LOCUS       sequence-372923-        8725 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Retroviral construct for expressing human EGFR in human cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8725)
  AUTHORS   Greulich H, Chen TH, Feng W, Janne PA, Alvarez JV, Zappaterra M, 
            Bulmer SE, Frank DA, Hahn WC, Sellers WR, Meyerson M
  TITLE     Oncogenic Transformation by Inhibitor-Sensitive and -Resistant EGFR 
            Mutants.
  JOURNAL   PLoS Med. 2005 Oct 4. 2(11):e313.
  PUBMED    16187797
REFERENCE   2  (bases 1 to 8725)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS Med. 
            2005 Oct 4. 2(11):e313."
COMMENT     Sequence Label: EGFR WT
FEATURES             Location/Qualifiers
     source          1..8725
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        473..577
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(578..646,647..1438)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1598..2186
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2087..2106
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2340..2357
                     /label=L4440
                     /note="L4440 vector, forward primer"
     LTR             2487..2956
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     CDS             complement(3177..3776)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3260..3280)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     3757..3776
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(3786..4115)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3800..3935
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4100..4120
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             complement(join(4157..5785,5786..5854,5855..7717,
                     7718..7789))
                     /codon_start=1
                     /product="precursor of human epidermal growth factor 
                     receptor"
                     /label=EGFR
                     /translation="MRPSGTAGAALLALLAALCPASRALEEKKVCQGTSNKLTQLGTFE
                     DHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENL
                     QIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQ
                     WRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGR
                     CRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVNP
                     EGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGI
                     GIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVK
                     EITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISD
                     GDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWG
                     PEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRGP
                     DNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEG
                     CPTNGPKIPSIATGMVGALLLLLVVALGIGLFMRRRHIVRKRTLRRLLQERELVEPLTP
                     SGEAPNQALLRILKETEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREATSP
                     KANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLITQLMPFGCLLDYVREHKDNIGSQ
                     YLLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGLAKLLGAEEKEYHA
                     EGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSILEKG
                     ERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIIEFSKMARDPQRYLVIQGDERMHL
                     PSPTDSNFYRALMDEEDMDDVVDADEYLIPQQGFFSSPSTSRTPLLSSLSATSNNSTVA
                     CIDRNGLQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGSVQN
                     PVYHNQPLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSHQIS
                     LDNPDYQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA"
