LOCUS       sequence-372973-       15420 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV helper plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15420)
  TITLE     AAV packaging plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15420)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAdDeltaF6
FEATURES             Location/Qualifiers
     source          1..15420
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(12663..12679)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12663..12679)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(12716..12736)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(12716..12734)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(12755..12771)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12755..12771)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12768..12790)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12779..12795
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12803..12809,12810..12827,12828..12833))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12848..12869
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12986..13003)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(13157..13745)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13237..13256)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13916..14707,14708..14776))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14539..14558
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(14777..14881)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     14949..14967
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        join(14994..14999,15000..15016,15017..15022)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
ORIGIN
        1 cacttgcaac atcacacttc cgccacacta ctacgtcacc cgccccgttc ccacgccccg
       61 cgccacgtca caaactccac cccctcatta tcatattggc ttcaatccaa aataatcatc
      121 aataatatac cttattttgg attgaagcca atatgataat gagggggtgg agtttgtgac
      181 gtggcgcggg gcgtgggaac ggggcgggtg acgtaggttt tagggcggag taacttgtat
      241 gtgttgggaa ttgtagtttt cttaaaatgg gaagtgacgt aacgtgggaa aacggaagtg
      301 acgatttgag gaagttgtgg gttttttggc tttcgtttct gggcgtaggt tcgcgtgcgg
      361 ttttctgggt gttttttgtg gactttaacc gttacgtcat tttttagtcc tatatatact
      421 cgctctgcac ttggcccttt tttacactgt gactgattga gctggtgccg tgtcgagtgg
      481 tgttttttta ataggttttc ttttttactg gtaaggctga ctgttatggc tgccgctgtg
      541 gaagcgctgt atgttgttct ggagcgggag ggtgctattt tgcctaggca ggagggtttt
      601 tcaggtgttt atgtgttttt ctctcctatt aattttgtta tacctcctat gggggctgta
      661 atgttgtctc tacgcctgcg ggtatgtatt cccccgggct atttcggtcg ctttttagca
      721 ctgaccgatg tgaatcaacc tgatgtgttt accgagtctt acattatgac tccggacatg
      781 accgaggagc tgtcggtggt gctttttaat cacggtgacc agttttttta cggtcacgcc
      841 ggcatggccg tagtccgtct tatgcttata agggttgttt ttcctgttgt aagacaggct
      901 tctaatgttt aaatgttttt ttgttatttt attttgtgtt tatgcagaaa cccgcagaca
