LOCUS       sequence-373042-        6600 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses GLPLight-ctr in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6600)
  AUTHORS   Duffet L, Williams ET, Gresch A, Chen S, Bhat MA, Benke D, Hartrampf
            N, Patriarchi T
  TITLE     Optical tools for visualizing and controlling human GLP-1 receptor 
            activation with high spatiotemporal resolution.
  JOURNAL   Elife. 2023 Jun 2;12:RP86628. doi: 10.7554/eLife.86628.
  PUBMED    37265064
REFERENCE   2  (bases 1 to 6600)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2023-06-2"; volume: "12:RP86628. doi"; pages: " 
            10.7554/eLife.86628"
COMMENT     Sequence Label: AAV.hSyn.GLPLight-ctr
FEATURES             Location/Qualifiers
     source          1..6600
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        164..611
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     intron          635..767
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     750..769
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     2042..2063
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(2175..2196)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2436..2455)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     misc_feature    2979..3567
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3032..3052)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3450..3461)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3585..3809
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   3852..3992
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3852..3981
                     /label=AAV2 ITR
     rep_origin      4067..4522
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4154..4173)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4364..4385
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4539..4558)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4658..4680
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4718..4736)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4804..4908
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4909..4977,4978..5769)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5127..5146)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5940..6528
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6429..6448
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   join(6590..6600,1..119)
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
ORIGIN
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcgtcgg gcgacctttg gtcgcccggc
       61 ctcagtgagc gagcgagcgc gcagagaggg agtggccaac tccatcacta ggggttcctg
      121 cggccgcacg cgtgtgtcta gactgcagag ggccctgcgt atgagtgcaa gtgggtttta
      181 ggaccaggat gaggcggggt gggggtgcct acctgacgac cgaccccgac ccactggaca
      241 agcacccaac ccccattccc caaattgcgc atcccctatc agagaggggg aggggaaaca
      301 ggatgcggcg aggcgcgtgc gcactgccag cttcagcacc gcggacagtg ccttcgcccc
      361 cgcctggcgg cgcgcgccac cgccgcctca gcactgaagg cgcgctgacg tcactcgccg
      421 gtcccccgca aactcccctt cccggccacc ttggtcgcgt ccgcgccgcc gccggcccag
      481 ccggaccgca ccacgcgagg cgcgagatag gggggcacgg gcgcgaccat ctgcgctgcg
      541 gcgccggcga ctcagcgctg cctcagtctg cggtgggcag cggaggagtc gtgtcgtgcc
      601 tgagagcgca gtcgagatta attaactcga gcaggtaagt atcaaggtta caagacaggt
      661 ttaaggagac caatagaaac tgggcttgtc gagacagaga agactcttgc gtttctgata
      721 ggcacctatt ggtcttactg acatccactt tgcctttctc tccacaggtg ctagcgttta
      781 aacggtaccg gatccggtac ctgcgcgcca tgaagacgat catcgccctg agctacatct
      841 tctgcctggt gttcgccgac tacaaggacg atgatgacgc caggccgcaa ggcgcgaccg
      901 tttcactgtg ggagactgtg caaaagtggc gagagtacag acggcagtgt cagcgatcac
      961 ttacagaaga tccaccgcct gcaaccgatc tcttctgcaa ccgcaccttc gacgagtacg
     1021 cgtgttggcc agatggggag cccggcagtt tcgtaaacgt gagctgtccg tggtatctgc
     1081 cttgggcttc tagtgtaccg caaggacacg tgtaccgatt ttgtactgct gaggggctgt
     1141 ggctccaaaa ggataatagt agccttccgt ggcgggatct gtcagaatgc gaagaaagca
     1201 aacgcggtga acgaagcagt cctgaggaac aagcactttt tttgtatata atctatacag
     1261 tagggtatgc tctgtccttt tcagccctcg tgattgcttc tgctatactc ttgggatttc
     1321 gccaccttca ctgcacacgc aattatattc atcttaatct cttcgcgtca ttcatactcc
     1381 gggctctgag cgttttcatt aaggacgcgg cactgaaatg gatgtatagt acggctgcac
     1441 aacaacacca atgggacggt ctgctctctt accaagatag cctttcctgc cgcctcgtgt
     1501 tcctcttgat gcaatattgc gttgcagcaa attattactg gctccttgtc gaaggggtct
     1561 acctgtatac gttgcttgct ttttccgtca aatccgagca gtggattttt agattgtatg
     1621 tgagcattgg ttggggcgtt cctttgcttt tcgttgtgcc gtggggtatt gtaaagtacc
     1681 tgtacgagga cgaggggtgt tggactcgcg caagcaacat gaactactgg ctgattatta
     1741 gacttccgat tctctttgcc attggagtaa acttccttat ttttgttcga gtgatctgta
     1801 ttgtagtaag caaactttat ctgagctcac tcattaacgt ctatatcaag gccgacaagc
     1861 agaagaacgg catcaaggcg aacttcaaga tccgccacaa catcgaggac ggcggcgtgc
     1921 agctcgccta ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
     1981 acaaccacta cctgagcgtg cagtccaaac tttcgaaaga ccccaacgag aagcgcgatc
     2041 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt
