LOCUS       sequence-372783-        6837 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Codon optimized blue fluorescent protein mTagBFP2 in C.elegans 
            expresison plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6837)
  AUTHORS   Papadaki S, Wang X, Wang Y, Zhang H, Jia S, Liu S, Yang M, Zhang D, 
            Jia JM, Koster RW, Namikawa K, Piatkevich KD
  TITLE     Dual-expression system for blue fluorescent protein optimization.
  JOURNAL   Sci Rep. 2022 Jun 17;12(1):10190. doi: 10.1038/s41598-022-13214-0.
  PUBMED    35715437
REFERENCE   2  (bases 1 to 6837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2022-06-17"; volume: "12(1):10190. doi"; pages: " 
            10.1038/s41598-022-13214-0"
COMMENT     Sequence Label: pSF11-wmTagBFP2
FEATURES             Location/Qualifiers
     source          1..6837
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(4407..4429)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4529..4548
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4630..4648)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4716..4820
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4821..4889,4890..5681)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5039..5058)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5852..6440
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6341..6360
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6594..6611
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6728..6749
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6764..6769,6770..6787,6788..6794)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6802..6818
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6807..6829
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     join(6826..6837,1..5)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     join(6826..6837,1..5)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 atgaccatga ttacgccaag cttgcatgcg cggccgcact gactgggccg gccggatcca
       61 taggtatgcc tgtcattctc tatttttaaa cgttcctgca gcagtagcac aacagagcca
      121 gcagttttcc tcttcttctc catcttcttc tgcttcttcc tctcttccca aaaaaaaaaa
      181 aaaaaaaaaa gtgagggggc tcactgcccc cccccccctc ccccccccct cttctccttg
      241 aagctcatcc agacgtcgct gtcctcctcc tcctcgtccc cggggcgctg tgtgtgtgtg
      301 tgtgtgctcc gtgagcatgt agcagcttca gcccatttaa aggcgcacac tctctgcatc
      361 tctgcgtgtc tcgacactcg ttttttctct tcttttctgc tgcacacaca cacacacaca
      421 cacacacgct catgccagag cacttcttgc tccggggggc tccgcgcgga gcccgagagg
      481 acctcctcct cctttttctt cttcgctttt tagttcttct tgcctcggtc tcgccccttg
      541 ttttgctctc catgtagtat atacatctgg gtgtgtgtgt gtgtgtgcac ctttggagca
      601 ttttttgaac ctttccagaa ctttggagca acattttcag cttgttcagt gcacttttta
      661 ggctgaaaat cctattttta ggttttcgaa agtttttttg gagcattttt cgtgcaattt
      721 aggtgcaaaa ttaaaaaaaa gggttttgta gtgaattaaa atttttttga aactttcgcc
      781 tttttaaaaa aatgtttcta atatctttaa aaattggaat attttggaaa acaatttgtt
      841 ttcaattggc aatttattaa ttctgagctt ctgagcgtaa attttttttt attttggagc
      901 aattctgaga tgtcttagtg catttttcgg gcatacataa aaaaaaattt atcttaataa
      961 taaaaacctt aaatttaggc ttcaccgtct ggaaattcaa ttttttcagc tgaaaaactt
     1021 tcaaaaaaaa aaaaattaaa ttttcacatt tattttctct ggaattttgg agcaatttca
     1081 aagctgtctt agtgcatttt tagtacacaa atattttttc atttttctta aattttaata
     1141 atctaagctt aaataaaggc ttcaccgtct gaaaatgcaa cttttttcag atgaaatatt
     1201 aaaaaaaaaa cctcttttat taattttgtg ctccaaactt tcccacggta cgcaaatcat
     1261 gtaatatgta cttttaaaca ttatttttcc aatcaaaaat atccaacagc ttttccttac
     1321 aatttgaaaa tttttattaa aatttcaaaa aaattttttt tttttttaat ttcgaatttt
     1381 tttaaacttg ttgtaacttt tttccctgtc ggcgccaatt aacaaaatac gtagaatttt
     1441 gcgcttattg ttttgttagt taacaaattt ttgacaactt tcttatcagc tgagatttag
     1501 gtgtttcagt tcgcaatctg cgtctctcct tgctctcctc tattttaaaa cccccataac
     1561 tttgttcttg tgagagttat caaaaaattg tcaactaaca aaatatagag cacaaaaata
     1621 tgcacatttt gtatgttgac aattttttga tacctttttt agttttcgag atatcttagt
     1681 tttagtttag ctgtaccttt ttctggcaaa aaacttccaa atttcggcaa tttttcggca
     1741 aatttcccgg caaatttcgc caagtacatc gggctctcag acaagtggtc tgtgagtgga
     1801 agtcgataaa agtagtttac gtgctctccg tccccccgct tcttctccgt ctctgcttct
     1861 atttctccat tgactagtcc cactactttt tacggggcgg agggctctct tgcactccag
     1921 cttcttctct ttttgtgctc ttttttgccc gtatgtgggt gctcatttat ttttctttgg
     1981 ttttaaaata aattcggttt attaggctac tcgtaaaaat gaagaaaaaa cttcaaattc
     2041 aaacgacatt aaaaaaaaat ttaataaaaa ttttttgaat tgctcaaaag ccccacctat
     2101 atatatatat agctccgcct acttctcgca cagaccccgc ccactttttg actccgcccc
     2161 cttattggca aaagttcttg aaaaaaataa aaataaaaat tcaaattgaa acaatttttt
     2221 tttaatgaag tagttttttt ctaaattgat gaaaatttct aaacagttta caagcaccgc
     2281 ccactttttg actccgcccc tttgttggaa aaattcttgt aaaaaaaaat aaaaaataaa
     2341 agcttctctt tcaaacgatt tttagttggt ctttgtatga aaagtttttt tcctaaaata
     2401 gaaatgtctg aataatttgc aagctccgcc cttttcttac ccagactccg cccctttatt
