LOCUS       sequence-372926-       10819 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GFP-tagged BRD protein, which can be used to be expressed in 
            mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10819)
  AUTHORS   Gong F, Chiu LY, Cox B, Aymard F, Clouaire T, Leung JW, Cammarata M,
            Perez M, Agarwal P, Brodbelt JS, Legube G, Miller KM
  TITLE     Screen identifies bromodomain protein ZMYND8 in chromatin 
            recognition of transcription-associated DNA damage that promotes 
            homologous recombination.
  JOURNAL   Genes Dev. 2015 Jan 15;29(2):197-211. doi: 10.1101/gad.252189.114.
  PUBMED    25593309
REFERENCE   2  (bases 1 to 10819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gad.252189"; journalName: "Genes Dev"; date: "2015-01-15- 
            15"; volume: "29"; issue: "2"; pages: "197-211"
COMMENT     Sequence Label: GFP-SMARCA4
FEATURES             Location/Qualifiers
     source          1..10819
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        147..526
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        527..730
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     681..701
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     727..751
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(855..857,858..860,861..1571)
                     /codon_start=1
                     /product="Emerald GFP"
                     /label=EmGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(900..921)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1161..1180)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1508..1529
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    1587..1611
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6556..6580)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6588..6629
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(6669..6688)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6713..6761
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6963..7391
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7050..7069)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7260..7281
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7400..7420)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7405..7734
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7585..7720
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7647..7666
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7782..7829
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7848..8246
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    8404..8525
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8441..8460)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8495..8514
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8574..8590)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8574..8590)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8587..8609)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8598..8614
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8622..8628,8629..8646,8647..8652))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8667..8688
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8805..8822)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8976..9564)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9056..9075)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9735..10526,10527..10595))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10358..10377
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10596..10700)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10775..10794)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta caacaaggca
       61 aggcttgacc gacaattgca tgaagaatct gcttagggtt aggcgttttg cgctgcttcg
      121 cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc
      181 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
      241 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
      301 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
      361 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
      421 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
      481 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
      541 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
      601 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
      661 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
      721 aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga
      781 cctccataga agacaccggg accgatccag cctccggact ctagaggatc cctaccggtg
      841 atatcctcga gaccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
      901 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
      961 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
     1021 tgccctggcc caccctcgtg accaccttca cctacggcgt gcagtgcttc gcccgctacc
     1081 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
     1141 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
     1201 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
     1261 acatcctggg gcacaagctg gagtacaact acaacagcca caaggtctat atcaccgccg
     1321 acaagcagaa gaacggcatc aaggtgaact tcaagacccg ccacaacatc gaggacggca
     1381 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
     1441 tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc
     1501 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg
     1561 agctgtacaa gggctcgagc ccatcaacaa gtttgtacaa aaaagttggc accatgtcca
     1621 ctccagaccc acccctgggc ggaactcctc ggccaggtcc ttccccgggc cctggccctt
