LOCUS       sequence-372968-        6024 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  In vivo visualization of microtubules (can be used for 
            colocalization studies).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6024)
  AUTHORS   Bindels DS, Haarbosch L, van Weeren L, Postma M, Wiese KE, Mastop M,
            Aumonier S, Gotthard G, Royant A, Hink MA, Gadella TW Jr
  TITLE     mScarlet: a bright monomeric red fluorescent protein for cellular 
            imaging.
  JOURNAL   Nat Methods. 2016 Nov 21. doi: 10.1038/nmeth.4074.
  PUBMED    27869816
REFERENCE   2  (bases 1 to 6024)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Nov 21. doi: 10.1038/nmeth.4074."
COMMENT     Sequence Label: pmScarlet_alphaTubulin_C1
FEATURES             Location/Qualifiers
     source          1..6024
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      12..147
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        164..355
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     312..332
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        complement(357..461)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      488..943
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(575..594)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     785..806
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    950..1071
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(961..980)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1015..1034
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(2575..3270)
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     primer_bind     complement(2850..2869)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     3119..3137
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      3267..3276
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(3315..3518)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(3344..3364)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        3519..3822
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     rep_origin      complement(3997..4585)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4077..4096)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    4914..4961
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     4986..5005
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(5193..5987)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(5193..5987)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5304..5323)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     5914..5933
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(join(6022..6024,1..355))
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
ORIGIN
        1 gcaaaagcct aggcctccaa aaaagcctcc tcactacttc tggaatagct cagaggccga
       61 ggcggcctcg gcctctgcat aaataaaaaa aattagtcag ccatggggcg gagaatgggc
      121 ggaactgggc ggagttaggg gcgggatggg cggagttagg ggcgggacta tggttgctga
      181 ctaattgaga tgcatgcttt gcatacttct gcctgctggg gagcctgggg actttccaca
      241 cctggttgct gactaattga gatgcatgct ttgcatactt ctgcctgctg gggagcctgg
      301 ggactttcca caccctaact gacacacatt ccacagctgg ttctttccgc ctcaggactc
      361 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
      421 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
      481 ccacctgacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
      541 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
      601 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
      661 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
      721 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
      781 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
      841 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
      901 aaatttaacg cgaattttaa caaaatatta acgcttacaa tttacgcgtt aagatacatt
      961 gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt
     1021 tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac
     1081 aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag
     1141 taaaacctct acaaatgtgg tatggctgat tatgatcagt tatctagatc cggtggatcc
     1201 ttagtattcc tctccttctt cctcaccctc tccttcaaca gaatccacac caacctcctc
     1261 ataatccttc tcaagggcag ccatatcttc acgggcctct gaaaactcgc cttcctccat
     1321 cccctcaccc acgtaccagt gaacaaaggc acgcttggca tacatcaggt caaacttgtg
     1381 gtccaggcga gcccaggcct cagcaatggc tgtggtgttg ctcagcatgc acacagctct
     1441 ctgtaccttg gccaggtctc caccaggcac cacagtggga ggctggtagt tgatgccaac
     1501 cttgaagcca gtggggcacc aatccacaaa ctggatgctg cgcttggttt tgatggtggc
     1561 aatggcagca ttgacatctt tgggaaccac gtcaccacgg tacaacaggc agcaagccat
     1621 gtatttacca tggcgagggt cacatttcac catctggttg gctggctcaa agcaagcatt
     1681 ggtgatctct gctacagaaa gctgttcatg gtaggctttc tcagcagaga tgacaggggc
     1741 atatgtggcc agagggaagt ggatgcgggg gtagggcacc aggttggtct ggaattctgt
     1801 caggtcaaca ttcagggctc catcaaatct cagggaagca gtgatggagg acacaatctg
     1861 gctaataagg cggttaaggt tagtgtaggt tgggcgctcg atatcgaggt ttctacgaca
