LOCUS       sequence-372997-       11329 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  An improved agroinfection-compatible Tobacco mosaic virus 
            (TMV)-based transient expression vector that lacks the TMV CP gene 
            coding sequence and expresses GFP..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11329)
  AUTHORS   Lindbo JA
  TITLE     TRBO: a high-efficiency tobacco mosaic virus RNA-based 
            overexpression vector.
  JOURNAL   Plant Physiol. 2007 Dec;145(4):1232-40. Epub 2007 Aug 24.
  PUBMED    17720752
REFERENCE   2  (bases 1 to 11329)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2007-12"; volume: "145(4)"; pages: "1232-40. Epub 
            2007 Aug 24"
COMMENT     Sequence Label: pJL-TRBO-G
FEATURES             Location/Qualifiers
     source          1..11329
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(157..1305)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MAQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(1604..2398)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHLERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      2607..3242
                     /label=oriV
                     /note="incP origin of replication"
     misc_feature    3322..3346
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     primer_bind     3426..3442
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     3427..3443
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3874..4219
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     4162..4182
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     misc_feature    4228..4283
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             join(9981..9983,9984..9986,9987..10700)
                     /codon_start=1
                     /product="Cycle 3 GFP (Crameri et al., 1996)"
                     /label=Cycle 3 GFP
                     /note="mammalian codon-optimized"
                     /translation="MASKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(10014..10042)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     10637..10657
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     misc_feature    join(11307..11329,1..2)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 acctaagaga aaagagcgtt tattagaata atcggatatt taaaagggcg tgaaaaggtt
       61 tatccgttcg tccatttgta tgtgcatgcc aaccacagga gatctcagta aagcgctggc
      121 tgctgaaccc ccagccggaa ctgaccccac aaggccctag cgtttgcaat gcaccaggtc
      181 atcattgacc caggcgtgtt ccaccaggcc gctgcctcgc aactcttcgc aggcttcgcc
      241 gacctgctcg cgccacttct tcacgcgggt ggaatccgat ccgcacatga ggcggaaggt
      301 ttccagcttg agcgggtacg gctcccggtg cgagctgaaa tagtcgaaca tccgtcgggc
      361 cgtcggcgac agcttgcggt acttctccca tatgaatttc gtgtagtggt cgccagcaaa
