LOCUS       sequence-37290-e         877 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Subach OM, Patterson GH, Ting LM, Wang Y, Condeelis JS, Verkhusha VV
  TITLE     A photoswitchable orange-to-far-red fluorescent protein, PSmOrange.
  JOURNAL   Nat Methods. 2011 Jul 31;8(9):771-7. doi: 10.1038/nmeth.1664.
  PUBMED    21804536
REFERENCE   2  (bases 1 to 877)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2011-07-31- 31"; volume: "8"; 
            issue: "9"; pages: "771-7"
COMMENT     Sequence Label: pKeratin-PSmOrange
FEATURES             Location/Qualifiers
     source          1..877
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(130..840)
                     /codon_start=1
                     /product="orange to far-red photoswitchable variant of the 
                     monomeric fluorescent protein mOrange (Subach et al., 
                     2011)"
                     /label=PSmOrange
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEG
                     FQTAKLKVTKGGPLPFAWDILSPLFTYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNY
                     EDGGVVTVTQDSSLQDGEFIYKVKMRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIRMRLKLKDGGHYTSEVKTTYKAKKSVQLPGAYIVGIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(210..233)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     regulatory      837..846
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg
       61 aggtttttta aagcaagtaa aacctctaca aatgtggtat ggctgattat gatctagagt
      121 cgcggccgct tacttgtaca gctcgtccat gccgccggtg gagtggcggc cctcggcgcg
      181 ttcgtactgt tccacgatgg tgtagtcctc gttgtgggag gtgatgtcca gcttgatgcc
      241 gacgatgtag gcgccgggca gctgcacgga cttcttggcc ttgtaggtgg tcttgacctc
      301 ggaggtgtag tggccgccgt ccttcagctt cagcctcatc ctgatctcgc ccttcagggc
      361 gccgtcctcg gggtacatcc gctcggagga ggcctcccag cccatggtct tcttctgcat
      421 cacggggccg tcggagggga agttggtgcc gcgcatcttc accttgtaga tgaactcgcc
      481 gtcctgcagt gaggagtcct gggtcacggt caccacgccg ccgtcctcgt agttcatcac
      541 gcgctcccac ttgaagccct cggggaagga cagcttgaag tagtcgggga tgtcggcggg
      601 gtgcttcacg taggccttgg agccgtaggt gaagagaggg gacaggatgt cccaggcgaa
      661 gggcaggggg ccgcccttgg tcaccttcag cttagcggtc tgaaagccct cgtaggggcg
      721 gccctcgccc tcgccctcga tctcgaactc gtggccgttc acggtgccct ccatgcgcac
      781 cttgaagcgc atgaactcct tgatgatggc catattattc tcctcgccct tgctcaccat
      841 ggtggcgacc ggtggatccc gggcccgcgg taccgtc
//
