LOCUS       sequence-372847-       12370 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express Swedish mutant APP from Ubiquitin C promoter, co-expressing 
            Hygro resistance gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12370)
  TITLE     Hart Lab APP and APOE Plasmids 2022
REFERENCE   2  (bases 1 to 12370)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: FUGW-APPSwe-T2A-Hygro
FEATURES             Location/Qualifiers
     source          1..12370
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1212
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1049..1068
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             complement(1570..1581)
                     /codon_start=1
                     /gene="cspA"
                     /product="translation enhancing element for E. coli (Qing 
                     et al., 2004)"
                     /label=TEE
                     /translation="NHKV"
     CDS             2061..2081
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     CDS             3328..3381
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3388..4413
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             3388..4413
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     misc_feature    4441..5029
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4494..4514)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4912..4923)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5032..5048)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5033..5049)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5554..5734
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5760..5777)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5766..5990
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6036..6464
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6123..6142)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6333..6354
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6473..6493)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6478..6807
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6658..6793
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        6855..6902
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6921..7295
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7425..7546
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7462..7481)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7516..7535
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7595..7611)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7595..7611)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7608..7630)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7619..7635
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7643..7649,7650..7667,7668..7673))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7688..7709
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7826..7843)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7997..8585)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8077..8096)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8756..9547,9548..9616))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9379..9398
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9617..9721)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9796..9815)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        9987..10366
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        10368..10566
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     10521..10541
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             10584..10764
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    10811..10936
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11429..11662
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11847..11891
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    12189..12306
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggcctccgcg ccgggttttg gcgcctcccg cgggcgcccc cctcctcacg gcgagcgctg
       61 ccacgtcaga cgaagggcgc agcgagcgtc ctgatccttc cgcccggacg ctcaggacag
      121 cggcccgctg ctcataagac tcggccttag aaccccagta tcagcagaag gacattttag
      181 gacgggactt gggtgactct agggcactgg ttttctttcc agagagcgga acaggcgagg
      241 aaaagtagtc ccttctcggc gattctgcgg agggatctcc gtggggcggt gaacgccgat
      301 gattatataa ggacgcgccg ggtgtggcac agctagttcc gtcgcagccg ggatttgggt
      361 cgcggttctt gtttgtggat cgctgtgatc gtcacttggt gagtagcggg ctgctgggct
      421 ggccggggct ttcgtggccg ccgggccgct cggtgggacg gaagcgtgtg gagagaccgc
      481 caagggctgt agtctgggtc cgcgagcaag gttgccctga actgggggtt ggggggagcg
      541 cagcaaaatg gcggctgttc ccgagtcttg aatggaagac gcttgtgagg cgggctgtga
      601 ggtcgttgaa acaaggtggg gggcatggtg ggcggcaaga acccaaggtc ttgaggcctt
      661 cgctaatgcg ggaaagctct tattcgggtg agatgggctg gggcaccatc tggggaccct
      721 gacgtgaagt ttgtcactga ctggagaact cggtttgtcg tctgttgcgg gggcggcagt
      781 tatggcggtg ccgttgggca gtgcacccgt acctttggga gcgcgcgccc tcgtcgtgtc
      841 gtgacgtcac ccgttctgtt ggcttataat gcagggtggg gccacctgcc ggtaggtgtg
      901 cggtaggctt ttctccgtcg caggacgcag ggttcgggcc tagggtaggc tctcctgaat
      961 cgacaggcgc cggacctctg gtgaggggag ggataagtga ggcgtcagtt tctttggtcg
     1021 gttttatgta cctatcttct taagtagctg aagctccggt tttgaactat gcgctcgggg
     1081 ttggcgagtg tgttttgtga agttttttag gcaccttttg aaatgtaatc atttgggtca
     1141 atatgtaatt ttcagtgtta gactagtaaa ttgtccgcta aattctggcc gtttttggct
     1201 tttttgttag acgaagcttg ggctgcaggt cgactggcca ccatgctgcc cggtttggca
     1261 ctgctcctgc tggccgcctg gacggctcgg gcgctggagg tacccactga tggtaatgct
     1321 ggcctgctgg ctgaacccca gattgccatg ttctgtggca gactgaacat gcacatgaat
     1381 gtccagaatg ggaagtggga ttcagatcca tcagggacca aaacctgcat tgataccaag
     1441 gaaggcatcc tgcagtattg ccaagaagtc taccctgaac tgcagatcac caatgtggta
     1501 gaagccaacc aaccagtgac catccagaac tggtgcaagc ggggccgcaa gcagtgcaag
     1561 acccatcccc actttgtgat tccctaccgc tgcttagttg gtgagtttgt aagtgatgcc
     1621 cttctcgttc ctgacaagtg caaattctta caccaggaga ggatggatgt ttgcgaaact
     1681 catcttcact ggcacaccgt cgccaaagag acatgcagtg agaagagtac caacttgcat
