LOCUS       sequence-372904-       13202 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian expression vector of Rif1 siRNA resistant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13202)
  AUTHORS   Escribano-Diaz C, Orthwein A, Fradet-Turcotte A, Xing M, Young JT, 
            Tkac J, Cook MA, Rosebrock AP, Munro M, Canny MD, Xu D, Durocher D
  TITLE     A cell cycle-dependent regulatory circuit composed of 53BP1-RIF1 and
            BRCA1-CtIP controls DNA repair pathway choice.
  JOURNAL   Mol Cell. 2013 Mar 7;49(5):872-83. doi: 
            10.1016/j.molcel.2013.01.001. Epub 2013 Jan 17.
  PUBMED    23333306
REFERENCE   2  (bases 1 to 13202)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2013.01.001"; journalName: "Mol Cell"; date: 
            "2013-03-7- 7"; volume: "49"; issue: "5"; pages: "872-83"
COMMENT     Sequence Label: pDEST pcDNA5-FRT/TO-eGFP-Rif1
FEATURES             Location/Qualifiers
     source          1..13202
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     107..126
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    259..483
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     472..489
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     protein_bind    531..555
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7903..7927)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(join(7937..8647,8648..8650,8651..8653))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(7937..8647,8648..8650,8651..8653))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7979..8000)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     8328..8347
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8587..8608
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8750..8774)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    8784..8802
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8805..8823
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        complement(8825..9028)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(8854..8874)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        9029..9408
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     9580..9599
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        9674..9778
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9779..9847,9848..10639)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9997..10016)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10810..11398
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11299..11318
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11552..11569
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11686..11707
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11722..11727,11728..11745,11746..11752)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11760..11776
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11765..11787
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11784..11800
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11784..11800
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    11849..11970
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11860..11879)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     11914..11933
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(12100..13122)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     protein_bind    complement(13130..13177)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
ORIGIN
        1 caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc
       61 gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga
      121 aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac
      181 ccgccgcgct taatgcgccg ctacagggcg cgtggggata ccccctagag ccccagctgg
      241 ttctttccgc ctcagaagcc atagagccca ccgcatcccc agcatgcctg ctattgtctt
      301 cccaatcctc ccccttgctg tcctgcccca ccccaccccc cagaatagaa tgacacctac
      361 tcagacaatg cgatgcaatt tcctcatttt attaggaaag gacagtggga gtggcacctt
