LOCUS       sequence-372777-       12917 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector allowing for EF1aL-driven constitutive expression 
            of E690K mutant human ABCA3:GFP fusion protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12917)
  AUTHORS   Sun YL, Hennessey EE, Heins H, Yang P, Villacorta-Martin C, Kwan J, 
            Gopalan K, James M, Emili A, Cole FS, Wambach JA, Kotton DN
  TITLE     Human pluripotent stem cell modeling of alveolar type 2 cell 
            dysfunction caused by ABCA3 mutations.
  JOURNAL   J Clin Invest. 2024 Jan 16;134(2):e164274. doi: 10.1172/JCI164274.
  PUBMED    38226623
REFERENCE   2  (bases 1 to 12917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Clin 
            Invest."; date: "2024-01-16"; volume: "134(2):e164274. doi"; pages: 
            " 10.1172/JCI164274"
COMMENT     Sequence Label: pHAGE-EF1aL-E690Kmut_ABCA3-GFP
FEATURES             Location/Qualifiers
     source          1..12917
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2223..3401
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3392
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3349..3369
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             8575..9294
                     /codon_start=1
                     /product="Aequorea coerulescens GFP"
                     /label=AcGFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDD
                     GNYKSRAEVKFEGDTLVNRIELTGTDFKEDGNILGNKMEYNYNAHNVYIMTDKAKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYF
                     GFVTAAAITHGMDELYK"
     misc_feature    9307..9895
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9360..9380)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9778..9789)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9970..10203
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(10322..10340)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10408..10512
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10513..10581,10582..11373)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10731..10750)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11544..12132
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12033..12052
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12286..12303
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12543..12564
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12579..12584,12585..12602,12603..12609)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12617..12633
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12622..12644
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12641..12657
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12641..12657
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12707..12726
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccaa ttcgcccttt
     3421 tctctagcag cactgggcgt ccctttcagc aagacgatgg ctgtgctcag gcagctggcg
     3481 ctcctcctct ggaagaacta caccctgcag aagcggaagg tcctggtgac ggtcctggaa
     3541 ctcttcctgc cattgctgtt ttctgggatc ctcatctggc tccgcttgaa gattcagtcg
     3601 gaaaatgtgc ccaacgccac catctacccg ggccagtcca tccaggagct gcctctgttc
     3661 ttcaccttcc ctccgccagg agacacctgg gagcttgcct acatcccttc tcacagtgac
     3721 gctgccaaga ccgtcactga gacagtgcgc agggcacttg tgatcaacat gcgagtgcgc
     3781 ggctttccct ccgagaagga ctttgaggac tacattaggt acgacaactg ctcgtccagc
     3841 gtgctggccg ccgtggtctt cgagcacccc ttcaaccaca gcaaggagcc cctgccgctg
     3901 gcggtgaaat atcacctacg gttcagttac acacggagaa attacatgtg gacccaaaca
     3961 ggctcctttt tcctgaaaga gacagaaggc tggcacacta cttccctttt cccgcttttc
     4021 ccaaacccag gaccaaggga acctacatcc cctgatggcg gagaacctgg gtacatccgg
     4081 gaaggcttcc tggccgtgca gcatgctgtg gaccgggcca tcatggagta ccatgctgat
     4141 gccgccacac gccagctgtt ccagagactg acggtgacca tcaagaggtt cccgtacccg
     4201 ccgttcatcg cagacccctt cctcgtggcc atccagtacc agctgcccct gctgctgctg
     4261 ctcagcttca cctacaccgc gctcaccatt gcccgtgctg tcgtgcagga gaaggaaagg
     4321 aggctgaagg agtacatgcg catgatgggg ctcagcagct ggctgcactg gagtgcctgg
     4381 ttcctcttgt tcttcctctt cctcctcatc gccgcctcct tcatgaccct gctcttctgt
     4441 gtcaaggtga agccaaatgt agccgtgctg tcccgcagcg acccctccct ggtgctcgcc
     4501 ttcctgctgt gcttcgccat ctctaccatc tccttcagct tcatggtcag caccttcttc
     4561 agcaaagcca acatggcagc agccttcgga ggcttcctct acttcttcac ctacatcccc
     4621 tacttcttcg tggcccctcg gtacaactgg atgactctga gccagaagct ctgctcctgc
     4681 ctcctgtcta atgtcgccat ggcaatggga gcccagctca ttgggaaatt tgaggcgaaa
     4741 ggcatgggca tccagtggcg agacctcctg agtcccgtca acgtggacga cgacttctgc
     4801 ttcgggcagg tgctggggat gctgctgctg gactctgtgc tctatggcct ggtgacctgg
     4861 tacatggagg ccgtcttccc agggcagttc ggcgtgcctc agccctggta cttcttcatc
