LOCUS       sequence-373000-       11152 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression plasmid of human ZMYND8 fused with FKBP12G36V 
            for dTAG-mediated degradation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11152)
  AUTHORS   Cao Z, Budinich KA, Huang H, Ren D, Lu B, Zhang Z, Chen Q, Zhou Y, 
            Huang YH, Alikarami F, Kingsley MC, Lenard AK, Wakabayashi A, 
            Khandros E, Bailis W, Qi J, Carroll MP, Blobel GA, Faryabi RB, Bernt
            KM, Berger SL, Shi J
  TITLE     ZMYND8-regulated IRF8 transcription axis is an acute myeloid 
            leukemia dependency.
  JOURNAL   Mol Cell. 2021 Sep 2;81(17):3604-3622.e10. doi: 
            10.1016/j.molcel.2021.07.018. Epub 2021 Aug 5.
  PUBMED    34358447
REFERENCE   2  (bases 1 to 11152)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2021.07.018"; journalName: "Mol Cell"; date: 
            "2021-09-2- 2"; volume: "81"; issue: "17"; pages: "3604-3622.e10"
COMMENT     Sequence Label: ZMYND8-dTAG
FEATURES             Location/Qualifiers
     source          1..11152
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1723..1840
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1909..2120
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      2140..2149
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2149..2172
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             5743..6063
                     /codon_start=1
                     /product="F36V mutant of FK506-binding protein FKBP12"
                     /label=FKBP-V
                     /note="binds synthetic ligands that do not bind wild-type 
                     FKBP"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             6070..6096
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             6070..6096
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             6100..6126
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             6142..6198
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             6193..6993
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="GPMIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQG
                     RPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEE
                     HQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQD
                     IALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6253..6272)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6863..6882
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    7009..7597
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7062..7082)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7480..7491)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7669..7902
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7980..8101
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8017..8036)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8071..8090
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8141..8276
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8296..8315)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8297..8315)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8325..8342)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8325..8341)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8334..8356)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8483..8938
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8570..8589)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8780..8801
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8964..9068
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9069..9137,9138..9929)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9287..9306)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10100..10688
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10589..10608
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10842..10859
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10976..10997
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11012..11017,11018..11035,11036..11042)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11050..11066
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11055..11077
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11074..11090
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11074..11090
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11109..11129
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11111..11129
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccagaccac aattttaaaa gaaaaggggg
     1741 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     1801 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     1861 cagagatcca ctttggcgcc ggctcgagtg gctccggtgc ccgtcagtgg gcagagcgca
     1921 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga
     1981 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg
     2041 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg
     2101 ggtttgccgc cagaacacag gtgtcgtgac gcgggatccg ccaccatgga ttacaaagac
     2161 gatgacgata agatgcatcc acagagcttg gctgaagagg aaataaaaac agaacaggag
     2221 gtggtagagg gcatggatat ctctactcgc tccaaagatc ctggctctgc agagagaaca