      961 tgtttgagag aaaaatggtg tctttttctg tggtggttcc ggagcttacc tgcctttatc
     1021 tgcatgagca tgactacgat gtgctttctt ttttgcgcga ggctttgcct gattttttga
     1081 gcagcacctt gcattttata tcgccgccca tgcaacaagc ttacatcggg gctacgctgg
     1141 ttagcatagc tccgagtatg cgtgtcataa tcagtgtggg ttcttttgtc atggttcctg
     1201 gcggggaagt ggccgcgctg gtccgtgcag acctgcacga ttatgttcag ctggccctgc
     1261 gaagggacct acgggatcgc ggtatttttg ttaatgttcc gcttttgaat cttatacagg
     1321 tctgtgagga acctgaattt ttgcaatcat gattcgctgc ttgaggctga aggtggaggg
     1381 cgctctggag cagattttta caatggccgg acttaatatt cgggatttgc ttagagatat
     1441 attgagaagg tggcgagatg agaattattt gggcatggtt gaaggtgctg gaatgtttat
     1501 agaggagatt caccctgaag ggtttagcct ttacgtccac ttggacgtga gggccgtttg
     1561 ccttttggaa gccattgtgc aacatcttac aaatgccatt atctgttctt tggctgtaga
     1621 gtttgaccac gccaccggag gggagcgcgt tcacttaata gatcttcatt ttgaggtttt
     1681 ggataatctt ttggaataaa aaaaaaaaca tggttcttcc agctcttccc gctcctcccg
     1741 tgtgtgactc gcagaacgaa tgtgtaggtt ggctgggtgt ggcttattct gcggtggtgg
     1801 atgttatcag ggcagcggcg catgaaggag tttacataga acccgaagcc agggggcgcc
     1861 tggatgcttt gagagagtgg atatactaca actactacac agagcgatct aagcggcgag
     1921 accggagacg cagatctgtt tgtcacgccc gcacctggtt ttgcttcagg aaatatgact
     1981 acgtccggcg ttccatttgg catgacacta cgaccaacac gatctcggtt gtctcggcgc
     2041 actccgtaca gtagggatcg tctacctcct tttgagacag aaacccgcgc taccatactg
     2101 gaggatcatc cgctgctgcc cgaatgtaac actttgacaa tgcacaacgt gagttacgtg
     2161 cgaggtcttc cctgcagtgt gggatttacg ctgattcagg aatgggttgt tccctgggat
     2221 atggttctaa cgcgggagga gcttgtaatc ctgaggaagt gtatgcacgt gtgcctgtgt
     2281 tgtgccaaca ttgatatcat gacgagcatg atgatccatg gttacgagtc ctgggctctc
     2341 cactgtcatt gttccagtcc cggttccctg cagtgtatag ccggcgggca ggttttggcc
     2401 agctggttta ggatggtggt ggatggcgcc atgtttaatc agaggtttat atggtaccgg
     2461 gaggtggtga attacaacat gccaaaagag gtaatgttta tgtccagcgt gtttatgagg
     2521 ggtcgccact taatctacct gcgcttgtgg tatgatggcc acgtgggttc tgtggtcccc
     2581 gccatgagct ttggatacag cgccttgcac tgtgggattt tgaacaatat tgtggtgctg
     2641 tgctgcagtt actgtgctga tttaagtgag atcagggtgc gctgctgtgc ccggaggaca
     2701 aggcgcctta tgctgcgggc ggtgcgaatc atcgctgagg agaccactgc catgttgtat
     2761 tcctgcagga cggagcggcg gcggcagcag tttattcgcg cgctgctgca gcaccaccgc
     2821 cctatcctga tgcacgatta tgactctacc cccatgtagg cgtggacttc tccttcgccg
     2881 cccgttaagc aaccgcaagt tggacagcag cctgtggctc agcagctgga cagcgacatg
     2941 aacttaagtg agctgcccgg ggagtttatt aatatcactg atgagcgttt ggctcgacag
     3001 gaaaccgtgt ggaatataac acctaagaat atgtctgtta cccatgatat gatgcttttt
     3061 aaggccagcc ggggagaaag gactgtgtac tctgtgtgtt gggagggagg tggcaggttg
     3121 aatactaggg ttctgtgagt ttgattaagg tacggtgatc tgtataagct atgtggtggt
     3181 ggggctatac tactgaatga aaaatgactt gaaattttct gcaattgaaa aataaacacg
     3241 ttgaaacata acacaaacga ttctttattc ttgggcaatg tatgaaaaag tgtaagagga
     3301 tgtggcaaat atttcattaa tgtagttgtg gccagaccag tcccatgaaa atgacataga