      421 ccagggtcaa ggaaggcacg ggggaggggc aaacaacaga tggctggcaa ctagaaggca
      481 cagtcgaggc tgatcagcgg gtttaaacgg gccctctaga ctcgagtcac accactttgt
      541 acaagaaagc tgggtcctaa atagaatttt catgggatgg tgatctccaa cgtgactgta
      601 gattttttat tacagagttt gccatacaac ttagtttctc gtgcatttca aatagttggc
      661 ttcctgaata attatgaaga tcttctgaat ccagctgaag agcaagagca ctaatctgtg
      721 ccacaaggtt tttggataat ggaatttcta aagcaacagg atcaattata tctgagacaa
      781 gtctttcttc atcaggtgac aaagatttat tttctattcc atttactgtt ttttcagaaa
      841 tatcaaaaac tggaatctct tctagtccac gagtcttcac ctgctgctca tgatatattc
      901 tgagagcctt ttttacattg gacacttttg gagaacggat aggaagagtt tttatttcag
      961 atgctgtaag agtactcaaa tcaccaatag tttttatatt cttagctcta atgagttgtc
     1021 ccaggcctct tgcccacatg tttgatgtaa tctgaggtaa aatgatgtca actggtgcca
     1081 cacagttcac caatggtggg taaacacttt ctgttgggca tggtatggat tctttggcca
     1141 tttctgaaat cttagattgt gtagtaggag aagttttaac actttttcct atgggagaac
     1201 tattggaaga atgggaccgt actatagagc accgtctatc aatgtcatct gccaatcctg
     1261 cttggtatat tggatctgca aaggagacac ggcgaacctt attaacaggt gatgagattt
     1321 catcttcttg ggatcttttt agtcctctct ttaaaatgct cgtagacgga gaagccaaag
     1381 gagaccagac acagcgtgtc tgcatgccac taggactgtc atttgctgac acaagtgaag
     1441 gatcaagttc ccttagtttt tgaggagatg cattattgtc ttctattatt agctcagaga
     1501 tagctgttcc agatgccaga cactgtgatg gttcctccac tttctgtgaa gtatggtgat
     1561 cttcctgtaa aatatcactt tccattacca tttgattgtt atctaatttt tcttcagatt
     1621 tactatactc agtatttgtt tcagttttat tagcgtcaat gattccttct tcagtgctca
     1681 tttcaactgt gtcacaaaca aagttgtcca tttctgcagt caacatgttt gtttcagctt
     1741 catcaggctt tgaggttttg gtagttatgc cctcattctc tgtttcacca tcatggccag
     1801 tttcagccat tgcctcgccg atcatcattt cttcataatt tttcattttg gtattcgttt
     1861 cttcagatga gtgtagatca gatacatcat ttcctccatc tagttcccca gctgctgttt
     1921 gttcagaaat ctcagtttga gcattcaatt ttacaggtgt agtattatca gaaagactaa
     1981 tatctgaatt aaattcctca gttgctactt ctttggcatt cagtttagct gtgtctgctt
     2041 cagagtcatc atttctttta ttttcttcta ttgctgcttc ttcagaaata ccagttttgg
     2101 ttctctcttg tccatgaaag ctagcttcat ttcctacatt caattccata gtttttgctt
     2161 tctctagatt ggattctgtt actttacatg catttccttc aacagtaaca ttctctagac
     2221 tcagttccat ttttggggtt tcttctggtt tttcttcttt tgtctccaaa gattcctgtg
     2281 aaacattttt cgagtctcct aaacacgggc caacagtttt aaaattttca ttaggtgact
     2341 cctggctttc aagagacatt gcttcactag aatccatatc acaaatttcc tctctaaagt
     2401 ttactattcc agaaggaaga gattcttgca tagtgttttc ttttgatttg gtttcagaaa
     2461 caattaatga atcattaata gtatcattat cctctttgag acccaaatga aaattcacac
     2521 tatgatgttc atctaaaaat tgtccttcag aatatggatt agcttgagaa gtttctgatg
     2581 ggcttacagg tgcttgcaca tctgcctttt ttgtttcact aatctctacg tcattatttt
     2641 ctgtattctt taacccaatg agtgactttt cctgaggttg tgatttttcc ccacagcaat
     2701 cacaaccttt agatctcctc accctcctac ttctcttgtg ttggcattca agtgtttgaa
     2761 aagttttttc tgaaagcgaa gaaatatctg atatccctat ttttgaggat tctcccaaac
     2821 tacaattatc aaaagagtct cgcttatgta atctcttaat ggcatttcta gtccttagat
     2881 ttgattctgg cacaggtaga cttgtaaagg aatattctgc atatttgcta gtttcatttt
     2941 tttcctcaca cacatttggt aaatcatcag gaacttgcaa caaatctgaa gttactgtag
     3001 aatcctgaca tattacagta ttactttcat cttgtttttc gcaaatcgga gattttatgc
     3061 ttacatcttc ttcagaagtt gctttataat catgtgactg attttcagct tttatacatt
     3121 cctgtttcac cactttctct tcttgatctt gaatgtcaac actttcattg cttttgtttt
     3181 tccattttcc agatctcttc ttcctagaac cttcttcttt tgcctccgaa ctatcagatt
     3241 ctgagttttc aatgctggaa agcaaaccct gagatgctct tctagtttga taccgtgttc
     3301 ggcctctgac tagtttctca gaggacttat ctacaatgtc ctgggtacca tccccctcag
     3361 acttaatgtt ctcagggtct gccttttttt tattttgttc agtttctgca ttagcactag
     3421 tttccccaag agtcttctca tgtaaattac ttcctttctc aattaaattc tcctgcagag
     3481 tttgttcagc aatcagtttt tgtttaggta acacatcatc ttttatatgc aatggactct
