LOCUS       sequence-372974-       14335 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  RUBY under the control of CaMV 35S promoter/marker for 
            transformation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14335)
  AUTHORS   He Y, Zhang T, Sun H, Zhan H, Zhao Y
  TITLE     A reporter for noninvasively monitoring gene expression and plant 
            transformation.
  JOURNAL   Hortic Res. 2020 Sep 19;7:152. doi: 10.1038/s41438-020-00390-1. 
            eCollection 2020.
  PUBMED    33024566
REFERENCE   2  (bases 1 to 14335)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Hortic 
            Res."; date: "2020-09-19"; volume: "7:152. doi"; pages: " 
            10.1038/s41438-020-00390-1. eCollection 2020"
COMMENT     Sequence Label: 35S:RUBY
FEATURES             Location/Qualifiers
     source          1..14335
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             203..994
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1240..1828
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1729..1748
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1982..1999
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    2014..2154
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2080..2099)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2240..2262
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      2498..2692
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(2758..3831)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(4260..4889)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    6220..6244
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        6259..6935
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     6872..6892
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             6944..10894
                     /label=RUBY
     CDS             8444..8500
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             9335..9391
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     promoter        11598..11777
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        11630..11813
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     terminator      12928..13180
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(13755..13772)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(13755..13771)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13764..13786)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    13993..14017
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gtccagaacc ttgaccgaac gcagcggtgg taacggcgca gtggcggttt tcatggcttg
       61 ttatgactgt ttttttgggg tacagtctat gcctcgggca tccaagcagc aagcgcgtta
      121 cgccgtgggt cgatgtttga tgttatggag cagcaacgat gttacgcagc agggcagtcg
      181 ccctaaaaca aagttaaaca tcatggggga agcggtgatc gccgaagtat cgactcaact
      241 atcagaggta gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt
      301 gtacggctcc gcagtggatg gcggcctgaa gccacacagt gatattgatt tgctggttac
      361 ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg atcaacgacc ttttggaaac
      421 ttcggcttcc cctggagaga gcgagattct ccgcgctgta gaagtcacca ttgttgtgca
      481 cgacgacatc attccgtggc gttatccagc taagcgcgaa ctgcaatttg gagaatggca
      541 gcgcaatgac attcttgcag gtatcttcga gccagccacg atcgacattg atctggctat
      601 cttgctgaca aaagcaagag aacatagcgt tgccttggta ggtccagcgg cggaggaact
      661 ctttgatccg gttcctgaac aggatctatt tgaggcgcta aatgaaacct taacgctatg
