LOCUS       sequence-372935-        9450 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  3rd gen lentiviral V5-Luciferase expression vector, PGK promoter, 
            Blast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9450)
  AUTHORS   Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, 
            Campisi J, Yaswen P, Cooper PK, Kaufman PD.
  TITLE     A versatile viral system for expression and depletion of proteins in
            mammalian cells.
  JOURNAL   PLoS One. 2009 Aug 6;4(8):e6529.
  PUBMED    19657394
REFERENCE   2  (bases 1 to 9450)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-08-6"; volume: "4(8)"; pages: "e6529"
COMMENT     Sequence Label: pLenti PGK Blast V5-LUC (w528-1)
FEATURES             Location/Qualifiers
     source          1..9450
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      6..141
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     306..326
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     misc_feature    377..965
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             483..494
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     892..912
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     protein_bind    996..1020
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(1027..2679)
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     2595..2613
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     regulatory      2676..2685
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(2755..2796)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    complement(2808..2832)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(2887..3397)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2950..2968)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     misc_feature    3443..3560
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(3827..3871)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4056..4289
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    4782..4907
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             4954..5134
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(5135..5361)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(5389..5409)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5389..5407)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5428..5444)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5428..5444)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5441..5463)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5452..5468
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5476..5482,5483..5500,5501..5506))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5521..5542
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5659..5676)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5830..6418)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5910..5929)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6589..7380,7381..7449))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7212..7231
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7450..7554)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7580..8035)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7717..7738)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7929..7948
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8162..8184
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8176..8193
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8177..8193
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8203..8222
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8203..8221
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      8242..8377
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    8417..8538
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8428..8447)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8482..8501
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             8610..8843
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     CDS             complement(8930..9328)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     promoter        complement(9347..9394)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(join(9442..9450,1..321))
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
ORIGIN
        1 gcctaggcct ccaaaaaagc ctcctcacta cttctggaat agctcagagg cagaggcggc
       61 ctcggcctct gcataaataa aaaaaattag tcagccatgg ggcggagaat gggcggaact
      121 gggcggagtt aggggcggga tgggcggagt taggggcggg actatggttg ctgactaatt
      181 gagatgcatg ctttgcatac ttctgcctgc tggggagcct ggggactttc cacacctggt
      241 tgctgactaa ttgagatgca tgctttgcat acttctgcct gctggggagc ctggggactt
      301 tccacaccct aactgacaca cattccacag aattaattcc aaactcatta ctaaccggat
      361 atctgcagaa ttccaggcgg ggaggcggcc caaagggaga tccgactcgt ctgagggcga
      421 aggcgaagac gcggaagagg ccgcagagcc ggcagcaggc cgcgggaagg aaggtccgct
      481 ggattgaggg ccgaagggac gtagcagaag gacgtcccgc gcagaatcca ggtggcaaca
      541 caggcgagca gccatggaaa ggacgtcagc ttccccgaca acaccacgga attgtcagtg
      601 cccaacagcc gagcccctgt ccagcagcgg gcaaggcagg cggcgatgag ttccgccgtg
      661 gcaataggga gggggaaagc gaaagtcccg gaaaggagct gacaggtggt ggcaatgccc
      721 caaccagtgg gggttgcgtc agcaaacaca gtgcacacca cgccacgttg cctgacaacg