      421 cagcacgacg atttcctcgt cgatcaggac ctggcaacgg gacgttttct tgccacggtc
      481 caggacgcgg aagcggtgca gcagcgacac cgattccagg tgcccaacgc ggtcggacgt
      541 gaagcccatc gccgtcgcct gtaggcgcga caggcattcc tcggccttcg tgtaataccg
      601 gccattgatc gaccagccca ggtcctggca aagctcgtag aacgtgaagg tgatcggctc
      661 gccgataggg gtgcgcttcg cgtactccaa cacctgctgc cacaccagtt cgtcatcgtc
      721 ggcccgcagc tcgacgccgg tgtaggtgat cttcacgtcc ttgttgacgt ggaaaatgac
      781 cttgttttgc agcgcctcgc gcgggatttt cttgttgcgc gtggtgaaca gggcagagcg
      841 ggccgtgtcg tttggcatcg ctcgcatcgt gtccggccac ggcgcaatat cgaacaagga
      901 aagctgcatt tccttgatct gctgcttcgt gtgtttcagc aacgcggcct gcttggcctc
      961 gctgacctgt tttgccaggt cctcgccggc ggtttttcgc ttcttggtcg tcatagttcc
     1021 tcgcgtgtcg atggtcatcg acttcgccaa acctgccgcc tcctgttcga gacgacgcga
     1081 acgctccacg gcggccgatg gcgcgggcag ggcaggggga gccagttgca cgctgtcgcg
     1141 ctcgatcttg gccgtagctt gctgggccat cgagccgacg gactggaagg tttcgcgggg
     1201 cgcacgcatg acggtgcggc ttgcgatggt ttcggcatcc tcggcggaaa accccgcgtc
     1261 gatcagttct tgcctgtatg ccttccggtc aaacgtccga ttcattcacc ctccttgcgg
     1321 gattgccccg actcacgccg gggcaatgtg cccttattcc tgatttgacc cgcctggtgc
     1381 cttggtgtcc agataatcca ccttatcggc aatgaagtcg gtcccgtaga ccgtctggcc
     1441 gtccttctcg tacttggtat tccgaatctt gccctgcacg aataccagcg accccttgcc
     1501 caaatacttg ccgtgggcct cggcctgaga gccaaaacac ttgatgcgga agaagtcggt
     1561 gcgctcctgc ttgtcgccgg catcgttgcg ccacatctag gtactaaaac aattcatcca
     1621 gtaaaatata atattttatt ttctcccaat caggcttgat ccccagtaag tcaaaaaata
     1681 gctcgacata ctgttcttcc ccgatatcct ccctgatcga ccggacgcag aaggcaatgt
     1741 cataccactt gtccgccctg ccgcttctcc caagatcaat aaagccactt actttgccat
     1801 ctttcacaaa gatgttgctg tctcccaggt cgccgtggga aaagacaagt tcctcttcgg
     1861 gcttttccgt ctttaaaaaa tcatacagct cgcgcggatc tttaaatggg gtgtcttctt
     1921 cccagttttc gcaatccaca tcggccagat cgttattcag taagtaatcc aattcggcta
     1981 agcggctgtc taagctattc gtatagggac aatccgatat gtcgatggag tgaaagagcc
     2041 tgatgcactc cgcatacagc tcgataatct tttcagggct ttgttcatct tcatactctt
     2101 ccgagcaaag gacgccatcg gcctcactca tgagcagatt gctccagcca tcatgccgtt
     2161 caaggtgcag gacctttgga acaggcagct ttccttccag ccatagcatc atgtcctttt
     2221 cccgttccac atcataggtg gtccctttat accggctgtc cgtcattttt aaatataggt
     2281 tttcattttc tcccaccagc ttatatacct tagcaggaga cattccttcc gtatctttta
     2341 cgcagcggta tttttcgatc agttttttca attccggtga tattctcatt ttagccattt
     2401 attatttcct tcctcttttc tacagtattt aaagataccc caagaagcta attataacaa
     2461 gacgaactcc aattcactgt tccttgcatt ctaaaacttt aaataccaga aaacagcttt
     2521 ttcaaagttg ttttcaaagt tggcgtataa catagtatcg acggagccga ttttgaaacc