      721 gaactcgccg cccgactggg ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat
      781 ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat
      841 ggagcgcctg ccggcccagt atcagcccgt catacttgaa gctagacagg cttatcttgg
      901 acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg gaagaatttg tccactacgt
      961 gaaaggcgag atcaccaagg tagtcggcaa ataatgtcta gctagaaatt cgttcaagcc
     1021 gacgccgctt cgcggcgcgg cttaactcaa gcgttagatg cactaagcac ataattgctc
     1081 acagccaaac tatcaggtca agtctgcttt tattattttt aagcgtgcat aataagccct
     1141 acacaaattg ggagatatat catgcatgac caaaatccct taacgtgagt tttcgttcca
     1201 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     1261 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     1321 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     1381 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     1441 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     1501 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     1561 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     1621 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     1681 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     1741 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     1801 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     1861 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     1921 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     1981 cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca
     2041 tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc
     2101 atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga
     2161 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
     2221 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
     2281 aaacgcgcga ggcagggtgc cttgatgtgg gcgccggcgg tcgagtggcg acggcgcggc
     2341 ttgtccgcgc cctggtagat tgcctggccg taggccagcc atttttgagc ggccagcggc
     2401 cgcgataggc cgacgcgaag cggcggggcg tagggagcgc agcgaccgaa gggtaggcgc
     2461 tttttgcagc tcttcggctg tgcgctggcc agacagttat gcacaggcca ggcgggtttt
     2521 aagagtttta ataagtttta aagagtttta ggcggaaaaa tcgccttttt tctcttttat
     2581 atcagtcact tacatgtgtg accggttccc aatgtacggc tttgggttcc caatgtacgg
     2641 gttccggttc ccaatgtacg gctttgggtt cccaatgtac gtgctatcca caggaaagag
     2701 accttttcga cctttttccc ctgctagggc aatttgccct agcatctgct ccgtacatta
     2761 ggaaccggcg gatgcttcgc cctcgatcag gttgcggtag cgcatgacta ggatcgggcc
     2821 agcctgcccc gcctcctcct tcaaatcgta ctccggcagg tcatttgacc cgatcagctt
     2881 gcgcacggtg aaacagaact tcttgaactc tccggcgctg ccactgcgtt cgtagatcgt
     2941 cttgaacaac catctggctt ctgccttgcc tgcggcgcgg cgtgccaggc ggtagagaaa
     3001 acggccgatg ccgggatcga tcaaaaagta atcggggtga accgtcagca cgtccgggtt
     3061 cttgccttct gtgatctcgc ggtacatcca atcagctagc tcgatctcga tgtactccgg
     3121 ccgcccggtt tcgctcttta cgatcttgta gcggctaatc aaggcttcac cctcggatac
     3181 cgtcaccagg cggccgttct tggccttctt cgtacgctgc atggcaacgt gcgtggtgtt
     3241 taaccgaatg caggtttcta ccaggtcgtc tttctgcttt ccgccatcgg ctcgccggca
     3301 gaacttgagt acgtccgcaa cgtgtggacg gaacacgcgg ccgggcttgt ctcccttccc
     3361 ttcccggtat cggttcatgg attcggttag atgggaaacc gccatcagta ccaggtcgta
     3421 atcccacaca ctggccatgc cggccggccc tgcggaaacc tctacgtgcc cgtctggaag
     3481 ctcgtagcgg atcacctcgc cagctcgtcg gtcacgcttc gacagacgga aaacggccac