     3361 gtatgcactt ggagttgtgt ctcctgtttc ctgtgtaccg tttagtgtaa tggttagtgt
     3421 tacaggttta gttttgtctc cgtttaagta aacttgactg acaatgttac ttttggcagt
     3481 tttaccgtga gattttggat aagctgatag gttaggcata aatccaacag cgtttgtata
     3541 ggctgtgcct tcagtaagat ctccatttct aaagttccaa tattctgggt ccaggaagga
     3601 attgtttagt agcactccat tttcgtcaaa tcttataata agatgagcac tttgaactgt
     3661 tccagatatt ggagccaaac tgcctttaac agccaaaact gaaactgtag caagtatttg
     3721 actgccacat tttgttaaga ccaaagtgag tttagcatct ttctctgcat ttagtctaca
     3781 gttaggagat ggagctggtg tggtccacaa agttagctta tcattatttt tgtttcctac
     3841 tgtaatggca cctgtgctgt caaaactaag gccagttcct agtttaggaa ccatagcctt
     3901 gtttgaatca aattctaggc catggccaat ttttgttttg aggggatttg tgtttggtgc
     3961 attaggtgaa ccaaattcaa gcccatctcc tgcattaatg gctatggctg tagcgtcaaa
     4021 catcaacccc ttggcagtgc ttaggttaac ctcaagcttt ttggaattgt ttgaagctgt
     4081 aaacaagtaa aggcctttgt tgtagttaat atccaagttg tgggctgagt ttataaaaag
     4141 agggccctgt cctagtctta gatttagttg gttttgagca tcaaacggat aactaacatc
     4201 aagtataagg cgtctgtttt gagaatcaat ccttagtcct cctgctacat taagttgcat
     4261 attgccttgt gaatcaaaac ccaaggctcc agtaacttta gtttgcaagg aagtattatt
     4321 aatagtcaca cctggaccag ttgctacggt caaagtgttt aggtcgtctg ttacatgcaa
     4381 aggagccccg tactttagtc ctagttttcc attttgtgta taaatgggct ctttcaagtc
     4441 aatgcccaag ctaccagtgg cagtagttag agggggtgag gcagtgatag taagggtact
     4501 gctatcggtg gtggtgaggg ggcctgatgt ttgcagggct agctttcctt ctgacactgt
     4561 gaggggtcct tgggtggcaa tgctaagttt ggagtcgtgc acggttagcg gggcctgtga
     4621 ttgcatggtg agtgtgttgc ccgcgaccat tagaggtgcg gcggcagcca cagttagggc
     4681 ttctgaggta actgtgaggg gtgcagatat ttccaggttt atgtttgact tggttttttt
     4741 gagaggtggg ctcacagtgg ttacattttg ggaggtaagg ttgccggcct cgtccagaga
     4801 gaggccgttg cccattttga gcgcaagcat gccattggag gtaactagag gttcggatag
     4861 gcgcaaagag agtaccccag ggggactctc ttgaaaccca ttgggggata caaagggagg
     4921 agtaagaaaa ggcacagttg gaggaccggt ttccgtgtca tatggataca cggggttgaa
     4981 ggtatcttca gacggtcttg cgcgcttcat ctgcaacaac atgaagatag tgggtgcgga
     5041 tggacaggaa caggaggaaa ctgacattcc atttagattg tggagaaagt ttgcagccag
     5101 gaggaagctg caataccaga gctgggagga gggcaaggag gtgctgctga ataaactgga
     5161 cagaaatttg ctaactgatt ttaagtaagt gatgctttat tatttttttt tattagttaa
     5221 agggaataag atccccgggt actctagtta attaactaga ggatcttgat gtaatccagg
     5281 gttaggacag ttgcaaatca cagtgagaac acagggtccc ctgtcccgct caactagcag
     5341 ggggcgctgg gtaaactccc gaatcaggct acgggcaagc tctccctggg cggtaagccg
     5401 gacgccgtgc gccgggccct cgatatgatc ctcgggcaat tcaaagtagc aaaactcacc
     5461 ggagtcgcgg gcaaagcact tgtggcggcg acagtggacc aggtgtttca ggcgcagttg
     5521 ctctgcctct ccacttaaca ttcagtcgta gccgtccgcc gagtccttta ccgcgtcaaa
     5581 gttaggaata aattgatccg gatagtggcc gggaggtccc gagaaggggt taaagtagac
     5641 cgatggcaca aactcctcaa taaattgcag agttccaatg cctccagagc gcggctcaga
