LOCUS       sequence-372906-        9979 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for surface expressed EGFP (SFFV promoter).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9979)
  AUTHORS   Morsut L, Roybal KT, Xiong X, Gordley RM, Coyle SM, Thomson M, Lim 
            WA
  TITLE     Engineering Customized Cell Sensing and Response Behaviors Using 
            Synthetic Notch Receptors.
  JOURNAL   Cell. 2016 Feb 11;164(4):780-91. doi: 10.1016/j.cell.2016.01.012. 
            Epub 2016 Jan 28.
  PUBMED    26830878
REFERENCE   2  (bases 1 to 9979)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.01.012"; journalName: "Cell"; date: 
            "2016-02-11- 11"; volume: "164"; issue: "4"; pages: "780-91"
COMMENT     Sequence Label: pHR_EGFPligand
FEATURES             Location/Qualifiers
     source          1..9979
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     141..161
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     primer_bind     complement(231..248)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(402..990)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(482..501)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1161..1952,1953..2021))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1784..1803
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2022..2126)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2194..2212
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2250..2272)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2372..2391
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2605..2624
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        2914..2932
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2914..2931
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     LTR             2957..3190
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     3280..3296
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     3281..3297
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    3300..3888
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             3406..3417
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     3815..3835
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(join(4151..4861,4862..4864,4865..4867))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     regulatory      4927..4936
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(5004..5411)
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     misc_feature    5564..5681
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(5966..6010)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6195..6428
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    6925..7050
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             7097..7730
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     polyA_signal    7898..8032
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7922..7941)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7976..7995
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(8457..8477)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          8607..8672
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     promoter        complement(join(9806..9979,1..156))
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9820..9955
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 ggttgctgac taattgagat gcatgctttg catacttctg cctgctgggg agcctgggga
       61 ctttccacac ctggttgctg actaattgag atgcatgctt tgcatacttc tgcctgctgg
      121 ggagcctggg gactttccac accctaactg acacacattc cacagctgca ttaatgaatc
      181 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
      241 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
      301 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
      361 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
      421 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
