LOCUS       sequence-372947-        4947 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Monomeric near-infrared fluorescent protein miRFP709 in bacterial 
            plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4947)
  AUTHORS   Shcherbakova DM, Baloban M, Emelyanov AV, Brenowitz M, Guo P, 
            Verkhusha VV
  TITLE     Bright monomeric near-infrared fluorescent proteins as tags and 
            biosensors for multiscale imaging.
  JOURNAL   Nat Commun. 2016 Aug 19;7:12405. doi: 10.1038/ncomms12405.
  PUBMED    27539380
REFERENCE   2  (bases 1 to 4947)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-08-19"; volume: "7:12405. doi"; pages: " 
            10.1038/ncomms12405"
COMMENT     Sequence Label: pBAD/His-miRFP709
FEATURES             Location/Qualifiers
     source          1..4947
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(3..30)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(122..208)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     361..378
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     CDS             complement(424..1371)
                     /codon_start=1
                     /product="bright monomeric near-infrared fluorescent 
                     protein engineered from a bacterial phytochrome, with an 
                     emission maximum of 709 nm (Shcherbakova et al., 2016)"
                     /label=miRFP709
                     /label=ORF frame 1
                     /translation="MVAGHASGSPAFGTASHSNCEHEEIHLAGSIQPHGALLVVSEHDH
                     RVIQASANAAEFLNLGSVLGVPLAEIDGDLLIKILPHLDPTAEGMPVAVRCRIGNPSTE
                     YCGLMHRPPEGGLIIELERAGPSIDLSGTLAPALERIRTAGSLRALCDDTVLLFQQCTG
                     YDRVMVYRFDEQGHGLVFSECHVPGLESYFGNRYPSSFIPQMARQLYVRQRVRVLVDVT
                     YQPVPLEPRLSPLTGRDLDMSGCFLRSMSPIHLQFLKDMGVRATLAVSLVVGGKLWGLV
                     VCHHYLPRFIRFELRAICKRLAERIATRITALES"
     CDS             complement(1381..1398)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        complement(1441..1725)
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     complement(1499..1518)
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     CDS             1752..2630
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     primer_bind     complement(2786..2808)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    2894..3034
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     2949..2968
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(3049..3066)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3220..3808)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3300..3319)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3979..4770,4771..4839))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4602..4621
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4840..4931)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 caaaaaggcc atccgtcagg atggccttct gcttaatttg atgcctggca gtttatggcg
       61 ggcgtcctgc ccgccaccct ccgggccgtt gcttcgcaac gttcaaatcc gctcccggcg
      121 gatttgtcct actcaggaga gcgttcaccg acaaacaaca gataaaacga aaggcccagt
      181 ctttcgactg agcctttcgt tttatttgat gcctggcagt tccctactct cgcatgggga
      241 gaccccacac taccatcggc gctacggcgt ttcacttctg agttcggcat ggggtcaggt
      301 gggaccaccg cgctactgcc gccaggcaaa ttctgtttta tcagaccgct tctgcgttct
      361 gatttaatct gtatcaggct gaaaatcttc tctcatccac caaaacagcc aagcttcgaa
      421 ttcttagctc tcaagcgcgg tgatccgcgt cgcgatcctt tcggcgagcc gtttgcagat
      481 cgcccgcagc tcgaaacgga tgaagcgcgg cagataatgg tgacagacaa ccaggcccca
      541 cagcttgccg ccgaccacca gcgacaccgc cagggtggcg cgcacgccca tgtccttcag
      601 gaactgcaga tggatcggcg acatcgagcg caggaagcag cccgacatgt cgagatcgcg
      661 cccggtcagc ggcgacagcc gcggctccag cggcaccggc tgataggtga cgtcgaccag
      721 cacgcggacg cgctgccgca cgtacagctg ccgcgccatc tgcgggatga acgacgacgg
      781 atagcggttg ccgaaatagg attcgagccc aggcacatgg cactcggaga ataccaggcc
      841 gtggccttgc tcatcgaaac gatacaccat cacccggtcg tagccggtgc actgctgaaa
      901 cagcagcacg gtgtcatcgc acagcgcgcg cagtgaaccc gccgtgcgga tccgctccag
      961 cgccggcgcc agcgtgcctg acagatcgat cgacgggccg gcacgttcga gttcgatgat
     1021 cagcccgcct tccggaggcc gatgcatcag accgcagtac tccgtagagg gattgccgat
     1081 ccggcagcgc accgcgaccg gcatgccttc ggcggtggga tcgagatgcg gcaggatctt
     1141 gatcaacaga tcgccgtcga tctcggcgag cggaacgccg agtacgcttc cgagattcag