     4921 atgccctcct attggtgtgg gaagccaagg gcggttgcag ggaaggagga agaagacagt
     4981 gaccccgaga aagcactcag aaacgagtac tttgaagccg agccagagga cctggtggcg
     5041 gggatcaaga tcaagcacct gtccaaggtg ttcagggtgg gaaataagga cagggcggcc
     5101 gtcagagacc tgaacctcaa cctgtacgag ggacagatca ccgtcctgct gggccacaac
     5161 ggtgccggga agaccaccac cctctccatg ctcacaggtc tctttccccc gaccagtgga
     5221 cgggcataca tcagcgggta tgaaatttcc caggacatgg ttcagatccg gaagagcctg
     5281 ggcctgtgcc cgcagcacga catcctgttt gacaacttga cagtcgcaga gcacctttat
     5341 ttctacgccc agctgaaggg cctgtcacgt cagaagtgcc ctgaagaagt caagcagatg
     5401 ctgcacatca tcggcctgga ggacaagtgg aactcacgga gccgcttcct gagcgggggc
     5461 atgaggcgca agctctccat cggcatcgcc ctcatcgcag gctccaaggt gctgatactg
     5521 gacaagccca cctcgggcat ggacgccatc tccaggaggg ccatctggga tcttcttcag
     5581 cggcagaaaa gtgaccgcac catcgtgctg accacccact tcatggacga ggctgacctg
     5641 ctgggagacc gcatcgccat catggccaag ggggagctgc agtgctgcgg gtcctcgctg
     5701 ttcctcaagc agaaatacgg tgccggctat cacatgacgc tggtgaagga gccgcactgc
     5761 aacccggaag acatctccca gctggtccac caccacgtgc ccaacgccac gctggagagc
     5821 agcgctgggg ccgagctgtc tttcatcctt cccagagaga gcacgcacag gtttgaaggt
     5881 ctctttgcta aactggagaa gaagcagaaa gagctgggca ttgccagctt tggggcatcc
     5941 atcaccacca tggaggaagt cttccttcgg gtcgggaagc tggtggacag cagtatggac
     6001 atccaggcca tccagctccc tgccctgcag taccagcacg agaggcgcgc cagcgactgg
     6061 gctgtggaca gcaacctctg tggggccatg gacccctccg acggcattgg agccctcatc
     6121 gaggaggagc gcaccgctgt caagctcaac actgggctcg ccctgcactg ccagcaattc
     6181 tgggccatgt tcctgaagaa ggccgcatac agctggcgcg agtggaaaat ggtggcggca
     6241 caggtcctgg tgcctctgac ctgcgtcacc ctggccctcc tggccatcaa ctactcctcg
     6301 gagctcttcg acgaccccat gctgaggctg accttgggcg agtacggcag aaccgtcgtg
     6361 cccttctcag ttcccgggac ctcccagctg ggtcagcagc tgtcagagca tctgaaagac
     6421 gcactgcagg ctgagggaca ggagccccgc gaggtgctcg gtgacctgga ggagttcttg
     6481 atcttcaggg cttctgtgga ggggggcggc tttaatgagc ggtgccttgt ggcagcgtcc
     6541 ttcagagatg tgggagagcg cacggtcgtc aacgccttgt tcaacaacca ggcgtaccac
     6601 tctccagcca ctgccctggc cgtcgtggac aaccttctgt tcaagctgct gtgcgggcct
     6661 cacgcctcca ttgtggtctc caacttcccc cagccccgga gcgccctgca ggctgccaag
     6721 gaccagttta acgagggccg gaagggattc gacattgccc tcaacctgct cttcgccatg
     6781 gcattcttgg ccagcacgtt ctccatcctg gcggtcagcg agagggccgt gcaggccaag
     6841 catgtgcagt ttgtgagtgg agtccacgtg gccagtttct ggctctctgc tctgctgtgg
     6901 gacctcatct ccttcctcat ccccagtctg ctgctgctgg tggtgtttaa ggccttcgac
     6961 gtgcgtgcct tcacgcggga cggccacatg gctgacaccc tgctgctgct cctgctctac
     7021 ggctgggcca tcatccccct catgtacctg atgaacttct tcttcttggg ggcggccact
     7081 gcctacacga ggctgaccat cttcaacatc ctgtcaggca tcgccacctt cctgatggtc
     7141 accatcatgc gcatcccagc tgtaaaactg gaagaacttt ccaaaaccct ggatcacgtg
     7201 ttcctggtgc tgcccaacca ctgtctgggg atggcagtca gcagtttcta cgagaactac
     7261 gagacgcgga ggtactgcac ctcctccgag gtcgccgccc actactgcaa gaaatataac
     7321 atccagtacc aggagaactt ctatgcctgg agcgccccgg gggtcggccg gtttgtggcc
     7381 tccatggccg cctcagggtg cgcctacctc atcctgctct tcctcatcga gaccaacctg
     7441 cttcagagac tcaggggcat cctctgcgcc ctccggagga ggcggacact gacagaatta
     7501 tacacccgga tgcctgtgct tcctgaggac caagatgtag cggacgagag gacccgcatc
     7561 ctggccccca gcccggactc cctgctccac acacctctga ttatcaagga gctctccaag
     7621 gtgtacgagc agcgggtgcc cctcctggcc gtggacaggc tctccctcgc ggtgcagaaa
     7681 ggggagtgct tcggcctgct gggcttcaat ggagccggga agaccacgac tttcaaaatg
     7741 ctgaccgggg aggagagcct cacttctggg gatgcctttg tcgggggtca cagaatcagc
     7801 tctgatgtcg gaaaggtgcg gcagcggatc ggctactgcc cgcagtttga tgccttgctg
     7861 gaccacatga caggccggga gatgctggtc atgtacgctc ggctccgggg catccctgag
     7921 cgccacatcg gggcctgcgt ggagaacact ctgcggggcc tgctgctgga gccacatgcc
     7981 aacaagctgg tcaggacgta cagtggtggt aacaagcgga agctgagcac cggcatcgcc
     8041 ctgatcggag agcctgctgt catcttcctg gacgagccgt ccactggcat ggaccccgtg
     8101 gcccggcgcc tgctttggga caccgtggca cgagcccgag agtctggcaa ggccatcatc
     8161 atcacctccc acagcatgga ggagtgtgag gccctgtgca cccggctggc catcatggtg
     8221 caggggcagt tcaagtgcct gggcagcccc cagcacctca agagcaagtt cggcagcggc
     8281 tactccctgc gggccaaggt gcagagtgaa gggcaacagg aggcgctgga ggagttcaag
     8341 gccttcgtgg acctgacctt tccaggcagc gtcctggaag atgagcacca aggcatggtc
     8401 cattaccacc tgccgggccg tgacctcagc tgggcgaagg ttttcggtat tctggagaaa
     8461 gccaaggaaa agtacggcgt ggacgactac tccgtgagcc agatctcgct ggaacaggtc
     8521 ttcctgagct tcgcccacct gcagccgccc accgcagagg aggggcgacc ggtcatggtg
     8581 agcaagggcg ccgagctgtt caccggcatc gtgcccatcc tgatcgagct gaatggcgat
     8641 gtgaatggcc acaagttcag cgtgagcggc gagggcgagg gcgatgccac ctacggcaag