     3541 tctgaagagg tttttcttct tccttacgtc gttccttttt ttgatgacta ttttccttat
     3601 cttggctttc agtggtagac tctactactt ctgaacgtcg ccttgaagac cgtctaaggg
     3661 ttttctgatt tggagttatc tgaacctgac tatcctcatt taccatttga tctgctgata
     3721 ttactactgg gggtgtattc tctttggatt ccaaattaat ttctacattt ttctcctcta
     3781 cagtattagt ttccaataat acagcatttt ccattgttgc tgctttaagc tttgtattgt
     3841 catgctccat tgtagattca gaaagatgaa cctgattatc tgacacacat tctgtttgat
     3901 taagcaaacc aggtgggtta ttttctaaga ctacaatgcc ttcaacagat tcctcagtat
     3961 ttttctccgg ctcagatctc attaagggct tagacctttt attccctgtt tgttcagctt
     4021 taccagctct ccggcttgat ctcttagttc cattcactat tttttcagaa tcagattttg
     4081 aaaaagtccc ttctctttgc tttgcttttg gtagaaaatc tgttttaatc atggtttcct
     4141 ggttattttt cactgtaaca gttgatgaaa tattattcaa gggggatgga ctaaaaggtc
     4201 tattttctga accatcaaac ttctccaaag taataaaggt ttgcctccga cttgtagggt
     4261 atgggggagt tccagcaaca gtggtattag aaactgaact actgctgaca acgaaagaat
     4321 tttctgtact agatgcacat tcagttgatg ttttccttga tgaaattaaa gctttttttc
     4381 tttcatcatt attactgttt gacatttctg gactggtttt gtcaagagaa ccacactcat
     4441 tattcgaaag ggaggacttt tcaagatgtt cagccatacc acagtcttcc gtgacatctt
     4501 gaggaatgac aatgtcactg tccatttgct cctccgtaat catcatctta gaatcctcct
     4561 ttggttttct ggttaaagta ggaatttcca taggctcttc ctgactatac tgagtaaata
     4621 aggtatcttg ggaaacatcc agattattat acatggcagg aatatcacac cgctttgttt
     4681 tgagaacttc tttttgatga tcagttaata ttctttcctt tgcatctttt ccttctggag
     4741 gtataaacac aaagtcagta gacttttcct cttccaaaag aatttcaccc tttactttta
     4801 aagacgaaga ttcaagtttc agtttggctg ctggtttcat attttctttt ttattctttg
     4861 tttgtgccaa aaatgaatct ctttttccat ttgattttct ctccatgcca cttatcttca
     4921 catttagttg tgaattttct gttccatcag aatatggtcc actggattcc tccatcattt
     4981 caacagtttc caaaccaggc aacaggagca gaaatttttg tttggcttgt gttagtactg
     5041 gttttaactc ttcaggataa accaacatca tcactttggc aaaagtggca ttccagaact
     5101 gagcactctg ttttcgaatc tgtttattct tgtgcagaaa tattatgcat aatagtgggg
     5161 agagttgttc aagaagttca ctatcataag ttccggtgta gctgaattgc agacaagcaa
     5221 taatttctcc cagtagcttt tctaacttgt tgttcagaca actatatact ttaggaactt
     5281 catcaagctt tgagttttca taaaataatg ccagaggtct tgttaaagtt gcaaatattt
     5341 ttcggatcat agaaggcaaa gaaatatgcc caagtacact ggaaataatg ctggtgattg
     5401 agttgccaat agtgaagagg gtatcagaat gtgcttcctt gaagctcagt gtgtggaaag
     5461 aatagatcac tttcacaata agtttaaata aagaagtcaa tttccctagg ggctcattct
     5521 tctttttgga ccaatctgaa ggtctctgtg gtgatttaac tttgggctga tatttaatat
     5581 tatatggtga gaagtcaatg caatcaacca ttacagtaat aatataaata attctatcca
     5641 cgaacaacaa attagaaaag ccttcatctt ccaaactgga cattatcttg gaagaaagtt
     5701 cctcacagca caagttctct tctgctgttg ccaccaaagc agcacaacga gcaaatgctc
     5761 tatataactc tgaccaagtt ctaagcaaag tcttcatggt ggccactgga aatctctgtt
     5821 ctgaaaaaat gtggtttact ggtaaagtca atgcaccata gatggcacta aaattatgtt
     5881 ctaaggcatc accttgattt acttcattgg tctgattaat caattcagtt aatggggtga
     5941 ctataacact ccacattttc cagagatgct ccttattttc caactgcttt gcattttgat
     6001 taataacatt taaaactgag tcactgaaag ctagtggtga agttggacca gaaagaacac
     6061 agcctacaag tgattccaaa ctgagaaaga acctttcatc tgatacacca cattccaaga
     6121 aattgttgaa aattaattga attaagaaca aagctggagt tccattaaga atatccatat
     6181 tagcaacctg atatgctggt gaacctaata ctttctgagg aagtccttta attgtaattt
     6241 ccatgaggac cagcgttttt gatacaggaa atacttcaga ctttactatg ctttccaaag
     6301 actttaataa gagagtcaaa acttcagaac ctggtttctc ttttttgtta cctgattcag
     6361 taactgactt caccaagcta attagctcct tccagatagc actgacaacc acatcggggg
     6421 catcttttcc aactgcaaca aagctatcat gaacagcagt gataagtgta tttgcatgtt
     6481 tggaaaaaaa ggaagggctg ctgattaacg gatgttccaa tggctctaag ctcagcacaa
     6541 gtttattttg cttagcaaaa ctcaaggctt ctggacccaa caagaaatga agcaacattt
     6601 caagtcccaa aagttgaatg gatgggattg tggccattcc acctaaattc gaactcaggt
