LOCUS       sequence-37303-e         866 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Piatkevich KD, Hulit J, Subach OM, Wu B, Abdulla A, Segall JE, 
            Verkhusha VV
  TITLE     Monomeric red fluorescent proteins with a large Stokes shift.
  JOURNAL   Proc Natl Acad Sci U S A. 2010 Mar 23;107(12):5369-74. Epub 2010 Mar
            8.
  PUBMED    20212155
REFERENCE   2  (bases 1 to 866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2010-03-23"; volume: "107(12)"; pages: 
            "5369-74. Epub 2010 Mar 8"
COMMENT     Sequence Label: pBAD/HisD-LSSmKate1
FEATURES             Location/Qualifiers
     source          1..866
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             68..85
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(842..859)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
ORIGIN
        1 tcgcaactct ctactgtttc tccatacccg ttttttgggc taacaggagg aattaaccat
       61 ggggagccac catcaccatc accatggcag atctatgagc gagctgatta aggagaacat
      121 gcacatgaag ctgtacatgg aaggcaccgt gaacaaccac cacttcaagt gcacatccga
      181 gggcgaaggc aagccctacg agggcaccca gaccatgaga atcaaggtgg tcgagggcgg
      241 ccctctaccc ttcgccttcg acatcttggc taccagcttc atgtacggca gctacacctt
      301 catcaaccac acccagggca tccccgactt ctttaagcag tccttccctg agggcttcac
      361 atgggagaga gtcaccacat acgaagacgg gggcgtgctg accgctaccc aggacaccag
      421 cctccaggac ggttgcctca tctacaacgt caagatcaga ggggtgaact tcacatccaa
      481 cggccctgtg atgcagaaga aaacactcgg ctgggaggcc gggaccgaga tgctgtaccc
      541 cgctgacggc ggcctggaag gcagaagcga cgaggccctg aagctcgtgg gcgggggcca
      601 cctgatctgc aacttgaaga gcacatacag atccaagaaa cccgctaaga atctcaaggt
      661 gcccggcgtc tactatgtgg accgaagact ggaaagaatc aaggaggccg acaaagagac
      721 ctacgtcgag cagcacgagg tggctgtggc cagatactgc gacctcccta gcaaactggg
      781 gcacaagctt aattaagaat tcgaagcttg gctgttttgg tggatgagag aagattttca
      841 gcctgataca gattaaatca gaacgc
//
