LOCUS       sequence-372944-       10409 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Contains Golden Gate cassette to express up to four gRNAs and dsRED.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10409)
  AUTHORS   Kabadi AM, Ousterout DG, Hilton IB, Gersbach CA
  TITLE     Multiplex CRISPR/Cas9-based genome engineering from a single 
            lentiviral vector.
  JOURNAL   Nucleic Acids Res. 2014 Aug 13. pii: gku749.
  PUBMED    25122746
REFERENCE   2  (bases 1 to 10409)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2014 Aug 13. pii: gku749."
COMMENT     Sequence Label: pLV GG hUbC-dsRED
FEATURES             Location/Qualifiers
     source          1..10409
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     2..23
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(142..162)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        147..476
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      327..462
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     389..408
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        524..571
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             590..964
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    1094..1215
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1131..1150)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1185..1204
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1264..1280)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1288..1304
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1312..1318,1319..1336,1337..1342))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1357..1378
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1495..1512)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1666..2254)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1746..1765)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2425..3216,3217..3285))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3048..3067
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3286..3390)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3465..3484)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3656..4035
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4037..4235
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4190..4210
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             4253..4433
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4480..4605
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5098..5331
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5516..5560
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5858..5975
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    complement(6020..6053)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(6040..6366)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSAQLTLTKGNKSWRTSRGGPVPNSPYSESYYARSLAVVL
                     QRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGATTVSY
                     HNFV"
     primer_bind     6216..6238
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6230..6247
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6231..6247
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6257..6276
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6257..6275
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6323..6343)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6323..6341)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6362..6378)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6386..6402
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6410..6416,6417..6434,6435..6440))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6455..6476
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        6553..7764
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     7601..7620
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      7811..7820
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7817..8494
                     /codon_start=1
                     /product="noncytotoxic tetrameric variant of DsRed 
                     fluorescent protein (Strack et al., 2008)"
                     /label=DsRed-Express2
                     /note="mammalian codon-optimized"
                     /translation="MDSTENVIKPFMRFKVHMEGSVNGHEFEIEGEGEGKPYEGTQTAK
                     LQVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGTFIYHVKFIGVNFPSDGPVMQKKTLGWEPSTERLYPRDGVLKGEIHK
                     ALKLKGGGHYLVEFKSIYMAKKPVKLPGYYYVDSKLDITSHNEDYTVVEQYERAEARHH
                     LFQ"
     primer_bind     complement(7997..8017)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     8215..8234
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     8409..8432
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     misc_feature    8519..9107
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8572..8592)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8990..9001)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(9110..9126)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9111..9127)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             9632..9812
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9838..9855)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9844..10068
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      join(10114..10409,1..133)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10201..10220)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
       61 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
      121 tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct
      181 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa
      241 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
      301 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
      361 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct
      421 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc
      481 tcccgggagc ttgtatatcc attttcggat ctgatcagca cgtgttgaca attaatcatc
      541 ggcatagtat atcggcatag tataatacga caaggtgagg aactaaacca tggccaagtt
      601 gaccagtgcc gttccggtgc tcaccgcgcg cgacgtcgcc ggagcggtcg agttctggac
      661 cgaccggctc gggttctccc gggacttcgt ggaggacgac ttcgccggtg tggtccggga
      721 cgacgtgacc ctgttcatca gcgcggtcca ggaccaggtg gtgccggaca acaccctggc
      781 ctgggtgtgg gtgcgcggcc tggacgagct gtacgccgag tggtcggagg tcgtgtccac
      841 gaacttccgg gacgcctccg ggccggccat gaccgagatc ggcgagcagc cgtgggggcg
      901 ggagttcgcc ctgcgcgacc cggccggcaa ctgcgtgcac ttcgtggccg aggagcagga
      961 ctgacacgtg ctacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg
     1021 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt
     1081 cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     1141 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     1201 catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc
     1261 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     1321 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     1381 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     1441 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     1501 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     1561 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     1621 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     1681 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     1741 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     1801 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     1861 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     1921 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     1981 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     2041 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     2101 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     2161 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     2221 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     2281 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     2341 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     2401 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     2461 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     2521 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     2581 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     2641 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     2701 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     2761 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     2821 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     2881 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     2941 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     3001 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     3061 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     3121 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     3181 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     3241 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     3301 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     3361 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
