LOCUS       sequence-372978-       10607 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of shRNA targeting Anillin + reconstitution of
            Anillin delta Myosin binding domain .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10607)
  AUTHORS   Budnar S, Husain KB, Gomez GA, Naghibosadat M, Varma A, Verma S, 
            Hamilton NA, Morris RG, Yap AS
  TITLE     Anillin Promotes Cell Contractility by Cyclic Resetting of RhoA 
            Residence Kinetics
  JOURNAL   Dev Cell. 2019 Jun 17;49(6):894-906.e12. doi: 
            10.1016/j.devcel.2019.04.031. Epub 2019 May 16.
  PUBMED    31105010
REFERENCE   2  (bases 1 to 10607)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2019.04.031"; journalName: "Dev Cell"; date: 
            "2019-06-17- 17"; volume: "49"; issue: "6"; pages: "894-906.e12"
COMMENT     Sequence Label: PLL5.0-Anillin ShRNA-Cherry-Anillin deltaMyosi...
FEATURES             Location/Qualifiers
     source          1..10607
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(69..88)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        260..639
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        641..839
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             857..1037
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1084..1209
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1706..1939
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2124..2168
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2466..2583
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2638..2950
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for mouse U6 snRNA (Das 
                     et al., 1988)"
     primer_bind     2869..2889
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     primer_bind     3013..3029
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3071..3104)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     enhancer        3184..3487
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3488..3691
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      3720..3729
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3726..4433
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3871..3889)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4139..4158
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    complement(7431..7464)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    7520..8106
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7573..7593)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7991..8002)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8109..8125)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     LTR             8635..8815
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(8877..9465)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8957..8976)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9636..10427,10428..10496))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10259..10278
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10497..10601)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tccccgaaaa gtgccacctg acgtcgacgg atcgggagat ctcccgatcc cctatggtgc
       61 actctcagta caatctgctc tgatgccgca tagttaagcc agtatctgct ccctgcttgt
      121 gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg
      181 accgacaatt gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta
      241 cgggccagat atacgcgttg acattgatta ttgactagtt attaatagta atcaattacg
      301 gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc
      361 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc
      421 atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact
      481 gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat
      541 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact
      601 tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac
      661 atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac
      721 gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac
      781 tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcagc
      841 gcgttttgcc tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
      901 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
      961 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     1021 gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag
     1081 gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg
     1141 cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc
     1201 gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg
     1261 ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa
     1321 cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga
     1381 cagctacaac catcccttca gacaggatca gaagaactta gatcattata taatacagta
     1441 gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac
     1501 aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc cggccgctga
     1561 tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata
     1621 aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc
     1681 agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag
     1741 gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg
     1801 gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc
     1861 aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc
     1921 taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg
     1981 ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga
     2041 cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg
     2101 aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg
     2161 caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa
     2221 tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata
     2281 gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac
     2341 ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc
     2401 gattagtgaa cggatcggca ctgcgtgcgc caattctgca gacaaatggc agtattcatc
     2461 cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac
     2521 ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat
     2581 tttcgggttt attacaggga cagcagagat ccagtttggt tagtaccgcg ctctagagat
     2641 ccgacgcgcc atctctaggc ccgcgccggc cccctcgcac agacttgtgg gagaagctcg
     2701 gctactcccc tgccccggtt aatttgcata taatatttcc tagtaactat agaggcttaa
     2761 tgtgcgataa aagacagata atctgttctt tttaatacta gctacatttt acatgatagg
     2821 cttggatttc tataagagat acaaatacta aattattatt ttaaaaaaca gcacaaaagg
     2881 aaactcaccc taactgtaaa gtaattgtgt gttttgagac tataaatatc ccttggagaa
     2941 aagccttgtt tcggcgatgc ctctttgaat aaattcaaga gatttattca aagaggcatc
     3001 gccatttttt ctcgaggtcg acggtatcga taagctcgct tcacgagatt ccagcaggtc
     3061 gagggaccta ataacttcgt atagcataca ttatacgaag ttatattaag ggttccaagc
     3121 ttaagcggcc gcatagtaat caattacggg gtcattagtt catagcccat atatggagtt
     3181 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
     3241 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
     3301 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
     3361 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
     3421 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
     3481 taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg
     3541 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca
     3601 acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg
     3661 tgtacggtgg gaggtctata taagcagagc tggtttagtg aaccgtcaga tccgctagcg
     3721 ccaccatggt gagcaagggc gaggaggata acatggccat catcaaggag ttcatgcgct
     3781 tcaaggtgca catggagggc tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg
     3841 agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggtggccccc
     3901 tgcccttcgc ctgggacatc ctgtcccctc agttcatgta cggctccaag gcctacgtga
     3961 agcaccccgc cgacatcccc gactacttga agctgtcctt ccccgagggc ttcaagtggg
     4021 agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt gacccaggac tcctccctgc
     4081 aggacggcga gttcatctac aaggtgaagc tgcgcggcac caacttcccc tccgacggcc
     4141 ccgtaatgca gaagaagacc atgggctggg aggcctcctc cgagcggatg taccccgagg
     4201 acggcgccct gaagggcgag atcaagcaga ggctgaagct gaaggacggc ggccactacg
     4261 acgctgaggt caagaccacc tacaaggcca agaagcccgt gcagctgccc ggcgcctaca
     4321 acgtcaacat caagttggac atcacctccc acaacgagga ctacaccatc gtggaacagt
     4381 acgaacgcgc cgagggccgc cactccaccg gcggcatgga cgagctgtac aagccgcgga
     4441 tggatccgtt tacggagaaa ctgctggagc gaacccgtgc caggcgagag aatcttcaga
     4501 gaaaaatggc tgagaggccc acagcagctc caaggtctat gactcatgct aagcgagcta
     4561 gacagccact ttcagaagca agtaaccagc agcccctctc tggtggtgaa gagaaatctt
     4621 gtacaaaacc atcgccatca aaaaaacgct gttctgacaa cactgaagta gaagtttcta
     4681 acttggaaaa taaacaacca gttgagtcga catctgcaaa atcttgttct ccaagtcctg
     4741 tgtctcctca ggtgcagcca caagcagcag ataccatcag tgattctgtt gctgtcccgg
     4801 catcactgct gggcatgagg agagggctga actcaagatt ggaagcaact gcagcctcct
     4861 cagttaaaac acgtatgcaa aaacttgcaa gcagtgttaa gcaggaagct acattctgtt
     4921 cccaaaggga tggcgacgca agcttaaaca aggccctatc ctcaagtgct gctgatgcgt
     4981 ctttggttaa tgcctcaatt tccagctctg tgaaagctac ttctccagtg aaatctacta
     5041 catctatcac tgatgctaaa agttgtgagg gacaaaatcc tgagctactt ccaaaaactc
     5101 ctattagtcc tctgaaaacg ggggtatcga aaccaattgt gaagtcaact ttatcccaga
     5161 cagttccatc caagggagaa ttaagtagag aaatttgtct gcaatctcaa tctaaagaca
     5221 aatctacgac accaggagga acaggaatta agcctttcct ggaacgcttt ggagagcgtt
     5281 gtcaagaaca tagcaaagaa agtccagctc gtagcacacc ccacagaacc cccattatta
     5341 ctccaaatac aaaggccatc caagaaaaat tattcaagca agacacatct tcatctacta
     5401 cccatttagc acaacagctc aagcaggaac gtcaaaaaga actagcatgt cttcgtggcc
     5461 gatttgacaa gggcaatata tggagtgcag aaaaaggcgg aaactcaaaa agcaaacaac
     5521 tagaaaccaa acaggaaact cactgtcaga gcactcccct caaaaaacac caaggtgttt
     5581 caaaaactca gtcacttcca gtaacagaaa aggtgaccga aaaccagata ccagccaaaa
     5641 attctagtac agaacctaaa gaagtgatac gtgaaattga gatgagtgtg gatgatgatg
     5701 atatcaatag ttcgaaagta attaatgacc tcttcagtga tgtcctagag gaaggtgaac
     5761 tagatatgga gaagagccaa gaggagatgg atcaagcatt agcagaaagc agcgaagaac
     5821 aggaagatgc actgaatatc tcctcaatgt ctttacttgc accattggca caaacagttg
     5881 gtgtggtaag tccagagagt ttagtgtcca cacctagact ggaattgaaa gacaccagca
     5941 gaagtgatga aagtccaaaa ccaggaaaat tccaaagaac tcgtgtccct cgagctgaat
     6001 ctggtgatag ccttggttct gaagatcgtg atcttcttta cagcattgat gcatatagat
     6061 ctcaaagatt caaagaaaca gaacgtccat caataaagca ggtgattgtt cggaaggaag
