LOCUS       sequence-372942-        5757 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Luciferase reporter containing three copies of vitellogenin Estrogen
            Response Element .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5757)
  AUTHORS   Hall JM, McDonnell DP
  TITLE     The estrogen receptor beta-isoform (ERbeta) of the human estrogen 
            receptor modulates ERalpha transcriptional activity and is a key 
            regulator of the cellular response to estrogens and antiestrogens.
  JOURNAL   Endocrinology. 1999 Dec . 140(12):5566-78.
  PUBMED    10579320
REFERENCE   2  (bases 1 to 5757)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Endocrinology. 1999 Dec . 140(12):5566-78."
COMMENT     Sequence Label: 3X ERE TATA luc
FEATURES             Location/Qualifiers
     source          1..5757
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     61..79
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     polyA_signal    405..526
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(716..1171)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(853..874)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1065..1084
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        1198..1302
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1303..1371,1372..2163)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1521..1540)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2334..2922
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2823..2842
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3076..3093
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    3235..3369
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(3794..3814)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          3944..4009
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             complement(join(4250..5757,1..145))
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
ORIGIN
        1 cctcgatatg tgcatctgta aaagcaattg ttccaggaac cagggcgtat ctcttcatag
       61 ccttatgcag ttgctctcca gcggttccat cctctagagg atagaatggc gccgggcctt
      121 tctttatgtt tttggcgtct tccattttac caacagtacc ggaatgccaa gcttgcatgc
      181 ctgcaggtcg actctagagg atccgtctcc agaatgaggg ccgagctccc cccaccccct
      241 tttatagccc gaattcatcg atgatatcag atctaggtca cagtgacctg cggatctagg
      301 tcacagtgac ctgcggatcc gcaggtcact gtgacctaga tctcgagcta gcacgcgtaa
      361 gagctcggta cctcccgggt tatgttagct cagttacagt accataagat acattgatga
      421 gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga
      481 tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg
      541 cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa
      601 cctccacgta ccttaatatt acttacttat catggtagct tgggctggcg taatagcgaa
      661 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcaaatt
      721 gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt
      781 aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg
      841 ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc
      901 aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca
      961 agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga
     1021 tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa
     1081 ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc
     1141 gccgcgctta atgcgccgct acagggcgcg tcaggtggca cttttcgggg aaatgtgcgc
     1201 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     1261 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     1321 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     1381 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     1441 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     1501 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     1561 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     1621 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     1681 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     1741 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     1801 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     1861 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     1921 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     1981 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     2041 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     2101 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     2161 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     2221 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     2281 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     2341 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     2401 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     2461 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     2521 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     2581 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     2641 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     2701 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     2761 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     2821 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     2881 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     2941 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     3001 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     3061 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgctg ccggcacctg
     3121 tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg
     3181 cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacggatcca
     3241 gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa
     3301 tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat
     3361 aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg
     3421 gaggtttttt aaagcaagta aaacctctac aaatgtggta tggctgatta tgatctctag
     3481 tcaaggcact atacatcaaa tattccttat taaccccttt acaaattaaa aagctaaagg
     3541 tacacaattt ttgagcatag ttattaatag cagacactct atgcctgtgt ggagtaagaa
     3601 aaaacagtat gttatgatta taactgttat gcctacttat aaaggttaca gaatattttt
     3661 ccataatttt cttgtatagc agtgcagctt tttcctttgt ggtgtaaata gcaaagcaag
     3721 caagagttct attactaaac acagcatgac tcaaaaaact tagcaattct gaaggaaagt
     3781 ccttggggtc ttctaccttt ctcttctttt ttggaggagt agaatgttga gagtcagcag
     3841 tagcctcatc atcactagat ggcatttctt ctgagcaaaa caggttttcc tcattaaagg
     3901 cattccacca ctgctcccat tcatcagttc cataggttgg aatctaaaat acacaaacaa
     3961 ttagaatcag tagtttaaca cattatacac ttaaaaattt tatatttacc ttagagcttt
     4021 aaatctctgt aggtagtttg tccaattatg tcacaccaca gaagtaaggt tccttcacaa
     4081 agatccggcg aatttctgcc attcatccgc ttattatcac ttattcaggc gtagcaacca
     4141 ggcgtttaag ggcaccaata actgccttaa aaaaattacg ccccgccctg ccactcatcg
     4201 cagtattaca atagctaaga atttcgtcat cgctgaatac agttacattt tacaatttgg
     4261 actttccgcc cttcttggcc tttatgagga tctctctgat ttttcttgcg tcgagttttc
     4321 cggtaagacc tttcggtact tcgtccacaa acacaactcc tccgcgcaac tttttcgcgg
     4381 ttgttacttg actggcgacg taatccacga tctctttttc cgtcatcgtc tttccgtgct
     4441 ccaaaacaac aacggcggcg ggaagttcac cggcgtcatc gtcgggaaga cctgccacgc
     4501 ccgcgtcgaa gatgttgggg tgttgtaaca atatcgattc caattcagcg ggggccacct
     4561 gatatccttt gtatttaatt aaagacttca agcggtcaac tatgaagaag tgttcgtctt
     4621 cgtcccagta agctatgtct ccagaatgta gccatccatc cttgtcaatc aaggcgttgg
     4681 tcgcttccgg attgtttaca taaccggaca taatcatagg tcctctgaca cataattcgc
     4741 ctctctgatt aacgcccagc gttttcccgg tatccagatc cacaaccttc gcttcaaaaa
     4801 atggaacaac tttaccgacc gcgcccggtt tatcatcccc ctcgggtgta atcagaatag
     4861 ctgatgtagt ctcagtgagc ccatatcctt gtcgtatccc tggaagatgg aagcgttttg
     4921 caaccgcttc cccgacttct ttcgaaagag gtgcgccccc agaagcaatt tcgtgtaaat
     4981 tagataaatc gtatttgtca atcagagtgc ttttggcgaa gaatgaaaat agggttggta
     5041 ctagcaacgc actttgaatt ttgtaatcct gaagggatcg taaaaacagc tcttcttcaa
     5101 atctatacat taagacgact cgaaatccac atatcaaata tccgagtgta gtaaacattc
     5161 caaaaccgtg atggaatgga acaacactta aaatcgcagt atccggaatg atttgattgc
     5221 caaaaatagg atctctggca tgcgagaatc tgacgcaggc agttctatgc ggaagggcca
     5281 cacccttagg taacccagta gatccagagg aattcattat cagtgcaatt gttttgtcac
     5341 gatcaaagga ctctggtaca aaatcgtatt cattaaaacc gggaggtaga tgagatgtga
     5401 cgaacgtgta catcgactga aatccctggt aatccgtttt agaatccatg ataataattt
     5461 tctggattat tggtaatttt ttttgcacgt tcaaaatttt ttgcaacccc tttttggaaa
     5521 caaacactac ggtaggctgc gaaatgttca tactgttgag caattcacgt tcattataaa
     5581 tgtcgttcgc gggcgcaact gcaactccga taaataacgc gcccaacacc ggcataaaga
     5641 attgaagaga gttttcactg catacgacga ttctgtgatt tgtattcagc ccatatcgtt
     5701 tcatagcttc tgccaaccga acggacattt cgaagtattc cgcgtacgtg atgttca
//
