LOCUS       sequence-372778-        9254 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pET21a protein expression vector for 3xNLS-NLP-cMyc-cMyc LbaCas12a 
            in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9254)
  AUTHORS   Luk K, Liu P, Zeng J, Wang Y, Maitland SA, Idrizi F, Ponnienselvan 
            K, Iyer S, Zhu LJ, Luban J, Bauer DE, Wolfe SA
  TITLE     Optimization of Nuclear Localization Signal Composition Improves 
            CRISPR-Cas12a Editing Rates in Human Primary Cells.
  JOURNAL   GEN Biotechnol. 2022 Jun;1(3):271-284. doi: 
            10.1089/genbio.2022.0003. Epub 2022 Jun 14.
  PUBMED    38405215
REFERENCE   2  (bases 1 to 9254)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1089/genbio.2022.0003"; journalName: "GEN Biotechnol"; date: 
            "2022-06"; volume: "1"; issue: "3"; pages: "271-284"
COMMENT     Sequence Label: 3xNLS-NLP-cMyc-cMyc LbaCas12a
FEATURES             Location/Qualifiers
     source          1..9254
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     660..679
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(699..776)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     982..1001
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     1085..1104
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1085..1103
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1104..1128
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             1143..1165
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1173..4856
                     /codon_start=1
                     /product="CRISPR-associated protein from Lachnospiraceae 
                     bacterium ND2006 (Zetsche et al., 2015)"
                     /label=LbCpf1
                     /translation="MSKLEKFTNCYSLSKTLRFKAIPVGKTQENIDNKRLLVEDEKRAE
                     DYKGVKKLLDRYYLSFINDVLHSIKLKNLNNYISLFRKKTRTEKENKELENLEINLRKE
                     IAKAFKGNEGYKSLFKKDIIETILPEFLDDKDEIALVNSFNGFTTAFTGFFDNRENMFS
                     EEAKSTSIAFRCINENLTRYISNMDIFEKVDAIFDKHEVQEIKEKILNSDYDVEDFFEG
                     EFFNFVLTQEGIDVYNAIIGGFVTESGEKIKGLNEYINLYNQKTKQKLPKFKPLYKQVL
                     SDRESLSFYGEGYTSDEEVLEVFRNTLNKNSEIFSSIKKLEKLFKNFDEYSSAGIFVKN
                     GPAISTISKDIFGEWNVIRDKWNAEYDDIHLKKKAVVTEKYEDDRRKSFKKIGSFSLEQ
                     LQEYADADLSVVEKLKEIIIQKVDEIYKVYGSSEKLFDADFVLEKSLKKNDAVVAIMKD
                     LLDSVKSFENYIKAFFGEGKETNRDESFYGDFVLAYDILLKVDHIYDAIRNYVTQKPYS
                     KDKFKLYFQNPQFMGGWDKDKETDYRATILRYGSKYYLAIMDKKYAKCLQKIDKDDVNG
                     NYEKINYKLLPGPNKMLPKVFFSKKWMAYYNPSEDIQKIYKNGTFKKGDMFNLNDCHKL
                     IDFFKDSISRYPKWSNAYDFNFSETEKYKDIAGFYREVEEQGYKVSFESASKKEVDKLV
                     EEGKLYMFQIYNKDFSDKSHGTPNLHTMYFKLLFDENNHGQIRLSGGAELFMRRASLKK
                     EELVVHPANSPIANKNPDNPKKTTTLSYDVYKDKRFSEDQYELHIPIAINKCPKNIFKI
                     NTEVRVLLKHDDNPYVIGIDRGERNLLYIVVVDGKGNIVEQYSLNEIINNFNGIRIKTD
                     YHSLLDKKEKERFEARQNWTSIENIKELKAGYISQVVHKICELVEKYDAVIALEDLNSG
                     FKNSRVKVEKQVYQKFEKMLIDKLNYMVDKKSNPCATGGALKGYQITNKFESFKSMSTQ
                     NGFIFYIPAWLTSKIDPSTGFVNLLKTKYTSIADSKKFISSFDRIMYVPEEDLFEFALD
                     YKNFSRTDADYIKKWKLYSYGNRIRIFRNPKKNNVFDWEEVCLTSAYKELFNKYGINYQ
                     QGDIRALLCEQSDKAFYSSFMALMSLMLQMRNSITGRTDVDFLISPVKNSDGIFYDSRN
                     YEAQENAILPKNADANGAYNIARKVLWAIGQFKKAEDEKLDKVKIAISNKEWLEYAQTS
                     VKH"
     CDS             4857..4904
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             4911..4991
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     CDS             4992..5018
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             5037..5063
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             5070..5087
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5140..5158)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      5154..5201
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      5238..5693