     CDS             complement(7817..8233)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     complement(7833..7849)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(7865..7887)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     misc_feature    8434..8633
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     LTR             join(8698..8725,1..442)
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
ORIGIN
        1 tcactcagag gagaccctcc caaggaacag cgagaccaca agtcggatgc aactgcaaga
       61 gggtttattg gatacacggg tacccgggcg actcagtcaa tcggaggact ggcgccccga
      121 gtgaggggtt gtgggctctt ttattgagct cggggagcag aagcgcgcga acagaagcga
      181 gaagcgaact gattggttag ttcaaataag gcacagggtc atttcaggtc cttggggcac
      241 cctggaaaca tctgatggtt ctctagaaac tgctgagggc tggaccgcat ctggggacca
      301 tctgttcttg gccctgagcc ggggcaggaa ctgcttacca cagatatcct gtttggccca
      361 tattcagctg ttccatctgt tcttggccct gagccggggc aggaactgct taccacagat
      421 atcctgtttg gcccatattc aggctgcagg tggcactttt cggggaaatg tgcgcggaac
      481 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
      541 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
      601 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
      661 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
      721 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
      781 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
      841 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
      901 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
      961 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     1021 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     1081 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     1141 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     1201 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     1261 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     1321 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     1381 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     1441 gtcagaccaa gtttactcat atatacttta gattgatttg cggccgcaaa aggatctagg
     1501 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     1561 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     1621 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     1681 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     1741 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     1801 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     1861 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     1921 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     1981 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     2041 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     2101 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     2161 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     2221 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     2281 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     2341 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     2401 gttggccgat tcattaatgc aactatggcc atttaatgta aatacttaag aaaaaaaacc
     2461 aaattaattt tgatacatgc tgcatgtgaa agacccccgc tgacgggtag tcaatcactc
     2521 agaggagacc ctcccaagga acagcgagac cacaagtcgg atgcaactgc aagagggttt
     2581 attggataca cgggtacccg ggcgactcag tcaatcggag gactggcgcc ccgagtgagg
     2641 ggttgtgggc tcttttattg agctcgggga gcagaagcgc gcgaacagaa gcgagaagcg
     2701 aactgattgg ttagttcaaa taaggcacag ggtcatttca ggtccttggg gcaccctgga
     2761 aacatctgat ggttctctag aaactgctga gggctggacc gcatctgggg accatctgtt
     2821 cttggccctg agccggggca ggaactgctt accacagata tcctgtttgg cccatattca
