LOCUS       sequence-37292-e         755 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 755)
  AUTHORS   Luker KE, Smith MC, Luker GD, Gammon ST, Piwnica-Worms H, 
            Piwnica-Worms D
  TITLE     Kinetics of regulated protein-protein interactions revealed with 
            firefly luciferase complementation imaging in cells and living 
            animals.
  JOURNAL   Proc Natl Acad Sci U S A. 2004 Aug 17. 101(33):12288-93.
  PUBMED    15284440
REFERENCE   2  (bases 1 to 755)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2004 Aug 17. 101(33):12288-93."
COMMENT     Sequence Label: FRB-NLuc
FEATURES             Location/Qualifiers
     source          1..755
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     17..36
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        17..35
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             119..388
                     /codon_start=1
                     /product="FKBP-rapamycin binding domain of human FRAP"
                     /label=FRB
                     /translation="EMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTLKETS
                     FNQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRRISKQ"
     primer_bind     complement(497..515)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 actggcttat cgaaattaat acgactcact atagggagac ccaagctggc tagttaagct
       61 tggtaccgag ctcggatcca ctagtccagt gtggtggaat tgcccttaca ccaccatgga
      121 gatgtggcat gaaggcctgg aagaggcatc tcgtttgtac tttggggaaa ggaacgtgaa
      181 aggcatgttt gaggtgctgg agcccttgca tgctatgatg gaacggggcc cccagactct
      241 gaaggaaaca tcctttaatc aggcctatgg tcgagattta atggaggccc aagagtggtg
      301 caggaagtac atgaaatcag ggaatgtcaa ggacctcacc caagcctggg acctctatta
      361 tcatgtgttc cgacgaatct caaagcagca gatctcgtac gcgtcccggg gcggtggctc
      421 atctggcgga ggtgaagacg ccaaaaacat aaagaaaggc ccggcgccat tctatccgct
      481 ggaagatgga accgctggag agcaactgca taaggctatg aagagatacg ccctggttcc
      541 tggaacaatt gcttttacag atgcacatat cgaggtggac atcacttacg ctgagtactt
      601 cgaaatgtcc gttcggttgg cagaagctat gaaacgatat gggctgaata caaatcacag
      661 aatcgtcgta tgcagtgaaa actctcttca attctttatg ccggtgttgg gcgcgttatt
      721 tatcggagtt gcagttgcgc ccgcgaacga cattt
//