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5325..5344)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5535..5556
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5719..5823
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5824..5892,5893..6684)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPXAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6042..6061)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6855..7443
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7344..7363
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7597..7614
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7629..7768
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7694..7713)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7854..7876
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(7870..8061)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(join(8870..9254,1..698))
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
ORIGIN
        1 agcatttgca tggtttgttg aaaaccggac atggcactcc agtcgccttc ccgttccgct
       61 atcggctgaa tttgattgcg agtgagatat ttatgccagc cagccagacg cagacgcgcc
      121 gagacagaac ttaatgggcc cgctaacagc gcgatttgct ggtgacccaa tgcgaccaga
      181 tgctccacgc ccagtcgcgt accgtcttca tgggagaaaa taatactgtt gatgggtgtc
      241 tggtcagaga catcaagaaa taacgccgga acattagtgc aggcagcttc cacagcaatg
      301 gcatcctggt catccagcgg atagttaatg atcagcccac tgacgcgttg cgcgagaaga
      361 ttgtgcaccg ccgctttaca ggcttcgacg ccgcttcgtt ctaccatcga caccaccacg
      421 ctggcaccca gttgatcggc gcgagattta atcgccgcga caatttgcga cggcgcgtgc
      481 agggccagac tggaggtggc aacgccaatc agcaacgact gtttgcccgc cagttgttgt
      541 gccacgcggt tgggaatgta attcagctcc gccatcgccg cttccacttt ttcccgcgtt
      601 ttcgcagaaa cgtggctggc ctggttcacc acgcgggaaa cggtctgata agagacaccg
      661 gcatactctg cgacatcgta taacgttact ggtttcacat tcaccaccct gaattgactc
      721 tcttccgggc gctatcatgc cataccgcga aaggttttgc gccattcgat ggtgtccggg
      781 atctcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag
      841 gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc
      901 cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg
      961 gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt
     1021 ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg
     1081 aaattaatac gactcactat aggggaattg tgagcggata acaattcccc tctagaaata
     1141 attttgttta actttaagaa ggagatatac atatgagcaa gctggagaag tttacaaact
     1201 gctactccct gtctaagacc ctgaggttca aggccatccc tgtgggcaag acccaggaga
     1261 acatcgacaa taagcggctg ctggtggagg acgagaagag agccgaggat tataagggcg
     1321 tgaagaagct gctggatcgc tactatctgt cttttatcaa cgacgtgctg cacagcatca
     1381 agctgaagaa tctgaacaat tacatcagcc tgttccggaa gaaaaccaga accgagaagg
     1441 agaataagga gctggagaac ctggagatca atctgcggaa ggagatcgcc aaggccttca
     1501 agggcaacga gggctacaag tccctgttta agaaggatat catcgagaca atcctgccag
     1561 agttcctgga cgataaggac gagatcgccc tggtgaacag cttcaatggc tttaccacag
     1621 ccttcaccgg cttctttgat aacagagaga atatgttttc cgaggaggcc aagagcacat
     1681 ccatcgcctt caggtgtatc aacgagaatc tgacccgcta catctctaat atggacatct
     1741 tcgagaaggt ggacgccatc tttgataagc acgaggtgca ggagatcaag gagaagatcc
     1801 tgaacagcga ctatgatgtg gaggatttct ttgagggcga gttctttaac tttgtgctga
     1861 cacaggaggg catcgacgtg tataacgcca tcatcggcgg cttcgtgacc gagagcggcg
     1921 agaagatcaa gggcctgaac gagtacatca acctgtataa tcagaaaacc aagcagaagc
     1981 tgcctaagtt taagccactg tataagcagg tgctgagcga tcgggagtct ctgagcttct
     2041 acggcgaggg ctatacatcc gatgaggagg tgctggaggt gtttagaaac accctgaaca
     2101 agaacagcga gatcttcagc tccatcaaga agctggagaa gctgttcaag aattttgacg
     2161 agtactctag cgccggcatc tttgtgaaga acggccccgc catcagcaca atctccaagg
     2221 atatcttcgg cgagtggaac gtgatccggg acaagtggaa tgccgagtat gacgatatcc
     2281 acctgaagaa gaaggccgtg gtgaccgaga agtacgagga cgatcggaga aagtccttca
     2341 agaagatcgg ctccttttct ctggagcagc tgcaggagta cgccgacgcc gatctgtctg
