LOCUS       sequence-372893-        1029 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of N-terminally mGFP and C-terminally mScarlet labelled 
            wildtype FGFR3 (isoform IIIc).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1029)
  AUTHORS   Hartl I, Brumovska V, Striedner Y, Yasari A, Schutz GJ, Sevcsik E, 
            Tiemann-Boege I
  TITLE     Measurement of FGFR3 signaling at the cell membrane via total 
            internal reflection fluorescence microscopy to compare the 
            activation of FGFR3 mutants.
  JOURNAL   J Biol Chem. 2023 Feb;299(2):102832. doi: 10.1016/j.jbc.2022.102832.
            Epub 2022 Dec 27.
  PUBMED    36581204
REFERENCE   2  (bases 1 to 1029)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2023-02"; volume: "299(2):102832. doi"; pages: " 
            10.1016/j.jbc.2022.102832. Epub 2022 Dec 27"
COMMENT     Sequence Label: pcDNA3.1_mGFP-FGFR3-mScarlet
FEATURES             Location/Qualifiers
     source          1..1029
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(11..706)
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet-I
                     /note="matures faster than mScarlet"
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFIKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     primer_bind     complement(286..305)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     555..573
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 ctctagatta cttgtacagc tcgtccatgc cgccggtgga gtggcggccc tcggagcgtt
       61 cgtactgttc caccacggtg tagtcctcgt tgtgggaggt gatgtccaac ttgcggtcga
      121 cgttgtaggc gccgggcatc tgcacgggct tcttggcctt gtaggtggtc ttgaagtccg
      181 ccaggtagcg gccgccgtcc ttcaggcgca gggccatctt aatgtcgccc ttcagcacgc
      241 cgtcctcggg gtacaaccgc tcggtggacg cttcccagcc cattgtcttc ttctgcatta
      301 cggggccgtc aggagggaag ttggtgccgc ggagcttcac cttgtagatc agggtgccgt
      361 cctccaggga ggtgtcctgg gtcacggtca cggcgccgcc gtcctcgaag ttcatcacgc
      421 gctcccactt gaagccctcg gggaaggact gcttatagta gtcggggatg tcggcggggt
      481 gcttgatgaa ggccctggag ccgtacatga actgagggga caggatgtcc caggagaagg
      541 gcagggggcc acccttggtc accttcagct tggcggtctg ggtgccctcg taggggcggc
      601 cctcgccctc gccctcgatc tcgaactcgt ggccgttcat ggagccctcc atgtgcacct
      661 tgaaccgcat gaactccttg atcactgcct cgcccttgct caccatgctt ccaccggtcg
      721 tccgcgagcc cccactgctg ggtggggccg ggggcagcag gtcgtgggca aacacggagt
      781 cgtcccctga ggagctggag ctgggggtgt cctggccacc cggggagtac tgctcgaaag
      841 gcgccgacag gtccaggtac tcgtcggtgg acgtcacggt aaggacacgg tccaggtcct
      901 ccaccagctg cttgaaggtg ggcctctggg agggcgcggc atgccagcac tcccgcatga
      961 tcatgtacag gtcgtgtgtg cagttggcgg gcttgtccat gcggtggccc tccttcaaca
     1021 gcttgaaaa
//