     2881 gctgttccat ctgttcttgg ccctgagccg gggcaggaac tgcttaccac agatatcctg
     2941 tttggcccat attcagctgt tccatctgtt cctgaccttg atctgaactt ctctattctc
     3001 agttatgtat ttttccatgc cttgcaaaat ggcgttactt aagctagctt gccaaaccta
     3061 caggtggggt ctttcattcc cccctttttc tggagactaa ataaaatctt ttattttatc
     3121 gatgcatggg gtcgtgcgct cctttcggtc gggcgctgcg ggtcgtgggg cgggcgtcag
     3181 gcaccgggct tgcgggtcat gcaccaggtg cgcggtcctt cgggcacctc gacgtcggcg
     3241 gtgacggtga agccgagccg ctcgtagaag gggaggttgc ggggcgcgga ggtctccagg
     3301 aaggcgggca ccccggcgcg ctcggccgcc tccactccgg ggagcacgac ggcgctgccc
     3361 agacccttgc cctggtggtc gggcgagacg ccgacggtgg ccaggaacca cgcgggctcc
     3421 ttgggccggt gcggcgccag gaggccttcc atctgttgct gcgcggccag ccgggaaccg
     3481 ctcaactcgg ccatgcgcgg gccgatctcg gcgaacaccg cccccgcttc gacgctctcc
     3541 ggcgtggtcc agaccgccac cgcggcgccg tcgtccgcga cccacacctt gccgatgtcg
     3601 agcccgacgc gcgtgaggaa gagttcttgc agctcggtga cccgctcgat gtggcggtcc
     3661 ggatcgacgg tgtggcgcgt ggcggggtag tcggcgaacg cggcggcgag ggtgcgtacg
     3721 gccctgggga cgtcgtcgcg ggtggcgagg cgcaccgtgg gcttgtactc ggtcatggta
     3781 agctttttgc aaaagcctag gcctccaaaa aagcctcctc actacttctg gaatagctca
     3841 gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa ttagtcagcc atggggcgga
     3901 gaatgggcgg aactgggcgg agttaggggc gggatgggcg gagttagggg cgggactatg
     3961 gttgctgact aattgagatg catgctttgc atacttctgc ctgctgggga gcctggggac
     4021 tttccacacc tggttgctga ctaattgaga tgcatgcttt gcatacttct gcctgctggg
     4081 gagcctgggg actttccaca ccctaactga cacacattcc acagggtcga ccactgtgct
     4141 ggcgaattcc tacatctcat gctccaataa attcactgct ttgtggcgcg acccttaggt
     4201 attctgcatt ttcagctgtg gagcccttaa agatgccatt tggcttggct tccttgggaa
     4261 agaagtcctg ctggtagtca gggttgtcca ggctaatttg gtggctgcct ttctgggccc
     4321 agtgggcagg gctgtcgaat gtgctgttga cacaggtggg ctggacagtg ttgagatact
     4381 cggggttgcc cactgcagtg ctgtgggggt cctggtagtg tgggtctctg ctgggcgcgg
     4441 ggttcagagg ctgattgtga tagacaggat tctgcacaga gccagcgggc cttttgggaa
     4501 cggactggtt tatgtattca ggcactggga ggaaggtgtc gtctatgctg tcctcagtca
     4561 aggcgcctgt ggggtctgag ctgtatcgct gcaagaagct gtcttccttg atgggacagc
     4621 tttgcagccc atttctatca atgcaagcca cggtggaatt gttgctggtt gcactcagag
     4681 agctcaggag gggagtccgt gacgtggagg ggctgctgaa gaagccctgc tgtgggatga
     4741 ggtactcgtc ggcatccacc acgtcgtcca tgtcttcttc atccatcagg gcacggtaga
     4801 agttggagtc tgtaggactt ggcaaatgca ttctttcatc cccctgaatg acaaggtagc
     4861 gctgggggtc tcgggccatt ttggagaatt cgatgatcaa ctcacggaac tttgggcgac
     4921 tatctgcgtc tatcatccag cacttgacca tgatcatgta gacatcgatg gtacatatgg
     4981 gtggctgagg gaggcgttct cctttctcca ggatggagga gatctcgctg gcagggattc
     5041 cgtcatatgg cttggatcca aaggtcatca actcccaaac ggtcaccccg tagctccaga
     5101 catcactctg gtgggtatag attctgtgta aaattgattc caatgccatc cacttgatag
     5161 gcactttgcc tccttctgca tggtattctt tctcttccgc acccagcagt ttggccagcc
     5221 caaaatctgt gatcttgaca tgctgcggtg ttttcaccag tacgttcctg gctgccaggt
     5281 cgcggtgcac caagcgacgg tcctccaagt agttcatgcc ctttgcgatc tgcacacacc
     5341 agttgagcag gtactgggag ccaatattgt ctttgtgttc ccggacatag tccaggaggc
     5401 agccgaaggg catgagctgc gtgatgagtt gcacggtgga ggtgaggcag atgcccagca
     5461 ggcggcacac gtgggggttg tccacgctgg ccatcacgta ggcttcatcg aggatttcct
     5521 tgttggcttt cggagatgtt gcttctctta attccttgat agcgacggga attttaactt
     5581 tctcaccttc tgggatccag agtcccttat acaccgtgcc gaacgcaccg gagcccagca
     5641 ctttgatctt tttgaattca gtttccttca agatcctcaa gagagcttgg ttgggagctt
     5701 ctccactggg tgtaagaggc tccacaagct ccctctcctg cagcagcctc cgcagcgtgc
     5761 gcttccgaac gatgtggcgc cttcgcatga agaggccgat ccccagggcc accaccagca