     5701 ggacgaggtc tgcagagtta ggattgcctg acgaggcgtg aatgaagagc ggccggcgcc
     5761 gccgatctga aatgtcccgt ccggacggag accaagcgag gagctcaccg actcgtcgtt
     5821 gagctgaata cctcgccctc tgattgtcag gtgagttata ccctgcccgg gcgaccgcac
     5881 cctgtgacga aagccgcccg caagctgcgc ccctgagtta gtcatctgaa cttcggcctg
     5941 ggcgtctctg ggaagtacca cagtggtggg agcgggactt tcctggtaca ccagggcagc
     6001 gggccaacta cggggattaa ggttattacg aggtgtggtg gtaatagccg cctgttccag
     6061 gagaattcgg tttcggtggg cgcgtattcc gttgacccgg gatatcatgt ggggtcccgc
     6121 gctcatgtag tttattcggg ttgagtagtc ttgggcagct ccagccgcaa gtcccatttg
     6181 tggctggtaa ctccacatgt agggcgtggg aatttccttg ctcataatgg cgctgacaac
     6241 aggtgctggc gccgggtgtg gccgctggag atgacgtagt tttcgcgctt aaatttgaga
     6301 aagggcgcga aactagtcct taagagtcag cgcgcagtat ttactgaaga gagcctccgc
     6361 gtcttccagc gtgcgccgaa gctgatcttc gcttttgtga tacaggcagc tgcgggtgag
     6421 ggatcgcaga gacctgtttt ttattttcag ctcttgttct tggcccctgc tctgttgaaa
     6481 tatagcatac agagtgggaa aaatcctgtt tctaagctcg cgggtcgata cgggttcgtt
     6541 gggcgccaga cgcagcgctc ctcctcctgc tgctgccgcc gctgtggatt tcttgggctt
     6601 tgtcagagtc ttgctatccg gtcgcctttg cttctgtgtg gccgctgctg ttgctgccgc
     6661 tgccgccggt gcagtatggg ctgtagagat gacggtagta atgcaggatg ttacggggga
     6721 aggccacgcc gtgatggtag agaagaaagc ggcgggcgaa ggagatgttg cccccacagt
     6781 cttgcaagca agcaactatg gcgttcttgt gcccgcgcca tgagcggtag ccttggcgct
     6841 gttgttgctc ttgggctaac ggcggcggct gcttggactt accggccctg gttccagtgg
     6901 tgtcccatct acggttgggt cggcgaacgg gcagtgccgg cggcgcctga ggagcggagg
     6961 ttgtagccat gctggaaccg gttgccgatt tctggggcgc cggcgagggg aatgcgaccg
     7021 agggtgacgg tgtttcgtct gacacctctt cgacctcgga agcttcctcg tctaggctct
     7081 cccagtcttc catcatgtcc tcctcctcct cgtccaaaac ctcctctgcc tgactgtccc
     7141 agtattcctc ctcgtccgtg ggtggcggcg gcagctgcag cttctttttg ggtgccatcc
     7201 tgggaagcaa gggcccgcgg ctgctgctga tagggctgcg gcggcggggg gattgggttg
     7261 agctcctcgc cggactgggg gtccaagtaa accccccgtc cctttcgtag cagaaactct
     7321 tggcgggctt tgttgatggc ttgcaattgg ccaagaatgt ggccctgggt aatgacgcag
     7381 gcggtaagct ccgcattagg cgggcgggat tggtcttcgt agaacctaat ctcgtgggcg
     7441 tggtagtcct caggtacaaa tttgcgaagg taagccgacg tccacagccc cggagtgagt
     7501 ttcaaccccg gagccgcgga cttttcgtca ggcgagggac cctgcagctc aaaggtaccg
     7561 ataatttgac tttcgttaag cagctgcgaa ttgcaaacca gggagcggtg cggggtgcat
     7621 aggttgcagc gacagtgaca ctccagtaga ccgtcaccgc tcacgtcttc cattatgtca
     7681 gagtggtagg caaggtagtt ggctagctgc agaaggtagc agtggcccca aagcggcgga
     7741 gggcattcgc ggtacttaat gggcacaaag tcgctaggaa gtgcacagca ggtggcgggc
     7801 aagattcctg agcgctctag gataaagttc ctaaagttct gcaacatgct ttgactggtg
     7861 aagtctggca gaccctgttg cagggtttta agcaggcgtt cggggaaaat gatgtccgcc
     7921 aggtgcgcgg ccacggagcg ctcgttgaag gccgtccata ggtccttcaa gttttgcttt
     7981 agcagtttct gcagctcctt gaggttgcac tcctccaagc actgctgcca aacgcccatg