      781 ggccacaact cctcataaag agacagcaac caggatttat acaaggagga gaaaatgaaa
      841 gccatacggg aagcaatagc atgatacaaa ggcattaaag cagcgtatcc acatagcgta
      901 aaaggagcaa catagttaag aataccagtc aatctttcac aaattttgta atccagaggt
      961 tgattgtcga gcggccgcca ctgtgctgga tatcaaccac tttgtacaag aaagctgggt
     1021 ctagaattac acggcgatct ttccgccctt cttggccttt atgaggatct ctctgatttt
     1081 tcttgcgtcg agttttccgg taagaccttt cggtacttcg tccacaaaca caactcctcc
     1141 gcgcaacttt ttcgcggttg ttacttgact ggcgacgtaa tccacgatct ctttttccgt
     1201 catcgtcttt ccgtgctcca aaacaacaac ggcggcggga agttcaccgg cgtcatcgtc
     1261 gggaagacct gcgacacctg cgtcgaagat gttggggtgt tggagcaaga tggattccaa
     1321 ttcagcggga gccacctgat agcctttgta cttaatcaga gacttcaggc ggtcaacgat
     1381 gaagaagtgt tcgtcttcgt cccagtaagc tatgtctcca gaatgtagcc atccatcctt
     1441 gtcaatcaag gcgttggtcg cttccggatt gtttacataa ccggacataa tcataggacc
     1501 tctcacacac agttcgcctc tttgattaac gcccagcgtt ttcccggtat ccagatccac
     1561 aaccttcgct tcaaaaaatg gaacaacttt accgaccgcg cccggtttat catccccctc
     1621 gggtgtaatc agaatagctg atgtagtctc agtgagccca tatccttgcc tgatacctgg
     1681 cagatggaac ctcttggcaa ccgcttcccc gacttcctta gagaggggag cgccaccaga
     1741 agcaatttcg tgtaaattag ataaatcgta tttgtcaatc agagtgcttt tggcgaagaa
     1801 ggagaatagg gttggcacca gcagcgcact ttgaatcttg taatcctgaa ggctcctcag
     1861 aaacagctct tcttcaaatc tatacattaa gacgactcga aatccacata tcaaatatcc
     1921 gagtgtagta aacattccaa aaccgtgatg gaatggaaca acacttaaaa tcgcagtatc
     1981 cggaatgatt tgattgccaa aaataggatc tctggcatgc gagaatctca cgcaggcagt
     2041 tctatgaggc agagcgacac ctttaggcag accagtagat ccagaggagt tcatgatcag
     2101 tgcaattgtc ttgtccctat cgaaggactc tggcacaaaa tcgtattcat taaaaccggg
     2161 aggtagatga gatgtgacga acgtgtacat cgactgaaat ccctggtaat ccgttttaga
     2221 atccatgata ataatttttt ggatgattgg gagctttttt tgcacgttca aaattttttg
     2281 caaccccttt ttggaaacga acaccacggt aggctgcgaa atgcccatac tgttgagcaa
     2341 ttcacgttca ttataaatgt cgttcgcggg cgcaactgca actccgataa ataacgcgcc
     2401 caacaccggc ataaagaatt gaagagagtt ttcactgcat acgacgattc tgtgatttgt
     2461 attcagccca tatcgtttca tagcttctgc caaccgaacg gacatttcga agtactcagc
     2521 gtaagtgatg tccacctcga tatgtgcatc tgtaaaagca attgttccag gaaccagggc
     2581 gtatctcttc atagccttat gcagttgctc tccagcggtt ccatcttcca gcggatagaa
     2641 tggcgccggg cctttcttta tgtttttggc gtcttccatg gtggctttac caacagtacc
     2701 ggaatgccaa gctttttgca aaagcctagg gactgaattg gttcccatgg atcccgtaga
     2761 atcgagaccg aggagagggt tagggatagg cttaccttcc atggtggagc ctgctttttt
     2821 gtacaaactt gttgatatct gcagaattcc accacactgg actagtcgac gaaccggtgg
     2881 atccccctgg ggagagaggt cggtgattcg gtcaacgagg gagccgactg ccgacgtgcg
     2941 ctccggaggc ttgcagaatg cggaacaccg cgcgggcagg aacagggccc acactaccgc
     3001 cccacacccc gcctcccgca ccgccccttc ccggccgctg ctctcggcgc gccctgctga
     3061 gcagccgcta ttggccacag cccatcgcgg tcggcgcgct gccattgctc cctggcgctg
     3121 tccgtctgcg agggtactag tgagacgtgc ggcttccgtt tgtcacgtcc ggcacgccgc
     3181 gaaccgcaag gaaccttccc gacttagggg cggagcagga agcgtcgccg gggggcccac
     3241 aagggtagcg gcgaagatcc gggtgacgct gcgaacggac gtgaagaatg tgcgagaccc
     3301 agggtcggcg ccgctgcgtt tcccggaacc acgcccagag cagccgcgtc cctgcgcaaa
     3361 cccagggctg ccttggaaaa ggcgcaaccc caaccccgtg gaattcgata tcaagcttct
     3421 cgaggctagt ctcgtgatcg ataaaatttt gaatttttgt aatttgtttt tgtaattctt
     3481 tagtttgtat gtctgttgct attatgtcta ctattctttc ccctgcactg taccccccaa
     3541 tccccccttt tcttttaaaa gttaaccgat accgtcgaga tccgttcact aatcgaatgg
     3601 atctgtctct gtctctctct ccaccttctt cttctattcc ttcgggcctg tcgggtcccc
     3661 tcggggttgg gaggtgggtc tgaaacgata atggtgaata tccctgccta actctattca
     3721 ctatagaaag tacagcaaaa actattctta aacctaccaa gcctcctact atcattatga
     3781 ataattttat ataccacagc caatttgtta tgttaaacca attccacaaa cttgcccatt
     3841 tatctaattc caataattct tgttcattct tttcttgctg gttttgcgat tcttcaatta
     3901 aggagtgtat taagcttgtg taattgttaa tttctctgtc ccactccatc caggtcgtgt
     3961 gattccaaat ctgttccaga gatttattac tccaactagc attccaaggc acagcagtgg
     4021 tgcaaatgag ttttccagag caaccccaaa tccccaggag ctgttgatcc tttaggtatc
     4081 tttccacagc caggattctt gcctggagct gcttgatgcc ccagactgtg agttgcaaca
     4141 gatgctgttg cgcctcaata gccctcagca aattgttctg ctgctgcact ataccagaca
     4201 ataattgtct ggcctgtacc gtcagcgtca ttgacgctgc gcccatagtg cttcctgctg
     4261 ctcccaagaa cccaaggaac aaagctccta ttcccactgc tcttttttct ctctgcacca
     4321 ctcttctctt tgccttggtg ggtgctactc ctaatggttc aatttttact actttatatt
     4381 tatataattc acttctccaa ttgtccctca tatctcctcc tccaggtctg aagatcagcg
     4441 gccgcttgct gtgcggtggt cttacttttg ttttgctctt cctctatctt gtctaaagct
     4501 tccttggtgt cttttatctc tatcctttga tgcacacaat agagggttgc tactgtatta
     4561 tataatgatc taagttcttc tgatcctgtc tgaagggatg gttgtagctg tcccagtatt
     4621 tgtctacagc cttctgatgt ttctaacagg ccaggattaa ctgcgaatcg ttctagctcc
     4681 ctgcttgccc atactatatg ttttaattta tattttttct ttccccctgg ccttaaccga
     4741 attttttccc atcgcgatct aattctcccc cgcttaatac tgacgctctc gcacccatct
     4801 ctctccttct agcctccgct agtcaaaatt tttggcgtac tcaccagtcg ccgcccctcg
     4861 cctcttgccg tgcgcgcttc agcaagccga gtcctgcgtc gagagagctc ctctggtttc