     2281 gcccagaaaa gaaagttccc cagccctcca cattcttcca atggccactc gccgcaggac
     2341 acatcaacaa gccccattaa aaagaaaaag aaacctggct tactgaacag taacaataag
     2401 gagcagtcag aactaagaca tggtccgttt tactatatga agcagccact caccacagac
     2461 cctgttgatg ttgtaccgca ggatggacgg aatgatttct actgctgggt ttgtcaccgg
     2521 gaaggccaag tcctttgctg tgagctctgt ccccgggttt atcacgctaa gtgtctgaga
     2581 ctgacatcgg aaccagaggg ggactggttt tgtcctgaat gtgagaaaat tacagtagca
     2641 gaatgcatcg agacccagag taaagccatg acaatgctca ccattgaaca gttaagttac
     2701 ctgctcaagt ttgccattca gaaaatgaaa cagccaggga cagatgcatt ccagaagccc
     2761 gttccattgg aacagcaccc tgactatgcg gaatacatct tccatccaat ggatttgtgc
     2821 acccttgaaa agaatgcgaa aaagaaaatg tatggctgca cagaagcctt cctggctgat
     2881 gcaaagtgga ttttgcacaa ctgcatcatt tataatgggg gaaatcacaa attgacgcaa
     2941 atagcgaaag tagtcatcaa aatctgtgaa catgagatga atgaaatcga agtatgtcca
     3001 gaatgttatc tagctgcttg ccaaaaacga gataactggt tttgtgagcc ttgtagcaat
     3061 ccacatcctt tggtctgggc caaactgaag gggtttccat tctggcctgc aaaagctcta
     3121 agggataaag acgggcaggt cgatgcccga ttctttggac aacatgacag ggcctgggtt
     3181 ccaataaata attgctacct catgtctaaa gaaattcctt tttctgtgaa aaagactaag
     3241 agcatcttca acagtgccat gcaagagatg gaggtttacg tggagaacat ccgcaggaag
     3301 tttggggttt ttaattactc tccatttagg acaccctaca cacccaacag ccagtatcaa
     3361 atgctgctcg atcccaccaa ccccagcgcc ggcactgcca agatagacaa gcaggagaag
     3421 gtcaagctca actttgacat gacggcatcc cccaagatcc tgatgagcaa gcctgtgctg
     3481 agtgggggca caggccgccg gatttccttg tcggatatgc cgcgctcccc catgagcaca
     3541 aactcttctg tgcacacggg ctccgacgtg gagcaggatg ctgagaagaa ggccacgtcg
     3601 agccacttca gtgcgagcga ggagtccatg gacttcctgg ataagagcac agcttcacca
     3661 gcctccacca agacgggaca agcagggagt ttatccggca gcccaaagcc cttctctcct
     3721 caactgtcag ctcctatcac gacgaaaacg gacaaaacct ccaccaccgg cagcatcctg
     3781 aatcttaacc tggatcgaag caaagctgag atggatttga aggagctgag cgagtcggtc
     3841 cagcaacagt ccacccctgt tcctctcatc tctcccaagc gccagattcg tagcaggttc
     3901 cagctgaatc ttgacaagac catagagagt tgcaaagcac aattaggcat aaatgaaatc
     3961 tcggaagatg tctatacggc cgtagagcac agcgattcgg aggattctga gaagtcagat
     4021 agtagcgata gtgagtatat cagtgatgat gagcagaagt ctaagaacga gccagaagac
     4081 acagaggaca aagaaggttg tcagatggac aaagagccat ctgctgttaa aaaaaagccc
     4141 aagcctacaa acccagtgga gattaaagag gagctgaaaa gcacgtcacc agccagcgag
     4201 aaggcagacc ctggagcagt caaggacaag gccagccctg agcctgagaa ggacttttcc
     4261 gaaaaggcaa aaccttcacc tcaccccata aaggataaac tgaagggaaa agatgagacg
     4321 gattccccaa cagtccattt gggcctggac tctgattcag agagcgaact tgtcatagat
     4381 ttaggagaag accattctgg gcgggagggt cgaaaaaata agaaggaacc caaagaacca
     4441 tctcccaaac aggatgttgt aggtaaaact ccaccatcca cgacggtggg cagccattct
     4501 cccccggaaa caccggtgct cacccgctct tccgcccaaa cttccgcggc tggcgccaca
     4561 gccaccacca gcacgtcctc cacggtcacc gtcacggccc cggcccccgc cgccacagga
     4621 agcccagtga aaaagcagag gccgctttta ccgaaggaga ctgccccggc cgtgcagcgg
     4681 gtcgtgtgga actcatcaac tgtccagcag aaggagatca cacagagccc atccacgtcc
     4741 accatcaccc tggtgaccag cacacagtca tcgcccctgg tcaccagctc ggggtccatg
     4801 agcacccttg tgtcctcagt caacgctgac ctgcccatcg ccactgcctc agctgatgtc
     4861 gccgctgata ttgccaagta cactagcaaa atgatggatg caataaaagg aacaatgaca
     4921 gaaatataca acgatctttc taaaaacact actggaagca caatagctga gattcgcagg
     4981 ctgaggatcg agatagagaa gctccagtgg ctgcaccagc aagagctctc cgaaatgaaa
     5041 cacaacttag agctgaccat ggcggagatg cggcagagcc tggagcagga gcgggaccgg
     5101 ctcatcgccg aggtgaagaa gcagctggag ttggagaagc agcaggcggt ggatgagacc
     5161 aagaagaagc agtggtgcgc caactgcaag aaggaggcca tcttttactg ctgttggaac
     5221 accagctact gtgactaccc ctgccagcaa gcccactggc ctgagcacat gaagtcctgc
     5281 acccagtcag ctactgctcc tcagcaggaa gcggatgctg aggtgaacac agaaacacta
     5341 aataagtcct cccaggggag ctcctcgagc acacaatcag caccttcaga aacggccagc
     5401 gcctccaaag agaaggagac gtcagctgag aaaagcaagg agagtggctc gacccttgac
     5461 ctttctggct ccagagagac gccctcctcc attctcttag gctccaacca aggctctgtt
     5521 agcaaaaggt gtgacaagca acctgcctat gccccaacca ccacagacca ccagccgcac
     5581 cccaactacc ccgcccagaa gtaccattcc cggagtaata aatccagttg gagcagcagt
     5641 gatgagaaga ggggatcgac acgttccgat cacaacacca gtaccagcac gaagagcctc
     5701 ctcccgaaag agtctcggct ggacaccttc tgggacgcta gcggagtgca ggtggaaacc
     5761 atctccccag gagacgggcg caccttcccc aagcgcggcc agacctgcgt ggtgcactac
     5821 accgggatgc ttgaagatgg aaagaaagtt gattcctccc gggacagaaa caagcccttt
     5881 aagtttatgc taggcaagca ggaggtgatc cgaggctggg aagaaggggt tgcccagatg
     5941 agtgtgggtc agagagccaa actgactata tctccagatt atgcctatgg tgccactggg
     6001 cacccaggca tcatcccacc acatgccact ctcgtcttcg atgtggagct tctaaaactg
     6061 gaaggcggct acccctacga cgtgcccgac tacgccggct atccgtatga tgtcccggac
     6121 tatgcaggca gcggcagcgg cgccaccaac ttcagcctgc tgaagcaggc cggcgacgtg
     6181 gaggagaacc ccggccccat gattgaacaa gatggattgc acgcaggttc tccggccgct
     6241 tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc
     6301 gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc
