LOCUS       sequence-37307-e         869 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 869)
  AUTHORS   Subach FV, Patterson GH, Renz M, Lippincott-Schwartz J, Verkhusha VV
  TITLE     Bright monomeric photoactivatable red fluorescent protein for 
            two-color super-resolution sptPALM of live cells.
  JOURNAL   J Am Chem Soc. 2010 May 12;132(18):6481-91.
  PUBMED    20394363
REFERENCE   2  (bases 1 to 869)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Am Chem 
            Soc."; date: "2010-05-12"; volume: "132(18)"; pages: "6481-91"
COMMENT     Sequence Label: pBAD/HisD-PATagRFP
FEATURES             Location/Qualifiers
     source          1..869
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(9..710)
                     /codon_start=1
                     /product="photoactivatable variant of the monomeric red 
                     fluorescent protein TagRFP (Subach et al., 2010)"
                     /label=PA-TagRFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFMYGSSTFINHTQGIPDFWKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFPSNGPVMKKKTLGWEPSTEKLKPADGGLEGRVDMALK
                     LVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDRRLEIIKEADKETYWEQHEVAVARYS
                     DLPSKLGHKLN"
     CDS             complement(720..737)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(838..857)
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
ORIGIN
        1 tcgaattctt aattaagctt gtgccccagt ttgctaggga ggtcggagta tctggccaca
       61 gccacctcgt gctgctccca gtaggtctct ttgtcggcct ccttgatgat ttccagtctg
      121 cggtccacat agtagacgcc gggcatcttg aggttcttag cgggtttctt ggatctgtat
      181 gtggtcttga agttgcagat caggtggccc ccgcccacga gcttcagggc catgtcgacc
      241 ctgccttcca ggccgccgtc agcgggtttc agtttctcgg tgctcggttc ccagccgagt
      301 gttttcttct tcatcacagg gccgttggat gggaagttca cccctctgat cttgacgttg
      361 tagatgaggc agccgtcctg gaggctggtg tcctgggtag cggtcagcac gcccccgtct
      421 tcgtatgtgg tgactctctc ccatgtgaag ccctcaggga aggactgctt ccagaagtcg
      481 gggatgccct gggtgtggtt gatgaaggtg ctgctgccgt acatgaagct ggtagccagg
      541 atgtcgaagg cgaaggggag agggccgccc tcgaccacct tgattctcat ggtctgggtg
      601 ccctcgtagg gcttgccttc gccctcggat gtgcacttga agtggtggtt gttcacggtg
      661 ccctccatgt acagcttcat gtgcatgttc tccttaatca gctcgctcat agatctgcca
      721 tggtgatggt gatggtggct ccccatggtt aattcctcct gttagcccaa aaaacgggta
      781 tggagaaaca gtagagagtt gcgataaaaa gcgtcaggta ggatccgcta atcttatgga
      841 taaaaatgct atggcatagc aatggagct
//