     2581 acaattatgg gtgatgctgc aactcgagcg ggccgggagg gttcgagaag ggggggcacc
     2641 ccccttcggc gtgcgcggtc acgcgcacag ggcgcagccc tggttaaaaa caaggtttat
     2701 aaatattggt ttaaaagcag gttaaaagac aggttagcgg tggccgaaaa acgggcggaa
     2761 acccttgcaa atgctggatt ttctgcctgt ggacagcccc tcaaatgtca ataggtgcgc
     2821 ccctcatctg tcagcactct gcccctcaag tgtcaaggat cgcgcccctc atctgtcagt
     2881 agtcgcgccc ctcaagtgtc aataccgcag ggcacttatc cccaggcttg tccacatcat
     2941 ctgtgggaaa ctcgcgtaaa atcaggcgtt ttcgccgatt tgcgaggctg gccagctcca
     3001 cgtcgccggc cgaaatcgag cctgcccctc atctgtcaac gccgcgccgg gtgagtcggc
     3061 ccctcaagtg tcaacgtccg cccctcatct gtcagtgagg gccaagtttt ccgcgaggta
     3121 tccacaacgc cggcggccgg ccgcggtgtc tcgcacacgg cttcgacggc gtttctggcg
     3181 cgtttgcagg gccatagacg gccgccagcc cagcggcgag ggcaaccagc ccggtgagcg
     3241 tctctagtgg actgatgggc tgcctgtatc gagtggtgat tttgtgccga gctgccggtc
     3301 ggggagctgt tggctggctg gtggcaggat atattgtggt gtaaacaaat tgacgcttag
     3361 acaacttaat aacacattgc ggacgttttt aaagtactgg ggtggttttg gtaccgggcc
     3421 ccccctcgag gtcgacggta tcgataagct tgatatcgaa ttcctgcagg tcaacatggt
     3481 ggagcacgac acacttgtct actccaaaaa tatcaaagat acagtctcag aagaccaaag
     3541 ggcaattgag acttttcaac aaagggtaat atccggaaac ctcctcggat tccattgccc
     3601 agctatctgt cactttattg tgaagatagt ggaaaaggaa ggtggctcct acaaatgcca
     3661 tcattgcgat aaaggaaagg ccatcgttga agatgcctct gccgacagtg gtcccaaaga
     3721 tggaccccca cccacgagga gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa
     3781 gcaagtggat tgatgtgata acatggtgga gcacgacaca cttgtctact ccaaaaatat
     3841 caaagataca gtctcagaag accaaagggc aattgagact tttcaacaaa gggtaatatc
     3901 cggaaacctc ctcggattcc attgcccagc tatctgtcac tttattgtga agatagtgga
     3961 aaaggaaggt ggctcctaca aatgccatca ttgcgataaa ggaaaggcca tcgttgaaga
     4021 tgcctctgcc gacagtggtc ccaaagatgg acccccaccc acgaggagca tcgtggaaaa
     4081 agaagacgtt ccaaccacgt cttcaaagca agtggattga tgtgatatct ccactgacgt
     4141 aagggatgac gcacaatccc actatccttc gcaagaccct tcctctatat aaggaagttc
     4201 atttcatttg gagagggtat ttttacaaca attaccaaca acaacaaaca acagacaaca
     4261 ttacaattac tatttacaat tacaatggca tacacacaga cagctaccac atcagctttg
     4321 ctggacactg tccgaggaaa caactccttg gtcaatgatc tagcaaagcg tcgtctttac
     4381 gacacagcgg ttgaagagtt taacgctcgt gaccgcaggc ccaaggtgaa cttttcaaaa
     4441 gtaataagcg aggagcagac gcttattgct acccgggcgt atccagaatt ccaaattaca
     4501 ttttataaca cgcaaaatgc cgtgcattcg cttgcaggtg gattgcgatc tttagaactg
     4561 gaatatctga tgatgcaaat tccctacgga tcattgactt atgacatagg cgggaatttt
     4621 gcatcgcatc tgttcaaggg acgagcatat gtacactgct gcatgcccaa cctggacgtt
     4681 cgagacatca tgcggcacga aggccagaaa gacagtattg aactatacct ttctaggcta
     4741 gagagagggg ggaaaacagt ccccaacttc caaaaggaag catttgacag atacgcagaa