     8701 ctgaccctga agttcatctg caccaccggc aagctgcctg tgccctggcc caccctggtg
     8761 accaccctga gctacggcgt gcagtgcttc tcacgctacc ccgatcacat gaagcagcac
     8821 gacttcttca agagcgccat gcctgagggc tacatccagg agcgcaccat cttcttcgag
     8881 gatgacggca actacaagtc gcgcgccgag gtgaagttcg agggcgatac cctggtgaat
     8941 cgcatcgagc tgaccggcac cgatttcaag gaggatggca acatcctggg caataagatg
     9001 gagtacaact acaacgccca caatgtgtac atcatgaccg acaaggccaa gaatggcatc
     9061 aaggtgaact tcaagatccg ccacaacatc gaggatggca gcgtgcagct ggccgaccac
     9121 taccagcaga atacccccat cggcgatggc cctgtgctgc tgcccgataa ccactacctg
     9181 tccacccaga gcgccctgtc caaggacccc aacgagaagc gcgatcacat gatctacttc
     9241 ggcttcgtga ccgccgccgc catcacccac ggcatggatg agctgtacaa gtgagcggcc
     9301 gatcctaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     9361 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     9421 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     9481 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     9541 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     9601 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     9661 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg
     9721 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     9781 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     9841 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctgag
     9901 atcctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     9961 aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta
    10021 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
    10081 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
    10141 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
    10201 gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat
    10261 cagagagtga gaggcccggg ttaattaagg aaagggctag atcattcttg aagacgaaag
    10321 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
    10381 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
    10441 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
    10501 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
    10561 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
    10621 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
    10681 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
    10741 gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct
    10801 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
    10861 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
    10921 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
    10981 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
    11041 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
    11101 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
    11161 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
    11221 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
    11281 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
    11341 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
    11401 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
    11461 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
    11521 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
    11581 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
    11641 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
    11701 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
    11761 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
    11821 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
    11881 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
    11941 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
    12001 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
    12061 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
    12121 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
    12181 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
    12241 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
    12301 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
    12361 taatgcagca agctcatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
    12421 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
    12481 aaaagctccc cgtggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
    12541 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
    12601 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgacatg
    12661 attacgaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
    12721 ctcatcaatg tatcttatca tgtctggatc aactggataa ctcaagctaa ccaaaatcat
    12781 cccaaacttc ccaccccata ccctattacc actgccaatt acctgtggtt tcatttactc
    12841 taaacctgtg attcctctga attattttca ttttaaagaa attgtatttg ttaaatatgt
    12901 actacaaact tagtagt
//