     2401 tggtggagaa gctgaaggag atcatcatcc agaaggtgga tgagatctac aaggtgtatg
     2461 gctcctctga gaagctgttc gacgccgatt ttgtgctgga gaagagcctg aagaagaacg
     2521 acgccgtggt ggccatcatg aaggacctgc tggattctgt gaagagcttc gagaattaca
     2581 tcaaggcctt ctttggcgag ggcaaggaga caaacaggga cgagtccttc tatggcgatt
     2641 ttgtgctggc ctacgacatc ctgctgaagg tggaccacat ctacgatgcc atccgcaatt
     2701 atgtgaccca gaagccctac tctaaggata agttcaagct gtattttcag aaccctcagt
     2761 tcatgggcgg ctgggacaag gataaggaga cagactatcg ggccaccatc ctgagatacg
     2821 gctccaagta ctatctggcc atcatggata agaagtacgc caagtgcctg cagaagatcg
     2881 acaaggacga tgtgaacggc aattacgaga agatcaacta taagctgctg cccggcccta
     2941 ataagatgct gccaaaggtg ttcttttcta agaagtggat ggcctactat aaccccagcg
     3001 aggacatcca gaagatctac aagaatggca cattcaagaa gggcgatatg tttaacctga
     3061 atgactgtca caagctgatc gacttcttta aggatagcat ctcccggtat ccaaagtggt
     3121 ccaatgccta cgatttcaac ttttctgaga cagagaagta taaggacatc gccggctttt
     3181 acagagaggt ggaggagcag ggctataagg tgagcttcga gtctgccagc aagaaggagg
     3241 tggataagct ggtggaggag ggcaagctgt atatgttcca gatctataac aaggactttt
     3301 ccgataagtc tcacggcaca cccaatctgc acaccatgta cttcaagctg ctgtttgacg
     3361 agaacaatca cggacagatc aggctgagcg gaggagcaga gctgttcatg aggcgcgcct
     3421 ccctgaagaa ggaggagctg gtggtgcacc cagccaactc ccctatcgcc aacaagaatc
     3481 cagataatcc caagaaaacc acaaccctgt cctacgacgt gtataaggat aagaggtttt
     3541 ctgaggacca gtacgagctg cacatcccaa tcgccatcaa taagtgcccc aagaacatct
     3601 tcaagatcaa tacagaggtg cgcgtgctgc tgaagcacga cgataacccc tatgtgatcg
     3661 gcatcgatag gggcgagcgc aatctgctgt atatcgtggt ggtggacggc aagggcaaca
     3721 tcgtggagca gtattccctg aacgagatca tcaacaactt caacggcatc aggatcaaga
     3781 cagattacca ctctctgctg gacaagaagg agaaggagag gttcgaggcc cgccagaact
     3841 ggacctccat cgagaatatc aaggagctga aggccggcta tatctctcag gtggtgcaca
     3901 agatctgcga gctggtggag aagtacgatg ccgtgatcgc cctggaggac ctgaactctg
     3961 gctttaagaa tagccgcgtg aaggtggaga agcaggtgta tcagaagttc gagaagatgc
     4021 tgatcgataa gctgaactac atggtggaca agaagtctaa tccttgtgca acaggcggcg
     4081 ccctgaaggg ctatcagatc accaataagt tcgagagctt taagtccatg tctacccaga
     4141 acggcttcat cttttacatc cctgcctggc tgacatccaa gatcgatcca tctaccggct
     4201 ttgtgaacct gctgaaaacc aagtatacca gcatcgccga ttccaagaag ttcatcagct
     4261 cctttgacag gatcatgtac gtgcccgagg aggatctgtt cgagtttgcc ctggactata
     4321 agaacttctc tcgcacagac gccgattaca tcaagaagtg gaagctgtac tcctacggca
     4381 accggatcag aatcttccgg aatcctaaga agaacaacgt gttcgactgg gaggaggtgt
     4441 gcctgaccag cgcctataag gagctgttca acaagtacgg catcaattat cagcagggcg
     4501 atatcagagc cctgctgtgc gagcagtccg acaaggcctt ctactctagc tttatggccc
     4561 tgatgagcct gatgctgcag atgcggaaca gcatcacagg ccgcaccgac gtggattttc
     4621 tgatcagccc tgtgaagaac tccgacggca tcttctacga tagccggaac tatgaggccc
     4681 aggagaatgc catcctgcca aagaacgccg acgccaatgg cgcctataac atcgccagaa
     4741 aggtgctgtg ggccatcggc cagttcaaga aggccgagga cgagaagctg gataaggtga
     4801 agatcgccat ctctaacaag gagtggctgg agtacgccca gaccagcgtg aagcacaaaa
     4861 ggccggcggc cacgaaaaag gccggccagg caaaaaagaa aaagggatcc tacccatacg
     4921 atgttccaga ttacgcttat ccctacgacg tgcctgatta tgcataccca tatgatgtcc
     4981 ccgactatgc cccggcagct aagagggtga aactggatgg cggtagcggc ggaggccccg
     5041 ccgccaagcg cgtgaagctg gacctcgagc accaccacca ccaccactga gatccggctg
     5101 ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat
     5161 aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat
     5221 ccggattggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     5281 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     5341 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     5401 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
     5461 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     5521 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
     5581 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