     1681 cccctggagc catgctgggc cctagcccgg gtccctcgcc gggctccgcc cacagcatga
     1741 tggggcccag cccagggccg ccctcagcag gacaccccat ccccacccag gggcctggag
     1801 ggtaccctca ggacaacatg caccagatgc acaagcccat ggagtccatg catgagaagg
     1861 gcatgtcgga cgacccgcgc tacaaccaga tgaaaggaat ggggatgcgg tcagggggcc
     1921 atgctgggat ggggcccccg cccagcccca tggaccagca ctcccaaggt tacccctcgc
     1981 ccctgggtgg ctctgagcat gcctctagtc cagttccagc cagtggcccg tcttcggggc
     2041 cccagatgtc ttccgggcca ggaggtgccc cgctggatgg tgctgacccc caggccttgg
     2101 ggcagcagaa ccggggccca accccattta accagaacca gctgcaccag ctcagagctc
     2161 agatcatggc ctacaagatg ctggccaggg ggcagcccct ccccgaccac ctgcagatgg
     2221 cggtgcaggg caagcggccg atgcccggga tgcagcagca gatgccaacg ctacctccac
     2281 cctcggtgtc cgcaacagga cccggccctg gccctggccc tggccccggc ccgggtcccg
     2341 gcccggcacc tccaaattac agcaggcctc atggtatggg agggcccaac atgcctcccc
     2401 caggaccctc gggcgtgccc cccgggatgc caggccagcc tcctggaggg cctcccaagc
     2461 cctggcctga aggacccatg gcgaatgctg ctgcccccac gagcacccct cagaagctga
     2521 ttcccccgca gccaacgggc cgcccttccc ccgcgccccc tgccgtccca cccgccgcct
     2581 cgcccgtgat gccaccgcag acccagtccc ccgggcagcc ggcccagccc gcgcccatgg
     2641 tgccactgca ccagaagcag agccgcatca cccccatcca gaagccgcgg ggcctcgacc
     2701 ctgtggagat cctgcaggag cgcgagtaca ggctgcaggc tcgcatcgca caccgaattc
     2761 aggaacttga aaaccttccc gggtccctgg ccggggattt gcgaaccaaa gcgaccattg
     2821 agctcaaggc cctcaggctg ctgaacttcc agaggcagct gcgccaggag gtggtggtgt
     2881 gcatgcggag ggacacagcg ctggagacag ccctcaatgc taaggcctac aagcgcagca
     2941 agcgccagtc cctgcgcgag gcccgcatca ctgagaagct ggagaagcag cagaagatcg
     3001 agcaggagcg caagcgccgg cagaagcacc aggaatacct caatagcatt ctccagcatg
     3061 ccaaggattt caaggaatat cacagatccg tcacaggcaa aatccagaag ctgaccaagg
     3121 cagtggccac gtaccatgcc aacacggagc gggagcagaa gaaagagaac gagcggatcg
     3181 agaaggagcg catgcggagg ctcatggctg aagatgagga ggggtaccgc aagctcatcg
     3241 accagaagaa ggacaagcgc ctggcctacc tcttgcagca gacagacgag tacgtggcta
     3301 acctcacgga gctggtgcgg cagcacaagg ctgcccaggt cgccaaggag aaaaagaaga
     3361 aaaagaaaaa gaagaaggca gaaaatgcag aaggacagac gcctgccatt gggccggatg
     3421 gcgagcctct ggacgagacc agccagatga gcgacctccc ggtgaaggtg atccacgtgg
     3481 agagtgggaa gatcctcaca ggcacagatg cccccaaagc cgggcagctg gaggcctggc
     3541 tcgagatgaa cccggggtat gaagtagctc cgaggtctga tagtgaagaa agtggctcag
     3601 aagaagagga agaggaggag gaggaagagc agccgcaggc agcacagcct cccaccctgc
     3661 ccgtggagga gaagaagaag attccagatc cagacagcga tgacgtctct gaggtggacg
     3721 cgcggcacat cattgagaat gccaagcaag atgtcgatga tgaatatggc gtgtcccagg
     3781 cccttgcacg tggcctgcag tcctactatg ccgtggccca tgctgtcact gagagagtgg
     3841 acaagcagtc agcgcttatg gtcaatggtg tcctcaaaca gtaccagatc aaaggtttgg
     3901 agtggctggt gtccctgtac aacaacaacc tgaacggcat cctggccgac gagatgggcc
     3961 tggggaagac catccagacc atcgcgctca tcacgtacct catggagcac aaacgcatca
     4021 atgggccctt cctcatcatc gtgcctctct caacgctgtc caactgggcg tacgagtttg
     4081 acaagtgggc cccctccgtg gtgaaggtgt cttacaaggg atccccagca gcaagacggg
     4141 cctttgtccc ccagctccgg agtgggaagt tcaacgtctt gctgacgacg tacgagtaca
     4201 tcatcaaaga caagcacatc ctcgccaaga tccgttggaa gtacatgatt gtggacgaag
     4261 gtcaccgcat gaagaaccac cactgcaagc tgacgcaggt gctcaacacg cactatgtgg
     4321 caccccgccg cctgctgctg acgggcacac cgctgcagaa caagcttccc gagctctggg
     4381 cgctgctcaa cttcctgctg cccaccatct tcaagagctg cagcaccttc gagcagtggt
     4441 ttaacgcacc ctttgccatg accggggaaa aggtggacct gaatgaggag gaaaccattc
     4501 tcatcatccg gcgtctccac aaagtgctgc ggcccttctt gctccgacga ctcaagaagg
     4561 aagtcgaggc ccagttgccc gaaaaggtgg agtacgtcat caagtgcgac atgtctgcgc
     4621 tgcagcgagt gctctaccgc cacatgcagg ccaagggcgt gctgctgact gatggctccg
     4681 agaaggacaa gaagggcaaa ggcggcacca agaccctgat gaacaccatc atgcagctgc
     4741 ggaagatctg caaccacccc tacatgttcc agcacatcga ggagtccttt tccgagcact
     4801 tggggttcac tggcggcatt gtccaagggc tggacctgta ccgagcctcg ggtaaatttg
     4861 agcttcttga tagaattctt cccaaactcc gagcaaccaa ccacaaagtg ctgctgttct
     4921 gccaaatgac ctccctcatg accatcatgg aagattactt tgcgtatcgc ggctttaaat
     4981 acctcaggct tgatggaacc acgaaggcgg aggaccgggg catgctgctg aaaaccttca
     5041 acgagcccgg ctctgagtac ttcatcttcc tgctcagcac ccgggctggg gggctcggcc
     5101 tgaacctcca gtcggcagac actgtgatca tttttgacag cgactggaat cctcaccagg
     5161 acctgcaagc gcaggaccga gcccaccgca tcgggcagca gaacgaggtg cgtgtgctcc
     5221 gcctctgcac cgtcaacagc gtggaggaga agatcctagc tgcagccaag tacaagctca
     5281 acgtggacca gaaggtgatc caggccggca tgttcgacca gaagtcctcc agccatgagc
     5341 ggcgcgcctt cctgcaggcc atcctggagc acgaggagca ggatgagagc agacactgca
     5401 gcacgggcag cggcagtgcc agcttcgccc acactgcccc tccgccagcg ggcgtcaacc
     5461 ccgacttgga ggagccacct ctaaaggagg aagacgaggt gcccgacgac gagaccgtca
     5521 accagatgat cgcccggcac gaggaggagt ttgatctgtt catgcgcatg gacctggacc
     5581 gcaggcgcga ggaggcccgc aaccccaagc ggaagccgcg cctcatggag gaggacgagc
     5641 tcccctcgtg gatcatcaag gacgacgcgg aggtggagcg gctgacctgt gaggaggagg
     5701 aggagaagat gttcggccgt ggctcccgcc accgcaagga ggtggactac agcgactcac
     5761 tgacggagaa gcagtggctc aaggccatcg aggagggcac gctggaggag atcgaagagg
     5821 aggtccggca gaagaaatca tcacggaagc gcaagcgaga cagcgacgcc ggctcctcca
     5881 ccccgaccac cagcacccgc agccgcgaca aggacgacga gagcaagaag cagaagaagc
     5941 gcgggcggcc gcctgccgag aaactctccc ctaacccacc caacctcacc aagaagatga