     4801 attcctgaag acgctgtctg tcacaatact ttccagacat gcgaacatca gccgatgcag
     4861 caatcaggca gagtgtatgc cattgcgcta cacagcatat atgacatacc agccgatgag
     4921 ttcggggcgg cactcttgag gaaaaatgtc catacgtgct atgccgcttt ccacttctcc
     4981 gagaacctgc ttcttgaaga ttcatgcgtc aatttggacg aaatcaacgc gtgtttttcg
     5041 cgcgatggag acaagttgac cttttctttt gcatcagaga gtactcttaa ttactgtcat
     5101 agttattcta atattcttaa gtatgtgtgc aaaacttact tcccggcctc taatagagag
     5161 gtttacatga aggagttttt agtcaccaga gttaatacct ggttttgtaa gttttctaga
     5221 atagatactt ttcttttgta caaaggtgtg gcccataaaa gtgtagatag tgagcagttt
     5281 tatactgcaa tggaagacgc atggcattac aaaaagactc ttgcaatgtg caacagcgag
     5341 agaatcctcc ttgaggattc atcatcagtc aattactggt ttcccaaaat gagggatatg
     5401 gtcatcgtac cattattcga catttctttg gagactagta agaggacgcg caaggaagtc
     5461 ttagtgtcca aggatttcgt gttcacagtg cttaaccaca ttcgaacata ccaggcgaaa
     5521 gctcttacat acgcaaatgt tttgtccttc gtcgaatcga ttcgatcgag ggtaatcatt
     5581 aacggtgtga cagcgaggtc cgaatgggat gtggacaaat ctttgttaca atccttgtcc
     5641 atgacgtttt acctgcatac taagcttgcc gttctaaagg atgacttact gattagcaag
     5701 tttagtctcg gttcgaaaac ggtgtgccag catgtgtggg atgagatttc gctggcgttt
     5761 gggaacgcat ttccctccgt gaaagagagg ctcttgaaca ggaaacttat cagagtggca
     5821 ggcgacgcat tagagatcag ggtgcctgat ctatatgtga ccttccacga cagattagtg
     5881 actgagtaca aggcctctgt ggacatgcct gcgcttgaca ttaggaagaa gatggaagaa
     5941 acggaagtga tgtacaatgc actttcagaa ttatcggtgt taagggagtc tgacaaattc
     6001 gatgttgatg ttttttccca gatgtgccaa tctttggaag ttgacccaat gacggcagcg
     6061 aaggttatag tcgcggtcat gagcaatgag agcggtctga ctctcacatt tgaacgacct
     6121 actgaggcga atgttgcgct agctttacag gatcaagaga aggcttcaga aggtgcattg
     6181 gtagttacct caagagaagt tgaagaaccg tccatgaagg gttcgatggc cagaggagag
     6241 ttacaattag ctggtcttgc tggagatcat ccggaatcgt cctattctaa gaacgaggag
     6301 atagagtctt tagagcagtt tcatatggcg acggcagatt cgttaattcg taagcagatg
     6361 agctcgattg tgtacacggg tccgattaaa gttcagcaaa tgaaaaactt tatcgatagc
     6421 ctggtagcat cactatctgc tgcggtgtcg aatctcgtca agatcctcaa agatacagct
     6481 gctattgacc ttgaaacccg tcaaaagttt ggagtcttgg atgttgcatc taggaagtgg
     6541 ttaatcaaac caacggccaa gagtcatgca tggggtgttg ttgaaaccca cgcgaggaag
     6601 tatcatgtgg cgcttttgga atatgatgag cagggtgtgg tgacatgcga tgattggaga
     6661 agagtagctg ttagctctga gtctgttgtt tattccgaca tggcgaaact cagaactctg
     6721 cgcagactgc ttcgaaacgg agaaccgcat gtcagtagcg caaaggttgt tcttgtggac
     6781 ggagttccgg gctgtggaaa aaccaaagaa attctttcca gggttaattt tgatgaagat
     6841 ctaattttag tacctgggaa gcaagccgcg gaaatgatca gaagacgtgc gaattcctca
     6901 gggattattg tggccacgaa ggacaacgtt aaaaccgttg attctttcat gatgaatttt
