LOCUS       sequence-37305-e         881 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Subach FV, Zhang L, Gadella TW, Gurskaya NG, Lukyanov KA, Verkhusha 
            VV
  TITLE     Red fluorescent protein with reversibly photoswitchable absorbance 
            for photochromic FRET.
  JOURNAL   Chem Biol. 2010 Jul 30;17(7):745-55.
  PUBMED    20659687
REFERENCE   2  (bases 1 to 881)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Chem 
            Biol."; date: "2010-07-30"; volume: "17(7)"; pages: "745-55"
COMMENT     Sequence Label: pBAD/HisD-rsTagRFP
FEATURES             Location/Qualifiers
     source          1..881
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             69..86
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             96..797
                     /codon_start=1
                     /product="far-red monomeric fluorescent protein with an 
                     emission peak at 657 nm (Morozova et al., 2010)"
                     /label=TagRFP657
                     /note="derived from mKate"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFWKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKLRGVNFPSNGPVMQKKTLGWEAATEMLYPADGGLEGRGDMALK
                     LVGGGHLICNLKTTYRSKNPAKNLKMPGVYFVDHRLERIKEADKETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     primer_bind     complement(843..860)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
ORIGIN
        1 tcgcaactct ctactgtttc tccatacccg tttttttggg ctaacaggag gaattaacca
       61 tggggagcca ccatcaccat caccatggca gatctatgag cgagctgatt aaggagaaca
      121 tgcacatgaa gctgtacatg gagggcaccg tgaacaacca ccacttcaag tgcacatccg
      181 agggcgaagg caagccctac gagggcaccc agaccatgag aatcaaggtg gtcgagggcg
      241 gccctctccc cttcgccttc gacatcctgg ctaccagctt catgtacggc agccgcacct
      301 tcatcaacca cacccagggc atccccgact tctggaagca gtccttccct gagggcttca
      361 catgggagag agtcaccaca tacgaagacg ggggcgtgct gaccgctacc caggacacca
      421 gcctccagga cggctgcctc atctacaacg tcaagctcag aggggtgaac ttcccatcca
      481 acggccctgt gatgcagaag aaaacactcg gctgggaggc cgccaccgag atgctgtacc
      541 ccgctgacgg cggcctggaa ggcagagggg acatggccct gaagctcgtg ggcgggggcc
      601 acctgatctg caacttgaag accacataca gatccaagaa tcccgctaag aacctcaaga
      661 tgcccggcgt ctactttgtg gaccacagac tggaaagaat caaggaggcc gacaaagaga
      721 cctacgtcga gcagcacgag gtggctgtgg ccagatactg cgacctccct agcaaactgg
      781 ggcacaagct taattaagaa ttcgaagctt ggctgttttg gtggatgaga gaagattttc
      841 agcctgatac agattaaatc agaacgcaga agcggtctga t
//