     8041 gccgtctgcc aggtgtagca tagaaataag taaacgcagt cgcggacgta gtcgcggcgc
     8101 gcctcgccct tgagcgtgga atgaagcacg ttttgcccaa ggcggttttc gtgcaaaatt
     8161 ccaaggtagg agaccaggtt gcagagctcc acgttggaga tcttgcaggc ctggcgtacg
     8221 tagccctgtc gaaaggtgta gtgcaatgtt tcctctagct tgcgctgcat ctccgggtca
     8281 gcaaagaacc gctgcatgca ctcaagctcc acggtaacga gcactgcggc catcattagt
     8341 ttgcgtcgct cctccaagtc ggcaggctcg cgcgtttgaa gccagcgcgc tagctgctcg
     8401 tcgccaactg cgggtaggcc ctcctctgtt tgttcttgca aatttgcatc cctctccagg
     8461 ggctgcgcac ggcgcacgat cagctcactc atgactgtgc tcatgacctt ggggggtagg
     8521 ttaagtgccg ggtaggcaaa gtgggtgacc tcgatgctgc gttttagtac ggctaggcgc
     8581 gcgttgtcac cctcgagttc caccaacact ccagagtgac tttcattttc gctgttttcc
     8641 tgttgcagag cgtttgccgc gcgcttctcg tcgcgtccaa gaccctcaaa gatttttggc
     8701 acttcgttga gcgaggcgat atcaggtatg acagcgccct gccgcaaggc cagctgcttg
     8761 tccgctcggc tgcggttggc acggcaggat aggggtatct tgcagttttg gaaaaagatg
     8821 tgataggtgg caagcacctc tggcacggca aatacggggt agaagttgag gcgcgggttg
     8881 ggctcgcatg tgccgttttc ttggcgtttg gggggtacgc gcggtgagaa taggtggcgt
     8941 tcgtaggcaa ggctgacatc cgctatggcg aggggcacat cgctgcgctc ttgcaacgcg
     9001 tcgcagataa tggcgcactg gcgctgcaga tgcttcaaca gcacgtcgtc tcccacatct
     9061 aggtagtcgc catgcctttc gtccccccgc ccgacttgtt cctcgtttgc ctctgcgttg
     9121 tcctggtctt gctttttatc ctctgttggt actgagcggt cctcgtcgtc ttcgcttaca
     9181 aaacctgggt cctgctcgat aatcacttcc tcctcctcaa gcgggggtgc ctcgacgggg
     9241 aaggtggtag gcgcgttggc ggcatcggtg gaggcggtgg tggcgaactc agagggggcg
     9301 gttaggctgt ccttcttctc gactgactcc atgatctttt tctgcctata ggagaaggaa
     9361 atggccagtc gggaagagga gcagcgcgaa accacccccg agcgcggacg cggtgcggcg
     9421 cgacgtcccc caaccatgga ggacgtgtcg tccccgtccc cgtcgccgcc gcctccccgg
     9481 gcgcccccaa aaaagcggat gaggcggcgt atcgagtccg aggacgagga agactcatca
     9541 caagacgcgc tggtgccgcg cacacccagc ccgcggccat cgacctcggc ggcggatttg
     9601 gccattgcgc ccaagaagaa aaagaagcgc ccttctccca agcccgagcg cccgccatca
     9661 ccagaggtaa tcgtggacag cgaggaagaa agagaagatg tggcgctaca aatggtgggt
     9721 ttcagcaacc caccggtgct aatcaagcat ggcaaaggag gtaagcgcac agtgcggcgg
     9781 ctgaatgaag acgacccagt ggcgcgtggt atgcggacgc aagaggaaga ggaagagccc
     9841 agcgaagcgg aaagtgaaat tacggtgatg aacccgctga gtgtgccgat cgtgtctgcg
     9901 tgggagaagg gcatggaggc tgcgcgcgcg ctgatggaca agtaccacgt ggataacgat
     9961 ctaaaggcga acttcaaact actgcctgac caagtggaag ctctggcggc cgtatgcaag
    10021 acctggctga acgaggagca ccgcgggttg cagctgacct tcaccagcaa caagaccttt
    10081 gtgacgatga tggggcgatt cctgcaggcg tacctgcagt cgtttgcaga ggtgacctac
    10141 aagcatcacg agcccacggg ctgcgcgttg tggctgcacc gctgcgctga gatcgaaggc
    10201 gagcttaagt gtctacacgg aagcattatg ataaataagg agcacgtgat tgaaatggat
    10261 gtgacgagcg aaaacgggca gcgcgcgctg aaggagcagt ctagcaaggc caagatcgtg
    10321 aagaaccggt ggggccgaaa tgtggtgcag atctccaaca ccgacgcaag gtgctgcgtg
