LOCUS       sequence-372962-        7468 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  NOTE: An improved version of this plasmid is now available. See 
            Addgene plasmid #232199..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7468)
  TITLE     AAV Packaging
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7468)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAAV2/2
FEATURES             Location/Qualifiers
     source          1..7468
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(170..758)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(250..269)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(929..1720,1721..1789))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1552..1571
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1790..1894)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      1921..2376
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2008..2027)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2217..2238
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2502..2524
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2516..2533
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2517..2533
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(3008..3019)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     protein_bind    complement(7109..7156)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     complement(7170..7186)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7170..7186)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(7236..7252)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7236..7252)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7249..7271)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7260..7276
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7284..7290,7291..7308,7309..7314))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7329..7350
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(join(7467..7468,1..16))
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
       61 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
      121 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
      181 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
      241 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
      301 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
      361 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
      421 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
      481 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
      541 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
      601 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
      661 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
      721 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
      781 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
      841 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
      901 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
      961 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     1021 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     1081 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     1141 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     1201 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     1261 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     1321 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     1381 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     1441 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     1501 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     1561 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     1621 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     1681 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     1741 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     1801 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     1861 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     1921 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     1981 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     2041 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     2101 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     2161 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc ttaatagtgg
     2221 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
     2281 agggattttg ccgatttcgg cctattggtt aaaaaaatga gctgatttaa caaaaattta
     2341 acgcgaattt taacaaaata ttaacgctta caatttccat tcgccattca ggctgcgcaa
     2401 ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg
     2461 atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa
     2521 aacgacggcc agtgagcgcg ccagcgctcg ttcaaacctc ccgctcaaaa tggagaccct
     2581 gcgtgctcac tcgggcttaa atacccagcg tgaccacatg gtgtcgcaaa atgtcgcaaa
     2641 acactcacgt gacctctaat acaggactct ccctaaccct atgacgtaat tcacgtcacg
     2701 actccacccc tccgcccgct gtttaaacgc ccgggctgta gttaatgatt aacccgccat
     2761 gctacttatc tacgtagcca tggaaactag ataagaaaga aatacgcaga gaccaaagtt
     2821 caactgaaac gaattaaacg gtttattgat taacaagcaa ttacagatta cgagtcaggt
     2881 atctggtgcc aatggggcga ggctctgaat acacgccatt agtgtccaca gtaaagtcca
     2941 cattaacaga cttgttgtag ttggaagtgt actgaatttc gggattccag cgtttgctgt
     3001 tttccttctg cagctcccac tcgatctcca cgctgacctg tcccgtggag tactgtgtga
     3061 tgaaggaagc aaactttgcc gcactgaagg tggtcgaagg attcgcaggt accggggtgt