     6661 tcattcgggg agttcccggg gctccgtacg gggaggaagc acctttgtgt acaggagtca
     6721 taggatttaa acctggagat gtagctacat gacacgaatt gccctgaggt gaggcattag
     6781 aatcaatgct tattgtactt tgaatcagag gcacacaaac ctgttcaaaa ttagcaggaa
     6841 gatgaggtcc aagtctcatc agtaaatacc accagacttc tagttttgtt aatgctagag
     6901 tttctgttct cacatggatg gaactcaaag gctgcattaa caacttgagt ctttttgcac
     6961 tacatagtat atctggattt aaagcaaaat tatctattaa actcttccaa gcaataaaag
     7021 ctatcttttt aatcatgggt gctccactac gaaatccaag ttcttctagt tgcaagagag
     7081 aattgatgaa actcccactt cgatgcaagg tccttccaag tagtttgaca aacaaaggcc
     7141 ataattttaa cacgtaagtc tcatttttac tcataaatag cttctgaagt tctgagatta
     7201 atttagtagt cataagctgc tccgtaatag atgctatttc ttgctgtttc tgaagcaata
     7261 atggcattcc catctccaga gcagttgctc cccgcaaatg taccttttgt gctgaatgaa
     7321 ccactaaagg tatgaccagt tttgcccacc tcactgcctc ttctcccatt tgaattgggg
     7381 cttgttcaat tagccttacg ataacattta atgcttcaaa atcaacaaca gcagaatgcg
     7441 tctctccttt gttaaacagt atttctaatg aatcaattat actggatacc attttgccaa
     7501 ccacttcaga gggaaatgtc tgcttagata tcacccaaag tgctctagta cgtacatttt
     7561 tgtctgaatt cttaatggta tcattcaatt ttgaaagcaa ttctagagca tttgcctctg
     7621 ataattctga ggtaattttg ggattatata agcaaaaccc cagggcttgt agagcagcac
     7681 tactcagctc cgagttttga ctggaaatgt gagtctttaa aactttgtac agccgaggaa
     7741 gttttttctc aatttctgta attacttctt ttccttcttc tccagtcata cgactggtca
     7801 gagtcaggta agcgtcagtc tgccctccat gggaggcaga agggtcttcc aaagtctcca
     7861 acagcggcgc gagggggctc tgacccctgg ccgtcatggt gaagcctgct tttttgtaca
     7921 aacttgtggg cgcgcccttg tacagctcgt ccatgccgag agtgatcccg gcggcggtca
     7981 cgaactccag caggaccatg tgatcgcgct tctcgttggg gtctttgctc agggcggact
     8041 gggtgctcag gtagtggttg tcgggcagca gcacggggcc gtcgccgatg ggggtgttct
     8101 gctggtagtg gtcggcgagc tgcacgctgc cgtcctcgat gttgtggcgg atcttgaagt
     8161 tcaccttgat gccgttcttc tgcttgtcgg ccatgatata gacgttgtgg ctgttgtagt
     8221 tgtactccag cttgtgcccc aggatgttgc cgtcctcctt gaagtcgatg cccttcagct
     8281 cgatgcggtt caccagggtg tcgccctcga acttcacctc ggcgcgggtc ttgtagttgc
     8341 cgtcgtcctt gaagaagatg gtgcgctcct ggacgtagcc ttcgggcatg gcggacttga
     8401 agaagtcgtg ctgcttcatg tggtcggggt agcggctgaa gcactgcacg ccgtaggtca
     8461 gggtggtcac gagggtgggc cagggcacgg gcagcttgcc ggtggtgcag atgaacttca
     8521 gggtcagctt gccgtaggtg gcatcgccct cgccctcgcc ggacacgctg aacttgtggc
     8581 cgtttacgtc gccgtccagc tcgaccagga tgggcaccac cccggtgaac agctcctcgc
     8641 ccttgctcac catggtacca agcttaagtt taaacgctag agtccggagg ctggatcggt
     8701 cccggtgtct tctatggagg tcaaaacagc gtggatggcg tctccaggcg atctgacggt
     8761 tcactaaacg agctcgtcga cgatctctat cactgatagg gagatctcta tcactgatag
     8821 ggagagctct gcttatatag acctcccacc gtacacgcct accgcccatt tgcgtcaatg
     8881 gggcggagtt gttacgacat tttggaaagt cccgttgatt ttggtgccaa aacaaactcc
     8941 cattgacgtc aatggggtgg agacttggaa atccccgtga gtcaaaccgc tatccacgcc
     9001 cattgatgta ctgccaaaac cgcatcacca tggtaatagc gatgactaat acgtagatgt
     9061 actgccaagt aggaaagtcc cataaggtca tgtactgggc ataatgccag gcgggccatt
     9121 taccgtcatt gacgtcaata gggggcgtac ttggcatatg atacacttga tgtactgcca
     9181 agtgggcagt ttaccgtaaa tactccaccc attgacgtca atggaaagtc cctattggcg
     9241 ttactatggg aacatacgtc attattgacg tcaatgggcg ggggtcgttg ggcggtcagc
     9301 caggcgggcc atttaccgta agttatgtaa cgcggaactc catatatggg ctatgaacta
     9361 atgaccccgt aattgattac tattaataac tagtcaataa tcaatgtcaa cgcgtatatc
     9421 tggcccgtac atcgcgaagc agcgcaaaac gcctaaccct aagcagattc ttcatgcaat
     9481 tgtcggtcaa gccttgcctt gttgtagctt aaattttgct cgcgcactac tcagcgacct
     9541 ccaacacaca agcagggagc agatactggc ttaactatgc ggcatcagag cagattgtac
     9601 tgagagtgca ccatagggga tcgggagatc tcccgatccg tcgacgtcag gtggcacttt