     6361 ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc
     6421 gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg
     6481 ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc
     6541 atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac
     6601 caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat
     6661 caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc
     6721 aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg
     6781 aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg
     6841 gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc
     6901 gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc
     6961 gccttctatc gccttcttga cgagttcttc tgaacgcgtt aagtcgacaa tcaacctctg
     7021 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     7081 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     7141 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     7201 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     7261 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     7321 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     7381 aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc
     7441 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     7501 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     7561 cagacgagtc ggatctccct ttgggccgcc tccccgcgtc gactttaaga ccaatgactt
     7621 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa
     7681 ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc
     7741 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     7801 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     7861 gatccctcag acccttttag tcagtgtgga aaatctctag cagtacgtat agtagttcat
     7921 gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga
     7981 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     8041 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     8101 atcatgtctg gctctagcta tcccgcccct aactccgccc atcccgcccc taactccgcc
     8161 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     8221 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     8281 gacgtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta
     8341 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
     8401 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
     8461 cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     8521 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     8581 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     8641 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     8701 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     8761 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     8821 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     8881 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag
     8941 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     9001 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     9061 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     9121 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     9181 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     9241 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     9301 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     9361 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     9421 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     9481 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     9541 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     9601 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     9661 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     9721 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     9781 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     9841 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     9901 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     9961 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
    10021 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
    10081 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
    10141 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
    10201 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
    10261 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
    10321 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
    10381 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
    10441 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
    10501 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
    10561 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
    10621 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
    10681 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
    10741 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
    10801 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
    10861 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
    10921 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
    10981 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
    11041 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
    11101 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctgcaa gc
//