     2101 acaagggcgg taccggaggg caagaaagca tgagcagcaa gggcgaggag ctgttcaccg
     2161 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt
     2221 ccggcgaggg tgagggcgat gccaccacag gcaagctgac cctgaagttc atctgcacca
     2281 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt
     2341 gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg
     2401 aaggctacat ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg
     2461 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact
     2521 tcaaggagga cggcaacatc ctggggcaca agctggagta caacaatcat gaccaactga
     2581 atgacatcaa gtgtagactt gcaaagtcta ccctcacgct gataccactt ctgggtacgc
     2641 atgaagtgat ctttgctttc gtaatggatg aacatgcccg cggcaccttg agattcataa
     2701 agctgttcac agcattgagt tttacgagtt ttcaaggcct catggtggcg atcctctact
     2761 gttttgtgaa caatgaggtc cagctggagt tccgcaagag ttgggaaagg tggcggcttg
     2821 agcatctgca tatccagcgg gatgcagcaa tgaaaccatt gaagtgcccg gcagcggctc
     2881 tggcggctgg agcggctgct ggaagtagta tgtatactgc cacctgccaa gcaagttgca
     2941 gtttctgcta cgaaaacgaa gtatgaaagc ttatcgataa tcaacctctg gattacaaaa
     3001 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
     3061 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
     3121 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
     3181 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
     3241 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
     3301 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     3361 tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc
     3421 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
     3481 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
     3541 ggatctccct ttgggccgcc tccccgctct cgagatacgt acgactgtgc cttctagttg
     3601 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc
     3661 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc
     3721 tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag
     3781 gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagatctc acgtgcggac
     3841 cgagcggccg caggaacccc tagtgatgga gttggccact ccctctctgc gcgctcgctc
     3901 gctcactgag gccgggcgac caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc
     3961 agtgagcgag cgagcgcgca gctgcctgca ggggcgcctg atgcggtatt ttctccttac
     4021 gcatctgtgc ggtatttcac accgcatacg tcaaagcaac catagtacgc gccctgtagc
     4081 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     4141 gccttagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     4201 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     4261 ctcgacccca aaaaacttga tttgggtgat ggttcacgta gtgggccatc gccctgatag
     4321 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     4381 actggaacaa cactcaactc tatctcgggc tattcttttg atttataagg gattttgccg
     4441 atttcggtct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
     4501 aaaatattaa cgtttacaat tttatggtgc actctcagta caatctgctc tgatgccgca
     4561 tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg
     4621 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg
     4681 ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta
     4741 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
     4801 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     4861 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     4921 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     4981 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     5041 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     5101 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     5161 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     5221 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     5281 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     5341 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     5401 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     5461 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     5521 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     5581 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     5641 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     5701 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     5761 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     5821 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     5881 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     5941 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     6001 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     6061 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     6121 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     6181 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     6241 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     6301 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     6361 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     6421 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     6481 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     6541 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtc ctgcaggcag
//
