LOCUS       sequence-37300-e         930 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Osborn MJ, Defeo AP, Blazar B, Tolar J
  TITLE     Synthetic Zinc Finger Nuclease Design and Rapid Assembly.
  JOURNAL   Hum Gene Ther. 2011 Jun 13.
  PUBMED    21663559
REFERENCE   2  (bases 1 to 930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Hum Gene 
            Ther. 2011 Jun 13."
COMMENT     Sequence Label: pMJO-7
FEATURES             Location/Qualifiers
     source          1..930
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(7..24)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(34..75)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             complement(97..681)
                     /codon_start=1
                     /product="nonspecific DNA cleavage domain of the FokI 
                     endonuclease (Li et al., 1992)"
                     /label=FokI cleavage domain
                     /note="must dimerize to be catalytically active"
                     /translation="LVKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVM
                     EFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMERY
                     VEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNCNGAVLS
                     VEELLIGGEMIKAGTLTLEEVRRKFNNGEINF"
     CDS             complement(730..750)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(757..822)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             complement(829..882)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 ggtaccatgg tgatggtgat gatgaccggt acgcgtagaa tcgagaccga ggagagggtt
       61 agggataggc ttaccttcga agggccgagc tcaataaaag tttatctcgc cgttattaaa
      121 tttccgtctc acttcctcta aggttaatgt gccggcttta atcatttctc caccaattaa
      181 aagctcttct acactaagaa cagctccatt acaattagtg atatgattta atcgtgtaag
      241 ctgagctttg tagtttcctt taaagtgacc actcacaaat aaaaacttaa attccgttac
      301 agaagatgga tagactttcc accattcatt agggttgaga tgtttgtttc gtgtttgatt
      361 ttcttcgaca tatcgctcca tttcatctgc ttggccaatt ggcagattat aacctccgct
      421 ataagcttta gtatccacga tcacaccgta atcaatagga gatccgacag tataaattgc
      481 tccgtccggt ttccttgatc cacccaaatg tttacctcta tatccataaa ctttcataaa
      541 aaattccatt accttcattt caagaattct atcctgagtg gaatttctgg caatttcaat
      601 taattcaata tattcatgag gcacatattt caatttatga cgaagttcag atttcttctc
      661 ctccagttca cttttgacta gagctctggc tgcagaagct tactagctag ccggcacccc
      721 gtgaatgccc accttcctct tcttcttggg ggccatcttg tcatcgtcat ccttgtaatc
      781 gatgtcatga tctttataat caccgtcatg gtctttgtag tccatcctag ggccgggatt
      841 ctcctccacg tcaccgcatg ttagaagact tcctctgccc tctccgccgc cggacctgaa
      901 attgatttca ccattgttga acttgcggcg
//
