LOCUS       sequence-372992-        8851 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression plasmid of human IRF8 fused with FKBP12G36V 
            for dTAG-mediated degradation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8851)
  AUTHORS   Cao Z, Budinich KA, Huang H, Ren D, Lu B, Zhang Z, Chen Q, Zhou Y, 
            Huang YH, Alikarami F, Kingsley MC, Lenard AK, Wakabayashi A, 
            Khandros E, Bailis W, Qi J, Carroll MP, Blobel GA, Faryabi RB, Bernt
            KM, Berger SL, Shi J
  TITLE     ZMYND8-regulated IRF8 transcription axis is an acute myeloid 
            leukemia dependency.
  JOURNAL   Mol Cell. 2021 Sep 2;81(17):3604-3622.e10. doi: 
            10.1016/j.molcel.2021.07.018. Epub 2021 Aug 5.
  PUBMED    34358447
REFERENCE   2  (bases 1 to 8851)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2021.07.018"; journalName: "Mol Cell"; date: 
            "2021-09-2- 2"; volume: "81"; issue: "17"; pages: "3604-3622.e10"
COMMENT     Sequence Label: dTAG-IRF8
FEATURES             Location/Qualifiers
     source          1..8851
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1723..1840
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1909..2120
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      2140..2149
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2146..2148,2149..2469)
                     /codon_start=1
                     /product="F36V mutant of FK506-binding protein FKBP12"
                     /label=FKBP-V
                     /note="binds synthetic ligands that do not bind wild-type 
                     FKBP"
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKLE"
     CDS             2476..2502
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             2476..2502
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2506..2532
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3862..3918
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3913..4713
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="GPMIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQG
                     RPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEE
                     HQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQD
                     IALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3973..3992)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4583..4602
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    4729..5317
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4782..4802)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5200..5211)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5389..5622
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5700..5821
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5737..5756)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5791..5810
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5840..5975
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5995..6014)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5996..6014)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6024..6041)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6024..6040)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6033..6055)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6182..6637
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6269..6288)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6479..6500
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6663..6767
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6768..6836,6837..7628)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6986..7005)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7799..8387
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8288..8307
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8541..8558
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8675..8696
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8711..8716,8717..8734,8735..8741)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8749..8765
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8754..8776
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8773..8789
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8773..8789
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8808..8828
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8810..8828
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccagaccac aattttaaaa gaaaaggggg
     1741 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     1801 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     1861 cagagatcca ctttggcgcc ggctcgagtg gctccggtgc ccgtcagtgg gcagagcgca
     1921 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga
     1981 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg
     2041 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg
     2101 ggtttgccgc cagaacacag gtgtcgtgac gcgggatccg ccaccatggg agtgcaggtg
     2161 gaaaccatct ccccaggaga cgggcgcacc ttccccaagc gcggccagac ctgcgtggtg
     2221 cactacaccg ggatgcttga agatggaaag aaagttgatt cctcccggga cagaaacaag
     2281 ccctttaagt ttatgctagg caagcaggag gtgatccgag gctgggaaga aggggttgcc
     2341 cagatgagtg tgggtcagag agccaaactg actatatctc cagattatgc ctatggtgcc
     2401 actgggcacc caggcatcat cccaccacat gccactctcg tcttcgatgt ggagcttcta
     2461 aaactggaag gcggctaccc ctacgacgtg cccgactacg ccggctatcc gtatgatgtc
     2521 ccggactatg caggcagcat ggcaggtgtc ccggagtccc tgaatctgat gtgtgaccgg
     2581 aatggtggtc ggcggcttcg acagtggctg atcgagcaga tcgattcatc gatgtaccca
     2641 ggactgattt gggagaatga ggagaagagc atgttccgga tcccttggaa acacgctggc
     2701 aagcaagatt ataatcagga agtggatgcc tccattttta aggcctgggc agtttttaaa
     2761 gggaagttta aagaagggga caaagctgaa ccagccactt ggaagacgag gttacgctgt
     2821 gctttgaata agagcccaga ttttgaggaa gtgacggacc ggtcccaact ggacatttcc
     2881 gagccataca aagtttaccg aattgttcct gaggaagagc aaaaatgcaa actaggcgtg
     2941 gcaactgctg gctgcgtgaa tgaagttaca gagatggagt gcggtcgctc tgaaatcgac
     3001 gagctgatca aggagccttc tgtggacgat tacatgggga tgatcaaaag gagcccttcc
     3061 ccgccggagg cctgtcggag tcagctcctt ccagactggt gggcgcagca gcccagcaca
     3121 ggcgtgccgc tggtgacggg gtacaccacc tacgacgcgc accattcagc attctcccag
