LOCUS       sequence-37294-e         760 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 760)
  AUTHORS   Luker KE, Smith MC, Luker GD, Gammon ST, Piwnica-Worms H, 
            Piwnica-Worms D
  TITLE     Kinetics of regulated protein-protein interactions revealed with 
            firefly luciferase complementation imaging in cells and living 
            animals.
  JOURNAL   Proc Natl Acad Sci U S A. 2004 Aug 17. 101(33):12288-93.
  PUBMED    15284440
REFERENCE   2  (bases 1 to 760)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2004 Aug 17. 101(33):12288-93."
COMMENT     Sequence Label: CLuc-FKBP
FEATURES             Location/Qualifiers
     source          1..760
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(1..42)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             complement(131..454)
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
ORIGIN
        1 cgtagaatcg agaccgagga gagggttagg gataggctta ccttcgaacc gcgggccctc
       61 tagactcgag cggccgccac tgtgctggat atctgcagaa ttgcccttct gcagaagctt
      121 actaactagt tcattccagt tttagaagct ccacatcgaa gacgagagtg gcatgtggtg
      181 ggatgatgcc tgggtgccca gtggcaccat aggcataatc tggagatata gtcagtttgg
      241 ctctctgacc cacactcatc tgggcaaccc cttcttccca gcctcggatc acctcctgct
      301 tgcctagcat aaacttaaag ggcttgtttc tgtcccggga ggaatcaaat ttctttccat
      361 cttcaagcat cccggtgtag tgcaccacgc aggtctggcc gcgcttgggg aaggtgcgcc
      421 cgtctcctgg ggagatggtt tccacctgca ctccgccccg ggacgcgtac gagatctgac
      481 ctccgccaga tgagccacct cccacggcga tctttccgcc cttcttggcc tttatgagga
      541 tctctctgat ttttcttgcg tcgagttttc cggtaagacc tttcggtact tcgtccacaa
      601 acacaactcc tccgcgcaac tttttcgcgg ttgttacttg actggcgacg taatccacga
      661 tctctttttc cgtcatcgtc tttccgtgct ccaaaacaac aacggcggcg ggaagttcac
      721 cggcgtcatc gtcgggaaga cctgcgacac ctgcgtcgaa
//
