LOCUS       sequence-372922-        8479 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression plasmid of human IRF8, neomycin selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8479)
  AUTHORS   Cao Z, Budinich KA, Huang H, Ren D, Lu B, Zhang Z, Chen Q, Zhou Y, 
            Huang YH, Alikarami F, Kingsley MC, Lenard AK, Wakabayashi A, 
            Khandros E, Bailis W, Qi J, Carroll MP, Blobel GA, Faryabi RB, Bernt
            KM, Berger SL, Shi J
  TITLE     ZMYND8-regulated IRF8 transcription axis is an acute myeloid 
            leukemia dependency.
  JOURNAL   Mol Cell. 2021 Sep 2;81(17):3604-3622.e10. doi: 
            10.1016/j.molcel.2021.07.018. Epub 2021 Aug 5.
  PUBMED    34358447
REFERENCE   2  (bases 1 to 8479)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2021.07.018"; journalName: "Mol Cell"; date: 
            "2021-09-2- 2"; volume: "81"; issue: "17"; pages: "3604-3622.e10"
COMMENT     Sequence Label: IRF8-2A-neo
FEATURES             Location/Qualifiers
     source          1..8479
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1723..1840
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1909..2120
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      2140..2149
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3469..3525
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3520..4320
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="GPMIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQG
                     RPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEE
                     HQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQD
                     IALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3580..3599)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4190..4209
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    4336..4924
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4389..4409)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4807..4818)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4996..5229
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5307..5428
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5344..5363)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5398..5417
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5468..5603
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5623..5642)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5624..5642)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5652..5669)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5652..5668)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5661..5683)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5810..6265
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5897..5916)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6107..6128
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6291..6395
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6396..6464,6465..7256)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6614..6633)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7427..8015
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7916..7935
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8169..8186
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8303..8324
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8339..8344,8345..8362,8363..8369)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8377..8393
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8382..8404
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8401..8417
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8401..8417
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8436..8456
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8438..8456
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccagaccac aattttaaaa gaaaaggggg
     1741 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     1801 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     1861 cagagatcca ctttggcgcc ggctcgagtg gctccggtgc ccgtcagtgg gcagagcgca
     1921 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga
     1981 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg
     2041 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg
     2101 ggtttgccgc cagaacacag gtgtcgtgac gcgggatccg ccaccatggc aggtgtcccg
     2161 gagtccctga atctgatgtg tgaccggaat ggtggtcggc ggcttcgaca gtggctgatc
     2221 gagcagattg acagtagcat gtatccagga ctgatttggg agaatgagga gaagagcatg
     2281 ttccggatcc cttggaaaca cgctggcaag caagattata atcaggaagt ggatgcctcc
     2341 atttttaagg cctgggcagt ttttaaaggg aagtttaaag aaggggacaa agctgaacca
     2401 gccacttgga agacgaggtt acgctgtgct ttgaataaga gcccagattt tgaggaagtg
     2461 acggaccggt cccaactgga catttccgag ccatacaaag tttaccgaat tgttcctgag
     2521 gaagagcaaa aatgcaaact aggcgtggca actgctggct gcgtgaatga agttacagag
     2581 atggagtgcg gtcgctctga aatcgacgag ctgatcaagg agccttctgt ggacgattac
     2641 atggggatga tcaaaaggag cccttccccg ccggaggcct gtcggagtca gctccttcca
     2701 gactggtggg cgcagcagcc cagcacaggc gtgccgctgg tgacggggta caccacctac
     2761 gacgcgcacc attcagcatt ctcccagatg gtgatcagct tctactatgg gggcaagctg
     2821 gtgggccagg ccaccaccac ctgccccgag ggctgccgcc tgtccctgag ccagcctggg
     2881 ctgcccggca ccaagctgta tgggcccgag ggcctggagc tggtgcgctt cccgccggcc
     2941 gacgccatcc ccagcgagcg acagaggcag gtgacgcgga agctgttcgg gcacctggag
     3001 cgcggggtgc tgctgcacag cagccggcag ggcgtgttcg tcaagcggct gtgccagggc
     3061 cgcgtgttct gcagcggcaa cgccgtggtg tgcaaaggca ggcccaacaa gctggagcgt
     3121 gatgaggtgg tccaggtctt cgacaccagc cagttcttcc gagagctgca gcagttctat
     3181 aacagccagg gccggcttcc tgacggcagg gtggtgctgt gctttgggga agagtttccg
     3241 gatatggccc ccttgcgctc caaactcatt ctcgtgcaga ttgagcagct gtatgtccgg
     3301 caactggcag aagaggctgg gaagagctgt ggagccggct ctgtgatgca ggcccccgag
     3361 gagccgccgc cagaccaggt cttccggatg tttccagata tttgtgcctc acaccagaga
     3421 tcatttttca gagaaaacca acagatcacc gtcgctagcg gcagcggcgc caccaacttc
     3481 agcctgctga agcaggccgg cgacgtggag gagaaccccg gccccatgat tgaacaagat
     3541 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca
     3601 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg
     3661 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg
     3721 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact
     3781 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct
     3841 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg
     3901 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt
     3961 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc
     4021 gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc
     4081 gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga
     4141 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc
     4201 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt
     4261 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga
     4321 acgcgttaag tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     4381 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     4441 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     4501 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     4561 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     4621 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     4681 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc
     4741 tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     4801 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     4861 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     4921 ccgcgtcgac tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     4981 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg
     5041 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     5101 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     5161 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     5221 tctctagcag tacgtatagt agttcatgtc atcttattat tcagtattta taacttgcaa
     5281 agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat
     5341 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     5401 gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac
     5461 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     5521 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     5581 gtgaggaggc ttttttggag gcctagggac gtacccaatt cgccctatag tgagtcgtat
     5641 tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     5701 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
     5761 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt
     5821 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     5881 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     5941 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     6001 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     6061 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     6121 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     6181 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     6241 aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc
     6301 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     6361 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     6421 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     6481 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     6541 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     6601 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     6661 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     6721 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     6781 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     6841 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     6901 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     6961 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     7021 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     7081 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     7141 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     7201 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     7261 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     7321 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     7381 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     7441 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     7501 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     7561 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     7621 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     7681 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     7741 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     7801 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     7861 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     7921 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     7981 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     8041 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     8101 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     8161 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     8221 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     8281 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     8341 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     8401 caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac
     8461 aaaagctgga gctgcaagc
//