     3181 atggtgatca gcttctacta tgggggcaag ctggtgggcc aggccaccac cacctgcccc
     3241 gagggctgcc gcctgtccct gagccagcct gggctgcccg gcaccaagct gtatgggccc
     3301 gagggcctgg agctggtgcg cttcccgccg gccgacgcca tccccagcga gcgacagagg
     3361 caggtgacgc ggaagctgtt cgggcacctg gagcgcgggg tgctgctgca cagcagccgg
     3421 cagggcgtgt tcgtcaagcg gctgtgccag ggccgcgtgt tctgcagcgg caacgccgtg
     3481 gtgtgcaaag gcaggcccaa caagctggag cgtgatgagg tggtccaggt cttcgacacc
     3541 agccagttct tccgagagct gcagcagttc tataacagcc agggccggct tcctgacggc
     3601 agggtggtgc tgtgctttgg ggaagagttt ccggatatgg cccccttgcg ctccaaactc
     3661 attctcgtgc agattgagca gctgtatgtc cggcaactgg cagaagaggc tgggaagagc
     3721 tgtggagccg gctctgtgat gcaggccccc gaggagccgc cgccagacca ggtcttccgg
     3781 atgtttccag atatttgtgc ctcacaccag agatcatttt tcagagaaaa ccaacagatc
     3841 accgtcgcta gcggcagcgg cgccaccaac ttcagcctgc tgaagcaggc cggcgacgtg
     3901 gaggagaacc ccggccccat gattgaacaa gatggattgc acgcaggttc tccggccgct
     3961 tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc
     4021 gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc
     4081 ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc
     4141 gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg
     4201 ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc
     4261 atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac
     4321 caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat
     4381 caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc
     4441 aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg
     4501 aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg
     4561 gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc
     4621 gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc
     4681 gccttctatc gccttcttga cgagttcttc tgaacgcgtt aagtcgacaa tcaacctctg
     4741 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     4801 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     4861 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     4921 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     4981 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     5041 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     5101 aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc
     5161 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     5221 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     5281 cagacgagtc ggatctccct ttgggccgcc tccccgcgtc gactttaaga ccaatgactt
     5341 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa
     5401 ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc
     5461 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     5521 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     5581 gatccctcag acccttttag tcagtgtgga aaatctctag cagtacgtat agtagttcat
     5641 gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga
     5701 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     5761 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     5821 atcatgtctg gctctagcta tcccgcccct aactccgccc agttccgccc attctccgcc
     5881 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     5941 attccagaag tagtgaggag gcttttttgg aggcctaggg acgtacccaa ttcgccctat
     6001 agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac
     6061 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
     6121 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
     6181 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     6241 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     6301 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     6361 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     6421 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     6481 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     6541 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     6601 aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg
     6661 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     6721 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     6781 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     6841 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     6901 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     6961 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     7021 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     7081 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     7141 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     7201 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     7261 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     7321 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     7381 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     7441 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     7501 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     7561 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     7621 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     7681 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     7741 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     7801 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     7861 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     7921 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     7981 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     8041 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     8101 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     8161 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     8221 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     8281 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     8341 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     8401 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     8461 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     8521 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     8581 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     8641 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
     8701 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
     8761 aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc
     8821 actaaaggga acaaaagctg gagctgcaag c
//
