LOCUS       sequence-372912-        8174 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lentiviral expression of Bcl-XL.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8174)
  AUTHORS   Cheng Z, Gong Y, Ma Y, Lu K, Lu X, Pierce LA, Thompson RC, Muller S,
            Knapp S, Wang J
  TITLE     Inhibition of BET bromodomain targets genetically diverse 
            glioblastoma.
  JOURNAL   Clin Cancer Res. 2013 Apr 1;19(7):1748-59. doi: 
            10.1158/1078-0432.CCR-12-3066. Epub 2013 Feb 12.
  PUBMED    23403638
REFERENCE   2  (bases 1 to 8174)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/1078-0432.CCR-12-3066"; journalName: "Clin Cancer Res"; 
            date: "2013-04-1- 1"; volume: "19"; issue: "7"; pages: "1748-59"
COMMENT     Sequence Label: pCDH-puro-Bcl-XL
FEATURES             Location/Qualifiers
     source          1..8174
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     385..402
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    519..540
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(555..560,561..578,579..585)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    593..609
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     598..620
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     617..633
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     617..633
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      666..801
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    841..962
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(852..871)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     906..925
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             1034..1267
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    1341..1929
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             1447..1458
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     1856..1876
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1936..2535)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2019..2039)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     2516..2535
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     LTR             2562..2830
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     promoter        complement(2843..3054)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     primer_bind     complement(3945..3969)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(3966..4169)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(3995..4015)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    4259..4375
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(4642..4686)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4871..5104
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    5597..5722
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             5769..5949
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(5950..6176)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(6191..6208)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6191..6207)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6200..6222)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(6416..6435)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6535..6557
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6595..6613)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6681..6785
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6786..6854,6855..7646)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7004..7023)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(7817..8174,1..231)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
       61 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
      121 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
      181 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
      241 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
      301 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
      361 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
      421 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
      481 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat
      541 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
      601 ggataacaat ttcacacagg aaacagctat gacatgatta cgaatttggt ctctgcaaaa
      661 gtctaggcct ccaaaaaagc ctcctcacta cttctggaat agctcagagg ccgaggcggc
      721 ctcggcctct gcataaataa aaaaaattag tcagccatgg ggcggagaat gggcggaact