     6121 atgttacttc aaaactggat gaaaaaaata atgcctttcc ttgtcaagtt aatatcaaac
     6181 agaaaatgca ggaactcaat aacgaaataa atatgcaaca gacagtgatc tatcaagcta
     6241 gccaggctct taactgctgt gttgatgaag aacatggaaa agggtcccta gaagaagctg
     6301 aagcagaaag acttcttcta attgcaactg ggaagagaac acttttgatt gatgaattga
     6361 ataaattgaa gaacgaagga cctcagagga agaataaggc tagtccccaa agtgaattta
     6421 tgccatccaa aggatcagtt actttgtcag aaatccgctt gcctctaaaa gcagattttg
     6481 tctgcagtac ggttcagaaa ccagatgcag caaattacta ttacttaatt atactaaaag
     6541 caggagctga aaatatggta gccacaccat tagcaagtac ttcaaactct cttaacggtg
     6601 atgctctgac attcactact acatttactc tgcaagatgt atccaatgac tttgaaataa
     6661 atattgaagt ttacagcttg gtgcaaaaga aagatccctc aggccttgat aagaagaaaa
     6721 aaacatccaa gtccaaggct attactccaa agcgactcct cacatctata accacaaaaa
     6781 gcaacattca ttcttcagtc atggccagtc caggaggtct tagtgctgtg cgaaccagca
     6841 acttcgccct tgttggatct tacacattat cattgtcttc agtaggaaat actaagtttg
     6901 ttctggacaa ggtccccttt ttatcttctt tggaaggtca tatttattta aaaataaaat
     6961 gtcaagtgaa ttccagtgtt gaagaaagag gttttctaac catatttgaa gatgttagtg
     7021 gttttggtgc ctggcatcga agatggtgtg ttctttctgg aaactgtata tcttattgga
     7081 cttatccaga tgatgagaaa cgcaagaatc ccataggaag gataaatctg gctaattgta
     7141 ccagtcgtca gatagaacca gccaacagag aattttgtgc aagacgcaac acttttgaat
     7201 taattactgt ccgaccacaa agagaagatg accgagagac tcttgtcagc caatgcaggg
     7261 acacactctg tgttaccaag aactggctgt ctgcagatac taaagaagag cgggatctct
     7321 ggatgcaaaa actcaatcaa gttcttgttg atattcgcct ctggcaacct gatgcttgct
     7381 acaaacctat tggaaagcct taacctgcag gcaattcgtc gagggaccta ataacttcgt
     7441 atagcataca ttatacgaag ttatacatgt ttaagggttc cggttccact aggtacaatt
     7501 cgatatcaag cttatcgata atcaacctct ggattacaaa atttgtgaaa gattgactgg
     7561 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     7621 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     7681 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     7741 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     7801 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     7861 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc
     7921 gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     7981 ctacgtccct tcggccctca atccagcgga ccttccttcc cggcctgctg ccggctctgc
     8041 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
     8101 ccccgcatcg ataccgtcga cctcgatcga gacctagaaa aacatggagc aatcacaagt
     8161 agcaatacag cagctaccaa tgctgattgt gcctggctag aagcacaaga ggaggaggag
     8221 gtgggttttc cagtcacacc tcaggtacct ttaagaccaa tgacttacaa ggcagctgta
     8281 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga
     8341 agacaagata tccttgatct gtggatctac cacacacaag gctacttccc tgattggcag
     8401 aactacacac cagggccagg gatcagatat ccactgacct ttggatggtg ctacaagcta
     8461 gtaccagttg agcaagagaa ggtagaagaa gccaatgaag gagagaacac ccgcttgtta
     8521 caccctgtga gcctgcatgg gatggatgac ccggagagag aagtattaga gtggaggttt
     8581 gacagccgcc tagcatttca tcacatggcc cgagagctgc atccggactg tactgggtct
     8641 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     8701 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     8761 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagcatg
     8821 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     8881 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     8941 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     9001 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     9061 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     9121 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     9181 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     9241 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     9301 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     9361 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     9421 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     9481 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     9541 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     9601 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     9661 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     9721 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     9781 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     9841 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     9901 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     9961 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
    10021 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
    10081 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
    10141 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
    10201 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
    10261 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
    10321 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
    10381 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
    10441 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
    10501 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
    10561 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatt
//