     6001 agaagattgt ggatgccgtg atcaagtaca aggacagcag cagtggacgt cagctcagcg
     6061 aggtcttcat ccagctgccc tcgcgaaagg agctgcccga gtactacgag ctcatccgca
     6121 agcccgtgga cttcaagaag ataaaggagc gcattcgcaa ccacaagtac cgcagcctca
     6181 acgacctaga gaaggacgtc atgctcctgt gccagaacgc acagaccttc aacctggagg
     6241 gctccctgat ctatgaagac tccatcgtct tgcagtcggt cttcaccagc gtgcggcaga
     6301 aaatcgagaa ggaggatgac agtgaaggcg aggagagtga ggaggaggaa gagggcgagg
     6361 aggaaggctc cgaatccgaa tctcggtccg tcaaagtgaa gatcaagctt ggccggaagg
     6421 agaaggcaca ggaccggctg aagggcggcc ggcggcggcc gagccgaggg tcccgagcca
     6481 agccggtcgt gagtgacgat gacagtgagg aggaacaaga ggaggaccgc tcaggaagtg
     6541 gcagcgaaga agacttgcca actttcttgt acaaagtggt tgataacggg aagcctatcc
     6601 ctaaccctct cctcggtctc gattctacgc gtaccggtta gtaatgagtt taaacggggg
     6661 aggctaactg aaacacggaa ggagacaata ccggaaggaa cccgcgctat gacggcaata
     6721 aaaagacaga ataaaacgca cgggtgttgg gtcgtttgtt cataaacgcg gggttcggtc
     6781 ccagggctgg cactctgtcg ataccccacc gagaccccat tggggccaat acgcccgcgt
     6841 ttcttccttt tccccacccc accccccaag ttcgggtgaa ggcccagggc tcgcagccaa
     6901 cgtcggggcg gcaggccctg ccatagcaga tctgcgcagc tggggctcta gggggtatcc
     6961 ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     7021 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     7081 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     7141 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
     7201 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     7261 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
     7321 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     7381 taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc
     7441 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag
     7501 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     7561 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     7621 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct
     7681 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc
     7741 ccgggagctt gtatatccat tttcggatct gatcagcacg tgttgacaat taatcatcgg
     7801 catagtatat cggcatagta taatacgaca aggtgaggaa ctaaaccatg gccaagcctt
     7861 tgtctcaaga agaatccacc ctcattgaaa gagcaacggc tacaatcaac agcatcccca
     7921 tctctgaaga ctacagcgtc gccagcgcag ctctctctag cgacggccgc atcttcactg
     7981 gtgtcaatgt atatcatttt actgggggac cttgtgcaga actcgtggtg ctgggcactg
     8041 ctgctgctgc ggcagctggc aacctgactt gtatcgtcgc gatcggaaat gagaacaggg
     8101 gcatcttgag cccctgcgga cggtgccgac aggtgcttct cgatctgcat cctgggatca
     8161 aagccatagt gaaggacagt gatggacagc cgacggcagt tgggattcgt gaattgctgc
     8221 cctctggtta tgtgtgggag ggctaagcac ttcgtggccg aggagcagga ctgacacgtg
     8281 ctacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc
     8341 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac
     8401 cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     8461 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     8521 tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag
     8581 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     8641 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     8701 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     8761 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     8821 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     8881 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     8941 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     9001 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     9061 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     9121 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     9181 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     9241 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     9301 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     9361 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     9421 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     9481 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     9541 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     9601 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     9661 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     9721 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     9781 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     9841 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     9901 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     9961 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
    10021 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
    10081 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
    10141 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
    10201 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
    10261 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
    10321 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
    10381 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
    10441 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
    10501 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
    10561 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
    10621 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
    10681 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg
    10741 ggagatctcc cgatccccta tggtgcactc tcagtacaat ctgctctgat gccgcatagt
    10801 taagccagta tctgctccc
//
