LOCUS       sequence-373041-        6872 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses GLPLight1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6872)
  AUTHORS   Duffet L, Williams ET, Gresch A, Chen S, Bhat MA, Benke D, Hartrampf
            N, Patriarchi T
  TITLE     Optical tools for visualizing and controlling human GLP-1 receptor 
            activation with high spatiotemporal resolution.
  JOURNAL   Elife. 2023 Jun 2;12:RP86628. doi: 10.7554/eLife.86628.
  PUBMED    37265064
REFERENCE   2  (bases 1 to 6872)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2023-06-2"; volume: "12:RP86628. doi"; pages: " 
            10.7554/eLife.86628"
COMMENT     Sequence Label: AAV.CAG.GLPLight1
FEATURES             Location/Qualifiers
     source          1..6872
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
     promoter        441..718
                     /label=chicken beta-actin promoter
     primer_bind     997..1019
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1081..1100
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     2379..2400
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(2512..2533)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2773..2792)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     misc_feature    3316..3904
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3369..3389)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3787..3798)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3947..4068
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3958..3977)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4012..4031
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   4230..4359
                     /label=AAV2 ITR
     rep_origin      complement(4596..5184)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4676..4695)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5355..6146,6147..6215))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5978..5997
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6216..6320)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(6346..6801)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6483..6504)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6695..6714
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct tgtagttaat gattaacccg ccatgctact tatctacgta gccatgctct
      181 aggaagagta ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
      241 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
      301 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
      361 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
      421 agtcatcgct attaccatgg tcgaggtgag ccccacgttc tgcttcactc tccccatctc
      481 ccccccctcc ccacccccaa ttttgtattt atttattttt taattatttt gtgcagcgat
      541 gggggcgggg gggggggggg ggcgcgcgcc aggcggggcg gggcggggcg aggggcgggg
      601 cggggcgagg cggagaggtg cggcggcagc caatcagagc ggcgcgctcc gaaagtttcc
      661 ttttatggcg aggcggcggc ggcggcggcc ctataaaaag cgaagcgcgc ggcgggcggg
      721 agtcgctgcg cgctgccttc gccccgtgcc ccgctccgcc gccgcctcgc gccgcccgcc
      781 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc
      841 gggctgtaat tagcgcttgg tttaatgacg gcttgtttct tttctgtggc tgcgtgaaag
      901 ccttgagggg ctccgggagg gccctttgtg cggggggagc ggctcggggc tgtccgcggg
      961 gggacggctg ccttcggggg ggacggggca gggcggggtt cggcttctgg cgtgtgaccg
     1021 gcggctctag agcctctgct aaccatgttc atgccttctt ctttttccta cagctcctgg
     1081 gcaacgtgct ggttattgtg ctgtctcatc attttggcaa agaattggat ccggtacctg
     1141 cgcgccatga agacgatcat cgccctgagc tacatcttct gcctggtgtt cgccgactac
     1201 aaggacgatg atgacgccag gccgcaaggc gcgaccgttt cactgtggga gactgtgcaa
     1261 aagtggcgag agtacagacg gcagtgtcag cgatcactta cagaagatcc accgcctgca
     1321 accgatctct tctgcaaccg caccttcgac gagtacgcgt gttggccaga tggggagccc
     1381 ggcagtttcg taaacgtgag ctgtccgtgg tatctgcctt gggcttctag tgtaccgcaa
     1441 ggacacgtgt accgattttg tactgctgag gggctgtggc tccaaaagga taatagtagc
     1501 cttccgtggc gggatctgtc agaatgcgaa gaaagcaaac gcggtgaacg aagcagtcct
     1561 gaggaacaac ttcttttttt gtatataatc tatacagtag ggtatgctct gtctttttca
     1621 gccctcgtga ttgcttctgc tatactcttg ggatttcgcc accttcactg cacacgcaat
     1681 tatattcatc ttaatctctt cgcgtcattc atactccggg ctctgagcgt tttcattaag
     1741 gacgcggcac tgaaatggat gtatagtacg gctgcacaac aacaccaatg ggacggtctg
     1801 ctctcttacc aagatagcct ttcctgccgc ctcgtgttcc tcttgatgca atattgcgtt
     1861 gcagcaaatt attactggct ccttgtcgaa ggggtctacc tgtatacgtt gcttgctttt
     1921 tccgtcaaat ccgagcagtg gatttttaga ttgtatgtga gcattggttg gggcgttcct
     1981 ttgcttttcg ttgtgccgtg gggtattgta aagtacctgt acgaggacga ggggtgttgg
     2041 actcgcaaca gcaacatgaa ctactggctg attattagac ttccgattct ctttgccatt
     2101 ggagtaaact tccttatttt tgttcgagtg atctgtattg tagtaagcaa actttatctg
     2161 agctcactca ttaacgtcta tatcaaggcc gacaagcaga agaacggcat caaggcgaac
     2221 ttcaagatcc gccacaacat cgaggacggc ggcgtgcagc tcgcctacca ctaccagcag
     2281 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcgtgcag
     2341 tccaaacttt cgaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     2401 accgccgccg ggatcactct cggcatggac gagctgtaca agggcggtac cggagggcaa
     2461 gaaagcatga gcagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
     2521 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggtga gggcgatgcc
     2581 accacaggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg
     2641 cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac
     2701 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacatcca ggagcgcacc
     2761 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac
     2821 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
     2881 gggcacaagc tggagtacaa caatcatgac caactgaatg acatcaagtg tagacttgca
     2941 aagtctaccc tcacgctgat accacttctg ggtacgcatg aagtgatctt tgctttcgta
     3001 atggatgaac atgcccgcgg caccttgaga ttcataaagc tgttcacaga attgagtttt
     3061 acgagttttc aaggcctcat ggtggcgatc ctctactgtt ttgtgaacaa tgaggtccag
     3121 ctggagttcc gcaagagttg ggaaaggtgg cggcttgagc atctgcatat ccagcgggat
     3181 gcagcaatga aaccattgaa gtgcccggca gcggctctgg cggctggagc ggctgctgga
     3241 agtagtatgt atactgccac ctgccaagca agttgcagtt tctgctacga aaacgaagta
     3301 tgaaagctta tcgataatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     3361 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     3421 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     3481 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     3541 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     3601 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     3661 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc
     3721 tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     3781 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     3841 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     3901 ccgcatcgat accgtcgacc cgggcggccg cttcgagcag acatgataag atacattgat
     3961 gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt
     4021 gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat
     4081 tgcattcatt ttatgtttca ggttcagggg gagatgtggg aggtttttta aagcaagtaa
     4141 aacctctaca aatgtggtaa aatcgataag gatcttccta gagcatggct acgtagataa
     4201 gtagcatggc gggttaatca ttaactacaa ggaaccccta gtgatggagt tggccactcc
     4261 ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc gacgcccggg
     4321 ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc tgcattaatg aatcggccaa
     4381 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     4441 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     4501 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     4561 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     4621 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     4681 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     4741 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     4801 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     4861 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     4921 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     4981 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     5041 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     5101 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     5161 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     5221 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     5281 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     5341 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     5401 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     5461 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     5521 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     5581 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     5641 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     5701 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     5761 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     5821 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     5881 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     5941 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     6001 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     6061 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     6121 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     6181 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     6241 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     6301 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctaaatt gtaagcgtta
     6361 atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg
     6421 ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg
     6481 ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa
     6541 aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg
     6601 ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt
     6661 gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg
     6721 ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta
     6781 atgcgccgct acagggcgcg tcccattcgc cattcaggct gcgcaactgt tgggaagggc
     6841 gatcggtgcg ggcctcttcg ctattacgcc ag
//
