LOCUS       sequence-372863-        6060 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Soma and AIS-localized genetically encoded voltage indicator (GEVI) 
            JEDI-2P in AAV production vector for neuron -specific expression 
            using the promoter hSyn.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6060)
  AUTHORS   Liu Z, Lu X, Villette V, Gou Y, Colbert KL, Lai S, Guan S, Land MA, 
            Lee J, Assefa T, Zollinger DR, Korympidou MM, Vlasits AL, Pang MM, 
            Su S, Cai C, Froudarakis E, Zhou N, Patel SS, Smith CL, Ayon A, 
            Bizouard P, Bradley J, Franke K, Clandinin TR, Giovannucci A, Tolias
            AS, Reimer J, Dieudonne S, St-Pierre F
  TITLE     Sustained deep-tissue voltage recording using a fast indicator 
            evolved for two-photon microscopy.
  JOURNAL   Cell. 2022 Aug 16. pii: S0092-8674(22)00916-3. doi: 
            10.1016/j.cell.2022.07.013.
  PUBMED    35985322
REFERENCE   2  (bases 1 to 6060)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2022 
            Aug 16. pii: S0092-8674(22)00916-3. doi: 
            10.1016/j.cell.2022.07.013."
COMMENT     Sequence Label: pAAV-hSyn-JEDI-2P-Kv-WPRE
FEATURES             Location/Qualifiers
     source          1..6060
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      112..700
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     601..620
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   762..891
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        936..1383
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     regulatory      1415..1424
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    2948..3536
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3001..3021)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3419..3430)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3568..4044
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(3767..3786)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   4084..4224
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4084..4213
                     /label=AAV2 ITR
     rep_origin      4299..4754
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4386..4405)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4596..4617
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4771..4790)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4890..4912
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4950..4968)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5036..5140
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5141..5209,5210..6001)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5359..5378)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
       61 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
      121 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
      181 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
      241 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
      301 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
      361 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
      421 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
      481 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
      541 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
      601 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
      661 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
      721 tttacggttc ctggcctttt gctggccttt tgctcacatg tcctgcaggc agctgcgcgc
      781 tcgctcgctc actgaggccg cccgggcgtc gggcgacctt tggtcgcccg gcctcagtga
      841 gcgagcgagc gcgcagagag ggagtggcca actccatcac taggggttcc tgcggccgca
      901 cgcgtgtgtc tagactgcag agggccctgc gtatgagtgc aagtgggttt taggaccagg
      961 atgaggcggg gtgggggtgc ctacctgacg accgaccccg acccactgga caagcaccca
     1021 acccccattc cccaaattgc gcatccccta tcagagaggg ggaggggaaa caggatgcgg
     1081 cgaggcgcgt gcgcactgcc agcttcagca ccgcggacag tgccttcgcc cccgcctggc
     1141 ggcgcgcgcc accgccgcct cagcactgaa ggcgcgctga cgtcactcgc cggtcccccg
     1201 caaactcccc ttcccggcca ccttggtcgc gtccgcgccg ccgccggccc agccggaccg
     1261 caccacgcga ggcgcgagat aggggggcac gggcgcgacc atctgcgctg cggcgccggc
     1321 gactcagcgc tgcctcagtc tgcggtgggc agcggaggag tcgtgtcgtg cctgagagcg
     1381 cagtcgagaa ggtaccggat cttctagagt cgacgccacc atggagacga ctgtgaggta
     1441 tgaacagggg tcagagctca ctaaaacttc gagctctcca acagcagatg agcccacgat
     1501 aaagattgat gatggtcgtg atgagggtaa tgaacaagac agctgttcca ataccattag
     1561 gagaaaaatt tccccgtttg tgatgtcatt tggattcaga gtatttggag ttgtgcttat
     1621 cattgtagac atcatagtgg tgattgtgga tctggccatc agtgagaaga aaagaggcat
     1681 tagagagatt cttgaaggtg tttccctggc tatagcactc ttcttccttg ttgatgttct
     1741 catgagagtg tttgttgaag gcttcaagaa ctatttccgg tccaaactgg ttactttgga
     1801 tgcagtcata gtagtgggca ctctgctaat taatatgacc tactccttct ctgaccttgc