     4921 cctttcgctt tcaagtccct gttcgggcgc cactgctaga gattttccac actgactaaa
     4981 agggtctgag ggatctctag ttaccagagt cacacaacag acgggcacac actacttgaa
     5041 gcactcaagg caagctttat tgaggcttaa gcagtgggtt ccctagttag ccagagagct
     5101 cccaggctca gatctggtct aaccagagag acccgtttat gtatcgagct aggcacttaa
     5161 atacaatatc tctgcaatgc ggcaattcag tggttcgtcc aatccatgtc agacccgtct
     5221 gttgccttcc taataaggca cgatcgtacc accttacttc caccaatcgg catgcacggt
     5281 gctttttctc tccttgtaag gcatgttgct aactcatcgt taccatgttg caagactaca
     5341 agagtattgc ataagactac attaagcttg cagctccagc ttttgttccc tttagtgagg
     5401 gttaattgcg cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     5461 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     5521 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     5581 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     5641 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     5701 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     5761 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     5821 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     5881 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     5941 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     6001 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     6061 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     6121 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     6181 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     6241 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     6301 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     6361 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     6421 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     6481 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     6541 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     6601 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     6661 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     6721 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     6781 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     6841 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     6901 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     6961 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     7021 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     7081 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     7141 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     7201 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     7261 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     7321 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     7381 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     7441 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     7501 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     7561 tccccgaaaa gtgccaccta aattgtaagc gttaatattt tgttaaaatt cgcgttaaat
     7621 ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa
     7681 tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta
     7741 ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca
     7801 ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat
     7861 cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg
     7921 agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc
     7981 acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtcccat
     8041 tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta
     8101 cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt
     8161 tcccagtcac gacgttgtaa aacgacggcc agtgagcgcg cgtaatacga ctcactatag
     8221 ggcgaattgg gtacgtccct aggcctccaa aaaagcctcc tcactacttc tggaatagct
     8281 cagaggccga ggcggcctcg gcctctgcat aaataaaaaa aattagtcag ccatggggcg
     8341 gagaatgggc ggaactgggc ggagttaggg gcgggatggg cggagttagg ggcgggatag
     8401 ctagagccag acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag
     8461 tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata
     8521 agctgcaata aacaagttcc tctcactctc tgatattcat ttctttgcaa gttataaata
     8581 ctgaataata agatgacatg aactactact gctagagatt ttccacactg actaaaaggg
     8641 tctgagggat ctctagttac cagagtcaca caacagacgg gcacacacta cttgaagcac
     8701 tcaaggcaag ctttattgag gcttaagcag tgggttccct agttagccag agagctccca
     8761 ggctcagatc tggtctaacc agagagaccc agtacaagca aaaagcagat cttgtcttcg
     8821 ttgggagtga gctagccctt ccagtccccc cttttctttt aaaaagtggc taagatctac
     8881 agctgccttg taagtcattg gtcttaaagg taccgagctc gaattgtgct tagccctccc
     8941 acacataacc agagggcagc aattcacgaa tcccaactgc cgtcggctgt ccatcactgt
     9001 ccttcactat ggctttgatc ccaggatgca gatcgagaag cacctgtcgg caccgtccgc
     9061 aggggctcaa gatgcccctg ttctcatttc cgatcgcgac gatacaagtc aggttgccag
     9121 ctgccgcagc agcagcagtg cccagcacca cgagttctgc acaaggtccc ccagtaaaat
     9181 gatatacatt gacaccagtg aagatgcggc cgtcgctaga gagagctgcg ctggcgacgc
     9241 tgtagtcttc agagatgggg atgctgttga ttgtagccgt tgctctttca atgagggtgg
     9301 attcttcttg agacaaaggc ttggccatgg tttagttcct caccttgtcg tattatacta
     9361 tgccgatata ctatgccgat gattaattgt caacacgtgc tgatcagatc cgaaaatgga
     9421 tatacaagct cccgggagct ttttgcaaaa
//