     1741 gactacggca tgttgctgcc ctgcggaatt gacaagttcc gaggggtaga gtttgtgtgt
     1801 tgcccactgg ctgaagaaag tgacaatgtg gattctgctg atgcggagga ggatgactcg
     1861 gatgtctggt ggggcggagc agacacagac tatgcagatg ggagtgaaga caaagtagta
     1921 gaagtagcag aggaggaaga agtggctgag gtggaagaag aagaagccga tgatgacgag
     1981 gacgatgagg atggtgatga ggtagaggaa gaggctgagg aaccctacga agaagccaca
     2041 gagagaacca ccagcattgc caccaccacc accaccacca cagagtctgt ggaagaggtg
     2101 gttcgagttc ctacaacagc agccagtacc cctgatgccg ttgacaagta tctcgagaca
     2161 cctggggatg agaatgaaca tgcccatttc cagaaagcca aagagaggct tgaggccaag
     2221 caccgagaga gaatgtccca ggtcatgaga gaatgggaag aggcagaacg tcaagcaaag
     2281 aacttgccta aagctgataa gaaggcagtt atccagcatt tccaggagaa agtggaatct
     2341 ttggaacagg aagcagccaa cgagagacag cagctggtgg agacacacat ggccagagtg
     2401 gaagccatgc tcaatgaccg ccgccgcctg gccctggaga actacatcac cgctctgcag
     2461 gctgttcctc ctcggcctcg tcacgtgttc aatatgctaa agaagtatgt ccgcgcagaa
     2521 cagaaggaca gacagcacac cctaaagcat ttcgagcatg tgcgcatggt ggatcccaag
     2581 aaagccgctc agatccggtc ccaggttatg acacacctcc gtgtgattta tgagcgcatg
     2641 aatcagtctc tctccctgct ctacaacgtg cctgcagtgg ccgaggagat tcaggatgaa
     2701 gttgatgagc tgcttcagaa agagcaaaac tattcagatg acgtcttggc caacatgatt
     2761 agtgaaccaa ggatcagtta cggaaacgat gctctcatgc catctttgac cgaaacgaaa
     2821 accaccgtgg agctccttcc cgtgaatgga gagttcagcc tggacgatct ccagccgtgg
     2881 cattcttttg gggctgactc tgtgccagcc aacacagaaa acgaagttga gcctgttgat
     2941 gcccgccctg ctgccgaccg aggactgacc actcgaccag gttctgggtt gacaaatatc
     3001 aagacggagg agatctctga agtgaatctg gatgcagaat tccgacatga ctcaggatat
     3061 gaagttcatc atcaaaaatt ggtgttcttt gcagaagatg tgggttcaaa caaaggtgca
     3121 atcattggac tcatggtggg cggtgttgtc atagcgacag tgatcgtcat caccttggtg
     3181 atgctgaaga agaaacagta cacatccatt catcatggtg tggtggaggt tgacgccgct
     3241 gtcaccccag aggagcgcca cctgtccaag atgcagcaga acggctacga aaatccaacc
     3301 tacaagttct ttgagcagat gcagaacgag ggcaggggaa gtcttctaac atgcggggac
     3361 gtggaggaaa atcccggccc tctagagatg aaaaagcctg aactcaccgc gacgtctgtc
     3421 gagaagtttc tgatcgaaaa gttcgacagc gtctccgacc tgatgcagct ctcggagggc
     3481 gaagaatctc gtgctttcag cttcgatgta ggagggcgtg gatatgtcct gcgggtaaat
     3541 agctgcgccg atggtttcta caaagatcgt tatgtttatc ggcactttgc atcggccgcg
     3601 ctcccgattc cggaagtgct tgacattggg gaattcagcg agagcctgac ctattgcatc
     3661 tcccgccgtg cacagggtgt cacgttgcaa gacctgcctg aaaccgaact gcccgctgtt
     3721 ctgcagccgg tcgcggaggc catggatgcg atcgctgcgg ccgatcttag ccagacgagc
     3781 gggttcggcc cattcggacc gcaaggaatc ggtcaataca ctacatggcg tgatttcata
     3841 tgcgcgattg ctgatcccca tgtgtatcac tggcaaactg tgatggacga caccgtcagt
     3901 gcgtccgtcg cgcaggctct cgatgagctg atgctttggg ccgaggactg ccccgaagtc
     3961 cggcacctcg tgcacgcgga tttcggctcc aacaatgtcc tgacggacaa tggccgcata
     4021 acagcggtca ttgactggag cgaggcgatg ttcggggatt cccaatacga ggtcgccaac
     4081 atcttcttct ggaggccgtg gttggcttgt atggagcagc agacgcgcta cttcgagcgg
     4141 aggcatccgg agcttgcagg atcgccgcgg ctccgggcgt atatgctccg cattggtctt
     4201 gaccaactct atcagagctt ggttgacggc aatttcgatg atgcagcttg ggcgcagggt
     4261 cgatgcgacg caatcgtccg atccggagcc gggactgtcg ggcgtacaca aatcgcccgc
     4321 agaagcgcgg ccgtctggac cgatggctgt gtagaagtac tcgccgatag tggaaaccga
     4381 cgccccagca ctcgtccgag ggcaaaggaa tagctagaat tcgatatcaa gcttatcgat
     4441 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     4501 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     4561 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     4621 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     4681 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     4741 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     4801 ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc
     4861 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     4921 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     4981 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgca tcgataccgt
     5041 cgacctcgag acctagaaaa acatggagca atcacaagta gcaatacagc agctaccaat
     5101 gctgattgtg cctggctaga agcacaagag gaggaggagg tgggttttcc agtcacacct
     5161 caggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
     5221 gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatat ccttgatctg
     5281 tggatctacc acacacaagg ctacttccct gattggcaga actacacacc agggccaggg
     5341 atcagatatc cactgacctt tggatggtgc tacaagctag taccagttga gcaagagaag
     5401 gtagaagaag ccaatgaagg agagaacacc cgcttgttac accctgtgag cctgcatggg
     5461 atggatgacc cggagagaga agtattagag tggaggtttg acagccgcct agcatttcat
     5521 cacatggccc gagagctgca tccggactgt actgggtctc tctggttaga ccagatctga
     5581 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     5641 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     5701 agaccctttt agtcagtgtg gaaaatctct agcagggccc gtttaaaccc gctgatcagc
     5761 ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt
     5821 gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca
     5881 ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga
     5941 ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc
     6001 ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag
     6061 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     6121 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     6181 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     6241 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     6301 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     6361 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
     6421 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg
     6481 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     6541 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
     6601 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     6661 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     6721 atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc
     6781 ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga
     6841 tctgatcagc acgtgttgac aattaatcat cggcatagta tatcggcata gtataatacg
     6901 acaaggtgag gaactaaacc atggccaagt tgaccagtgc cgttccggtg ctcaccgcgc
     6961 gcgacgtcgc cggagcggtc gagttctgga ccgaccggct cgggttctcc cgggacttcg
     7021 tggaggacga cttcgccggt gtggtccggg acgacgtgac cctgttcatc agcgcggtcc
     7081 aggaccaggt ggtgccggac aacaccctgg cctgggtgtg ggtgcgcggc ctggacgagc
     7141 tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg ggacgcctcc gggccggcca
     7201 tgaccgagat cggcgagcag ccgtgggggc gggagttcgc cctgcgcgac ccggccggca
     7261 actgcgtgca cttcgtggcc gaggagcagg actgacacgt gctacgagat ttcgattcca
     7321 ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga
     7381 tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag
     7441 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     7501 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac
     7561 cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     7621 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     7681 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     7741 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     7801 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     7861 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     7921 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     7981 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     8041 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     8101 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     8161 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     8221 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     8281 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     8341 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     8401 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     8461 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     8521 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     8581 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     8641 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     8701 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     8761 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     8821 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     8881 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     8941 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     9001 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     9061 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     9121 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     9181 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     9241 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     9301 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     9361 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     9421 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     9481 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     9541 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     9601 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     9661 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     9721 gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatctc ccgatcccct
     9781 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc
     9841 tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta caacaaggca
     9901 aggcttgacc gacaattgca tgaagaatct gcttagggtt aggcgttttg cgctgcttcg
     9961 cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc
    10021 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
    10081 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
    10141 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
    10201 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
    10261 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
    10321 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
    10381 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
    10441 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
    10501 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
    10561 aagcagcgcg ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc
    10621 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
    10681 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
    10741 agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa
    10801 accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga
    10861 ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga
    10921 tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg
    10981 gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga
    11041 gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat
    11101 actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa
    11161 tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc
    11221 tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc
    11281 tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat
    11341 ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg
    11401 tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag
    11461 caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt
    11521 ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt
    11581 tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat
    11641 acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca
    11701 ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca
    11761 cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa
    11821 ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat
    11881 gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata aaattattca
    11941 taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga
    12001 atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg
    12061 gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca
    12121 ttcgattagt gaacggatcg gcactgcgtg cgccaattct gcagacaaat ggcagtattc
    12181 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
    12241 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
    12301 aattttcggg tttattacag ggacagcaga gatccagttt ggttaattaa cccgtgtcgg
    12361 ctccagatct
//