     3121 tcttgatgag aatctgtgga ggagggtgtt taagtccgaa tccacccatg aggggagagg
     3181 ggtgaaaatg tccgtccgtg tgtggaatct ttgcccagat gggcccctga aggtacacat
     3241 ctctgtcctg ccagaccatg cctggaagaa cgccttgtgt gttgacatct gcggtagctg
     3301 cttgtctgtt gcctctctgg aggttggtag atacagaacc atactgctcc gtagccacgg
     3361 gattggttgt cctgatttcc tcttcgtctg taatcatgac cttttcaatg tccacatttg
     3421 ttttctctga gccttgcttc ccaaagatga gaaccccgct ctgaggaaaa aacttttctt
     3481 catcgtcctt gtggcttgcc atggccgggc ccggattcac cagagagtct ctgccattga
     3541 ggtggtactt ggtagctcca gtccacgagt attcactgtt gttgttatcc gcagatgtct
     3601 ttgatactcg ctgctggcgg taacagggtc caggaagcca gttcctagac tggtcccgaa
     3661 tgtcactcgc tccggcctga gaaaactgaa gccttgactg cgtggtggtt ccacttggag
     3721 tgtttgttct gctcaagtaa tacaggtact ggtcgatgag aggattcatg agacggtcca
     3781 gactctggct gtgagcgtag ctgctgtgga aaggaacgtc ctcaaaagtg tagctgaagg
     3841 taaagttgtt tccggtacgc agcatctgag aaggaaagta ctccaggcag taaaatgaag
     3901 agcgtcctac tgcctgactc ccgttgttca gggtgaggta tccatactgt ggcaccatga
     3961 agacgtctgc tgggaacggc gggaggcatc cttgatgcgc cgagccgagg acgtacggga
     4021 gctggtactc cgagtcagta aacacctgaa ccgtgctggt aaggttattg gcaatcgtcg
     4081 tcgtaccgtc attctgcgtg acctctttga cttgaatgtt aaagagcttg aagttgagtc
     4141 tcttgggtcg gaatccccag ttgttgttga tgagtctttg ccagtcacgt ggtgaaaagt
     4201 ggcagtggaa tctgttgaag tcaaaatacc cccaaggggt gctgtagcca aagtagtgat
     4261 tgtcgttcga ggctcctgat tggctggaaa tttgtttgta gaggtggttg ttgtaggtgg
     4321 gcagggccca ggttcgggtg ctggtggtga tgactctgtc gcccatccat gtggaatcgc
     4381 aatgccaatt tcccgaggaa ttacccactc cgtcggcgcc ctcgttattg tctgccattg
     4441 gtgcgccact gcctgtagcc atcgtattag ttcccagacc agagggggct gctggtggct
     4501 gtccgagagg ctgggggtca ggtactgagt ctgcgtctcc agtctgacca aaattcaatc
     4561 tttttcttgc aggctgctgg cccgcctttc cggttcccga ggaggagtct ggctccacag
     4621 gagagtgctc taccggcctc ttttttcccg gagccgtctt aacaggttcc tcaaccaggc
     4681 ccagaggttc aagaaccctc tttttcgcct ggaagactgc tcgtccgagg ttgcccccaa
     4741 aagacgtatc ttctttaagg cgctcctgaa actccgcgtc ggcgtggttg tacttgaggt
     4801 acgggttgtc tccgctgtcg agctgccggt cgtaggcttt gtcgtgctcg agggccgcgg
     4861 cgtctgcctc gttgaccggc tctcccttgt cgagtccgtt gaagggtccg aggtacttgt
     4921 acccaggaag cacaagaccc ctgctgtcgt ccttatgccg ctctgcgggc tttggtggtg
     4981 gtgggccagg tttgagcttc caccactgtc ttattccttc agagagagtg tcctcgagcc
     5041 aatctggaag ataaccatcg gcagccatac ctgatttaaa tcatttattg ttcaaagatg
     5101 cagtcatcca aatccacatt gaccagatcg caggcagtgc aagcgtctgg cacctttccc
     5161 atgatatgat gaatgtagca cagtttctga tacgcctttt tgacgacaga aacgggttga
     5221 gattctgaca cgggaaagca ctctaaacag tctttctgtc cgtgagtgaa gcagatattt
     5281 gaattctgat tcattctctc gcattgtctg cagggaaaca gcatcagatt catgcccacg
     5341 tgacgagaac atttgttttg gtacctgtct gcgtagttga tcgaagcttc cgcgtctgac
     5401 gtcgatggct gcgcaactga ctcgcgcacc cgtttgggct cacttatatc tgcgtcactg
     5461 ggggcgggtc ttttcttggc tccacccttt ttgacgtaga attcatgctc cacctcaacc
     5521 acgtgatcct ttgcccaccg gaaaaagtct ttgacttcct gcttggtgac cttcccaaag
     5581 tcatgatcca gacggcgggt gagttcaaat ttgaacatcc ggtcttgcaa cggctgctgg
     5641 tgttcgaagg tcgttgagtt cccgtcaatc acggcgcaca tgttggtgtt ggaggtgacg
     5701 atcacgggag tcgggtctat ctgggccgag gacttgcatt tctggtccac gcgcaccttg
     5761 cttcctccga gaatggcttt ggccgactcc acgaccttgg cggtcatctt cccctcctcc
     5821 caccagatca ccatcttgtc gacacagtcg ttgaagggaa agttctcatt ggtccagttt
     5881 acgcacccgt agaagggcac agtgtgggct atggcctccg cgatgttggt cttcccggta
     5941 gttgcaggcc caaacagcca gatggtgttc ctcttgccga actttttcgt ggcccatccc
     6001 agaaagacgg aagccgcata ttggggatcg tacccgttta gttccaaaat tttataaatc
     6061 cgattgctgg aaatgtcctc cacgggctgc tggcccacca ggtagtcggg ggcggtttta
     6121 gtcaggctca taatctttcc cgcattgtcc aaggcagcct tgatttggga ccgcgagttg
     6181 gaggccgcat tgaaggagat gtatgaggcc tggtcctcct ggatccactg cttctccgag
     6241 gtaatcccct tgtccacgag ccacccgacc agctccatgt acctggctga agtttttgat
     6301 ctgatcaccg gcgcatcaga attgggattc tgattctctt tgttctgctc ctgcgtctgc
     6361 gacacgtgcg tcagatgctg cgccaccaac cgtttacgct ccgtgagatt caaacaggcg
     6421 cttaaatact gttccatatt agtccacgcc cactggagct caggctgggt tttggggagc
     6481 aagtaattgg ggatgtagca ctcatccacc accttgttcc cgcctccggc gccatttctg
     6541 gtctttgtga ccgcgaacca gtttggcaaa gtcggctcga tcccgcggta aattctctga
     6601 atcagttttt cgcgaatctg actcaggaaa cgtcccaaaa ccatggattt caccccggtg
     6661 gtttccacga gcacgtgcat gtggaagtag ctctctccct tctcaaattg cacaaagaaa
     6721 agggcctccg gggccttact cacacggcgc cattccgtca gaaagtcgcg ctgcagcttc
     6781 tcggccacgg tcaggggtgc ctgctcaatc agattcagat ccatgtcaga atctggcggc
     6841 aactcccatt ccttctcggc cacccagttc acaaagctgt cagaaatgcc gggcagatgc
     6901 tcgtcaaggt cgctggggac cttaatcaca atctcgtaaa accccggcat ggcggctgcg
     6961 cgttcaaacc tcccgcttca aaatggagac cctgcgtgct cactcgggct taaataccca
     7021 gcgtgaccac atggtgtcgc aaaatgtcgc aaaacactca cgtgacctct aatacaggac
     7081 tctaggggta ccgagctcga atagatcaga agttcctata ctttctagag aataggaact
     7141 tcggaatagg aacttctgat cttccggggg atccactagt tctagagcgg ccggcgcggg
     7201 gcgttaacga tatcggcgcg cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     7261 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     7321 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     7381 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     7441 ttgcgtattg ggcgctcttc cgcttcct
//
