gagagaagat gctgaacaag aagctgaagg
     3661 atcagaaaac cccaatcccc gacaccctgt accaggagct gtacgactat gtgaatcaca
     3721 gactgtccca cgacctgtct gatgaggcca gggccctgct gcccaacgtg atcaccaagg
     3781 aggtgtctca cgagatcatc aaggataggc gctttaccag cgacaagttc tttttccacg
     3841 tgcctatcac actgaactat caggccgcca attccccatc taagttcaac cagagggtga
     3901 atgcctacct gaaggagcac cccgagacac ctatcatcgg catcgatcgg ggcgagagaa
     3961 acctgatcta tatcacagtg atcgactcca ccggcaagat cctggagcag cggagcctga
     4021 acaccatcca gcagtttgat taccagaaga agctggacaa cagggagaag gagagggtgg
     4081 cagcaaggca ggcctggtct gtggtgggca caatcaagga tctgaagcag ggctatctga
     4141 gccaggtcat ccacgagatc gtggacctga tgatccacta ccaggccgtg gtggtgctgg
     4201 agaacctgaa tttcggcttt aagagcaaga ggaccggcat cgccgagaag gccgtgtacc
     4261 agcagttcga gaagatgctg atcgataagc tgaattgcct ggtgctgaag gactatccag
     4321 cagagaaagt gggaggcgtg ctgaacccat accagctgac agaccagttc acctcctttg
     4381 ccaagatggg cacccagtct ggcttcctgt tttacgtgcc tgccccatat acatctaaga
     4441 tcgatcccct gaccggcttc gtggacccct tcgtgtggaa aaccatcaag aatcacgaga
     4501 gccgcaagca cttcctggag ggcttcgact ttctgcacta cgacgtgaaa accggcgact
     4561 tcatcctgca ctttaagatg aacagaaatc tgtccttcca gaggggcctg cccggcttta
     4621 tgcctgcatg ggatatcgtg ttcgagaaga acgagacaca gtttgacgcc aagggcaccc
     4681 ctttcatcgc cggcaagaga atcgtgccag tgatcgagaa tcacagattc accggcagat
     4741 accgggacct gtatcctgcc aacgagctga tcgccctgct ggaggagaag ggcatcgtgt
     4801 tcagggatgg ctccaacatc ctgccaaagc tgctggagaa tgacgattct cacgccatcg
     4861 acacgatggt ggccctgatc cgcagcgtgc tgcagatgcg gaactccaat gccgccacag
     4921 gcgaggacta tatcaacagc cccgtgcgcg atctgaatgg cgtgtgcttc gactcccggt
     4981 ttcagaaccc agagtggcca atggacgccg atgccaatgg cgcctaccac atcgccctga
     5041 agggccagct gctgctgaat cacctgaagg agagcaagga tctgaagctg cagaacggca
     5101 tctccaatca ggactggctg gcctacatcc aggagctgcg caacaaaagg ccggcggcca
     5161 cgaaaaaggc cggccaggca aaaaagaaaa agggatccta cccatacgat gttccagatt
     5221 acgcttatcc ctacgacgtg cctgattatg catacccata tgatgtcccc gactatgccc
     5281 cggcagctaa gagggtgaaa ctggatggcg gtagcggcgg aggccccgcc gccaagcgcg
     5341 tgaagctgga cctcgagcac caccaccacc accactgaga tccggctgct aacaaagccc
     5401 gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg
     5461 cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggattggcga
     5521 atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     5581 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     5641 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     5701 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
     5761 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     5821 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     5881 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     5941 atttaacgcg aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga
     6001 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     6061 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     6121 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     6181 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     6241 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     6301 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     6361 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     6421 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     6481 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     6541 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     6601 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca
     6661 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     6721 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     6781 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     6841 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     6901 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     6961 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     7021 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     7081 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     7141 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     7201 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     7261 ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac
     7321 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     7381 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     7441 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     7501 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     7561 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     7621 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     7681 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     7741 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     7801 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     7861 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg
     7921 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcaatgg tgcactctca
     7981 gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga
     8041 ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg
     8101 tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca
     8161 gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg
     8221 gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc
     8281 cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg
     8341 tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat
     8401 gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga
     8461 acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca
     8521 gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca
     8581 tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact
     8641 ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca
     8701 gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca
     8761 accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggggc
     8821 cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac
     8881 gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt
     8941 cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc
     9001 tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc
     9061 ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgag atcccggtgc
     9121 ctaatgagtg agctaactta cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     9181 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     9241 tattgggcgc cagggtggtt tttcttttca ccagtgagac gggcaacagc tgattgccct
     9301 tcaccgcctg gccctgagag agttgcagca agcggtccac gctggtttgc cccagcaggc
     9361 gaaaatcctg tttgatggtg gttaacggcg ggatataaca tgagctgtct tcggtatcgt
     9421 cgtatcccac taccgagata tccgcaccaa cgcgcagccc ggactcggta atggcgcgca
     9481 ttgcgcccag c
//