      481 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
      541 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
      601 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
      661 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
      721 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
      781 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
      841 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
      901 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
      961 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     1021 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     1081 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     1141 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     1201 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     1261 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     1321 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     1381 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     1441 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     1501 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     1561 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     1621 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     1681 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     1741 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     1801 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     1861 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     1921 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     1981 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     2041 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     2101 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa
     2161 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt
     2221 ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc
     2281 acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt
     2341 gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg
     2401 caccattcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt
     2461 gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag
     2521 tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa
     2581 gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc
     2641 tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatctggc tagcgatgac
     2701 cctgctgatt ggttcgctga ccatttccgg gtgcgggacg gcgttaccag aaactcagaa
     2761 ggttcgtcca accaaaccga ctctgacggc agtttacgag agagatgata gggtctgctt
     2821 cagtaagcca gatgctacac aattaggctt gtacatattg tcgttagaac gcggctacaa
     2881 ttaatacata accttatgta tcatacacat acgatttagg tgacactata gaatacacgg
     2941 aattaattct agatgctgct agagattttc cacactgact aaaagggtct gagggatctc
     3001 tagttaccag agtcacacaa cagacgggca cacactactt gaagcactca aggcaagctt
     3061 tattgaggct taagcagtgg gttccctagt tagccagaga gctcccaggc tcagatctgg
     3121 tctaaccaga gagacccagt acaagcaaaa agcagatctt gtcttcgttg ggagtgaatt
     3181 agcccttcca gtcccccctt ttcttttaaa aagtggctaa gatctacagc tgccttgtaa
     3241 gtcattggtc ttaaaggtac cgagctcgaa ttaattccct cgaggtcgac ggtatcgatg
     3301 cggggaggcg gcccaaaggg agatccgact cgtctgaggg cgaaggcgaa gacgcggaag
     3361 aggccgcaga gccggcagca ggccgcggga aggaaggtcc gctggattga gggccgaagg
     3421 gacgtagcag aaggacgtcc cgcgcagaat ccaggtggca acacaggcga gcagccaagg
     3481 aaaggacgat gatttccccg acaacaccac ggaattgtca gtgcccaaca gccgagcccc
     3541 tgtccagcag cgggcaaggc aggcggcgat gagttccgcc gtggcaatag ggagggggaa
     3601 agcgaaagtc ccggaaagga gctgacaggt ggtggcaatg ccccaaccag tgggggttgc
     3661 gtcagcaaac acagtgcaca ccacgccacg ttgcctgaca acgggccaca actcctcata
     3721 aagagacagc aaccaggatt tatacaagga ggagaaaatg aaagccatac gggaagcaat
     3781 agcatgatac aaaggcatta aagcagcgta tccacatagc gtaaaaggag caacatagtt