     1921 gatgtcatag atggcctcat tgtctaccat gaaggcacaa tcagagtgct ccagggtggt
     1981 gtgggtggtg aggatggagt tgtagggctc aactacagct gtggaaacct ggggtgctgg
     2041 gtaaatggag aactccagct tggacttctt gccataatca actgagagac gttccatgag
     2101 cagggaggtg aacccagaac cagttccccc accaaagctg tggaaaacca agaagccctg
     2161 aagaccggtg cactggtcag ccagcttgcg aattcggtcc aacacaaggt caatgatctc
     2221 cttgccaatg gtgtagtgcc ctcgggcata gttattggca gcatcttcct tgcctgtgat
     2281 gagctgctca gggtggaaga gctggcggta ggtgccagtg cgaacttcat caatgactgt
     2341 gggttccaag tctacaaaca cagcccgggg cacgtgcttg ccagcgcccg tctcactgaa
     2401 gaaggtgttg aaggagtcat ctcctccccc aatggtcttg tcacttggca tctggccatc
     2461 gggctggatg ccgtgttcca ggcagtagag ctcccagcag gcattgccaa tctggacacc
     2521 agcctggcca acgtggatgg agatgcactc acgcactcga gatctgagtc cggacttgta
     2581 cagctcgtcc atgccgccgg tggagtggcg gccctcggag cgttcgtact gttccaccac
     2641 ggtgtagtcc tcgttgtggg aggtgatgtc caacttgcgg tcgacgttgt aggcgccggg
     2701 catctgcacg ggcttcttgg ccttgtaggt ggtcttgaag tccgccaggt agcggccgcc
     2761 gtccttcagg cgcagggcca tcttaatgtc gcccttcagc acgccgtcct cggggtacaa
     2821 ccgctcggtg gacgcttccc agcccattgt cttcttctgc attacggggc cgtcaggagg
     2881 gaagttggtg ccgcggagct tcaccttgta gatcagggtg ccgtcctcca gggaggtgtc
     2941 ctgggtcacg gtcacggcgc cgccgtcctc gaagttcatc acgcgctccc acttgaagcc
     3001 ctcggggaag gactgcttat agtagtcggg gatgtcggcg gggtgcttgg tgaaggccct
     3061 ggagccgtac atgaactgag gggacaggat gtcccaggag aagggcaggg ggccaccctt
     3121 ggtcaccttc agcttggcgg tctgggtgcc ctcgtagggg cggccctcgc cctcgccctc
     3181 gatctcgaac tcgtggccgt tcatggagcc ctccatgtgc accttgaacc gcatgaactc
     3241 cttgatcact gcctcgccct tgctcaccat ggtggcgacc ggtagcgcta gcggatctga
     3301 cggttcacta aaccagctct gcttatatag acctcccacc gtacacgcct accgcccatt
     3361 tgcgtcaatg gggcggagtt gttacgacat tttggaaagt cccgttgatt ttggtgccaa
     3421 aacaaactcc cattgacgtc aatggggtgg agacttggaa atccccgtga gtcaaaccgc
     3481 tatccacgcc cattgatgta ctgccaaaac cgcatcacca tggtaatagc gatgactaat
     3541 acgtagatgt actgccaagt aggaaagtcc cataaggtca tgtactgggc ataatgccag
     3601 gcgggccatt taccgtcatt gacgtcaata gggggcgtac ttggcatatg atacacttga
     3661 tgtactgcca agtgggcagt ttaccgtaaa tactccaccc attgacgtca atggaaagtc
     3721 cctattggcg ttactatggg aacatacgtc attattgacg tcaatgggcg ggggtcgttg
     3781 ggcggtcagc caggcgggcc atttaccgta agttatgtaa cgcggaactc catatatggg
     3841 ctatgaacta atgaccccgt aattgattac tattaataac taatgcatgg cggtaatacg
     3901 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     3961 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     4021 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     4081 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     4141 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     4201 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     4261 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     4321 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     4381 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     4441 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     4501 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     4561 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     4621 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     4681 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     4741 acctgaggct atggcagggc ctgccgcccc gacgttggct gcgagccctg ggccttcacc
     4801 cgaacttggg gggtggggtg gggaaaagga agaaacgcgg gcgtattggc cccaatgggg
     4861 tctcggtggg gtatcgacag agtgccagcc ctgggaccga accccgcgtt tatgaacaaa
     4921 cgacccaaca ccgtgcgttt tattctgtct ttttattgcc gtcatagcgc gggttccttc
     4981 cggtattgtc tccttccgtg tttcagttag cctcccccta gggtgggcga agaactccag
     5041 catgagatcc ccgcgctgga ggatcatcca gccggcgtcc cggaaaacga ttccgaagcc
     5101 caacctttca tagaaggcgg cggtggaatc gaaatctcgt gatggcaggt tgggcgtcgc
     5161 ttggtcggtc atttcgaacc ccagagtccc gctcagaaga actcgtcaag aaggcgatag
     5221 aaggcgatgc gctgcgaatc gggagcggcg ataccgtaaa gcacgaggaa gcggtcagcc
     5281 cattcgccgc caagctcttc agcaatatca cgggtagcca acgctatgtc ctgatagcgg
     5341 tccgccacac ccagccggcc acagtcgatg aatccagaaa agcggccatt ttccaccatg
     5401 atattcggca agcaggcatc gccatgggtc acgacgagat cctcgccgtc gggcatgctc
     5461 gccttgagcc tggcgaacag ttcggctggc gcgagcccct gatgctcttc gtccagatca
     5521 tcctgatcga caagaccggc ttccatccga gtacgtgctc gctcgatgcg atgtttcgct
     5581 tggtggtcga atgggcaggt agccggatca agcgtatgca gccgccgcat tgcatcagcc
     5641 atgatggata ctttctcggc aggagcaagg tgagatgaca ggagatcctg ccccggcact
     5701 tcgcccaata gcagccagtc ccttcccgct tcagtgacaa cgtcgagcac agctgcgcaa
     5761 ggaacgcccg tcgtggccag ccacgatagc cgcgctgcct cgtcttgcag ttcattcagg
     5821 gcaccggaca ggtcggtctt gacaaaaaga accgggcgcc cctgcgctga cagccggaac
     5881 acggcggcat cagagcagcc gattgtctgt tgtgcccagt catagccgaa tagcctctcc
     5941 acccaagcgg ccggagaacc tgcgtgcaat ccatcttgtt caatcatgcg aaacgatcct
     6001 catcctgtct cttgatcgat cttt
//