     1201 aaattccgcg gcgttggcgc tggcctggat gacgcgatga tcatgttcgc tgacgaccag
     1261 aagcgcgcca tgcggctgga tcgagccggc gaggtggatc tcttcatgtt cgcaattcga
     1321 atgagaggcg gtcccgaatg cggggctgcc agaggcatga cctgctacca tagatctgcc
     1381 atggtgatgg tgatggtggc tccccatggt taattcctcc tgttagccca aaaaacgggt
     1441 atggagaaac agtagagagt tgcgataaaa agcgtcaggt aggatccgct aatcttatgg
     1501 ataaaaatgc tatggcatag caaagtgtga cgccgtgcaa ataatcaatg tggacttttc
     1561 tgccgtgatt atagacactt ttgttacgcg tttttgtcat ggctttggtc ccgctttgtt
     1621 acagaatgct tttaataagc ggggttaccg gtttggttag cgagaagagc cagtaaaaga
     1681 cgcagtgacg gcaatgtctg atgcaatatg gacaattggt ttcttctctg aatggcggga
     1741 gtatgaaaag tatggctgaa gcgcaaaatg atcccctgct gccgggatac tcgtttaatg
     1801 cccatctggt ggcgggttta acgccgattg aggccaacgg ttatctcgat ttttttatcg
     1861 accgaccgct gggaatgaaa ggttatattc tcaatctcac cattcgcggt cagggggtgg
     1921 tgaaaaatca gggacgagaa tttgtttgcc gaccgggtga tattttgctg ttcccgccag
     1981 gagagattca tcactacggt cgtcatccgg aggctcgcga atggtatcac cagtgggttt
     2041 actttcgtcc gcgcgcctac tggcatgaat ggcttaactg gccgtcaata tttgccaata
     2101 cggggttctt tcgcccggat gaagcgcacc agccgcattt cagcgacctg tttgggcaaa
     2161 tcattaacgc cgggcaaggg gaagggcgct attcggagct gctggcgata aatctgcttg
     2221 agcaattgtt actgcggcgc atggaagcga ttaacgagtc gctccatcca ccgatggata
     2281 atcgggtacg cgaggcttgt cagtacatca gcgatcacct ggcagacagc aattttgata
     2341 tcgccagcgt cgcacagcat gtttgcttgt cgccgtcgcg tctgtcacat cttttccgcc
     2401 agcagttagg gattagcgtc ttaagctggc gcgaggacca acgtatcagc caggcgaagc
     2461 tgcttttgag caccacccgg atgcctatcg ccaccgtcgg tcgcaatgtt ggttttgacg
     2521 atcaactcta tttctcgcgg gtatttaaaa aatgcaccgg ggccagcccg agcgagttcc
     2581 gtgccggttg tgaagaaaaa gtgaatgatg tagccgtcaa gttgtcataa ttggtaacga
     2641 atcagacaat tgacggcttg acggagtagc atagggtttg cagaatccct gcttcgtcca
     2701 tttgacaggc acattatgca tgccgcttcg ccttcgcgcg cgaattgatc tgctgcctcg
     2761 cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag
     2821 cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg
     2881 gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga tagcggagtg tatactggct
     2941 taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc
     3001 gcacagatgc gtaaggagaa aataccgcat caggcgctct tccgcttcct cgctcactga
     3061 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     3121 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     3181 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     3241 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     3301 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     3361 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     3421 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     3481 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     3541 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     3601 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     3661 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     3721 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     3781 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     3841 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     3901 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     3961 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     4021 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     4081 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     4141 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     4201 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     4261 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     4321 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     4381 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     4441 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     4501 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     4561 tatgcggcga ccgagttgct cttgcccggc gtcaacacgg gataataccg cgccacatag
     4621 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     4681 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     4741 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     4801 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     4861 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     4921 aaataaacaa aagagtttgt agaaacg
//