      781 gggcggagtt aggggcggga tgggcggagt taggggcggg atagctagag ccagacatga
      841 taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta
      901 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag
      961 ttcctctcac tctctgatat tcatttcttt gcaagttata aatactgaat aataagatga
     1021 catgaactac tactgctaga gattttccac actgactaaa agggtctgag ggatctctag
     1081 ttaccagagt cacacaacag acgggcacac actacttgaa gcactcaagg caagctttat
     1141 tgaggcttaa gcagtgggtt ccctagttag ccagagagct cccaggctca gatctggtct
     1201 aaccagagag acccagtaca agcaaaaagc agatcttatc ttcgttggga gtgaattagc
     1261 ccttccagtc cccccttttc ttttaaaaag tggctaagat ctacagctgc cttgtaagtc
     1321 attggtctta aaggtaccag gcggggaggc ggcccaaagg gagatccgac tcgtctgagg
     1381 gcgaaggcga agacgcggaa gaggccgcag agccggcagc aggccgcggg aaggaaggtc
     1441 cgctggattg agggccgaag ggacgtagca gaaggacgtc ccgcgcagaa tccaggtggc
     1501 aacacaggcg agcagccaag gaaaggacga tgatttcccc gacaacacca cggaattgtc
     1561 agtgcccaac agccgagccc ctgtccagca gcgggcaagg caggcggcga tgagttccgc
     1621 cgtggcaata gggaggggga aagcgaaagt cccggaaagg agctgacagg tggtggcaat
     1681 gccccaacca gtgggggttg cgtcagcaaa cacagtgcac accacgccac gttgcctgac
     1741 aacgggccac aactcctcat aaagagacag caaccaggat ttatacaagg aggagaaaat
     1801 gaaagccata cgggaagcaa tagcatgata caaaggcatt aaagcagcgt atccacatag
     1861 cgtaaaagga gcaacatagt taagaatacc agtcaatctt tcacaaattt tgtaatccag
     1921 aggttgattg tcgactcagg caccgggctt gcgggtcatg caccaggtgc gcggtccttc
     1981 gggcacctcg acgtcggcgg tgacggtgaa gccgagccgc tcgtagaagg ggaggttgcg
     2041 gggcgcggag gtctccagga aggcgggcac cccggcgcgc tcggccgcct ccactccggg
     2101 gagcacgacg gcgctgccca gacccttgcc ctggtggtcg ggcgagacgc cgacggtggc
     2161 caggaaccac gcgggctcct tgggccggtg cggcgccagg aggccttcca tctgttgctg
     2221 cgcggccagc cgggaaccgc tcaactcggc catgcgcggg ccgatctcgg cgaacaccgc
     2281 ccccgcttcg acgctctccg gcgtggtcca gaccgccacc gcggcgccgt cgtccgcgac
     2341 ccacaccttg ccgatgtcga gcccgacgcg cgtgaggaag agttcttgca gctcggtgac
     2401 ccgctcgatg tggcggtccg gatcgacggt gtggcgcgtg gcggggtagt cggcgaacgc
     2461 ggcggcgagg gtgcgtacgg ccctggggac gtcgtcgcgg gtggcgaggc gcaccgtggg
     2521 cttgtactcg gtcatctagc gtaggcgccg gtcacagctt ggatctgtaa cggcgcagaa
     2581 cagaaaacga aacaaagacg tagagttgag caagcagggt caggcaaagc gtggagagcc
     2641 ggctgagtct aggtaggctc caagggagcg ccggacaaag gcccggtctc gacctgagct
     2701 ttaaacttac ctagacggcg gacgcagttc aggaggcacc acaggcggga ggcggcagaa
     2761 cgcgactcaa ccggcgtgga tggcggcctc aggtagggcg gcgggcgcgt gaaggagaga
     2821 tgcgagcccc tcgaagcttc agctgtgttc tggcggcaaa cccgttgcga aaaagaacgt
     2881 tcacggcgac tactgcactt atatacggtt ctcccccacc ctcgggaaaa aggcggagcc
     2941 agtacacgac atcactttcc cagtttaccc cgcgccacct tctctaggca cccgttcaat
     3001 tgccgacccc tccccccaac ttctcgggga ctgtgggcga tgtgcgctct gcccactgac
     3061 gggcaccgga gcgatcgcag atccttcgcg gccgcggatc cgatttaaat tcgaattcgt
     3121 agagtggatg gtcagtgtct ggtcatttcc gactgaagag tgagcccagc agaaccacgc
     3181 cggccacagt catgcccgtc aggaaccagc ggttgaagcg ttcctggccc tttcggctct
     3241 cggctgctgc attgttccca tagagttcca caaaagtatc ccagccgccg ttctcctgga
     3301 tccaaggctc taggtggtca ttcaggtaag tggccatcca agctgcgatc cgactcacca
     3361 atacctgcat ctccttgtct acgctttcca cgcacagtgc cccgccgaag gagaaaaagg
     3421 ccacaatgcg accccagttt accccatccc ggaagagttc attcactacc tgttcaaagc
     3481 tctgatatgc tgtccctggg gtgatgtgga gctgggatgt caggtcactg aatgcccgcc
     3541 ggtaccgcag ttcaaactcg tcgcctgcct ccctcagcgc ttgctttact gctgccatgg
     3601 ggatcacctc ccgggcatcc aaactgctgc tgtggccagt ggctccattc accgcggggc
     3661 tgtctgccag gtgccaggat gggttgccat tgatggcact gggggtctcc atctccgatt
     3721 cagtcccttc tggggcctca gtcctgttct cttccacatc actaaactga ctccagctgt
     3781 atcctttctg ggaaagcttg taggagagaa agtcaaccac cagctcccgg ttgctctgag
     3841 acatttttat aatagggatg ggctcaacca gtccattgtc caagaattcg ctagctctag
     3901 aatcttctat ggaggtcaaa acagcgtgga tggcgtctcc aggcgatctg acggttcact
     3961 aaacgagctc tgcttatata aacctcccac cgtacacgcc taccgcccat ttgcgtcaat
     4021 ggggcggagt tgttacgaca ttttggaaag tcccgttgat tttggtgcca aaacaaactc
     4081 ccattgacgt caatggggtg gagacttgga aatccccgtg agtcaaaccg ctatccacgc
     4141 ccattgatgt actgccaaaa ccgcatcacc atggtaatag cgatgactaa tacgtagatg
     4201 tactgccaag taggaaagtc ccataaggtc atgtactggg cataatacta gtatcgataa
     4261 aattttgaat tttgtaattt gtttttgtaa ttctttagtt tgtatgtctg ttgctattat
     4321 gtctactatt ctttcccctg cactgtaccc cccaatcccc ccttttcttt taaaagttaa