     6961 gggaaaagca cacgctgtca gttcaagagg ttattcattg atgaagggtt gatgttgcat
     7021 actggttgtg ttaattttct tgtggcgatg tcattgtgcg aaattgcata tgtttacgga
     7081 gacacacagc agattccata catcaataga gtttcaggat tcccgtaccc cgcccatttt
     7141 gccaaattgg aagttgacga ggtggagaca cgcagaacta ctctccgttg tccagccgat
     7201 gtcacacatt atctgaacag gagatatgag ggctttgtca tgagcacttc ttcggttaaa
     7261 aagtctgttt cgcaggagat ggtcggcgga gccgccgtga tcaatccgat ctcaaaaccc
     7321 ttgcatggca agatcctgac ttttacccaa tcggataaag aagctctgct ttcaagaggg
     7381 tattcagatg ttcacactgt gcatgaagtg caaggcgaga catactctga tgtttcacta
     7441 gttaggttaa cccctacacc ggtctccatc attgcaggag acagcccaca tgttttggtc
     7501 gcattgtcaa ggcacacctg ttcgctcaag tactacactg ttgttatgga tcctttagtt
     7561 agtatcatta gagatctaga gaaacttagc tcgtacttgt tagatatgta taaggtcgat
     7621 gcaggaacac aatagcaatt acagattgac tcggtgttca aaggttccaa tctttttgtt
     7681 gcagcgccaa agactggtga tatttctgat atgcagtttt actatgataa gtgtctccca
     7741 ggcaacagca ccatgatgaa taattttgat gctgttacca tgaggttgac tgacatttca
     7801 ttgaatgtca aagattgcat attggatatg tctaagtctg ttgctgcgcc taaggatcaa
     7861 atcaaaccac taatacctat ggtacgaacg gcggcagaaa tgccacgcca gactggacta
     7921 ttggaaaatt tagtggcgat gattaaaaga aactttaacg cacccgagtt gtctggcatc
     7981 attgatattg aaaatactgc atctttggtt gtagataagt tttttgatag ttatttgctt
     8041 aaagaaaaaa gaaaaccaaa taaaaatgtt tctttgttca gtagagagtc tctcaataga
     8101 tggttagaaa agcaggaaca ggtaacaata ggccagctcg cagattttga ttttgtggat
     8161 ttgccagcag ttgatcagta cagacacatg attaaagcac aacccaaaca aaagttggac
     8221 acttcaatcc aaacggagta cccggctttg cagacgattg tgtaccattc aaaaaagatc
     8281 aatgcaatat tcggcccgtt gtttagtgag cttaccaggc aattactgga cagtgttgat
     8341 tcgagcagat ttttgttttt cacaagaaag acaccagcgc agattgagga tttcttcgga
     8401 gatctcgaca gtcatgtgcc gatggatgtc ttggagctgg atatatcaaa atacgacaaa
     8461 tctcagaatg aattccactg tgcagtagaa tacgagatct ggcgaagatt gggtttcgaa
     8521 gacttcttgg gagaagtttg gaaacaaggg catagaaaga ccaccctcaa ggattatacc
     8581 gcaggtataa aaacttgcat ctggtatcaa agaaagagcg gggacgtcac gacgttcatt
     8641 ggaaacactg tgatcattgc tgcatgtttg gcctcgatgc ttccgatgga gaaaataatc
     8701 aaaggagcct tttgcggtga cgatagtctg ctgtactttc caaagggttg tgagtttccg
     8761 gatgtgcaac actccgcgaa tcttatgtgg aattttgaag caaaactgtt taaaaaacag
     8821 tatggatact tttgcggaag atatgtaata catcacgaca gaggatgcat tgtgtattac
     8881 gatcccctaa agttgatctc gaaacttggt gctaaacaca tcaaggattg ggaacacttg
     8941 gaggagttca gaaggtctct ttgtgatgtt gctgtttcgt tgaacaattg tgcgtattac
     9001 acacagttgg acgacgctgt atgggaggtt cataagaccg cccctccagg ttcgtttgtt
     9061 tataaaagtc tggtgaagta tttgtctgat aaagttcttt ttagaagttt gtttatagat