     3541 gtccatgatg ctgcgactat cgcgggtgcc cacgtcatag agcatcggaa cgaaaaaatc
     3601 tggttgctcg tcgcccttgg gcggcttcct aatcgacggc gcaccggctg ccggcggttg
     3661 ccgggattct ttgcggattc gatcagcggc cgcttgccac gattcaccgg ggcgtgcttc
     3721 tgcctcgatg cgttgccgct gggcggcctg cgcggccttc aacttctcca ccaggtcatc
     3781 acccagcgcc gcgccgattt gtaccgggcc ggatggtttg cgaccgctca cgccgattcc
     3841 tcgggcttgg gggttccagt gccattgcag ggccggcaga caacccagcc gcttacgcct
     3901 ggccaaccgc ccgttcctcc acacatgggg cattccacgg cgtcggtgcc tggttgttct
     3961 tgattttcca tgccgcctcc tttagccgct aaaattcatc tactcattta ttcatttgct
     4021 catttactct ggtagctgcg cgatgtattc agatagcagc tcggtaatgg tcttgccttg
     4081 gcgtaccgcg tacatcttca gcttggtgtg atcctccgcc ggcaactgaa agttgacccg
     4141 cttcatggct ggcgtgtctg ccaggctggc caacgttgca gccttgctgc tgcgtgcgct
     4201 cggacggccg gcacttagcg tgtttgtgct tttgctcatt ttctctttac ctcattaact
     4261 caaatgagtt ttgatttaat ttcagcggcc agcgcctgga cctcgcgggc agcgtcgccc
     4321 tcgggttctg attcaagaac ggttgtgccg gcggcggcag tgcctgggta gctcacgcgc
     4381 tgcgtgatac gggactcaag aatgggcagc tcgtacccgg ccagcgcctc ggcaacctca
     4441 ccgccgatgc gcgtgccttt gatcgcccgc gacacgacaa aggccgcttg tagccttcca
     4501 tccgtgacct caatgcgctg cttaaccagc tccaccaggt cggcggtggc ccatatgtcg
     4561 taagggcttg gctgcaccgg aatcagcacg aagtcggctg ccttgatcgc ggacacagcc
     4621 aagtccgccg cctggggcgc tccgtcgatc actacgaagt cgcgccggcc gatggccttc
     4681 acgtcgcggt caatcgtcgg gcggtcgatg ccgacaacgg ttagcggttg atcttcccgc
     4741 acggccgccc aatcgcgggc actgccctgg ggatcggaat cgactaacag aacatcggcc
     4801 ccggcgagtt gcagggcgcg ggctagatgg gttgcgatgg tcgtcttgcc tgacccgcct
     4861 ttctggttaa gtacagcgat aaccttcatg cgttcccctt gcgtatttgt ttatttactc
     4921 atcgcatcat atacgcagcg accgcatgac gcaagctgtt ttactcaaat acacatcacc
     4981 tttttagacg gcggcgctcg gtttcttcag cggccaagct ggccggccag gccgccagct
     5041 tggcatcaga caaaccggcc aggatttcat gcagccgcac ggttgagacg tgcgcgggcg
     5101 gctcgaacac gtacccggcc gcgatcatct ccgcctcgat ctcttcggta atgaaaaacg
     5161 gttcgtcctg gccgtcctgg tgcggtttca tgcttgttcc tcttggcgtt cattctcggc
     5221 ggccgccagg gcgtcggcct cggtcaatgc gtcctcacgg aaggcaccgc gccgcctggc
     5281 ctcggtgggc gtcacttcct cgctgcgctc aagtgcgcgg tacagggtcg agcgatgcac
     5341 gccaagcagt gcagccgcct ctttcacggt gcggccttcc tggtcgatca gctcgcgggc
     5401 gtgcgcgatc tgtgccgggg tgagggtagg gcgggggcca aacttcacgc ctcgggcctt
     5461 ggcggcctcg cgcccgctcc gggtgcggtc gatgattagg gaacgctcga actcggcaat
     5521 gccggcgaac acggtcaaca ccatgcggcc ggccggcgtg gtggtgtcgg cccacggctc
     5581 tgccaggcta cgcaggcccg cgccggcctc ctggatgcgc tcggcaatgt ccagtaggtc
     5641 gcgggtgctg cgggccaggc ggtctagcct ggtcactgtc acaacgtcgc cagggcgtag
     5701 gtggtcaagc atcctggcca gctccgggcg gtcgcgcctg gtgccggtga tcttctcgga
     5761 aaacagcttg gtgcagccgg ccgcgtgcag ttcggcccgt tggttggtca agtcctggtc
     5821 gtcggtgctg acgcgggcat agcccagcag gccagcggcg gcgctcttgt tcatggcgta
     5881 atgtctccgg ttctagtcgc aagtattcta ctttatgcga ctaaaacacg cgacaagaaa
     5941 acgccaggaa aagggcaggg cggcagcctg tcgcgtaact taggacttgt gcgacatgtc
     6001 gttttcagaa gacggctgca ctgaacgtca gaagccgact gcactatagc agcggagggg
     6061 ttggatcaaa gtactttaaa gtactttaaa gtactttaaa gtactttgat cccgagggga
     6121 accctgtggt tggcatgcac atacaaatgg acgaacggat aaaccttttc acgccctttt
     6181 aaatatccga ttattctaat aaacgctctt ttctcttagg tttacccgcc aatatatcct