     5821 gcaagaggag ggcccccacc atcccagtgg cgatggacgg gatcttaggc ccattcgttg
     5881 gacagccttc aagacctggc ccagtgcatc cgtaggtgca gtttggatgg cacaggtggc
     5941 acacatggcc ggcgtctgcg tacttccaga ccagggtgtt gttttctccc atgactcctg
     6001 ccgggcaggt cttgacgcag tgggggccgt caatgtagtg ggcacactgg atacagttgt
     6061 ctggtccccg tcctgtgcag gtgatgttca tggcctgagg caggcactct gggtggcact
     6121 gtatgcactc agagttctcc acaaactccc ttggctcacc ctccagaagg ttgcacttgt
     6181 ccacgcattc cctgcctcgg ctgacattcc ggcaagagac gcagtccctg ggctccgggc
     6241 cccagcagcc ctcgggggag cacaaggcat ggcagacctg gcctgtggcc ttgcagctgt
     6301 tttcacctct gttgcttata attttggttt tctgaccgga ggtcccaaac agttttttcc
     6361 agtttattgt atttgcatag cacaaatttt tgtttcctga aattatcaca tctccatcac
     6421 ttatctcctt gagggagcgt aatcccaagg atgttatgtt caggctgacg actgcaagag
     6481 aaaactgacc atgttgcttg gtcctgccgc gtatgatttc taggttctca aaggcatgga
     6541 ggtccgtcct gttttcaggc caagcctgaa tcagcaaaaa ccctgtgatt tcctttacgg
     6601 ttttcagaat atccagttcc tgtggatcca gaggaggagt atgtgtgaag gagtcacccc
     6661 taaatgccac cggcaggatg tggagatcgc cactgatgga ggtgcagttt ttgaagtgtt
     6721 taatattcgt agcatttatg gagagtgagt ctttaaattc accaatacct attccgttac
     6781 acactttgcg gcaaggccct tcgcacttct tacacttgcg gacgccgtct tcctccatct
     6841 catagctgtc ggccccacag gctcggacgc acgagccgtg atctgtcacc acataattac
     6901 ggggacactt cttcacgcag gtggcaccaa agctgtattt gccctcgggg ttcacatcca
     6961 tctggtacgt ggtggggttg tagagcatga gtggggggca ggtgtccttg cacgtggctt
     7021 cgtctcggaa tttgcggcag accaggcagt cgctctcccg ggggcctgtg cagcctgcag
     7081 cacactggtt gtggcagcag tcactggggg acttgccacg gcagcgcccg gagcactgct
     7141 gggcacagat gattttggtc agtttctggc agttctcctc tcctgcaccc cagcagctcc
     7201 cattgggaca gcttggatca cacttttggc agctgcccag gtggttctgg aagtccatcg
     7261 acatgttgct gagaaagtca ctgctgacta tgtcccgcca ctggatgctc tccacgttgc
     7321 acagggcagg gttgttgctg aaccgcacgg cgccatgcag gatttcctgt aaatttctca
     7381 tgggcagctc cttcagtccg gttttatttg catcatagtt agataagact gctaaggcat
     7441 aggaattttc gtagtacata tttcctctga tgatctgcag gttttccaaa ggaattcgct
     7501 ccactgtgtt gagggcaatg aggacataac cagccacctc ctggatggtc tttaagaagg
     7561 aaagatcata attcctctgc acataggtaa tttccaaatt cccaaggacc acctcacagt
     7621 tattgaacat cctctggagg ctgagaaaat gatcttcaaa agtgcccaac tgcgtgagct
     7681 tgttactcgt gccttggcaa actttctttt cctccagagc ccgactcgcc gggcagagcg
     7741 cagccagcag cgccaggagc gctgccccgg ccgtcccgga gggtcgcatg atgtaccacc
     7801 acactgggat ccggccggcg cctagagaag gagtgagggc tggataaagg gaggatcgag
     7861 gcggggtcga acgaggaggt tcaaggggga gagacggggc ggatggagga agaggaggcg
     7921 gaggcttagg gtgtacaaag ggcttgaccc agggaggggg gtcaaaagcc aaggcttccc
     7981 aggtcacgat gtaggggacc tggtctgggt gtccatgcgg gccaggtgaa aagaccttga
     8041 tcttaacctg ggtgatgagg tctcggttaa aggtgccgtc tcgcggccat ccgacgttaa
     8101 aggttggcca ttctgcagag cagaaggtaa cccaacgtct cttcttgaca tctaccgact
     8161 ggttgtgagc gatccgctcg acatctttcc agtgacctaa ggtcaaactt aagggagtgg
     8221 taacagtctg gccctaattt tcagacaaat acagaaacac agtcagacag agacaacaca
     8281 gaacgatgct gcagcgctgc agcagacaag acgcgcggct tcggttccaa accgaaagca
     8341 aaaattcaga cggaggcggg aactgtttta ggttctcgtc tcctaccaga accacatatc
     8401 ctgacggggt cggattccac atcgactccc ttcctcaggt cgggccacaa aaacggcccc
     8461 caaagtccct gggacgtctc ccagggttgc ggccgggtgt tcagaactcg tcagttccac
     8521 cacgggtccg ccagatacag agctagttag ctaactagta ccgacgcagg cgcataaaat
     8581 cagtcataga cactagacaa tcggacagac acagataagt tgctggccag cttgcctccc
     8641 ggtggtgggt cggtggtccc tgggcagggg tctcccgatc ccggacgagc ccccaaatga
     8701 aagacccccg ctgacgggta gtcaa
//
