LOCUS       sequence-37306-e         854 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Subach FV, Patterson GH, Renz M, Lippincott-Schwartz J, Verkhusha VV
  TITLE     Bright monomeric photoactivatable red fluorescent protein for 
            two-color super-resolution sptPALM of live cells.
  JOURNAL   J Am Chem Soc. 2010 May 12;132(18):6481-91.
  PUBMED    20394363
REFERENCE   2  (bases 1 to 854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Am Chem 
            Soc."; date: "2010-05-12"; volume: "132(18)"; pages: "6481-91"
COMMENT     Sequence Label: pBAD/HisD-PATagRFP
FEATURES             Location/Qualifiers
     source          1..854
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             68..85
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             95..796
                     /codon_start=1
                     /product="photoactivatable variant of the monomeric red 
                     fluorescent protein TagRFP (Subach et al., 2010)"
                     /label=PA-TagRFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFMYGSSTFINHTQGIPDFWKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFPSNGPVMKKKTLGWEPSTEKLKPADGGLEGRVDMALK
                     LVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDRRLEIIKEADKETYWEQHEVAVARYS
                     DLPSKLGHKLN"
ORIGIN
        1 tcgcaactct ctactgtttc tccatacccg ttttttgggc taacaggagg aattaaccat
       61 ggggagccac catcaccatc accatggcag atctatgagc gagctgatta aggagaacat
      121 gcacatgaag ctgtacatgg agggcaccgt gaacaaccac cacttcaagt gcacatccga
      181 gggcgaaggc aagccctacg agggcaccca gaccatgaga atcaaggtgg tcgagggcgg
      241 ccctctcccc ttcgccttcg acatcctggc taccagcttc atgtacggca gcagcacctt
      301 catcaaccac acccagggca tccccgactt ctggaagcag tccttccctg agggcttcac
      361 atgggagaga gtcaccacat acgaagacgg gggcgtgctg accgctaccc aggacaccag
      421 cctccaggac ggctgcctca tctacaacgt caagatcaga ggggtgaact tcccatccaa
      481 cggccctgtg atgaagaaga aaacactcgg ctgggaaccg agcaccgaga aactgaaacc
      541 cgctgacggc ggcctggaag gcagggtcga catggccctg aagctcgtgg gcgggggcca
      601 cctgatctgc aacttcaaga ccacatacag atccaagaaa cccgctaaga acctcaagat
      661 gcccggcgtc tactatgtgg accgcagact ggaaatcatc aaggaggccg acaaagagac
      721 ctactgggag cagcacgagg tggctgtggc cagatactcc gacctcccta gcaaactggg
      781 gcacaagctt aattaagaat tcgaagcttg gctgttttgg tggatgagag aagattttca
      841 gcctgataca gatt
//
