LOCUS       sequence-372976-       10397 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of shRNA targeting Anillin + reconstitution of
            Anillin delta Actin binding domain .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10397)
  AUTHORS   Budnar S, Husain KB, Gomez GA, Naghibosadat M, Varma A, Verma S, 
            Hamilton NA, Morris RG, Yap AS
  TITLE     Anillin Promotes Cell Contractility by Cyclic Resetting of RhoA 
            Residence Kinetics
  JOURNAL   Dev Cell. 2019 Jun 17;49(6):894-906.e12. doi: 
            10.1016/j.devcel.2019.04.031. Epub 2019 May 16.
  PUBMED    31105010
REFERENCE   2  (bases 1 to 10397)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2019.04.031"; journalName: "Dev Cell"; date: 
            "2019-06-17- 17"; volume: "49"; issue: "6"; pages: "894-906.e12"
COMMENT     Sequence Label: PLL5.0-Anillin ShRNA-Cherry-Anillin deltaActin...
FEATURES             Location/Qualifiers
     source          1..10397
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(59..78)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        250..629
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        631..829
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             847..1027
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1074..1199
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1696..1929
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2114..2158
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2456..2573
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2628..2940
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for mouse U6 snRNA (Das 
                     et al., 1988)"
     primer_bind     2859..2879
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     primer_bind     3003..3019
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3061..3094)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     enhancer        3174..3477
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3478..3681
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      3710..3719
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3716..4423
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3861..3879)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4129..4148
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    complement(7211..7244)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    7300..7886
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7353..7373)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7771..7782)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7889..7905)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     LTR             8415..8595
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(8657..9245)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8737..8756)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9416..10207,10208..10276))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10039..10058
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10277..10381)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtgccacctg acgtcgacgg atcgggagat ctcccgatcc cctatggtgc actctcagta
       61 caatctgctc tgatgccgca tagttaagcc agtatctgct ccctgcttgt gtgttggagg
      121 tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt
      181 gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat
      241 atacgcgttg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag
      301 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
      361 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
      421 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
      481 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
      541 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
      601 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
      661 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
      721 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
      781 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcagc gcgttttgcc
      841 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
      901 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
      961 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     1021 tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc
     1081 gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga
     1141 gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca
     1201 gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa
     1261 agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag
     1321 ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac
     1381 catcccttca gacaggatca gaagaactta gatcattata taatacagta gcaaccctct
     1441 attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg
     1501 aagagcaaaa caaaagtaag accaccgcac agcaagcggc cggccgctga tcttcagacc
     1561 tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa
     1621 aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa
     1681 aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat
     1741 gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca
     1801 gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt
     1861 ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca
     1921 acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg
     1981 gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga
     2041 gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca
     2101 aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg
     2161 gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg
     2221 aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca
     2281 gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc
     2341 cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa
     2401 cggatcggca ctgcgtgcgc caattctgca gacaaatggc agtattcatc cacaatttta
     2461 aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa
     2521 cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt
     2581 attacaggga cagcagagat ccagtttggt tagtaccgcg ctctagagat ccgacgcgcc
     2641 atctctaggc ccgcgccggc cccctcgcac agacttgtgg gagaagctcg gctactcccc
     2701 tgccccggtt aatttgcata taatatttcc tagtaactat agaggcttaa tgtgcgataa
     2761 aagacagata atctgttctt tttaatacta gctacatttt acatgatagg cttggatttc
     2821 tataagagat acaaatacta aattattatt ttaaaaaaca gcacaaaagg aaactcaccc
     2881 taactgtaaa gtaattgtgt gttttgagac tataaatatc ccttggagaa aagccttgtt
     2941 tcggcgatgc ctctttgaat aaattcaaga gatttattca aagaggcatc gccatttttt
     3001 ctcgaggtcg acggtatcga taagctcgct tcacgagatt ccagcaggtc gagggaccta
     3061 ataacttcgt atagcataca ttatacgaag ttatattaag ggttccaagc ttaagcggcc
     3121 gcatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
     3181 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
     3241 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
     3301 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
     3361 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
     3421 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg
     3481 atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca
     3541 agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt
     3601 ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg
     3661 gaggtctata taagcagagc tggtttagtg aaccgtcaga tccgctagcg ccaccatggt
     3721 gagcaagggc gaggaggata acatggccat catcaaggag ttcatgcgct tcaaggtgca
     3781 catggagggc tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc
     3841 ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc
     3901 ctgggacatc ctgtcccctc agttcatgta cggctccaag gcctacgtga agcaccccgc
     3961 cgacatcccc gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat
     4021 gaacttcgag gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga
     4081 gttcatctac aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca
     4141 gaagaagacc atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct
     4201 gaagggcgag atcaagcaga ggctgaagct gaaggacggc ggccactacg acgctgaggt
     4261 caagaccacc tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaacat
     4321 caagttggac atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc
     4381 cgagggccgc cactccaccg gcggcatgga cgagctgtac aagccgcgga tggatccgtt
     4441 tacggagaaa ctgctggagc gaacccgtgc caggcgagag aatcttcaga gaaaaatggc
     4501 tgagaggccc acagcagctc caaggtctat gactcatgct aagcgagcta gacagccact
     4561 ttcagaagca agtaaccagc agcccctctc tggtggtgaa gagaaatctt gtacaaaacc
     4621 atcgccatca aaaaaacgct gttctgacaa cactgaagta gaagtttcta acttggaaaa
     4681 taaacaacca gttgagtcga catctgcaaa atcttgttct ccaagtcctg tgtctcctca
     4741 ggtgcagcca caagcagcag ataccatcag tgattctgtt gctgtcccgg catcactgct
     4801 gggcatgagg agagggctga actcaagatt ggaagcaact gcagcctcct cagttaaaac
     4861 acgtatgcaa aaacttgcag agcaacggcg ccgttgggat aatgatgata tgacagatga
     4921 cattcctgaa agctcactct tctcaccaat gccatcagag gaaaaggctg cttcccctcc
     4981 cagacctctg ctttcaaatg cctcggcaac tccagttggc agaaggggcc gtctggccaa
     5041 tcttgctgca actatttgct cctgggaaga tgatgtaaat cactcatttg caaaacaaaa
     5101 cagtgtacaa gaacagcctg gtaccgcttg tttatccaaa ttttcctctg caagtggagc
     5161 atctgctagg atcaatagca cccatttagc acaacagctc aagcaggaac gtcaaaaaga
     5221 actagcatgt cttcgtggcc gatttgacaa gggcaatata tggagtgcag aaaaaggcgg
     5281 aaactcaaaa agcaaacaac tagaaaccaa acaggaaact cactgtcaga gcactcccct
     5341 caaaaaacac caaggtgttt caaaaactca gtcacttcca gtaacagaaa aggtgaccga
     5401 aaaccagata ccagccaaaa attctagtac agaacctaaa gaagtgatac gtgaaattga
     5461 gatgagtgtg gatgatgatg atatcaatag ttcgaaagta attaatgacc tcttcagtga
     5521 tgtcctagag gaaggtgaac tagatatgga gaagagccaa gaggagatgg atcaagcatt
     5581 agcagaaagc agcgaagaac aggaagatgc actgaatatc tcctcaatgt ctttacttgc
     5641 accattggca caaacagttg gtgtggtaag tccagagagt ttagtgtcca cacctagact
     5701 ggaattgaaa gacaccagca gaagtgatga aagtccaaaa ccaggaaaat tccaaagaac
     5761 tcgtgtccct cgagctgaat ctggtgatag ccttggttct gaagatcgtg atcttcttta
     5821 cagcattgat gcatatagat ctcaaagatt caaagaaaca gaacgtccat caataaagca
     5881 ggtgattgtt cggaaggaag atgttacttc aaaactggat gaaaaaaata atgcctttcc
     5941 ttgtcaagtt aatatcaaac agaaaatgca ggaactcaat aacgaaataa atatgcaaca