     4381 ccgataccgt cgagatccgt tcactaatcg aatggatctg tctctgtctc tctctccacc
     4441 ttcttcttct attccttcgg gcctgtcggg tcccctcggg gttgggaggt gggtctgaaa
     4501 cgataatggt gaatatccct gcctaactct attcactata gaaagtacag caaaaactat
     4561 tcttaaacct accaagcctc ctactatcat tatgaataat tttatatacc acagccaatt
     4621 tgttatgtta aaccaattcc acaaacttgc ccatttatct aattccaata attcttgttc
     4681 attcttttct tgctggtttt gcgattcttc aattaaggag tgtattaagc ttgtgtaatt
     4741 gttaatttct ctgtcccact ccatccaggt cgtgtgattc caaatctgtt ccagagattt
     4801 attactccaa ctagcattcc aaggcacagc agtggtgcaa atgagttttc cagagcaacc
     4861 ccaaatcccc aggagctgtt gatcctttag gtatctttcc acagccagga ttcttgcctg
     4921 gagctgcttg atgccccaga ctgtgagttg caacagatgc tgttgcgcct caatagccct
     4981 cagcaaattg ttctgctgct gcactatacc agacaataat tgtctggcct gtaccgtcag
     5041 cgtcattgac gctgcgccca tagtgcttcc tgctgctccc aagaacccaa ggaacaaagc
     5101 tcctattccc actgctcttt tttctctctg caccactctt ctctttgcct tggtgggtgc
     5161 tactcctaat ggttcaattt ttactacttt atatttatat aattcacttc tccaattgtc
     5221 cctcatatct cctcctccag gtctgaagat cagtggccgc ttgctgtgcg gtggtcttac
     5281 ttttgttttg ctcttcctct atcttgtcta aagcttcctt ggtgtctttt atctctatcc
     5341 tttgatgcac acaatagagg gttgctactg tattatataa tgatctaagt tcttctgatc
     5401 ctgtctgaag ggatggttgt agctgtccca gtatttgtct acagccttct gatgtttcta
     5461 acaggccagg attaactgcg aatcgttcta gctccctgct tgcccatact atatgtttta
     5521 atttatattt tttctttccc cctggcctta accgaatttt ttcccatcgc gatctaattc
     5581 tcccccgctt aatactgacg ctctcgcacc catctctctc cttctagcct ccgctagtca
     5641 aaatttttgg cgtactcacc agtcgccgcc cctcgcctct tgccgtgcgc gcttcagcaa
     5701 gccgagtcct gcgtcgagag agctcctctg gtttcccttt cgctttcaag tccctgttcg
     5761 ggcgccactg ctagagattt tccacactga ctaaaagggt ctgagggatc tctagttacc
     5821 agagtcacac aacagacggg cacacactac ttgaagcact caaggcaagc tttattgagg
     5881 cttaagcagt gggttcccta gttagccaga gagctcccag gctcagatct ggtctaacca
     5941 gagagacccg tttatgtatc gagctaggca cttaaataca atatctctgc aatgcggcaa
     6001 ttcagtggtt cgtccaatcc atgtcagacc cgtctgttgc cttcctaata aggcacgatc
     6061 gtaccacctt acttccacca atcggcatgc acggtgcttt ttctctcctt gtaaggcatg
     6121 ttgctaactc atcgttacca tgttgcaaga ctacaagagt attgcataag actacacgcg
     6181 tcagcttggc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
     6241 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
     6301 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc ctgatgcggt
     6361 attttctcct tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtacaa
     6421 tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc
     6481 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
     6541 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg
     6601 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
     6661 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     6721 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     6781 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     6841 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     6901 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     6961 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     7021 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     7081 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     7141 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     7201 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     7261 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     7321 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     7381 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     7441 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     7501 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     7561 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     7621 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     7681 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     7741 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     7801 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     7861 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     7921 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     7981 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     8041 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     8101 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     8161 gcttggagcg aacg
//