     2461 gactaatttt tttttgtcca tttacctgaa gttcaaaaaa aaaatttaca gtttcataga
     2521 gtaataattt ttcaaaaatc tctttttcca atgtttaatg tgtcattata aacccgccca
     2581 gttttaagct ccgcccattt aggccccgcc catttatgac ccaaatattt ttttctctat
     2641 tcacccgtgg atttccctcc aacaagttac cccaaaaata aaaataaaaa ttcctgcagg
     2701 cgcccaccgg gatccggcgc gcctctagag gatccccggg attggccaaa ggacccaaag
     2761 gtatgtttcg aatgatacta acataacata gaacattttc aggaggaccc ttggctagcg
     2821 tcgacggtac caaaaatgtc tgaacttatc aaggaaaaca tgcatatgaa gctttacatg
     2881 gaaggaaccg ttgataacca ccacttcaag tgcacttctg aaggagaggg aaagccatac
     2941 gaaggaactc aaaccatgcg catcaaggtt gttgaaggag gaccacttcc attcgctttc
     3001 gacatccttg ccacctcttt cctttacgga tctaagactt tcatcaacca tactcaagga
     3061 attccagatt tcttcaagca atctttccca gaaggattca cttgggagcg cgttactacc
     3121 tacgaggatg gaggagttct taccgccacc caagatacct cccttcaaga tggatgcctt
     3181 atctacaacg ttaaggtaag tttaaacata tatatactaa ctaaccctga ttatttaaat
     3241 tttcagatcc gcggagttaa cttcacttct aacggaccag ttatgcaaaa gaagactctt
     3301 ggatgggagg ctttcactga gaccctttac ccagccgatg gaggacttga gggacgcaac
     3361 gacatggctc ttaagcttgt tggaggatcc caccttatcg ctaacgctaa gaccacctac
     3421 cgctctaaga agccagccaa gaatcttaag atgccaggag tttattacgt cgattaccgc
     3481 cttgagcgca tcaaggaagc taacaacgag acttacgttg agcaacacga ggttgctgtc
     3541 gctcgttact gcgatcttcc atctaagctt ggacacaagc tcaactaaga gctccgcatc
     3601 ggccgctgtc atcagatcgc catctcgcgc ccgtgcctct gacttctaag tccaattact
     3661 cttcaacatc cctacatgct ctttctccct gtgctcccac cccctatttt tgttattatc
     3721 aaaaaacttc tcttaatttc tttgtttttt agcttctttt aagtcacctc taacaatgaa
     3781 attgtgtaga ttcaaaaata gaattaattc gtaataaaaa gtcgaaaaaa attgtgctcc
     3841 ctccccccat taataataat tctatcccaa aatctacaca atgttctgtg tacacttctt
     3901 atgtttttta cttctgataa atttttttga aacatcatag aaaaaaccgc acacaaaata
     3961 ccttatcata tgttacgttt cagtttatga ccgcaatttt tatttcttcg cacgtctggg
     4021 cctctcatga cgtcaaatca tgctcatcgt gaaaaagttt tggagtattt ttggaatttt
     4081 tcaatcaagt gaaagtttat gaaattaatt ttcctgcttt tgctttttgg ggtttcccct
     4141 attgtttgtc aagatttcga ggacggcgtt tttcttgcta aaatcacaag tattgatgag
     4201 cacgatgcaa gaaagatcgg aagaaggttt gggtttgagg ctcagtggaa ggtgagtaga
     4261 agttgataat ttgaaagtgg agtagtgtct atggggtttt tgccttaaat gacagaatac
     4321 attcccaata taccaaacat aactgtttct actagtcggc cgtacgggcc ctttcgtctc
     4381 gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca
     4441 gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt
     4501 ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac
     4561 catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcggcct
     4621 taattaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     4681 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     4741 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     4801 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     4861 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     4921 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     4981 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     5041 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     5101 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     5161 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     5221 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     5281 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     5341 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     5401 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     5461 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     5521 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     5581 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     5641 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     5701 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     5761 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     5821 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     5881 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     5941 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     6001 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     6061 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     6121 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     6181 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     6241 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     6301 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     6361 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     6421 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     6481 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     6541 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     6601 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     6661 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     6721 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     6781 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagct
//