    10381 cacgacgcgg cctgtccggc caatcagttt tccggcaagt cttgcggcat gttcttctct
    10441 gaaggcgcaa aggctcaggt ggcttttaag cagatcaagg cttttatgca ggcgctgtat
    10501 cctaacgccc agaccgggca cggtcacctt ttgatgccac tacggtgcga gtgcaactca
    10561 aagcctgggc acgcgccctt tttgggaagg cagctaccaa agttgactcc gttcgccctg
    10621 agcaacgcgg aggacctgga cgcggatctg atctccgaca agagcgtgct ggccagcgtg
    10681 caccacccgg cgctgatagt gttccagtgc tgcaaccctg tgtatcgcaa ctcgcgcgcg
    10741 cagggcggag gccccaactg cgacttcaag atatcggcgc ccgacctgct aaacgcgttg
    10801 gtgatggtgc gcagcctgtg gagtgaaaac ttcaccgagc tgccgcggat ggttgtgcct
    10861 gagtttaagt ggagcactaa acaccagtat cgcaacgtgt ccctgccagt ggcgcatagc
    10921 gatgcgcggc agaacccctt tgatttttaa acggcgcaga cggcaagggt gggggtaaat
    10981 aatcacccga gagtgtacaa ataaaagcat ttgcctttat tgaaagtgtc tctagtacat
    11041 tatttttaca tgtttttcaa gtgacaaaaa gaagtggcgc tcctaatctg cgcactgtgg
    11101 ctgcggaagt agggcgagtg gcgctccagg aagctgtaga gctgttcctg gttgcgacgc
    11161 agggtgggct gtacctgggg actgttgagc atggagttgg gtaccccggt aataaggttc
    11221 atggtggggt tgtgatccat gggagtttgg ggccagttgg caaaggcgtg gagaaacatg
    11281 cagcagaata gtccacaggc ggccgagttg ggcccctgta cgctttgggt ggacttttcc
    11341 agcgttatac agcggtcggg ggaagaagca atggcgctac ggcgcaggag tgactcgtac
    11401 tcaaactggt aaacctgctt gagtcgctgg tcagaaaagc caaagggctc aaagaggtag
    11461 catgtttttg agtgcgggtt ccaggcaaag gccatccagt gtacgccccc agtctcggtc
    11521 cgagactcga accgggggtc ccgcgactca acccttggaa aataaccctc cggctacagg
    11581 gagcgagcca cttaatgctt tcgctttcca gcctaaccgc ttacgctgcg cgcggccagt
    11641 ggccaaaaaa gctagcgcag cagccgccgc gcctggaagg aagccaaaag gagcactccc
    11701 ccgttgtctg acgtcgcaca cctgggttcg acacgcgggc ggtaaccgca tggatcacgg
    11761 cggacggccg gatacggggc tcgaaccccg gtcgtccgcc atgataccct tgcgaattta
    11821 tccaccagac cacggaagag tgcccgctta caggctctcc ttttgcacgc tagagcgtca
    11881 acgattgcgc gcgcctgacc ggccagagcg tcccgaccat ggagcacttt ttgccgctgc
    11941 gcaacatctg gaaccgcgtc cgcgactttc cgcgcgcctc caccaccgcc gccggcatca
    12001 cctggatgtc caggtacatc tacggatatc atcgccttat gttggaagat ctcgcccccg
    12061 gagccccggc caccctacgc tggcccctct accgccagcc gccgccgcac tttttggtgg
    12121 gataccagta cctggtgcgg acttgcaacg actacgtatt tgactcgagg gcttactcgc
    12181 gtctcaggta caccgagctc tcgcagccgg gtcaccagac cgttaactgg tccgttatgg
    12241 ccaactgcac ttacaccatc aacacgggcg cataccaccg ctttgtggac atggatgact
    12301 tccagtctac cctcacgcag gtgcagcagg ccatattagc cgagcgcgtt gtcgccgacc
    12361 tagccctgct tcagccgatg aggggcttcg gggtcacacg catgggagga agagggcgcc
    12421 acctacggcc aaactccgcc gccgccgcag cgatagatgc aagagatgca ggacaagagg
    12481 aaggagaaga agaagtgccg gtagaaaggc tcatgcaaga ctactacaaa gacctgcgcc
    12541 gatgtcaaaa cgaagcctgg ggcatggccg accgcctgcg cattcagcag gccggaccca
    12601 aggacatggt gcttctgtcg acggtatcga taagcttgat atcgaattcc tgcagcccgg
    12661 gggatccact agttctagag cggccgccac cgcggtggag ctccagcttt tgttcccttt
