LOCUS       sequence-372938-        4942 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4942)
  AUTHORS   Korchynskyi O, ten Dijke P
  TITLE     Identification and functional characterization of distinct 
            critically important bone morphogenetic protein-specific response 
            elements in the Id1 promoter.
  JOURNAL   J Biol Chem. 2002 Feb 15;277(7):4883-91. Epub 2001 Nov 29.
  PUBMED    11729207
REFERENCE   2  (bases 1 to 4942)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2002-02-15"; volume: "277(7)"; pages: "4883-91. Epub 
            2001 Nov 29"
COMMENT     Sequence Label: pGL3 BRE Luciferase
FEATURES             Location/Qualifiers
     source          1..4942
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    94..185
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(126..145)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    199..247
                     /note="synthetic polyadenylation signal"
     rep_origin      complement(378..833)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(515..536)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     727..746
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        860..964
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(965..1033,1034..1825)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1183..1202)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1996..2584
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2485..2504
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2738..2755
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    3003..3124
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3040..3059)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3094..3113
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(3165..4817)
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     4733..4751
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     regulatory      4814..4823
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gctgtcaggc tggcgccgcg gcgccagcct gacagcccgt cctggcgtct aacggtctga
       61 gctagcacgc gtaagagctc ggtacctatc gatagagaaa tgttctggca cctgcacttg
      121 cactggggac agcctatttt gctagtttgt tttgtttcgt tttgttttga tggagagcgt
      181 atgttagtac tatcgattca cacaaaaaac caacacacag atgtaatgaa aataaagata
      241 ttttattgcg gccgctccaa gtacctcccg taccttaata ttacttactt atcatggtag
      301 cttgggctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag
      361 cctgaatggc gaatggcaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt
      421 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc
      481 aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt
      541 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact
      601 acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg
      661 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag
      721 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac
      781 gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcaggtgg
      841 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
      901 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
      961 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     1021 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     1081 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     1141 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     1201 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     1261 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     1321 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     1381 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     1441 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     1501 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     1561 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     1621 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     1681 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     1741 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     1801 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     1861 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     1921 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     1981 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     2041 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     2101 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     2161 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     2221 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     2281 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     2341 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     2401 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     2461 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     2521 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     2581 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     2641 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     2701 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     2761 ggaagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg
     2821 cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca
     2881 agggcatcgg tcgacggatc cttatcgatt ttaccacatt tgtagaggtt ttacttgctt
     2941 taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg
     3001 ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     3061 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     3121 cttatcatgt ctgctcgaag cggccggccg ccccgactct agaattacac ggcgatcttt
     3181 ccgcccttct tggcctttat gaggatctct ctgatttttc ttgcgtcgag ttttccggta
     3241 agacctttcg gtacttcgtc cacaaacaca actcctccgc gcaacttttt cgcggttgtt
     3301 acttgactgg cgacgtaatc cacgatctct ttttccgtca tcgtctttcc gtgctccaaa
     3361 acaacaacgg cggcgggaag ttcaccggcg tcatcgtcgg gaagacctgc gacacctgcg
     3421 tcgaagatgt tggggtgttg gagcaagatg gattccaatt cagcgggagc cacctgatag
     3481 cctttgtact taatcagaga cttcaggcgg tcaacgatga agaagtgttc gtcttcgtcc
     3541 cagtaagcta tgtctccaga atgtagccat ccatccttgt caatcaaggc gttggtcgct
     3601 tccggattgt ttacataacc ggacataatc ataggacctc tcacacacag ttcgcctctt
     3661 tgattaacgc ccagcgtttt cccggtatcc agatccacaa ccttcgcttc aaaaaatgga
     3721 acaactttac cgaccgcgcc cggtttatca tccccctcgg gtgtaatcag aatagctgat
     3781 gtagtctcag tgagcccata tccttgcctg atacctggca gatggaacct cttggcaacc
     3841 gcttccccga cttccttaga gaggggagcg ccaccagaag caatttcgtg taaattagat
     3901 aaatcgtatt tgtcaatcag agtgcttttg gcgaagaagg agaatagggt tggcaccagc
     3961 agcgcacttt gaatcttgta atcctgaagg ctcctcagaa acagctcttc ttcaaatcta
     4021 tacattaaga cgactcgaaa tccacatatc aaatatccga gtgtagtaaa cattccaaaa
     4081 ccgtgatgga atggaacaac acttaaaatc gcagtatccg gaatgatttg attgccaaaa
     4141 ataggatctc tggcatgcga gaatctcacg caggcagttc tatgaggcag agcgacacct
     4201 ttaggcagac cagtagatcc agaggagttc atgatcagtg caattgtctt gtccctatcg
     4261 aaggactctg gcacaaaatc gtattcatta aaaccgggag gtagatgaga tgtgacgaac
     4321 gtgtacatcg actgaaatcc ctggtaatcc gttttagaat ccatgataat aattttttgg
     4381 atgattggga gctttttttg cacgttcaaa attttttgca accccttttt ggaaacgaac
     4441 accacggtag gctgcgaaat gcccatactg ttgagcaatt cacgttcatt ataaatgtcg
     4501 ttcgcgggcg caactgcaac tccgataaat aacgcgccca acaccggcat aaagaattga
     4561 agagagtttt cactgcatac gacgattctg tgatttgtat tcagcccata tcgtttcata
     4621 gcttctgcca accgaacgga catttcgaag tactcagcgt aagtgatgtc cacctcgata
     4681 tgtgcatctg taaaagcaat tgttccagga accagggcgt atctcttcat agccttatgc
     4741 agttgctctc cagcggttcc atcttccagc ggatagaatg gcgccgggcc tttctttatg
     4801 tttttggcgt cttccatggt ggctttacca acagtaccgg aatgccaagc ttggaagaga
     4861 gtgaggacga acgcgccccc accccctttt atagcccaga tctcgagccc gggctagctc
     4921 agaccgttag acgccaggac gg
//