     3841 aagaatacca gtcaatcttt cacaaatttt gtaatccaga ggttgattat cgataagctt
     3901 gatatcaagc ttgcatgcct gcaggtcgac tctagagtcg cggcctcagt cagtcacgca
     3961 tgttgcaggt gggagttgcg gccgcaagtc aaggatctta tcggggtttt ttttgccaca
     4021 gcatgataag tatgataagg gagataatag tcagcaccac caacgccagg atagcactaa
     4081 taaccacgac cttgaagggc aagctgtggg gcacgacgat cacctcctgc gtatcctggc
     4141 ccacggcgtt cttatacagc tcatccattc ccagggtgat cccagcagcc gtcacgaatt
     4201 caagcagaac catatggtct cttttttcgt taggatcctt ggagagggct gattgcgtgg
     4261 agaggtagtg gttatctggc agcaaaaccg gaccgtcgcc gataggcgtg ttttgctggt
     4321 aatggtcggc cagttgcaca gagccatcct cgatattgtg tcggattttg aagttcacct
     4381 tgatgccgtt cttctgctta tcagccataa tatacacgtt gtggctgttg tagttgtact
     4441 ccagcttgtg gcccaaaata ttgccgtctt ctttgaaatc gattcctttc aactcaattc
     4501 tatttaccaa cgtgtccccc tcgaacttaa cttcagccct ggttttatag ttaccgtcgt
     4561 ccttgaagaa tatggtgcgt tcctgaacat agccctcagg catcgcggac ttgaagaaat
     4621 cgtgctgttt catgtggtcg gggtaccggg agaagcactg gactccgtag gtgagggtgg
     4681 tcacgagtgt aggccagggc actggcagct ttcctgttgt acagatgaac ttcaacgtca
     4741 gtttaccgta cgtagcgtcg ccctctcctt cgccgctcac tgagaacttg tgtccgttaa
     4801 cgtctccgtc cagctcaacc aggatgggca caactccggt gaacaattct tcacccttag
     4861 aaaccatgtc tccggtacta cccggaaccc acagcagcag gacccagagc aagagggtgt
     4921 cggtctccat ggtggcggcg atccactgtc cacctgcctt gctccacttg acgcgtgaga
     4981 attctcgaga gctccagatc ccccccgggc gactcagtct gtcggaggac tggcgcgccg
     5041 agtgaggggt tgtgagctct tttatagagc tcgggaagca gaagcgcgcg aacagaagcg
     5101 agaagcaggc tgattggtta attcaaataa ggcgcagggt catttcaggt ccttggggga
     5161 gcctggaaac atctgatggg tcttaagaaa ctgctgaggg ttgggccata tctggggacc
     5221 atctgttctt ggcctcgggc cggggccgaa actgcggtga ccatctgttc ttggccccgg
     5281 gccggggccg aaactgctca ccgcagatat cctgtttggc ccaacgttag ctattttcat
     5341 gtacccgccc ttgatctgaa cttctctatt cttggtttgg tatttttcca tgccttgcaa
     5401 aatggcgtta ctgcagctag cttgccaaac ctacaggtgg ggtctttcat tccccccttt
     5461 ttctggagac taaataaaat cttttatttt atcggggctg caggaattcg atatcaagct
     5521 tatcgatcca aactggatct ctgctgtccc tgtaataaac ccgaaaattt tgaatttttg
     5581 taatttgttt ttgtaattct ttagtttgta tgtctgttgc tattatgtct actattcttt
     5641 cccctgcact gtacccccca atcccccctt ttcttttaaa attgtggatg aatactgcca
     5701 tttggcgata ccgtcgagat ccgttcacta atcgaatgga tctgtctctg tctctctctc
     5761 caccttcttc ttctattcct tcgggcctgt cgggtcccct cggggttggg aggtgggtct
     5821 gaaacgataa tggtgaatat ccctgcctaa ctctattcac tatagaaagt acagcaaaaa
     5881 ctattcttaa acctaccaag cctcctacta tcattatgaa taattttata taccacagcc
     5941 aatttgttat gttaaaccaa ttccacaaac ttgcccattt atctaattcc aataattctt
     6001 gttcattctt ttcttgctgg ttttgcgatt cttcaattaa ggagtgtatt aagcttgtgt
     6061 aattgttaat ttctctgtcc cactccatcc aggtcgtgtg attccaaatc tgttccagag
     6121 atttattact ccaactagca ttccaaggca cagcagtggt gcaaatgagt tttccagagc
     6181 aaccccaaat ccccaggagc tgttgatcct ttaggtatct ttccacagcc aggattcttg
     6241 cctggagctg cttgatgccc cagactgtga gttgcaacag atgctgttgc gcctcaatag
     6301 ccctcagcaa attgttctgc tgctgcacta taccagacaa taattgtctg gcctgtaccg
     6361 tcagcgtcat tgacgctgcg cccatagtgc ttcctgctgc tcccaagaac ccaaggaaca
     6421 aagctcctat tcccactgct cttttttctc tctgcaccac tcttctcttt gccttggtgg
     6481 gtgctactcc taatggttca atttttacta ctttatattt atataattca cttctccaat
     6541 tgtccctcat atctcctcct ccaggtctga agatcagcgg ccggccgctt gctgtgcggt
     6601 ggtcttactt ttgttttgct cttcctctat cttgtctaaa gcttccttgg tgtcttttat
     6661 ctctatcctt tgatgcacac aatagagggt tgctactgta ttatataatg atctaagttc
     6721 ttctgatcct gtctgaaggg atggttgtag ctgtcccagt atttgtctac agccttctga
     6781 tgtttctaac aggccaggat taactgcgaa tcgttctagc tccctgcttg cccatactat
     6841 atgttttaat ttatattttt tctttccccc tggccttaac cgaatttttt cccatcgcga
