LOCUS       sequence-373068-         877 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of N-terminally mGFP labelled mutated FGFR3 (isoform 
            IIIc)-K650Q mutation (associated with HCH; target mutation: 
            c.1948A>C; silent mutation added on purpose: c.1938C>T).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Hartl I, Brumovska V, Striedner Y, Yasari A, Schutz GJ, Sevcsik E, 
            Tiemann-Boege I
  TITLE     Measurement of FGFR3 signaling at the cell membrane via total 
            internal reflection fluorescence microscopy to compare the 
            activation of FGFR3 mutants.
  JOURNAL   J Biol Chem. 2023 Feb;299(2):102832. doi: 10.1016/j.jbc.2022.102832.
            Epub 2022 Dec 27.
  PUBMED    36581204
REFERENCE   2  (bases 1 to 877)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2023-02"; volume: "299(2):102832. doi"; pages: " 
            10.1016/j.jbc.2022.102832. Epub 2022 Dec 27"
COMMENT     Sequence Label: pcDNA3.1_mGFP-FGFR3-K650Q
FEATURES             Location/Qualifiers
     source          1..877
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(723..740)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    729..840
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 cttcgacacc tgcaagccgc ccgaggagca gctcaccttc aaggacctgg tgtcctgtgc
       61 ctaccaggtg gcccggggca tggagtactt ggcctcccag aagtgcatcc acagggacct
      121 ggctgcccgc aatgtgctgg tgaccgagga caacgtgatg aagatcgcag acttcgggct
      181 ggcccgggac gtgcacaacc tcgattacta caagcagaca accaacggcc ggctgcccgt
      241 gaagtggatg gcgcctgagg ccttgtttga ccgagtctac actcaccaga gtgacgtctg
      301 gtcctttggg gtcctgctct gggagatctt cacgctgggg ggctccccgt accccggcat
      361 ccctgtggag gagctcttca agctgctgaa ggagggccac cgcatggaca agcccgccaa
      421 ctgcacacac gacctgtaca tgatcatgcg ggagtgctgg catgccgcgc cctcccagag
      481 gcccaccttc aagcagctgg tggaggacct ggaccgtgtc cttaccgtga cgtccaccga
      541 cgagtacctg gacctgtcgg cgcctttcga gcagtactcc ccgggtggcc aggacacccc
      601 cagctccagc tcctcagggg acgactccgt gtttgcccac gacctgctgc ccccggcccc
      661 acccagcagt gggggctcgc ggacgtgata atctagaggg cccgtttaaa cccgctgatc
      721 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
      781 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
      841 gcattgtctg agtaggtgtc attctattct ggggggt
//
