LOCUS       sequence-37279-e         669 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tet-regulated (Tet-on) lentiviral vector for transgene (CAG 
            promoter) - OR - shRNA (H1 promoter when subcloned from pLVTHM 
            (Addgene#12247)) - 2nd generation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 669)
  AUTHORS   Szulc J, Wiznerowicz M, Sauvain MO, Trono D, Aebischer P
  TITLE     A versatile tool for conditional gene expression and knockdown.
  JOURNAL   Nat Methods. 2006 Feb;3(2):109-16. doi: 10.1038/nmeth846
  PUBMED    16432520
REFERENCE   2  (bases 1 to 669)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Methods"; date: "2006-02"; volume: "3"; issue: "2"; pages: 
            "109-16"
COMMENT     Sequence Label: pLVCT-tTR-KRAB
FEATURES             Location/Qualifiers
     source          1..669
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      27..36
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    113..230
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     complement(289..308)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     complement(370..392)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
ORIGIN
        1 cccggggaag ctcccgccct tgctcaccat ggtggcgacc ggtagcgcta ggacgcgtaa
       61 gcttaggcct ggatcccgta gtttaaacct cgaggctagt ctcgtgatcg ataaaatttt
      121 gaatttttgt aatttgtttt tgtaattctt tagtttgtat gtctgttgct attatgtcta
      181 ctattctttc ccctgcactg taccccccaa tccccccttt tcttttaaaa gttaaccgat
      241 accgtcgaga ttaattaaat ttaattcttt gccaaaatga tgagacagca caataaccag
      301 cacgttgccc aggagctgta ggaaaaagaa gaaggcatga acatggttag cagaggctct
      361 agagccgccg gtcacacgcc agaagccgaa ccccgccctg ccccgtcccc cccgaaggca
      421 gccgtccccc cgcggacagc cccgaggctg gagagggaga aggggacggc ggcgcggcga
      481 cgcacgaagg ccctccccgc ccatttcctt cctgccggcg ccgcaccgct tcgccccgcg
      541 cccgctagag ggggtgcggc ggcgcctccc agatttcggc tccgcacaga tttgggacaa
      601 aggaagtccc tgcgccctct cgcacgatta ccataaaagg caatggctgc ggctcgccgc
      661 gcctcgaca
//