     3421 cgacggatcg ggagatctcc cgatccccta tggtgcactc tcagtacaat ctgctctgat
     3481 gccgcatagt taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc
     3541 gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg acaattgcat gaagaatctg
     3601 cttagggtta ggcgttttgc gctgcttcgc gatgtacggg ccagatatac gcgttgacat
     3661 tgattattga ctagttatta atagtaatca attacggggt cattagttca tagcccatat
     3721 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
     3781 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
     3841 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
     3901 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
     3961 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
     4021 atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt
     4081 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac
     4141 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc
     4201 ggtaggcgtg tacggtggga ggtctatata agcagcgcgt tttgcctgta ctgggtctct
     4261 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     4321 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     4381 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
     4441 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
     4501 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
     4561 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
     4621 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
     4681 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
     4741 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
     4801 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
     4861 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
     4921 agtaagacca ccgcacagca agcggccgct gatcttcaga cctggaggag gagatatgag
     4981 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     5041 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     5101 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
     5161 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     5221 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     5281 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     5341 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     5401 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
     5461 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     5521 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     5581 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     5641 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     5701 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga
     5761 aggtggagag agagacagag acagatccat tcgattagtg aacggatcgg cactgcgtgc
     5821 gccaattctg cagacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg
     5881 gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag
     5941 aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag
     6001 atccagtttg gttaattaaa taacttcgta tagcatacat tatacgaagt tatgataaga
     6061 gacggtggtt gcgccgctac agggcgcgtc ccattcgcca ttcaggctgc gcaactgttg
     6121 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc
     6181 tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac
     6241 ggccagtgag cgcgcgtaat acgactcact atagggcgaa ttgggtaccg ggccccccct
     6301 cgaggtcctc cagcttttgt tccctttagt gagggttaat tgcgcgcttg gcgtaatcat
     6361 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     6421 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     6481 cgttgcgctc actgcccgct ttccactgca tgacgtctcc acaattaatt aacccgtgtc
     6541 ggctccagat ctggcctccg cgccgggttt tggcgcctcc cgcgggcgcc cccctcctca
     6601 cggcgagcgc tgccacgtca gacgaagggc gcagcgagcg tcctgatcct tccgcccgga
     6661 cgctcaggac agcggcccgc tgctcataag actcggcctt agaaccccag tatcagcaga
     6721 aggacatttt aggacgggac ttgggtgact ctagggcact ggttttcttt ccagagagcg
     6781 gaacaggcga ggaaaagtag tcccttctcg gcgattctgc ggagggatct ccgtggggcg
     6841 gtgaacgccg atgattatat aaggacgcgc cgggtgtggc acagctagtt ccgtcgcagc
     6901 cgggatttgg gtcgcggttc ttgtttgtgg atcgctgtga tcgtcacttg gtgagtagcg
     6961 ggctgctggg ctggccgggg ctttcgtggc cgccgggccg ctcggtggga cggaagcgtg
     7021 tggagagacc gccaagggct gtagtctggg tccgcgagca aggttgccct gaactggggg
     7081 ttggggggag cgcagcaaaa tggcggctgt tcccgagtct tgaatggaag acgcttgtga
     7141 ggcgggctgt gaggtcgttg aaacaaggtg gggggcatgg tgggcggcaa gaacccaagg
     7201 tcttgaggcc ttcgctaatg cgggaaagct cttattcggg tgagatgggc tggggcacca
     7261 tctggggacc ctgacgtgaa gtttgtcact gactggagaa ctcggtttgt cgtctgttgc
     7321 gggggcggca gttatggcgg tgccgttggg cagtgcaccc gtacctttgg gagcgcgcgc
     7381 cctcgtcgtg tcgtgacgtc acccgttctg ttggcttata atgcagggtg gggccacctg
     7441 ccggtaggtg tgcggtaggc ttttctccgt cgcaggacgc agggttcggg cctagggtag
     7501 gctctcctga atcgacaggc gccggacctc tggtgagggg agggataagt gaggcgtcag
     7561 tttctttggt cggttttatg tacctatctt cttaagtagc tgaagctccg gttttgaact
     7621 atgcgctcgg ggttggcgag tgtgttttgt gaagtttttt aggcaccttt tgaaatgtaa
     7681 tcatttgggt caatatgtaa ttttcagtgt tagactagta aattgtccgc taaattctgg
     7741 ccgtttttgg cttttttgtt agacgaagct tgggctgcag gtcgactcta gaggatcccc
     7801 gggtaccggt gccaccatgg atagcactga gaacgtcatc aagcccttca tgcgcttcaa
     7861 ggtgcacatg gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg
     7921 caagccctac gagggcaccc agaccgccaa gctgcaggtg accaagggcg gccccctgcc
     7981 cttcgcctgg gacatcctgt ccccccagtt ccagtacggc tccaaggtgt acgtgaagca
     8041 ccccgccgac atccccgact acaagaagct gtccttcccc gagggcttca agtgggagcg
     8101 cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga
     8161 cggcaccttc atctaccacg tgaagttcat cggcgtgaac ttcccctccg acggccccgt
     8221 aatgcagaag aagactctgg gctgggagcc ctccaccgag cgcctgtacc cccgcgacgg
     8281 cgtgctgaag ggcgagatcc acaaggcgct gaagctgaag ggcggcggcc actacctggt
     8341 ggagttcaag tcaatctaca tggccaagaa gcccgtgaag ctgcccggct actactacgt
     8401 ggactccaag ctggacatca cctcccacaa cgaggactac accgtggtgg agcagtacga
     8461 gcgcgccgag gcccgccacc acctgttcca gtaggaattc gatatcaagc ttatcgataa
     8521 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     8581 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     8641 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     8701 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     8761 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     8821 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     8881 gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc
     8941 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     9001 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
     9061 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcatc gataccgtcg
     9121 acctcgagac ctagaaaaac atggagcaat cacaagtagc aatacagcag ctaccaatgc
     9181 tgattgtgcc tggctagaag cacaagagga ggaggaggtg ggttttccag tcacacctca
     9241 ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
     9301 aaagggggga ctggaagggc taattcactc ccaacgaaga caagatatcc ttgatctgtg
     9361 gatctaccac acacaaggct acttccctga ttggcagaac tacacaccag ggccagggat
     9421 cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc aagagaaggt
     9481 agaagaagcc aatgaaggag agaacacccg cttgttacac cctgtgagcc tgcatgggat
     9541 ggatgacccg gagagagaag tattagagtg gaggtttgac agccgcctag catttcatca
     9601 catggcccga gagctgcatc cggactgtac tgggtctctc tggttagacc agatctgagc
     9661 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     9721 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     9781 acccttttag tcagtgtgga aaatctctag cagggcccgt ttaaacccgc tgatcagcct
     9841 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
     9901 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt
     9961 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg
    10021 attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg
    10081 aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg
    10141 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
    10201 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
    10261 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
    10321 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
    10381 ctttgacgtt ggagtccacg ttctttaat
//