     1861 tgcctttaac agcgagaacg tgtatattac cgcggataaa cagaaaaacg gcattaaagc
     1921 gaactttacc gtgcgccata acgtggaaga tggcagcgtg cagctggcgg atcattatca
     1981 gcagaacacc ccgattggcg atggcccggt gctgctgccg gataaccatt atctgagcca
     2041 ccagaccgtg ctgagcaaag atccgaacga aaaacgcgat cacatggtgc tgctggaatt
     2101 tgtgaccgca gcgggcatta cacacggcat ggatgaactg tatggcggca ccggcggcag
     2161 cgcgagccag ggcgaagaac tgtttaccgg cgtggtgccg attctggtgg aactggatgg
     2221 cgatgtgaac ggccataaat ttagcgtgcg cggcgaaggc gaaggcgatg cgaccattgg
     2281 caaactgacc ctgaaattta tttgcaccac cggcaaacta ccggtgccgt ggccgaccct
     2341 ggtgaccacc ttaacctatg gcgtgcagtg ctttagccgc tatccggatc atatgaaacg
     2401 ccatgatttt tttaaaagcg cgatgccgga aggctatgtg caggaacgca ccattagctt
     2461 taaagatgat ggcaaatata aaacccgcgc ggtggtgaaa tttgaaggcg ataccctggt
     2521 gaaccgcatt gaactgaaag gcaccgattt taaagaagat ggcaacattc tggggcataa
     2581 actggaatat gatacagatc agatgccgca catggttact cttcttcgag ttctgaaaat
     2641 tgttatctta ataagaatat ttcgcctggc ttcacagaag aaacaacttg aagtggtaac
     2701 cggttcttca ggttcatctg gatcttccac gcgtcagtcc cagcccatcc tcaacaccaa
     2761 ggagatggcc ccgcagagca agcctccaga ggagctggag atgagcagca tgcccagccc
     2821 cgtggcccct ctgcccgcac gcacggaggg cgtcatcgac atgcggagca tgtccagcat
     2881 tgacagcttc atcagctgtg ccacggactt ccctgaagcc accagattct gataaaagct
     2941 tatcgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     3001 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     3061 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     3121 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     3181 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     3241 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     3301 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg
     3361 gctgctcgcc tatgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     3421 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     3481 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcatcg
     3541 ataccgagcg ctgctcgaga gatctacggg tggcatccct gtgacccctc cccagtgcct
     3601 ctcctggccc tggaagttgc cactccagtg cccaccagcc ttgtcctaat aaaattaagt
     3661 tgcatcattt tgtctgacta ggtgtccttc tataatatta tggggtggag gggggtggta
     3721 tggagcaagg ggcaagttgg gaagacaacc tgtagggcct gcggggtcta ttgggaacca
     3781 agctggagtg cagtggcaca atcttggctc actgcaatct ccgcctcctg ggttcaagcg
     3841 attctcctgc ctcagcctcc cgagttgttg ggattccagg catgcatgac caggctcagc
     3901 taatttttgt ttttttggta gagacggggt ttcaccatat tggccaggct ggtctccaac
     3961 tcctaatctc aggtgatcta cccaccttgg cctcccaaat tgctgggatt acaggcgtga
     4021 accactgctc ccttccctgt ccttctgatt ttgtaggtaa ccacgtgcgg accgagcggc
     4081 cgcaggaacc cctagtgatg gagttggcca ctccctctct gcgcgctcgc tcgctcactg
     4141 aggccgggcg accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg
     4201 agcgagcgcg cagctgcctg caggggcgcc tgatgcggta ttttctcctt acgcatctgt
     4261 gcggtatttc acaccgcata cgtcaaagca accatagtac gcgccctgta gcggcgcatt
     4321 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccttagc
     4381 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     4441 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     4501 caaaaaactt gatttgggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     4561 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     4621 aacactcaac tctatctcgg gctattcttt tgatttataa gggattttgc cgatttcggt
     4681 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
     4741 aacgtttaca attttatggt gcactctcag tacaatctgc tctgatgccg catagttaag
     4801 ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc
     4861 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc
     4921 gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa
     4981 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
     5041 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     5101 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     5161 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     5221 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     5281 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     5341 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     5401 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     5461 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     5521 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     5581 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     5641 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     5701 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     5761 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     5821 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     5881 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     5941 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     6001 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
//