    12721 agtgagggtt aattgcgcgc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
    12781 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
    12841 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
    12901 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
    12961 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
    13021 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
    13081 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
    13141 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
    13201 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
    13261 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
    13321 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
    13381 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
    13441 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
    13501 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
    13561 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
    13621 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
    13681 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
    13741 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
    13801 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
    13861 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
    13921 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
    13981 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
    14041 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
    14101 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
    14161 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
    14221 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
    14281 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
    14341 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
    14401 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
    14461 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
    14521 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
    14581 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
    14641 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
    14701 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
    14761 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
    14821 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
    14881 gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa
    14941 cctataaaaa taggcgtatc acgaggccct ttcgtcttca agaattctca tgtttgacag
    15001 cttatcatcg ataagcttta atgcggtagt ttatcacagt taaattgcta acgcagtcag
    15061 gcaccgtgta tgaaatctaa caatgcgctc atcgtcatcc tcggcaccgt caccctggat
    15121 gctgtaggca taggcttggt tatgccggta ctgccgggcc tcttgcggga tatcgtccat
    15181 tccgacagca tcgccagtca ctatggcgtg ctgctagcgc tatatgcgtt gatgcaattt
    15241 ctatgcgcac ccgttctcgg agcactgtcc gaccgctttg gccgccgccc agtcctgctc
    15301 gcttcgctac ttggagccac tatcgactac gcgatcatgg cgaccacacc cgtcctgtgg
    15361 atccggcgca caccaaaaac gtcacttttg ccacatccgt cgcttacatg tgttccgcca
//
