LOCUS       sequence-373018-        4734 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4734)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4734)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SV2C_STOP
FEATURES             Location/Qualifiers
     source          1..4734
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2291..2390)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2407..2426)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2408..2426)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2431..2447)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2431..2447)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2549..2568)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2560..3369
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2627..2646)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3516..4104
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4005..4024
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4258..4275
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4434..4461)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4553..4639)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4688..4710
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4702..4719
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4703..4719
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatgga ggatagctac
      121 aaggacagga cctccctgat gaagggcgcc aaggatatcg cccgcgaggt gaagaagcag
      181 accgtgaaga aggtgaacca ggccgtggat cgggcccagg acgagtatac acagcggagc
      241 tactccagat tccaggacga ggaggacgat gacgattact atccagcagg agagacctat
      301 aacggagagg caaatgacga tgagggaagc tccgaggcaa cagagggaca cgatgaggac
      361 gatgagatct atgagggcga gtaccagggc atcccaagca tgaatcaggc caaggactct
      421 atcgtgagcg tgggccagcc caagggcgat gagtacaagg acaggagaga gctggagtcc
      481 gagaggaggg cagatgagga ggagctggca cagcagtatg agctgatcat ccaggagtgc
      541 ggccacggca gattccagtg ggccctgttc tttgtgctgg gcatggccct gatggccgat
      601 ggcgtggagg tgttcgtggt gggctttgtg ctgcctagcg ccgagaccga cctgtgcatc
      661 ccaaactctg gcagcggctg gctgggctcc atcgtgtacc tgggcatgat ggtgggagcc
      721 ttcttttggg gaggactggc agacaaagtg ggaaggaagc agtctctgct gatctgcatg
      781 agcgtgaatg gcttctttgc cttcctgtct agctttgtgc agggctacgg cttctttctg
      841 ttttgtaggc tgctgagcgg attcggaatc ggaggagcca tccctaccgt gttctcctat
      901 tttgcagagg tgctggcaag ggagaagaga ggagagcacc tgtcttggct gtgcatgttt
      961 tggatgatcg gaggaatcta cgcaagcgcc atggcatggg caatcatccc ccactatggc
     1021 tggtccttct ctatgggctc cgcctaccag tttcactctt ggcgggtgtt cgtgatcgtg
     1081 tgcgccctgc cttgcgtgag ctctgtggtg gccctgacct ttatgcccga gagccctaga
     1141 ttcctgctgg aagtgggcaa gcacgatgag gcctggatga tcctgaagct gatccacgac
     1201 acaaacatgc gggccagagg ccagcccgag aaggtgttta ccgtgaataa gatcaagaca
     1261 cctaagcaga tcgatgagct gatcgagatc gagtccgaca ccggcacatg gtatcggaga
     1321 tgcttcgtga ggatccgcac cgagctgtac ggcatctggc tgacattcat gcggtgtttt
     1381 aactatccag tgagagacaa taccatcaag ctgacaatcg tgtggttcac cctgtctttt
     1441 ggctactatg gcctgagcgt gtggttccca gatgtgatca agcccctgca gagcgacgag
     1501 tacgccctgc tgaccaggaa cgtggagcgc gataagtatg ccaacttcac catcaacttc
     1561 accatggaga accagatcca cacaggcatg gagtacgaca atggcaggtt catcggcgtg
     1621 aagtttaagt ctgtgacctt caaggatagc gtgttcaaga gctgcacatt cgaggacgtg
     1681 acctccgtga acacatactt taagaattgt accttcatcg atacagtgtt tgataacacc
     1741 gacttcgagc catataagtt tatcgacagc gagttcaaga actgctcctt ctttcacaat
     1801 aagaccggct gtcagatcac atttgacgat gactacagcg cctattggat ctacttcgtg
     1861 aactttctgg gcacactggc cgtgctgccc ggcaatatcg tgtccgccct gctgatggat
     1921 aggatcggcc gcctgaccat gctgggagga tctatggtgc tgtccggcat ctcttgcttc
     1981 tttctgtggt tcggcacaag cgagtccatg atgatcggca tgctgtgcct gtacaacggc
     2041 ctgaccatct ccgcctggaa ttctctggat gtggtgacag tggagctgta tcctaccgac
     2101 aggagggcaa caggattcgg ctttctgaac gcactgtgca aggcagcagc cgtgctgggc
     2161 aatctgatct tcggcagcct ggtgtccatc accaagtcca tccctatcct gctggcctct
     2221 acagtgctgg tgtgcggagg actggtggga ctgtgcctgc cagacaccag aacacaggtg
     2281 ctgatgtaag acccagcttt cttgtacaaa gttggcatta taagaaagca ttgcttatca
     2341 atttgttgca acgaacaggt cactatcagt caaaataaaa tcattatttg ccatccagct
     2401 gatatcccct atagtgagtc gtattacatg gtcatagctg tttcctggca gctctggccc
     2461 gtgtctcaaa atctctgatg ttacattgca caagataaaa atatatcatc atgaacaata
     2521 aaactgtctg cttacataaa cagtaataca aggggtgtta tgagccatat tcaacgggaa
     2581 acgtcgaggc cgcgattaaa ttccaacatg gatgctgatt tatatgggta taaatgggct
     2641 cgcgataatg tcgggcaatc aggtgcgaca atctatcgct tgtatgggaa gcccgatgcg
     2701 ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca atgatgttac agatgagatg
     2761 gtcagactaa actggctgac ggaatttatg cctcttccga ccatcaagca ttttatccgt
     2821 actcctgatg atgcatggtt actcaccact gcgatccccg gaaaaacagc attccaggta
     2881 ttagaagaat atcctgattc aggtgaaaat attgttgatg cgctggcagt gttcctgcgc
     2941 cggttgcatt cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc
     3001 gctcaggcgc aatcacgaat gaataacggt ttggttgatg cgagtgattt tgatgacgag
     3061 cgtaatggct ggcctgttga acaagtctgg aaagaaatgc ataaactttt gccattctca
     3121 ccggattcag tcgtcactca tggtgatttc tcacttgata accttatttt tgacgagggg
     3181 aaattaatag gttgtattga tgttggacga gtcggaatcg cagaccgata ccaggatctt
     3241 gccatcctat ggaactgcct cggtgagttt tctccttcat tacagaaacg gctttttcaa
     3301 aaatatggta ttgataatcc tgatatgaat aaattgcagt ttcatttgat gctcgatgag
     3361 tttttctaat cagaattggt taattggttg taacactggc agagcattac gctgacttga
     3421 cgggacggcg caagctcatg accaaaatcc cttaacgtga gttacgcgtc gttccactga
     3481 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     3541 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     3601 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     3661 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     3721 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     3781 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     3841 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     3901 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     3961 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     4021 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     4081 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     4141 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     4201 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     4261 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
     4321 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
     4381 cgcaacgcaa ttaatacgcg taccgctagc caggaagagt ttgtagaaac gcaaaaaggc
     4441 catccgtcag gatggccttc tgcttagttt gatgcctggc agtttatggc gggcgtcctg
     4501 cccgccaccc tccgggccgt tgcttcacaa cgttcaaatc cgctcccggc ggatttgtcc
     4561 tactcaggag agcgttcacc gacaaacaac agataaaacg aaaggcccag tcttccgact
     4621 gagcctttcg ttttatttga tgcctggcag ttccctactc tcgcgttaac gctagcatgg
     4681 atgttttccc agtcacgacg ttgtaaaacg acggccagtc ttaagctcgg gccc
//