     6901 tctaattctc ccccgcttaa tactgacgct ctcgcaccca tctctctcct tctagcctcc
     6961 gctagtcaaa atttttggcg tactcaccag tcgccgcccc tcgcctcttg ccgtgcgcgc
     7021 ttcagcaagc cgagtcctgc gtcgagagag ctcctctggt ttccctttcg ctttcaagtc
     7081 cctgttcggg cgccactgct agagattttc cacactgact aaaagggtct gagggatctc
     7141 tagttaccag agtcacacaa cagacgggca cacactactt gaagcactca aggcaagctt
     7201 tattgaggct taagcagtgg gttccctagt tagccagaga gctcccaggc tcagatctgg
     7261 tctaaccaga gagacccagt acaggcaaaa agcagctgct tatatgcagg atctgagggc
     7321 tcgccactcc ccagtcccgc ccaggccacg cctccctgga aagtccccag cggaaagtcc
     7381 cttgtagcaa gctcgatatc agcagttctt gaagtactcc ggatgcagct ctcgggccac
     7441 gtgatgaaat gctaggcggc tgtcaaacct ccactctaac acttctctct ccgggtcatc
     7501 catcccatgc aggctcacag ggtgtaacaa gctggtgttc tctcctttat tggcctcttc
     7561 taccttatct ggctcaactg gtactagctt gtagcaccat ccaaaggtca gtggatatct
     7621 gacccctggc cctggtgtgt agttctgcta atcagggaag tagccttgtg tgtggtagat
     7681 ccacagatca aggatatctt gtcttctttg ggagtgaatt agcccttcca actactaagt
     7741 ttgtagtaca tatttaacaa atacaatttc tttaaaatga aaataattca gaggaatcac
     7801 aggtttagag taaatgaaac cacaggtaat tggcagtggt aatagggtat ggggtgggaa
     7861 gtttgggatg attttggtta gcttgagtta tccagttgat ccagacatga taagatacat
     7921 tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat
     7981 ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa
     8041 caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaaagcaa
     8101 gtaaaacctc tacaaatgtg gtatggctga ttatgatctc tagtcaaggc actatacatc
     8161 aaatattcct tattaacccc tttacaaatt aaaaagctaa aggtacacaa tttttgagca
     8221 tagttattaa tagcagacac tctatgcctg tgtggagtaa gaaaaaacag tatgttatga
     8281 ttataactgt tatgcctact tataaaggtt acagaatatt tttccataat tttcttgtat
     8341 agcagtgcag ctttttcctt tgtggtgtaa atagcaaagc aagcaagagt tctattacta
     8401 aacacagcat gactcaaaaa acttagcaat tctgaaggaa agtccttggg gtcttctacc
     8461 tttctcttct tttttggagg agtagaatgt tgagagtcag cagtagcctc atcatcacta
     8521 gatggcattt cttctgagca aaacaggttt tcctcattaa aggcattcca ccactgctcc
     8581 cattcatcag ttccataggt tggaatctaa aatacacaaa caattagaat cagtagttta
     8641 acacattata cacttaaaaa ttttatattt accttagagc tttaaatctc tgtaggtagt
     8701 ttgtccaatt atgtcacacc acagaagtaa ggttccttca caaagatccg gggcccactc
     8761 ataaatccag ttgccgccac ggtagccaat caccgtatcg tataaatcat cgtcggtacg
     8821 ttcggcatcg ctcatcacaa tacgtgcctg gacgtcgagg atttcgcgtg ggtcaatgcc
     8881 gcgccagatc cacatcagac ggttaatcat gcgataccag tgagggatgg ttttaccatc
     8941 aagggccgac tgcacaggcg gttgtgcgcc gtgattaaag cggcggacta gcgtcgaggt
     9001 ttcaggatgt ttaaagcggg gtttgaacag ggtttcgctc aggtttgcct gtgtcatgga
     9061 tgcagcctcc agaatactta ctggaaacta ttgtaacccg cctgaagtta aaaagaacaa
     9121 cgcccggcag tgccaggcgt tgaaaagatt agcgaccgga gattggcggg acgaatacga
     9181 cgcccatatc ccacggctgt tcaatccagg tatcttgcgg gatatcaaca acatagtcat
     9241 caaccagcgg acgaccagcc ggttttgcga agatggtgac aaagtgcgct tttggataca
     9301 tttcacgaat cgcaaccgca gtaccaccgg tatccaccag gtcatcaata acgatgaagc
     9361 cttcgccatc gccttctgcg cgtttcagca ctttaagctc gcgctggttg tcgtgatcgt
     9421 agctggaaat acaaacggta tcgacatgac gaatacccag ttcacgcgcc agtaacgcac
     9481 ccggtacaga ccgccacggc ttacggcaat aatgcctttc cattgttcag aaggcatcag
     9541 tcggcttgcg agtttacgtg catggatctg caacatgtcc caggtgacga tgtatttttc
     9601 gctcatgtga agtgtcccag cctgtttatc tacggcttaa aaagtgttcg aggggaaaat
     9661 aggttgcgcg agattataga gatctggcgc actaaaaacc agtatttcac atgagtccgc
     9721 gtctttttac gcactgcctc tccctgacgc gggataaagt ggtattctca aacatatctc
     9781 gcaagcctgt cttgtgtcca agctttttgc aaaagcctag gcctccaaaa aagcctcctc
     9841 actacttctg gaatagctca gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa
     9901 ttagtcagcc atggggcgga gaatgggcgg aactgggcgg agttaggggc gggatgggcg
     9961 gagttagggg cgggactat
//