     6241 gtcaaacact gatagttttg agacttttca acaaagggta atatcgggaa acctcctcgg
     6301 attccattgc ccagctatct gtcacttcat caaaaggaca gtagaaaagg aaggtggcac
     6361 ctacaaatgc catcattgcg ataaaggaaa ggctatcgtt caagatgcct ctgccgacag
     6421 tggtcccaaa gatggacccc cacccacgag gagcatcgtg gaaaaagaag acgttccaac
     6481 cacgtcttca aagcaagtgg attgatgtga acatggtgga gcacgacact ctcgtctact
     6541 ccaagaatat caaagataca gtctcagaag accaaagggc tattgagact tttcaacaaa
     6601 gggtaatatc gggaaacctc ctcggattcc attgcccagc tatctgtcac ttcatcaaaa
     6661 ggacagtaga aaaggaaggt ggcacctaca aatgccatca ttgcgataaa ggaaaggcta
     6721 tcgttcaaga tgcctctgcc gacagtggtc ccaaagatgg acccccaccc acgaggagca
     6781 tcgtggaaaa agaagacgtt ccaaccacgt cttcaaagca agtggattga tgtgatatct
     6841 ccactgacgt aagggatgac gcacaatccc actatccttc gcaagaccct tcctctatat
     6901 aaggaagttc atttcatttg gagaggacac gctgaaaact agtatggatc atgcgaccct
     6961 cgccatgatc ctcgcgatct ggttcatcag cttccacttc atcaagctgc tgttctccca
     7021 gcagaccacc aagctgcttc cgccaggacc aaagccgctt ccgatcatcg gcaacatcct
     7081 tgaggtgggc aagaagccgc atcggtcctt cgccaacctc gccaagattc acggcccact
     7141 catttccctc agactcggct ctgtgaccac catcgttgtg tcctctgccg acgtggccaa
     7201 agagatgttc ctcaagaagg atcacccgct ctccaaccgc acgatcccga atagtgttac
     7261 agccggcgac caccacaagc tcaccatgtc ttggctcccg gtgtctccga agtggcgcaa
     7321 cttccgcaag attaccgccg tgcatctgct ctccccacag agactcgatg cctgccagac
     7381 attcaggcac gccaaggtgc agcagctcta cgagtacgtt caagagtgcg cccagaaagg
     7441 ccaggccgtg gatattggca aggccgcctt tacgaccagc ctcaacctcc tcagcaagct
     7501 gttcttcagc gtcgagctgg cgcaccacaa gtcccatacc agccaagagt tcaaagagct
     7561 gatctggaac atcatggaag atataggcaa gccgaactac gccgactact tcccgattct
     7621 cggctgcgtt gacccatctg gcattagaag aaggctcgcc tgctccttcg acaagctgat
     7681 cgccgtgttc cagggcatca tctgcgagag actcgcccca gattcctcca ccacaactac
     7741 caccaccacc gacgacgtgc tcgatgtgct cctccagctg ttcaagcaga acgagctgac
     7801 gatgggcgag atcaaccacc tcctcgtgga catcttcgac gccggcaccg ataccacatc
     7861 ctccacattc gagtgggtga tgaccgagct gatccgcaat ccagagatga tggaaaaggc
     7921 ccaagaggaa atcaagcagg tcctcggcaa ggacaagcag atccaagagt ccgacatcat
     7981 caacctgccg tacctccagg cgatcatcaa agagacactc cgcctccatc cgccgaccgt
     8041 gttcttgctc ccaagaaagg ccgacaccga tgtcgagctg tacggctaca tcgtgccgaa
     8101 ggatgcccag atcctcgtga acctctgggc cattggcagg gacccaaacg cctggcagaa
     8161 cgccgatatt ttcagcccag agcgcttcat cggctgcgag atcgatgtta agggccgcga
     8221 tttcggcctc cttccatttg gcgctggccg cagaatttgc ccaggcatga atctcgccat
     8281 caggatgctc accctcatgc tcgccacact cctccagttc ttcaactgga agctcgaagg
     8341 cgacatctcc ccgaaggacc tcgacatgga cgagaagttc ggcattgcgc tccaaaagac
     8401 caagccgctc aagctcatcc cgattccgcg ctacggtagc ggagctacca attttagcct
     8461 ccttaagcag gcaggtgatg tagaagagaa ccccgggcct atgaagatga tgaacggcga
     8521 ggacgccaac gaccagatga tcaaagagtc cttcttcatc acccacggca acccgatcct
     8581 caccgtcgag gatacacatc cgctcaggcc gttcttcgag acatggcgcg agaagatttt
     8641 ctccaagaag ccgaaggcca tcctcatcat ctccggccac tgggagacag tgaagccaac
     8701 cgtgaacgcc gtgcacatca acgacaccat ccacgacttc gacgactacc cagccgccat
     8761 gtaccagttc aagtacccag ctccaggcga gccagagctt gcgagaaagg tggaagagat
     8821 cctcaagaag tccgggttcg agacagccga gacagaccaa aagaggggcc ttgatcacgg
     8881 cgcctgggtt ccactcatgc tcatgtatcc agaggcggac atcccggtgt gccagctctc