     5641 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc
     5701 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     5761 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     5821 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     5881 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     5941 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     6001 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     6061 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     6121 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     6181 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     6241 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     6301 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     6361 atgcctgyag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     6421 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     6481 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     6541 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     6601 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     6661 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     6721 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     6781 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     6841 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     6901 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     6961 aaggtaactg gcttcagcag agcgcagata ccaaatactg tycttctagt gtagccgtag
     7021 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     7081 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     7141 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     7201 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     7261 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     7321 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     7381 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     7441 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     7501 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     7561 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     7621 gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcaat
     7681 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct
     7741 atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc
     7801 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag
     7861 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag
     7921 ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc
     7981 gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc
     8041 ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt
     8101 aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc
     8161 ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag
     8221 aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg
     8281 tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg
     8341 cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc
     8401 agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta
     8461 accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg
     8521 cacccgtggg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc
     8581 gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag
     8641 gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc
     8701 cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt
     8761 catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg
     8821 agatcccggt gcctaatgag tgagctaact tacattaatt gcgttgcgct cactgcccgc
     8881 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     8941 aggcggtttg cgtattgggc gccagggtgg tttttctttt caccagtgag acgggcaaca
     9001 gctgattgcc cttcaccgcc tggccctgag agagttgcag caagcggtcc acgctggttt
     9061 gccccagcag gcgaaaatcc tgtttgatgg tggttaacgg cgggatataa catgagctgt
     9121 cttcggtatc gtcgtatccc actaccgaga tatccgcacc aacgcgcagc ccggactcgg
     9181 taatggcgcg cattgcgccc agcgccatct gatcgttggc aaccagcatc gcagtgggaa
     9241 cgatgccctc attc
//