     9661 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     9721 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     9781 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     9841 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     9901 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     9961 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
    10021 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
    10081 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
    10141 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
    10201 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
    10261 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
    10321 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
    10381 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
    10441 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
    10501 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
    10561 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
    10621 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
    10681 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
    10741 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
    10801 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
    10861 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
    10921 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
    10981 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
    11041 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
    11101 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
    11161 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
    11221 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
    11281 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
    11341 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
    11401 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
    11461 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
    11521 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
    11581 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
    11641 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
    11701 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
    11761 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagctcta
    11821 gctagaggtc gacggtatac agacatgata agatacattg atgagtttgg acaaaccaca
    11881 actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt
    11941 gtaaccatta taagctgcaa taaacaagtt ggggtgggcg aagaactcca gcatgagatc
    12001 cccgcgctgg aggatcatcc agccggcgtc ccggaaaacg attccgaagc ccaacctttc
    12061 atagaaggcg gcggtggaat cgaaatctcg tagtacgtgc tattcctttg ccctcggacg
    12121 agtgctgggg cgtcggtttc cactatcggc gagtacttct acacagccat cggtccagac
    12181 ggccgcgctt ctgcgggcga tttgtgtacg cccgacagtc ccggctccgg atcggacgat
    12241 tgcgtcgcat cgaccctgcg cccaagctgc atcatcgaaa ttgccgtcaa ccaagctctg
    12301 atagagttgg tcaagaccaa tgcggagcat atacgcccgg agccgcggcg atcctgcaag
    12361 ctccggatgc ctccgctcga agtagcgcgt ctgctgctcc atacaagcca accacggcct
    12421 ccagaagaag atgttggcga cctcgtattg ggaatccccg aacatcgcct cgctccagtc
    12481 aatgaccgct gttatgcggc cattgtccgt caggacattg ttggagccga aatccgcgtg
    12541 cacgaggtgc cggacttcgg ggcagtcctc ggcccaaagc atcagctcat cgagagcctg
    12601 cgcgacggac gcactgacgg tgtcgtccat cacagtttgc cagtgataca catggggatc
    12661 agcaatcgcg catatgaaat cacgccatgt agtgtattga ccgattcctt gcggtccgaa
    12721 tgggccgaac ccgctcgtct ggctaagatc ggccgcagcg atcgcatcca tggcctccgc
    12781 gaccggctgc agaacagcgg gcagttcggt ttcaggcagg tcttgcaacg tgacaccctg
    12841 tgcacggcgg gagatgcaat aggtcaggct ctcgctgaac tccccaatgt caagcacttc
    12901 cggaatcggg agcgcggccg atgcaaagtg ccgataaaca taacgatctt tgtagaaacc
    12961 atcggcgcag ctatttaccc gcaggacata tccacgccct cctacatcga agctgaaagc
    13021 acgagattct tcgccctccg agagctgcat caggtcggag acgctgtcga acttttcgat
    13081 cagaaacttc tcgacagacg tcgcggtgag ttcaggcttt ttggccaagg aagttcctat
    13141 actttctaga gaataggaac ttcggaatag gaacttctag gtacgtgaac catcacccta
    13201 at
//