     8941 agttcagcca catctcgacg gcacctacca ctacaatctc ggcagagccc tcgcgccgct
     9001 caagaatgat ggcgtgctca ttattggctc cggcagcgcc acacatccac tcgatgagac
     9061 accgcactac ttcgatggtg ttgccccttg ggccgctgcc ttcgattctt ggcttaggaa
     9121 ggccctcatc aacggccgct tcgaggaagt gaacatctac gagagcaagg ccccgaactg
     9181 gaagctcgcc catccatttc cagagcactt ctacccgctc cacgttgtgc tcggcgctgc
     9241 tggtgaaaag tggaaggccg agctgatcca ctcctcctgg gatcatggca cactttgcca
     9301 cggctcctac aagttcacct ccgccggatc cggagcaacc aactttagcc tgctcaagca
     9361 agcaggagat gttgaggaaa atcctggccc catgaccgcc atcaagatga acaccaacgg
     9421 cgagggcgag acacagcaca tcctcatgat cccgttcatg gcgcagggcc acctcaggcc
     9481 atttctcgaa ctcgccatgt tcctctacaa gcgctcccac gtgatcatca ccctgctcac
     9541 aactccgctc aatgccggct tcctcaggca cctccttcac caccattcct actcctccag
     9601 cggcatcagg atcgtcgagc tgccattcaa ctccaccaac cacggactcc caccgggcat
     9661 cgagaacacc gataagctca cactcccgct cgtggtgtcc ctcttccatt ccaccatcag
     9721 cctcgatccg cacctccgcg attacatctc caggcatttc agcccagcca ggccaccact
     9781 ctgcgtgatc catgatgtgt tcctcggctg ggttgaccag gtggccaagg atgtgggctc
     9841 tacaggcgtg gtgttcacaa caggcggcgc ttatggcaca tccgcctacg tgtccatctg
     9901 gaacgatctc ccgcaccaga actactccga cgaccaagag ttcccgctgc caggcttccc
     9961 agagaaccat aagttccgca ggtcccagct ccatcggttc ctcagatatg ccgacggctc
    10021 cgacgattgg tccaagtatt tccagccgca gctccgccag tccatgaagt cttttggctg
    10081 gctctgcaac tccgtggaag agatcgagac actcggcttc tccatcctcc gcaactacac
    10141 caagctgccg atctggggca tcggcccact tattgcttcc ccagtgcagc actcctcctc
    10201 cgacaacaat tcaacaggcg ccgagttcgt gcagtggctc agcctcaaag agccggactc
    10261 cgtcctctac atctccttcg gctcccagaa cacgatcagc ccgacgcaga tgatggaact
    10321 cgctgctggc cttgagtcct ccgagaagcc attcctctgg gtgatcagag ccccgttcgg
    10381 cttcgacatc aacgaagaga tgcgcccaga gtggctgcca gagggctttg aggaacgcat
    10441 gaaggtgaag aaacagggca agctcgtgta caagctcggc ccgcagcttg agatcctcaa
    10501 ccatgaatcc atcggcggct ttctcaccca ctgcggatgg aacagcatcc ttgagtctct
    10561 tcgcgagggc gttccgatgc ttggatggcc acttgctgcc gagcaggcct acaacctcaa
    10621 gtacctcgaa gatgagatgg gcgtcgcggt tgagcttgct agaggcctcg aaggcgagat
    10681 ctccaaagag aaggtcaagc gcatcgtcga gatgatcctt gagcgcaacg agggctccaa
    10741 aggctgggag atgaagaatc gcgccgtgga aatgggcaaa aagctcaagg acgccgtgaa
    10801 cgaggaaaaa gagctgaagg gctcctccgt gaaggcgatc gacgatttcc tcgacgccgt
    10861 catgcaggcc aaacttgagc caagcctcca gtgatagtga atatgaagat gaagatgaaa
    10921 tatttggtgt gtcaaataaa aaggttgtgt gcttaagttt gtgttttttt cttggcttgt
    10981 tgtgttatga atttgtggct ttttctaata ttaaatgaat gtaacatctc attataatga
    11041 ataaacaaat gtttctataa tccattgtga atgttttgtt ggatctcttc tccagcatat
    11101 aactactgta tgtgctatgg tatggactat ggaatatgat taaagataag acctaactga
    11161 gtaagctagc ttgttcgagt attatggcat tgggaaaact gtttttcttg taccatttgt
    11221 tgtgcttgta atttactgtg ttttttattc ggttttcgct atcgaactgt gaaatggaaa
    11281 tggatggaga agagttaatg aatgatatgg tccttttgtt cattctcaaa ttaatattat
    11341 ttgttttttc tcttatttgt tgtgtgttga atttgaaatt ataagagata tgcaaacatt
    11401 ttgttttgag taaaaatgtg tcaaatcgtg gcctctaatg accgaagtta atatgaggag
    11461 taaaacacta gatccccaaa caagcttgaa actgaaggcg ggaaacgaca atctgatcat
    11521 gagcggagaa ttaagggagt cacgttatga cccccgccga tgacgcggga caagccgttt
    11581 tacgtttgga actgacagaa ccgcaacgtt gaaggagcca ctcagccgcg ggtttctgga