     6001 gacagtgatc tatcaagcta gccaggctct taactgctgt gttgatgaag aacatggaaa
     6061 agggtcccta gaagaagctg aagcagaaag acttcttcta attgcaactg ggaagagaac
     6121 acttttgatt gatgaattga ataaattgaa gaacgaagga cctcagagga agaataaggc
     6181 tagtccccaa agtgaattta tgccatccaa aggatcagtt actttgtcag aaatccgctt
     6241 gcctctaaaa gcagattttg tctgcagtac ggttcagaaa ccagatgcag caaattacta
     6301 ttacttaatt atactaaaag caggagctga aaatatggta gccacaccat tagcaagtac
     6361 ttcaaactct cttaacggtg atgctctgac attcactact acatttactc tgcaagatgt
     6421 atccaatgac tttgaaataa atattgaagt ttacagcttg gtgcaaaaga aagatccctc
     6481 aggccttgat aagaagaaaa aaacatccaa gtccaaggct attactccaa agcgactcct
     6541 cacatctata accacaaaaa gcaacattca ttcttcagtc atggccagtc caggaggtct
     6601 tagtgctgtg cgaaccagca acttcgccct tgttggatct tacacattat cattgtcttc
     6661 agtaggaaat actaagtttg ttctggacaa ggtccccttt ttatcttctt tggaaggtca
     6721 tatttattta aaaataaaat gtcaagtgaa ttccagtgtt gaagaaagag gttttctaac
     6781 catatttgaa gatgttagtg gttttggtgc ctggcatcga agatggtgtg ttctttctgg
     6841 aaactgtata tcttattgga cttatccaga tgatgagaaa cgcaagaatc ccataggaag
     6901 gataaatctg gctaattgta ccagtcgtca gatagaacca gccaacagag aattttgtgc
     6961 aagacgcaac acttttgaat taattactgt ccgaccacaa agagaagatg accgagagac
     7021 tcttgtcagc caatgcaggg acacactctg tgttaccaag aactggctgt ctgcagatac
     7081 taaagaagag cgggatctct ggatgcaaaa actcaatcaa gttcttgttg atattcgcct
     7141 ctggcaacct gatgcttgct acaaacctat tggaaagcct taacctgcag gcaattcgtc
     7201 gagggaccta ataacttcgt atagcataca ttatacgaag ttatacatgt ttaagggttc
     7261 cggttccact aggtacaatt cgatatcaag cttatcgata atcaacctct ggattacaaa
     7321 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     7381 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     7441 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     7501 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     7561 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     7621 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     7681 gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt
     7741 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     7801 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg
     7861 gatctccctt tgggccgcct ccccgcatcg ataccgtcga cctcgatcga gacctagaaa
     7921 aacatggagc aatcacaagt agcaatacag cagctaccaa tgctgattgt gcctggctag
     7981 aagcacaaga ggaggaggag gtgggttttc cagtcacacc tcaggtacct ttaagaccaa
     8041 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
     8101 ggctaattca ctcccaacga agacaagata tccttgatct gtggatctac cacacacaag
     8161 gctacttccc tgattggcag aactacacac cagggccagg gatcagatat ccactgacct
     8221 ttggatggtg ctacaagcta gtaccagttg agcaagagaa ggtagaagaa gccaatgaag
     8281 gagagaacac ccgcttgtta caccctgtga gcctgcatgg gatggatgac ccggagagag
     8341 aagtattaga gtggaggttt gacagccgcc tagcatttca tcacatggcc cgagagctgc
     8401 atccggactg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     8461 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     8521 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     8581 ggaaaatctc tagcagcatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     8641 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     8701 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     8761 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     8821 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     8881 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     8941 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     9001 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     9061 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     9121 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     9181 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     9241 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     9301 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     9361 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     9421 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     9481 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     9541 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     9601 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     9661 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     9721 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     9781 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     9841 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     9901 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     9961 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
    10021 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
    10081 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
    10141 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
    10201 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
    10261 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
    10321 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
    10381 gcacatttcc ccgaaaa
//