     9121 ggctctagtt gttaaaggaa aagtgaatat caatgagttt atcgacctga caaaaatgga
     9181 gaagatctta ccgtcgatgt ttacccctgt aaagagtgtt atgtgttcca aagttgataa
     9241 aataatggtt catgagaatg agtcattgtc aggggtgaac cttcttaaag gagttaagct
     9301 tattgatagt ggatacgtct gtttagccgg tttggtcgtc acgggcgagt ggaacttgcc
     9361 tgacaattgc agaggaggtg tgagcgtgtg tctggtggac aaaaggatgg aaagagccga
     9421 cgaggccatt ctcggatctt actacacagc agctgcaaag aaaagatttc agttcaaggt
     9481 cgttcccaat tatgctataa ccacccagga cgcgatgaaa aacgtctggc aagttttagt
     9541 taatattaga aatgtgaaga tgtcagcggg tttctgtccg ctttctctgg agtttgtgtc
     9601 ggtgtgtatt gtttatagaa ataatataaa attaggtttg agagagaaga ttacaaacgt
     9661 gagagacgga gggcccatgg aacttacaga agaagtcgtt gatgagttca tggaagatgt
     9721 ccctatgtcg atcaggcttg caaagtttcg atctcgaacc ggaaaaaaga gtgatgtccg
     9781 caaagggaaa aatagtagta gtgatcggtc agtgccgaac aagaactata gaaatgttaa
     9841 ggattttggg ggaatgagtt ttaaaaagaa taatttaatc gatgatgatt cggaggctac
     9901 tgtcgccgaa tcggattcgt tttaaataga tcttacagta tcactactcc atctcagttc
     9961 gtgttcttgt cattaattaa atggctagca aaggagaaga acttttcact ggagttgtcc
    10021 caattcttgt tgaattagat ggtgatgtta atgggcacaa attttctgtc agtggagagg
    10081 gtgaaggtga tgctacatac ggaaagctta cacttaaatt tatttgcact actggaaaac
    10141 tacctgttcc atggccaaca cttgtcacta ctttctctta tggtgttcaa tgcttttccc
    10201 gttatccgga tcatatgaaa cggcatgact ttttcaagag tgccatgccc gaaggttatg
    10261 tacaggaacg cactatatct ttcaaagatg acgggaacta caagacgcgt gctgaagtca
    10321 agtttgaagg tgataccctt gttaatcgta tcgagttaaa aggtattgat tttaaagaag
    10381 atggaaacat tctcggacac aaactcgagt acaactataa ctcacacaat gtatacatca
    10441 cggcagacaa acaaaagaat ggaatcaaag ctaacttcaa aattcgccac aacattgaag
    10501 atggatccgt tcaactagca gaccattatc aacaaaatac tccaattggc gatggccctg
    10561 tccttttacc agacaaccat tacctgtcga cacaatctgc cctttcgaaa gatcccaacg
    10621 aaaagcgtga ccacatggtc cttcttgagt ttgtaactgc tgctgggatt acacatggca
    10681 tggatgagct ctacaaataa actagggcgg ccgcggtcct gcaacttgag gtagtcaaga
    10741 tgcataataa ataacggatt gtgtccgtaa tcacacgtgg tgcgtacgat aacgcatagt
    10801 gtttttccct ccacttaaat cgaagggttg tgtcttggat cgcgcgggtc aaatgtatat
    10861 ggttcatata catccgcagg cacgtaataa agcgaggggt tcgaatcccc ccgttacccc
    10921 cggtaggggc ccaggtaccc ggatgtgttt tccgggctga tgagtccgtg aggacgaaac
    10981 ctggctgcag gcatgcaagc ttggcgtaat catggtcata gcctagagtc cgcaaaaatc
    11041 accagtctct ctctacaaat ctatctctct ctatttttct ccagaataat gtgtgagtag
    11101 ttcccagata agggaattag ggttcttata gggtttcgct catgtgttga gcatataaga
    11161 aacccttagt atgtatttgt atttgtaaaa tacttctatc aataaaattt ctaattccta
    11221 aaaccaaaat ccagtgacct gcagcccggc cgggggatcc actagcagat tgtcgtttcc
    11281 cgccttcagt ttaaactatc agtgtttgac aggatatatt ggcgggtaa
//