    11641 gtttaatgag ctaagcacat acgtcagaaa ccattattgc gcgttcaaaa gtcgcctaag
    11701 gtcactatca gctagcaaat atttcttgtc aaaaatgctc cactgacgtt ccataaattc
    11761 ccctcggtat ccaattagag tctcatattc actctcaatc caaataatct gcaccggatc
    11821 cgctagagga tctcgacctg caagatcccg gggggcaatg agatatgaaa aagcctgaac
    11881 tcaccgcgac gtctgtcgag aagtttctga tcgaaaagtt cgacagcgtc tccgacctga
    11941 tgcagctctc ggagggcgaa gaatctcgtg ctttcagctt cgatatagga gggcgtggat
    12001 atgtcctgcg ggtaaatagc tgcgccgatg gtttctacaa agatcgttat gtttatcggc
    12061 actttgcatc ggccgcgctc ccgattccgg aagtgcttga cattggggaa ttcagcgaga
    12121 gcctgaccta ttgcatctcc cgccgtgcac agggtgtcac gttgcaagac ctgcctgaaa
    12181 ccgaactgcc cgctgttctg cagccggtcg cggaggccat ggatgcgatc gctgcggccg
    12241 atcttagcca gacgagcggg ttcggcccat tcggaccgca aggaatcggt caatacacta
    12301 catggcgtga tttcatatgc gcgattgctg atccccatgt gtatcactgg caaactgtga
    12361 tggacgacac cgtcagtgcg tccgtcgcgc aggctctcga tgagctgatg ctttgggccg
    12421 aggactgccc cgaagtccgg cacctcgtgc acgcggattt cggctccaac aatgtcctga
    12481 cggacaatgg ccgcataaca gcggtcattg actggagcga ggcgatgttc ggggattccc
    12541 aatacgaggt cgccaacatc ttcttctgga ggccgtggtt ggcttgtatg gagcagcaga
    12601 cgcgctactt cgagcggagg catccggagc ttgcaggatc gccgcggctc cgggcgtata
    12661 tgctccgcat tggtcttgac caactctatc agagcttggt tgacggcaat ttcgatgatg
    12721 cagcttgggc gcagggtcga tgcgacgcaa tcgtccgatc cggagccggg actgtcgggc
    12781 gtacacaaat cgcccgcaga agcgcggccg tctggaccga tggctgtgta gaagtactcg
    12841 ccgatagtgg aaaccgacgc cccagcactc gtccgggatc ttggaggtga tgtaacatga
    12901 tcacaagctg atcccccgaa tttccccgat cgttcaaaca tttggcaata aagtttctta
    12961 agattgaatc ctgttgccgg tcttgcgatg attatcatat aatttctgtt gaattacgtt
    13021 aagcatgtaa taattaacat gtaatgcatg acgttattta tgagatgggt ttttatgatt
    13081 agagtcccgc aattatacat ttaatacgcg atagaaaaca aaatatagcg cgcaaactag
    13141 gataaattat cgcgcgcggt gtcatctatg ttactagatc gggaattgat cccccctcga
    13201 cagcttgcat gccagcttgg gctgcaggtc gacgcgtcaa ttgatatcga tccaggcctc
    13261 ccagctttcg tccgtatcat cggtttcgac aacgttcgtc aagttcaatg catcagtttc
    13321 attgcccaca caccagaatc ctactaagtt tgagtattat ggcattggaa aagctgtttt
    13381 cttctatcat ttgttctgct tgtaatttac tgtgttcttt cagtttttgt tttcggacat
    13441 caaaatgcaa atggatggat aagagttaat aaatgatatg gtccttttgt tcattctcaa
    13501 attattatta tctgttgttt ttactttaat gggttgaatt taagtaagaa aggaactaac
    13561 agtgtgatat taaggtgcaa tgttagacat ataaaacagt ctttcacctc tctttggtta
    13621 tgtcttgaat tggtttgttt cttcacttat ctgtgtaatc aagtttacta tgagtctatg
    13681 atcaagtaat tatgcaatca agttaagtac agtataggct ttttgtgtcg agggggtacc
    13741 gagtcgagga attcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
    13801 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
    13861 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgggta ccgagctcga
    13921 attcaattcg gcgttaattc agtacattaa aaacgtccgc aatgtgttat taagttgtct
    13981 aagcgtcaat ttgtttacac cacaatatat cctgccacca gccagccaac agctccccga
    14041 ccggcagctc ggcacaaaat caccactcga tacaggcagc ccatcagtcc gggacggcgt
    14101 cagcgggaga gccgttgtaa ggcggcagac tttgctcatg ttaccgatgc tattcggaag
    14161 aacggcaact aagctgccgg gtttgaaaca cggatgatct cgcggagggt agcatgttga
    14221 ttgtaacgat gacagagcgt tgctgcctgt gatcaattcg ggcacgaacc cagtggacat
    14281 aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat gcgctcacgc aactg
//
